Q9BY15 (EMR3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EGF-like module-containing mucin-like hormone receptor-like 3

Alternative name(s):
EGF-like module receptor 3

Cleaved into the following 2 chains:

1. EGF-like module-containing mucin-like hormone receptor-like 3 subunit alpha
2. EGF-like module-containing mucin-like hormone receptor-like 3 subunit beta

Gene names

Name:	EMR3
ORF Names:	UNQ683/PRO1562

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor probably involved in myeloid interactions during immune and inflammatory responses. A ligand for the soluble form of this receptor is present at the surface of momocytes-derived macrophages and activated neutrophils.
Subunit structure	Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit) By similarity.
Subcellular location	EGF-like module-containing mucin-like hormone receptor-like 3: Cell membrane; Multi-pass membrane protein. EGF-like module-containing mucin-like hormone receptor-like 3 subunit beta: Cell membrane; Multi-pass membrane protein. EGF-like module-containing mucin-like hormone receptor-like 3 subunit alpha: Secreted › extracellular space. Note: A soluble form is also detected (alpha subunit). Isoform 3: Secreted.
Tissue specificity	Displays a predominantly leukocyte-restricted expression, with highest levels in neutrophils, monocytes and macrophages.
Post-translational modification	Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 2 EGF-like domains. Contains 1 GPS domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	EGF-like domain Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Traceable author statement PubMed 15203201. Source: GDB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Traceable author statement PubMed 15203201. Source: GDB calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BY15-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BY15-2)

The sequence of this isoform differs from the canonical sequence as follows:
     67-118: Missing.

Isoform 3 (identifier: Q9BY15-3)

The sequence of this isoform differs from the canonical sequence as follows:
     67-652: Missing.

Note: Soluble form.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 652	631	EGF-like module-containing mucin-like hormone receptor-like 3		PRO_0000012876
	Chain	22 – 338	317	EGF-like module-containing mucin-like hormone receptor-like 3 subunit alpha By similarity		PRO_0000296239
	Chain	339 – 652	314	EGF-like module-containing mucin-like hormone receptor-like 3 subunit beta By similarity		PRO_0000296240
Regions
	Topological domain	22 – 357	336	Extracellular Potential
	Transmembrane	358 – 378	21	Helical; Name=1; Potential
	Topological domain	379 – 389	11	Cytoplasmic Potential
	Transmembrane	390 – 410	21	Helical; Name=2; Potential
	Topological domain	411 – 416	6	Extracellular Potential
	Transmembrane	417 – 437	21	Helical; Name=3; Potential
	Topological domain	438 – 464	27	Cytoplasmic Potential
	Transmembrane	465 – 485	21	Helical; Name=4; Potential
	Topological domain	486 – 508	23	Extracellular Potential
	Transmembrane	509 – 529	21	Helical; Name=5; Potential
	Topological domain	530 – 557	28	Cytoplasmic Potential
	Transmembrane	558 – 578	21	Helical; Name=6; Potential
	Topological domain	579 – 580	2	Extracellular Potential
	Transmembrane	581 – 601	21	Helical; Name=7; Potential
	Topological domain	602 – 652	51	Cytoplasmic Potential
	Domain	24 – 66	43	EGF-like 1
	Domain	67 – 118	52	EGF-like 2; calcium-binding Potential
	Domain	301 – 350	50	GPS
Sites
	Site	338 – 339	2	Cleavage By similarity
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	145	1	N-linked (GlcNAc...) Potential
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Glycosylation	279	1	N-linked (GlcNAc...) Potential
	Glycosylation	327	1	N-linked (GlcNAc...) Potential
	Glycosylation	334	1	N-linked (GlcNAc...) Potential
	Disulfide bond	28 ↔ 37		By similarity
	Disulfide bond	31 ↔ 43		By similarity
	Disulfide bond	45 ↔ 65		By similarity
	Disulfide bond	71 ↔ 85		By similarity
	Disulfide bond	79 ↔ 94		By similarity
	Disulfide bond	96 ↔ 117		By similarity
Natural variations
	Alternative sequence	67 – 652	586	Missing in isoform 3.		VSP_009418
	Alternative sequence	67 – 118	52	Missing in isoform 2.		VSP_009417
	Natural variant	127	1	E → Q. Ref.2 Corresponds to variant rs4606855 [ dbSNP | Ensembl ].		VAR_024472
	Natural variant	236	1	A → V. Corresponds to variant rs34226397 [ dbSNP | Ensembl ].		VAR_055926
	Natural variant	385	1	R → Q. Ref.1 Ref.2 Corresponds to variant rs8102646 [ dbSNP | Ensembl ].		VAR_060442

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 60DCD73BB0DBEB74	FASTA	652	72,621
	10 20 30 40 50 60 MQGPLLLPGL CFLLSLFGAV TQKTKTSCAK CPPNASCVNN THCTCNHGYT SGSGQKLFTF 70 80 90 100 110 120 PLETCNDINE CTPPYSVYCG FNAVCYNVEG SFYCQCVPGY RLHSGNEQFS NSNENTCQDT 130 140 150 160 170 180 TSSKTTEGRK ELQKIVDKFE SLLTNQTLWR TEGRQEISST ATTILRDVES KVLETALKDP 190 200 210 220 230 240 EQKVLKIQND SVAIETQAIT DNCSEERKTF NLNVQMNSMD IRCSDIIQGD TQGPSAIAFI 250 260 270 280 290 300 SYSSLGNIIN ATFFEEMDKK DQVYLNSQVV SAAIGPKRNV SLSKSVTLTF QHVKMTPSTK 310 320 330 340 350 360 KVFCVYWKST GQGSQWSRDG CFLIHVNKSH TMCNCSHLSS FAVLMALTSQ EEDPVLTVIT 370 380 390 400 410 420 YVGLSVSLLC LLLAALTFLL CKAIRNTSTS LHLQLSLCLF LAHLLFLVGI DRTEPKVLCS 430 440 450 460 470 480 IIAGALHYLY LAAFTWMLLE GVHLFLTARN LTVVNYSSIN RLMKWIMFPV GYGVPAVTVA 490 500 510 520 530 540 ISAASWPHLY GTADRCWLHL DQGFMWSFLG PVCAIFSANL VLFILVFWIL KRKLSSLNSE 550 560 570 580 590 600 VSTIQNTRML AFKATAQLFI LGCTWCLGLL QVGPAAQVMA YLFTIINSLQ GFFIFLVYCL 610 620 630 640 650 LSQQVQKQYQ KWFREIVKSK SESETYTLSS KMGPDSKPSE GDVFPGQVKR KY
	Isoform 2 [UniParc]. Checksum: 44594300E1B4F0A5	FASTA	600	66,786
	10 20 30 40 50 60 MQGPLLLPGL CFLLSLFGAV TQKTKTSCAK CPPNASCVNN THCTCNHGYT SGSGQKLFTF 70 80 90 100 110 120 PLETCNDTTS SKTTEGRKEL QKIVDKFESL LTNQTLWRTE GRQEISSTAT TILRDVESKV 130 140 150 160 170 180 LETALKDPEQ KVLKIQNDSV AIETQAITDN CSEERKTFNL NVQMNSMDIR CSDIIQGDTQ 190 200 210 220 230 240 GPSAIAFISY SSLGNIINAT FFEEMDKKDQ VYLNSQVVSA AIGPKRNVSL SKSVTLTFQH 250 260 270 280 290 300 VKMTPSTKKV FCVYWKSTGQ GSQWSRDGCF LIHVNKSHTM CNCSHLSSFA VLMALTSQEE 310 320 330 340 350 360 DPVLTVITYV GLSVSLLCLL LAALTFLLCK AIRNTSTSLH LQLSLCLFLA HLLFLVGIDR 370 380 390 400 410 420 TEPKVLCSII AGALHYLYLA AFTWMLLEGV HLFLTARNLT VVNYSSINRL MKWIMFPVGY 430 440 450 460 470 480 GVPAVTVAIS AASWPHLYGT ADRCWLHLDQ GFMWSFLGPV CAIFSANLVL FILVFWILKR 490 500 510 520 530 540 KLSSLNSEVS TIQNTRMLAF KATAQLFILG CTWCLGLLQV GPAAQVMAYL FTIINSLQGF 550 560 570 580 590 600 FIFLVYCLLS QQVQKQYQKW FREIVKSKSE SETYTLSSKM GPDSKPSEGD VFPGQVKRKY
	Isoform 3 [UniParc]. Checksum: 0351F055DA4FD7C7	FASTA	66	6,996
	10 20 30 40 50 60 MQGPLLLPGL CFLLSLFGAV TQKTKTSCAK CPPNASCVNN THCTCNHGYT SGSGQKLFTF PLETCN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 is a new member of the EGF-TM7 family that recognizes a ligand on human macrophages and activated neutrophils." Stacey M., Lin H.-H., Hilyard K.L., Gordon S., McKnight A.J. J. Biol. Chem. 276:18863-18870(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT GLN-385.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS GLN-127 AND GLN-385.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF239764 mRNA. Translation: AAK15076.1. AY358817 mRNA. Translation: AAQ89176.1. AC022149 Genomic DNA. No translation available. AC090427 Genomic DNA. No translation available. AC135052 Genomic DNA. No translation available.
IPI	IPI00398872. IPI00641365. IPI00978675.
RefSeq	NP_115960.2. NM_032571.3.
UniGene	Hs.658712.
3D structure databases
ProteinModelPortal	Q9BY15.
SMR	Q9BY15. Positions 28-344.
ModBase	Search...
Protein family/group databases
MEROPS	S63.003.
GPCRDB	Search...
PTM databases
PhosphoSite	Q9BY15.
Polymorphism databases
DMDM	296434492.
Proteomic databases
PaxDb	Q9BY15.
PRIDE	Q9BY15.
Protocols and materials databases
DNASU	84658.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000253673; ENSP00000253673; ENSG00000131355. ENST00000344373; ENSP00000340758; ENSG00000131355.
GeneID	84658.
KEGG	hsa:84658.
UCSC	uc002mzi.4. human. uc010dzp.3. human.
Organism-specific databases
CTD	84658.
GeneCards	GC19M014730.
H-InvDB	HIX0022599.
HGNC	HGNC:23647. EMR3.
HPA	HPA015638.
MIM	606101. gene.
neXtProt	NX_Q9BY15.
PharmGKB	PA134956879.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG239437.
HOGENOM	HOG000294115.
HOVERGEN	HBG048917.
InParanoid	Q9BY15.
KO	K08444.
OMA	PLETCND.
PhylomeDB	Q9BY15.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q9BY15.
Bgee	Q9BY15.
CleanEx	HS_EMR3.
Genevestigator	Q9BY15.
GermOnline	ENSG00000131355. Homo sapiens.
Family and domain databases
InterPro	IPR000742. EG-like_dom. IPR001881. EGF-like_Ca-bd. IPR000152. EGF-type_Asp/Asn_hydroxyl_site. IPR018097. EGF_Ca-bd_CS. IPR017981. GPCR_2-like. IPR001740. GPCR_2_EMR1_rcpt. IPR000832. GPCR_2_secretin-like. IPR017983. GPCR_2_secretin-like_CS. IPR000203. GPS_dom. [Graphical view]
Pfam	PF00002. 7tm_2. 1 hit. PF07645. EGF_CA. 1 hit. PF01825. GPS. 1 hit. [Graphical view]
PRINTS	PR01128. EMR1HORMONER. PR00249. GPCRSECRETIN.
SMART	SM00179. EGF_CA. 1 hit. SM00303. GPS. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS50026. EGF_3. 1 hit. PS01187. EGF_CA. 1 hit. PS00650. G_PROTEIN_RECEP_F2_2. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. PS50221. GPS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	84658.
NextBio	74625.
SOURCE	Search...

Entry information

Entry name

EMR3_HUMAN

Accession

Primary (citable) accession number: Q9BY15

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.