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Protein

Adhesion G protein-coupled receptor E3

Gene

ADGRE3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Orphan receptor that may play a role myeloid-myeloid interactions during immune and inflammatory responses. A ligand for the soluble form of this receptor is present at the surface of monocytes-derived macrophages and activated neutrophils.1 Publication

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • G-protein coupled receptor activity Source: GDB

GO - Biological processi

  • cell surface receptor signaling pathway Source: InterPro
  • G-protein coupled receptor signaling pathway Source: GDB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131355-MONOMER.
ReactomeiR-HSA-373080. Class B/2 (Secretin family receptors).
R-HSA-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiP02.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor E31 Publication
Alternative name(s):
EGF-like module receptor 3
EGF-like module-containing mucin-like hormone receptor-like 3
Gene namesi
Name:ADGRE3Imported
Synonyms:EMR3
ORF Names:UNQ683/PRO1562
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:23647. ADGRE3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 357ExtracellularSequence analysisAdd BLAST336
Transmembranei358 – 378Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini379 – 389CytoplasmicSequence analysisAdd BLAST11
Transmembranei390 – 410Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini411 – 416ExtracellularSequence analysis6
Transmembranei417 – 437Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini438 – 464CytoplasmicSequence analysisAdd BLAST27
Transmembranei465 – 485Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini486 – 508ExtracellularSequence analysisAdd BLAST23
Transmembranei509 – 529Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini530 – 557CytoplasmicSequence analysisAdd BLAST28
Transmembranei558 – 578Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini579 – 580ExtracellularSequence analysis2
Transmembranei581 – 601Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini602 – 652CytoplasmicSequence analysisAdd BLAST51

GO - Cellular componenti

  • extracellular region Source: UniProtKB-SubCell
  • integral component of membrane Source: GDB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000131355.
PharmGKBiPA134956879.

Polymorphism and mutation databases

BioMutaiEMR3.
DMDMi296434492.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001287622 – 652Adhesion G protein-coupled receptor E3Add BLAST631

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 37PROSITE-ProRule annotation
Disulfide bondi31 ↔ 43PROSITE-ProRule annotation
Glycosylationi34N-linked (GlcNAc...)Sequence analysis1
Glycosylationi39N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi45 ↔ 65PROSITE-ProRule annotation
Disulfide bondi71 ↔ 85PROSITE-ProRule annotation
Disulfide bondi79 ↔ 94PROSITE-ProRule annotation
Disulfide bondi96 ↔ 117PROSITE-ProRule annotation
Glycosylationi145N-linked (GlcNAc...)Sequence analysis1
Glycosylationi189N-linked (GlcNAc...)Sequence analysis1
Glycosylationi202N-linked (GlcNAc...)Sequence analysis1
Glycosylationi250N-linked (GlcNAc...)Sequence analysis1
Glycosylationi279N-linked (GlcNAc...)Sequence analysis1
Glycosylationi327N-linked (GlcNAc...)Sequence analysis1
Glycosylationi334N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei338 – 339CleavageBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9BY15.
PeptideAtlasiQ9BY15.
PRIDEiQ9BY15.

PTM databases

iPTMnetiQ9BY15.
PhosphoSitePlusiQ9BY15.

Expressioni

Tissue specificityi

Displays a predominantly leukocyte-restricted expression, with highest levels in neutrophils, monocytes and macrophages.1 Publication

Gene expression databases

BgeeiENSG00000131355.
CleanExiHS_EMR3.
ExpressionAtlasiQ9BY15. baseline and differential.
GenevisibleiQ9BY15. HS.

Organism-specific databases

HPAiHPA015638.

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit).By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000253673.

Structurei

3D structure databases

ProteinModelPortaliQ9BY15.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 66EGF-like 1PROSITE-ProRule annotationAdd BLAST43
Domaini67 – 118EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST52
Domaini301 – 350GPSPROSITE-ProRule annotationAdd BLAST50

Sequence similaritiesi

Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ9BY15.
KOiK08444.
OMAiASCVNNT.
OrthoDBiEOG091G0387.
PhylomeDBiQ9BY15.
TreeFamiTF316380.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR009030. Growth_fac_rcpt_.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 1 hit.
PF16489. GAIN. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BY15-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQGPLLLPGL CFLLSLFGAV TQKTKTSCAK CPPNASCVNN THCTCNHGYT
60 70 80 90 100
SGSGQKLFTF PLETCNDINE CTPPYSVYCG FNAVCYNVEG SFYCQCVPGY
110 120 130 140 150
RLHSGNEQFS NSNENTCQDT TSSKTTEGRK ELQKIVDKFE SLLTNQTLWR
160 170 180 190 200
TEGRQEISST ATTILRDVES KVLETALKDP EQKVLKIQND SVAIETQAIT
210 220 230 240 250
DNCSEERKTF NLNVQMNSMD IRCSDIIQGD TQGPSAIAFI SYSSLGNIIN
260 270 280 290 300
ATFFEEMDKK DQVYLNSQVV SAAIGPKRNV SLSKSVTLTF QHVKMTPSTK
310 320 330 340 350
KVFCVYWKST GQGSQWSRDG CFLIHVNKSH TMCNCSHLSS FAVLMALTSQ
360 370 380 390 400
EEDPVLTVIT YVGLSVSLLC LLLAALTFLL CKAIRNTSTS LHLQLSLCLF
410 420 430 440 450
LAHLLFLVGI DRTEPKVLCS IIAGALHYLY LAAFTWMLLE GVHLFLTARN
460 470 480 490 500
LTVVNYSSIN RLMKWIMFPV GYGVPAVTVA ISAASWPHLY GTADRCWLHL
510 520 530 540 550
DQGFMWSFLG PVCAIFSANL VLFILVFWIL KRKLSSLNSE VSTIQNTRML
560 570 580 590 600
AFKATAQLFI LGCTWCLGLL QVGPAAQVMA YLFTIINSLQ GFFIFLVYCL
610 620 630 640 650
LSQQVQKQYQ KWFREIVKSK SESETYTLSS KMGPDSKPSE GDVFPGQVKR

KY
Length:652
Mass (Da):72,621
Last modified:May 18, 2010 - v2
Checksum:i60DCD73BB0DBEB74
GO
Isoform 2 (identifier: Q9BY15-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-118: Missing.

Show »
Length:600
Mass (Da):66,786
Checksum:i44594300E1B4F0A5
GO
Isoform 3 (identifier: Q9BY15-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-652: Missing.

Note: No experimental experiment available. Due to a 40-nucleotide deletion (nucleotides 439-479) resulting in a frameshift leading to a premature stop codon and the production of a truncated soluble form.Curated
Show »
Length:117
Mass (Da):12,703
Checksum:i62A00A651534FADC
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_024472127E → Q.1 PublicationCorresponds to variant rs4606855dbSNPEnsembl.1
Natural variantiVAR_055926236A → V.Corresponds to variant rs34226397dbSNPEnsembl.1
Natural variantiVAR_060442385R → Q.2 PublicationsCorresponds to variant rs8102646dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00941767 – 118Missing in isoform 2. 1 PublicationAdd BLAST52
Alternative sequenceiVSP_009418118 – 652Missing in isoform 3. CuratedAdd BLAST535

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239764 mRNA. Translation: AAK15076.1.
AY358817 mRNA. Translation: AAQ89176.1.
AC022149 Genomic DNA. No translation available.
AC090427 Genomic DNA. No translation available.
AC135052 Genomic DNA. No translation available.
CCDSiCCDS12315.1. [Q9BY15-1]
CCDS74297.1. [Q9BY15-2]
RefSeqiNP_001276087.1. NM_001289158.1. [Q9BY15-2]
NP_001276088.1. NM_001289159.1.
NP_115960.2. NM_032571.4. [Q9BY15-1]
UniGeneiHs.658712.

Genome annotation databases

EnsembliENST00000253673; ENSP00000253673; ENSG00000131355. [Q9BY15-1]
ENST00000344373; ENSP00000340758; ENSG00000131355. [Q9BY15-2]
GeneIDi84658.
KEGGihsa:84658.
UCSCiuc002mzi.6. human. [Q9BY15-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239764 mRNA. Translation: AAK15076.1.
AY358817 mRNA. Translation: AAQ89176.1.
AC022149 Genomic DNA. No translation available.
AC090427 Genomic DNA. No translation available.
AC135052 Genomic DNA. No translation available.
CCDSiCCDS12315.1. [Q9BY15-1]
CCDS74297.1. [Q9BY15-2]
RefSeqiNP_001276087.1. NM_001289158.1. [Q9BY15-2]
NP_001276088.1. NM_001289159.1.
NP_115960.2. NM_032571.4. [Q9BY15-1]
UniGeneiHs.658712.

3D structure databases

ProteinModelPortaliQ9BY15.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000253673.

Protein family/group databases

MEROPSiP02.003.
GPCRDBiSearch...

PTM databases

iPTMnetiQ9BY15.
PhosphoSitePlusiQ9BY15.

Polymorphism and mutation databases

BioMutaiEMR3.
DMDMi296434492.

Proteomic databases

PaxDbiQ9BY15.
PeptideAtlasiQ9BY15.
PRIDEiQ9BY15.

Protocols and materials databases

DNASUi84658.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253673; ENSP00000253673; ENSG00000131355. [Q9BY15-1]
ENST00000344373; ENSP00000340758; ENSG00000131355. [Q9BY15-2]
GeneIDi84658.
KEGGihsa:84658.
UCSCiuc002mzi.6. human. [Q9BY15-1]

Organism-specific databases

CTDi84658.
GeneCardsiADGRE3.
H-InvDBHIX0022599.
HGNCiHGNC:23647. ADGRE3.
HPAiHPA015638.
MIMi606101. gene.
neXtProtiNX_Q9BY15.
OpenTargetsiENSG00000131355.
PharmGKBiPA134956879.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ9BY15.
KOiK08444.
OMAiASCVNNT.
OrthoDBiEOG091G0387.
PhylomeDBiQ9BY15.
TreeFamiTF316380.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000131355-MONOMER.
ReactomeiR-HSA-373080. Class B/2 (Secretin family receptors).
R-HSA-6798695. Neutrophil degranulation.

Miscellaneous databases

GeneWikiiEMR3.
GenomeRNAii84658.
PROiQ9BY15.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000131355.
CleanExiHS_EMR3.
ExpressionAtlasiQ9BY15. baseline and differential.
GenevisibleiQ9BY15. HS.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR009030. Growth_fac_rcpt_.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 1 hit.
PF16489. GAIN. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGRE3_HUMAN
AccessioniPrimary (citable) accession number: Q9BY15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Has no murine ortholog.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.