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Protein

EGF-like module-containing mucin-like hormone receptor-like 3

Gene

EMR3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor probably involved in myeloid interactions during immune and inflammatory responses. A ligand for the soluble form of this receptor is present at the surface of momocytes-derived macrophages and activated neutrophils.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei338 – 3392CleavageBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. G-protein coupled receptor activity Source: GDB

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: GDB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_18372. Class B/2 (Secretin family receptors).

Protein family/group databases

MEROPSiS63.003.

Names & Taxonomyi

Protein namesi
Recommended name:
EGF-like module-containing mucin-like hormone receptor-like 3
Alternative name(s):
EGF-like module receptor 3
Cleaved into the following 2 chains:
Gene namesi
Name:EMR3
ORF Names:UNQ683/PRO1562
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:23647. EMR3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 357336ExtracellularSequence AnalysisAdd
BLAST
Transmembranei358 – 37821Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini379 – 38911CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei390 – 41021Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini411 – 4166ExtracellularSequence Analysis
Transmembranei417 – 43721Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini438 – 46427CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei465 – 48521Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini486 – 50823ExtracellularSequence AnalysisAdd
BLAST
Transmembranei509 – 52921Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini530 – 55728CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei558 – 57821Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini579 – 5802ExtracellularSequence Analysis
Transmembranei581 – 60121Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini602 – 65251CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
  2. integral component of membrane Source: GDB
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134956879.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 652631EGF-like module-containing mucin-like hormone receptor-like 3PRO_0000012876Add
BLAST
Chaini22 – 338317EGF-like module-containing mucin-like hormone receptor-like 3 subunit alphaBy similarityPRO_0000296239Add
BLAST
Chaini339 – 652314EGF-like module-containing mucin-like hormone receptor-like 3 subunit betaBy similarityPRO_0000296240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi28 ↔ 37PROSITE-ProRule annotation
Disulfide bondi31 ↔ 43PROSITE-ProRule annotation
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi45 ↔ 65PROSITE-ProRule annotation
Disulfide bondi71 ↔ 85PROSITE-ProRule annotation
Disulfide bondi79 ↔ 94PROSITE-ProRule annotation
Disulfide bondi96 ↔ 117PROSITE-ProRule annotation
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9BY15.
PRIDEiQ9BY15.

PTM databases

PhosphoSiteiQ9BY15.

Expressioni

Tissue specificityi

Displays a predominantly leukocyte-restricted expression, with highest levels in neutrophils, monocytes and macrophages.

Gene expression databases

BgeeiQ9BY15.
CleanExiHS_EMR3.
ExpressionAtlasiQ9BY15. baseline and differential.
GenevestigatoriQ9BY15.

Organism-specific databases

HPAiHPA015638.

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9BY15.
SMRiQ9BY15. Positions 28-344, 456-602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 6643EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini67 – 11852EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini301 – 35050GPSPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239437.
GeneTreeiENSGT00760000118787.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ9BY15.
KOiK08444.
OMAiWSRDGCF.
OrthoDBiEOG78H3SM.
PhylomeDBiQ9BY15.
TreeFamiTF316380.

Family and domain databases

InterProiIPR022624. DUF3497.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR009030. Growth_fac_rcpt_N_dom.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF12003. DUF3497. 1 hit.
PF07645. EGF_CA. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BY15-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQGPLLLPGL CFLLSLFGAV TQKTKTSCAK CPPNASCVNN THCTCNHGYT
60 70 80 90 100
SGSGQKLFTF PLETCNDINE CTPPYSVYCG FNAVCYNVEG SFYCQCVPGY
110 120 130 140 150
RLHSGNEQFS NSNENTCQDT TSSKTTEGRK ELQKIVDKFE SLLTNQTLWR
160 170 180 190 200
TEGRQEISST ATTILRDVES KVLETALKDP EQKVLKIQND SVAIETQAIT
210 220 230 240 250
DNCSEERKTF NLNVQMNSMD IRCSDIIQGD TQGPSAIAFI SYSSLGNIIN
260 270 280 290 300
ATFFEEMDKK DQVYLNSQVV SAAIGPKRNV SLSKSVTLTF QHVKMTPSTK
310 320 330 340 350
KVFCVYWKST GQGSQWSRDG CFLIHVNKSH TMCNCSHLSS FAVLMALTSQ
360 370 380 390 400
EEDPVLTVIT YVGLSVSLLC LLLAALTFLL CKAIRNTSTS LHLQLSLCLF
410 420 430 440 450
LAHLLFLVGI DRTEPKVLCS IIAGALHYLY LAAFTWMLLE GVHLFLTARN
460 470 480 490 500
LTVVNYSSIN RLMKWIMFPV GYGVPAVTVA ISAASWPHLY GTADRCWLHL
510 520 530 540 550
DQGFMWSFLG PVCAIFSANL VLFILVFWIL KRKLSSLNSE VSTIQNTRML
560 570 580 590 600
AFKATAQLFI LGCTWCLGLL QVGPAAQVMA YLFTIINSLQ GFFIFLVYCL
610 620 630 640 650
LSQQVQKQYQ KWFREIVKSK SESETYTLSS KMGPDSKPSE GDVFPGQVKR

KY
Length:652
Mass (Da):72,621
Last modified:May 18, 2010 - v2
Checksum:i60DCD73BB0DBEB74
GO
Isoform 2 (identifier: Q9BY15-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-118: Missing.

Show »
Length:600
Mass (Da):66,786
Checksum:i44594300E1B4F0A5
GO
Isoform 3 (identifier: Q9BY15-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-652: Missing.

Note: Soluble form.

Show »
Length:66
Mass (Da):6,996
Checksum:i0351F055DA4FD7C7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti127 – 1271E → Q.1 Publication
Corresponds to variant rs4606855 [ dbSNP | Ensembl ].
VAR_024472
Natural varianti236 – 2361A → V.
Corresponds to variant rs34226397 [ dbSNP | Ensembl ].
VAR_055926
Natural varianti385 – 3851R → Q.2 Publications
Corresponds to variant rs8102646 [ dbSNP | Ensembl ].
VAR_060442

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei67 – 652586Missing in isoform 3. CuratedVSP_009418Add
BLAST
Alternative sequencei67 – 11852Missing in isoform 2. 2 PublicationsVSP_009417Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239764 mRNA. Translation: AAK15076.1.
AY358817 mRNA. Translation: AAQ89176.1.
AC022149 Genomic DNA. No translation available.
AC090427 Genomic DNA. No translation available.
AC135052 Genomic DNA. No translation available.
CCDSiCCDS12315.1. [Q9BY15-1]
CCDS74297.1. [Q9BY15-2]
RefSeqiNP_001276087.1. NM_001289158.1. [Q9BY15-2]
NP_001276088.1. NM_001289159.1.
NP_115960.2. NM_032571.4. [Q9BY15-1]
UniGeneiHs.658712.

Genome annotation databases

EnsembliENST00000253673; ENSP00000253673; ENSG00000131355. [Q9BY15-1]
ENST00000344373; ENSP00000340758; ENSG00000131355. [Q9BY15-2]
GeneIDi84658.
KEGGihsa:84658.
UCSCiuc002mzi.4. human. [Q9BY15-1]
uc010dzp.3. human. [Q9BY15-2]

Polymorphism databases

DMDMi296434492.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239764 mRNA. Translation: AAK15076.1.
AY358817 mRNA. Translation: AAQ89176.1.
AC022149 Genomic DNA. No translation available.
AC090427 Genomic DNA. No translation available.
AC135052 Genomic DNA. No translation available.
CCDSiCCDS12315.1. [Q9BY15-1]
CCDS74297.1. [Q9BY15-2]
RefSeqiNP_001276087.1. NM_001289158.1. [Q9BY15-2]
NP_001276088.1. NM_001289159.1.
NP_115960.2. NM_032571.4. [Q9BY15-1]
UniGeneiHs.658712.

3D structure databases

ProteinModelPortaliQ9BY15.
SMRiQ9BY15. Positions 28-344, 456-602.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS63.003.
GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9BY15.

Polymorphism databases

DMDMi296434492.

Proteomic databases

PaxDbiQ9BY15.
PRIDEiQ9BY15.

Protocols and materials databases

DNASUi84658.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000253673; ENSP00000253673; ENSG00000131355. [Q9BY15-1]
ENST00000344373; ENSP00000340758; ENSG00000131355. [Q9BY15-2]
GeneIDi84658.
KEGGihsa:84658.
UCSCiuc002mzi.4. human. [Q9BY15-1]
uc010dzp.3. human. [Q9BY15-2]

Organism-specific databases

CTDi84658.
GeneCardsiGC19M014730.
H-InvDBHIX0022599.
HGNCiHGNC:23647. EMR3.
HPAiHPA015638.
MIMi606101. gene.
neXtProtiNX_Q9BY15.
PharmGKBiPA134956879.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG239437.
GeneTreeiENSGT00760000118787.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ9BY15.
KOiK08444.
OMAiWSRDGCF.
OrthoDBiEOG78H3SM.
PhylomeDBiQ9BY15.
TreeFamiTF316380.

Enzyme and pathway databases

ReactomeiREACT_18372. Class B/2 (Secretin family receptors).

Miscellaneous databases

GeneWikiiEMR3.
GenomeRNAii84658.
NextBioi74625.
PROiQ9BY15.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BY15.
CleanExiHS_EMR3.
ExpressionAtlasiQ9BY15. baseline and differential.
GenevestigatoriQ9BY15.

Family and domain databases

InterProiIPR022624. DUF3497.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
IPR009030. Growth_fac_rcpt_N_dom.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF12003. DUF3497. 1 hit.
PF07645. EGF_CA. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 is a new member of the EGF-TM7 family that recognizes a ligand on human macrophages and activated neutrophils."
    Stacey M., Lin H.-H., Hilyard K.L., Gordon S., McKnight A.J.
    J. Biol. Chem. 276:18863-18870(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), VARIANT GLN-385.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS GLN-127 AND GLN-385.
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiEMR3_HUMAN
AccessioniPrimary (citable) accession number: Q9BY15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: May 18, 2010
Last modified: March 4, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.