ID   EBPL_HUMAN              Reviewed;         206 AA.
AC   Q9BY08; A6NJ59; Q569H7; Q5JVN2; Q5JVN3; Q5JVN4; Q5JVN5; Q5JVN6;
DT   18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   27-MAR-2024, entry version 151.
DE   RecName: Full=Emopamil-binding protein-like;
DE   AltName: Full=Emopamil-binding-related protein;
GN   Name=EBPL; Synonyms=EBRP, ERP;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, SUBCELLULAR
RP   LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Liver;
RX   PubMed=12760743; DOI=10.1042/bj20030465;
RA   Moebius F.F., Fitzky B.U., Wietzorrek G., Haidekker A., Eder A.,
RA   Glossmann H.;
RT   "Cloning of an emopamil-binding protein (EBP)-like protein that lacks
RT   sterol delta8-delta7 isomerase activity.";
RL   Biochem. J. 374:229-237(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15057823; DOI=10.1038/nature02379;
RA   Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA   Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S.,
RA   Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P.,
RA   Ambrose K.D., Andrews D.T., Ashwell R.I.S., Babbage A.K., Bagguley C.L.,
RA   Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P.,
RA   Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA   Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C.,
RA   Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P.,
RA   Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L.,
RA   Frankish A.G., Frankland J., French L., Garner P., Garnett J.,
RA   Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M.,
RA   Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D.,
RA   Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D.,
RA   Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J.,
RA   Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA   Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S.,
RA   Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R.,
RA   Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W.,
RA   Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P.,
RA   Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L.,
RA   Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R.,
RA   Rogers J., Ross M.T.;
RT   "The DNA sequence and analysis of human chromosome 13.";
RL   Nature 428:522-528(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Brain, and Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Does not possess sterol isomerase activity and does not bind
CC       sigma ligands. {ECO:0000269|PubMed:12760743}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12760743}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:12760743}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:12760743}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=5;
CC       Name=1;
CC         IsoId=Q9BY08-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9BY08-2; Sequence=VSP_035418, VSP_035425;
CC       Name=3;
CC         IsoId=Q9BY08-3; Sequence=VSP_035417, VSP_035422;
CC       Name=4;
CC         IsoId=Q9BY08-4; Sequence=VSP_035419, VSP_035421;
CC       Name=5;
CC         IsoId=Q9BY08-5; Sequence=VSP_035420, VSP_035423, VSP_035424;
CC   -!- TISSUE SPECIFICITY: Widely expressed with highest levels in liver, lung
CC       and kidney. {ECO:0000269|PubMed:12760743}.
CC   -!- SIMILARITY: Belongs to the EBP family. {ECO:0000305}.
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DR   EMBL; AF243433; AAK28348.1; -; mRNA.
DR   EMBL; AL135901; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471075; EAX08832.1; -; Genomic_DNA.
DR   EMBL; BC018478; AAH18478.1; -; mRNA.
DR   EMBL; BC092471; AAH92471.1; -; mRNA.
DR   CCDS; CCDS9420.1; -. [Q9BY08-1]
DR   RefSeq; NP_115954.1; NM_032565.4. [Q9BY08-1]
DR   AlphaFoldDB; Q9BY08; -.
DR   SMR; Q9BY08; -.
DR   BioGRID; 124173; 3.
DR   IntAct; Q9BY08; 3.
DR   STRING; 9606.ENSP00000242827; -.
DR   BindingDB; Q9BY08; -.
DR   ChEMBL; CHEMBL2311238; -.
DR   DrugCentral; Q9BY08; -.
DR   BioMuta; EBPL; -.
DR   DMDM; 18202741; -.
DR   jPOST; Q9BY08; -.
DR   MassIVE; Q9BY08; -.
DR   PaxDb; 9606-ENSP00000242827; -.
DR   PeptideAtlas; Q9BY08; -.
DR   ProteomicsDB; 79554; -. [Q9BY08-1]
DR   ProteomicsDB; 79555; -. [Q9BY08-2]
DR   ProteomicsDB; 79556; -. [Q9BY08-3]
DR   ProteomicsDB; 79557; -. [Q9BY08-4]
DR   ProteomicsDB; 79558; -. [Q9BY08-5]
DR   TopDownProteomics; Q9BY08-1; -. [Q9BY08-1]
DR   Antibodypedia; 53037; 80 antibodies from 13 providers.
DR   DNASU; 84650; -.
DR   Ensembl; ENST00000242827.11; ENSP00000242827.6; ENSG00000123179.14. [Q9BY08-1]
DR   Ensembl; ENST00000378268.1; ENSP00000367516.1; ENSG00000123179.14. [Q9BY08-2]
DR   Ensembl; ENST00000378270.5; ENSP00000367519.5; ENSG00000123179.14. [Q9BY08-3]
DR   Ensembl; ENST00000378272.9; ENSP00000367521.5; ENSG00000123179.14. [Q9BY08-4]
DR   GeneID; 84650; -.
DR   KEGG; hsa:84650; -.
DR   MANE-Select; ENST00000242827.11; ENSP00000242827.6; NM_032565.5; NP_115954.1.
DR   UCSC; uc001vdg.5; human. [Q9BY08-1]
DR   AGR; HGNC:18061; -.
DR   DisGeNET; 84650; -.
DR   GeneCards; EBPL; -.
DR   HGNC; HGNC:18061; EBPL.
DR   HPA; ENSG00000123179; Tissue enhanced (liver).
DR   MIM; 617335; gene.
DR   neXtProt; NX_Q9BY08; -.
DR   OpenTargets; ENSG00000123179; -.
DR   PharmGKB; PA134875632; -.
DR   VEuPathDB; HostDB:ENSG00000123179; -.
DR   eggNOG; KOG4826; Eukaryota.
DR   GeneTree; ENSGT00530000063715; -.
DR   HOGENOM; CLU_2346090_0_0_1; -.
DR   InParanoid; Q9BY08; -.
DR   OMA; VYLWLYL; -.
DR   OrthoDB; 4182271at2759; -.
DR   PhylomeDB; Q9BY08; -.
DR   TreeFam; TF314716; -.
DR   PathwayCommons; Q9BY08; -.
DR   SignaLink; Q9BY08; -.
DR   BioGRID-ORCS; 84650; 30 hits in 1155 CRISPR screens.
DR   ChiTaRS; EBPL; human.
DR   GenomeRNAi; 84650; -.
DR   Pharos; Q9BY08; Tchem.
DR   PRO; PR:Q9BY08; -.
DR   Proteomes; UP000005640; Chromosome 13.
DR   RNAct; Q9BY08; Protein.
DR   Bgee; ENSG00000123179; Expressed in right lobe of liver and 100 other cell types or tissues.
DR   ExpressionAtlas; Q9BY08; baseline and differential.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:HPA.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0047750; F:cholestenol delta-isomerase activity; IEA:InterPro.
DR   GO; GO:0016125; P:sterol metabolic process; IEA:InterPro.
DR   InterPro; IPR007905; EBP.
DR   InterPro; IPR033118; EXPERA.
DR   PANTHER; PTHR14207:SF4; EMOPAMIL-BINDING PROTEIN-LIKE; 1.
DR   PANTHER; PTHR14207; STEROL ISOMERASE; 1.
DR   Pfam; PF05241; EBP; 1.
DR   PROSITE; PS51751; EXPERA; 1.
DR   Genevisible; Q9BY08; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..206
FT                   /note="Emopamil-binding protein-like"
FT                   /id="PRO_0000174347"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          39..184
FT                   /note="EXPERA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01087"
FT   VAR_SEQ         58..102
FT                   /note="EGPFVYLSLVGNVANSDGLIASLWKEYGKADARWVYFDPTIVSVE -> AFP
FT                   ADHPVRVRAVWLLDDLPPRVAHQKPQPQHQQLAVLLALPVFF (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035417"
FT   VAR_SEQ         81..152
FT                   /note="WKEYGKADARWVYFDPTIVSVEILTVALDGSLALFLIYAIVKEKYYRHFLQI
FT                   TLCVCELYGCWMTFLPEWLT -> CESRSVPQAGAQWHHLGSLQAPPPRFTPFSCLSLP
FT                   SSWDYRRPPPRLANFFVFLVETGFHHVSQDGLDLLTS (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035418"
FT   VAR_SEQ         81..97
FT                   /note="WKEYGKADARWVYFDPT -> CISCRSPCACASCMAAG (in isoform
FT                   4)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035419"
FT   VAR_SEQ         81..87
FT                   /note="WKEYGKA -> S (in isoform 5)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035420"
FT   VAR_SEQ         98..206
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035421"
FT   VAR_SEQ         103..206
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035422"
FT   VAR_SEQ         128..134
FT                   /note="HFLQITL -> DRVAWGE (in isoform 5)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035423"
FT   VAR_SEQ         135..206
FT                   /note="Missing (in isoform 5)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035424"
FT   VAR_SEQ         153..206
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_035425"
FT   CONFLICT        31
FT                   /note="R -> H (in Ref. 4; AAH92471)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   206 AA;  23204 MW;  7093AC7E638B89D0 CRC64;
     MGAEWELGAE AGGSLLLCAA LLAAGCALGL RLGRGQGAAD RGALIWLCYD ALVHFALEGP
     FVYLSLVGNV ANSDGLIASL WKEYGKADAR WVYFDPTIVS VEILTVALDG SLALFLIYAI
     VKEKYYRHFL QITLCVCELY GCWMTFLPEW LTRSPNLNTS NWLYCWLYLF FFNGVWVLIP
     GLLLWQSWLE LKKMHQKETS SVKKFQ
//