Q9BY07 (S4A5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Electrogenic sodium bicarbonate cotransporter 4

Alternative name(s):
NBCe2
Solute carrier family 4 member 5

Gene names

Name:	SLC4A5
Synonyms:	NBC4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1137 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mediates sodium- and bicarbonate-dependent electrogenic sodium bicarbonate cotransport, with a Na+:HCO3- stoichiometry of 2:1. May have a housekeeping function in regulating the pH of tissues in which it is expressed. May play a role in mediating Na+:HCO3- cotransport in hepatocytes and intrahepatic cholangiocytes. Also may be important in protecting the renal paranchyma from alterations in urine pH. Ref.4 Ref.5 Ref.6 UniProtKB Q6RI88
Enzyme regulation	Activity is inhibited by 4,4'-di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS - an inhibitor of several anion channels and transporters).
Subcellular location	Apical cell membrane; Multi-pass membrane protein. Note: Expressed in the apical plasma membrane domain of a subset of collecting ducts in the renal medulla. Ref.10
Tissue specificity	Highest expression observed in liver, spleen and testis; moderate expression in the choroid plexus, hippocampus, cerebrum and cerebellum of brain, and in kidney cortex and kidney medulla. Also observed in heart, pancreas, muscle, lung, placenta, stomach and small intestine. Weakest expression seen in peripheral blood lymphocytes, colon, duodenum, jejunum, ileum and skeletal muscle. Ref.1 Ref.2 Ref.4 Ref.10
Sequence similarities	Belongs to the anion exchanger (TC 2.A.31) family. [View classification]
Sequence caution	The sequence AAG18492.1 differs from that shown. Reason: Frameshift at position 1047. The sequence AAL50802.1 differs from that shown. Reason: Frameshift at positions 760, 925 and 1047. The sequence EAW99691.1 differs from that shown. Reason: Frameshift at position 1047.

Ontologies

Keywords
Biological process	Anion exchange Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Molecular_function	inorganic anion exchanger activity Inferred from electronic annotation. Source: InterPro sodium:bicarbonate symporter activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.2 (identifier: Q9BY07-1)

Also known as: NBC4a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 Ref.2 Ref.7 (identifier: Q9BY07-2)

Also known as: NBC4b;

The sequence of this isoform differs from the canonical sequence as follows:
     1046-1046: M → MSLSTTD

Isoform 3 Ref.4 Ref.7 (identifier: Q9BY07-3)

Also known as: NBC4c;

The sequence of this isoform differs from the canonical sequence as follows:
     973-988: Missing.

Isoform 4 Ref.3 Ref.7 (identifier: Q9BY07-4)

Also known as: NBC4d;

The sequence of this isoform differs from the canonical sequence as follows:
     950-1046: Missing.

Isoform 5 Ref.9 (identifier: Q9BY07-5)

Also known as: isoform d variant;

The sequence of this isoform differs from the canonical sequence as follows:
     88-91: SRTR → EHKG
     866-993: KAAGYHLDLF...KKLTPFWERC → GTESNRHHRL...HLFPARPGLD
     994-1137: Missing.

Note: No experimental confirmation available. Incomplete sequence.

Isoform 6 Ref.6 (identifier: Q9BY07-6)

Also known as: NBC4f;

The sequence of this isoform differs from the canonical sequence as follows:
     774-811: Missing.
     866-924: Missing.
     973-988: Missing.
     1046-1046: M → MSLSTTD
     1071-1075: NILPE → KIEFP
     1076-1137: Missing.

Isoform 7 Ref.8 (identifier: Q9BY07-7)

The sequence of this isoform differs from the canonical sequence as follows:
     27-91: ECPPIHIGLPVPTYPQRKTDQKGHLSGLQKVHWGLRPDQPQQELTGPGSGASSQDSSMDLISRTR → G
     774-811: Missing.
     973-988: Missing.

Note: No experimental confirmation available.

Isoform 8 Ref.6 (identifier: Q9BY07-8)

Also known as: NBC4e;

The sequence of this isoform differs from the canonical sequence as follows:
     1068-1137: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1137	1137	Electrogenic sodium bicarbonate cotransporter 4		PRO_0000328920
Regions
	Topological domain	1 – 523	523	Cytoplasmic Potential
	Transmembrane	524 – 544	21	Helical; Potential
	Topological domain	545 – 566	22	Extracellular Potential
	Transmembrane	567 – 587	21	Helical; Potential
	Topological domain	588 – 608	21	Cytoplasmic Potential
	Transmembrane	609 – 629	21	Helical; Potential
	Topological domain	630 – 639	10	Extracellular Potential
	Transmembrane	640 – 660	21	Helical; Potential
	Topological domain	661 – 738	78	Cytoplasmic Potential
	Transmembrane	739 – 759	21	Helical; Potential
	Topological domain	760 – 776	17	Extracellular Potential
	Transmembrane	777 – 797	21	Helical; Potential
	Topological domain	798 – 827	30	Cytoplasmic Potential
	Transmembrane	828 – 848	21	Helical; Potential
	Topological domain	849 – 873	25	Extracellular Potential
	Transmembrane	874 – 894	21	Helical; Potential
	Topological domain	895 – 930	36	Cytoplasmic Potential
	Transmembrane	931 – 951	21	Helical; Potential
	Topological domain	952	1	Extracellular Potential
	Transmembrane	953 – 973	21	Helical; Potential
	Topological domain	974 – 1011	38	Cytoplasmic Potential
	Transmembrane	1012 – 1032	21	Helical; Potential
	Transmembrane	1033 – 1053	21	Helical; Potential
	Topological domain	1054 – 1137	84	Extracellular Potential
	Compositional bias	433 – 497	65	Gly-rich
Natural variations
	Alternative sequence	27 – 91	65	ECPPI…ISRTR → G in isoform 7. Ref.8		VSP_052770
	Alternative sequence	88 – 91	4	SRTR → EHKG in isoform 5. Ref.9		VSP_052771
	Alternative sequence	774 – 811	38	Missing in isoform 6 and isoform 7. Ref.6 Ref.8		VSP_052772
	Alternative sequence	866 – 993	128	KAAGY…FWERC → GTESNRHHRLHPDGNLCLPG SHPKVYPPAGAVRSLPLHGR GLPEWHPVLGTLQALPDASQ APAGPCLPAARAAAPDPPLH PGADPLPGGALDPQIHGGCH HLPGHDPGPHHRSKASGFHL FPARPGLD in isoform 5. Ref.9		VSP_052773
	Alternative sequence	866 – 924	59	Missing in isoform 6. Ref.6		VSP_052774
	Alternative sequence	950 – 1046	97	Missing in isoform 4. Ref.3		VSP_052775
	Alternative sequence	973 – 988	16	Missing in isoform 3, isoform 6 and isoform 7. Ref.5 Ref.6 Ref.8		VSP_052776
	Alternative sequence	994 – 1137	144	Missing in isoform 5. Ref.9		VSP_052777
	Alternative sequence	1046	1	M → MSLSTTD in isoform 2 and isoform 6. Ref.1 Ref.2 Ref.6		VSP_052778
	Alternative sequence	1068 – 1137	70	Missing in isoform 8. Ref.6		VSP_052779
	Alternative sequence	1071 – 1075	5	NILPE → KIEFP in isoform 6. Ref.6		VSP_052780
	Alternative sequence	1076 – 1137	62	Missing in isoform 6. Ref.6		VSP_052781
	Natural variant	251	1	S → N. Corresponds to variant rs17009792 [ dbSNP | Ensembl ].		VAR_048350
	Natural variant	253	1	H → Y. Ref.9 Corresponds to variant rs55651232 [ dbSNP | Ensembl ].		VAR_061032
Experimental info
	Sequence conflict	612	1	H → Y in AAL48291. Ref.6
	Sequence conflict	612	1	H → Y in AAL50802. Ref.6
	Sequence conflict	699	1	A → T in AAL48291. Ref.6
	Sequence conflict	759	1	L → R in AAL50802. Ref.6
	Sequence conflict	813	1	T → P in AAK26741. Ref.2
	Sequence conflict	896	1	T → M in AAL48291. Ref.6
	Sequence conflict	923	1	G → R in AAL48291. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (NBC4a) [UniParc]. Last modified April 8, 2008. Version 2. Checksum: ED4E4A4DC2CA17F1	FASTA	1,137	126,255
	10 20 30 40 50 60 MKVKEEKAGV GKLDHTNHRR RFPDQKECPP IHIGLPVPTY PQRKTDQKGH LSGLQKVHWG 70 80 90 100 110 120 LRPDQPQQEL TGPGSGASSQ DSSMDLISRT RSPAAEQLQD ILGEEDEAPN PTLFTEMDTL 130 140 150 160 170 180 QHDGDQMEWK ESARWIKFEE KVEEGGERWS KPHVSTLSLH SLFELRTCLQ TGTVLLDLDS 190 200 210 220 230 240 GSLPQIIDDV IEKQIEDGLL RPELRERVSY VLLRRHRHQT KKPIHRSLAD IGKSVSTTNR 250 260 270 280 290 300 SPARSPGAGP SLHHSTEDLR MRQSANYGRL CHAQSRSMND ISLTPNTDQR KNKFMKKIPK 310 320 330 340 350 360 DSEASNVLVG EVDFLDQPFI AFVRLIQSAM LGGVTEVPVP TRFLFILLGP SGRAKSYNEI 370 380 390 400 410 420 GRAIATLMVD DLFSDVAYKA RNREDLIAGI DEFLDEVIVL PPGEWDPNIR IEPPKKVPSA 430 440 450 460 470 480 DKRKSVFSLA ELGQMNGSVG GGGGAPGGGN GGGGGGGSGG GAGSGGAGGT SSGDDGEMPA 490 500 510 520 530 540 MHEIGEELIW TGRFFGGLCL DIKRKLPWFP SDFYDGFHIQ SISAILFIYL GCITNAITFG 550 560 570 580 590 600 GLLGDATDNY QGVMESFLGT AMAGSLFCLF SGQPLIILSS TGPILIFEKL LFDFSKGNGL 610 620 630 640 650 660 DYMEFRLWIG LHSAVQCLIL VATDASFIIK YITRFTEEGF STLISFIFIY DAIKKMIGAF 670 680 690 700 710 720 KYYPINMDFK PNFITTYKCE CVAPDTVNTT VFNASAPLAP DTNASLYNLL NLTALDWSLL 730 740 750 760 770 780 SKKECLSYGG RLLGNSCKFI PDLALMSFIL FFGTYSMTLT LKKFKFSRYF PTKVRALVAD 790 800 810 820 830 840 FSIVFSILMF CGIDACFGLE TPKLHVPSVI KPTRPDRGWF VAPFGKNPWW VYPASILPAL 850 860 870 880 890 900 LVTILIFMDQ QITAVIVNRK ENKLKKAAGY HLDLFWVGIL MALCSFMGLP WYVAATVISI 910 920 930 940 950 960 AHIDSLKMET ETSAPGEQPQ FLGVREQRVT GIIVFILTGI SVFLAPILKC IPLPVLYGVF 970 980 990 1000 1010 1020 LYMGVASLNG IQMGTGGSEF KIQKKLTPFW ERCKLFLMPA KHQPDHAFLR HVPLRRIHLF 1030 1040 1050 1060 1070 1080 TLVQILCLAV LWILKSTVAA IIFPVMILGL IIVRRLLDFI FSQHDLAWID NILPEKEKKE 1090 1100 1110 1120 1130 TDKKRKRKKG AHEDCDEEPQ FPPPSVIKIP MESVQSDPQN GIHCIARKRS SSWSYSL
	Isoform 2 (NBC4b) [UniParc]. Checksum: 3C32B411955873FD	FASTA	1,143	126,860
	10 20 30 40 50 60 MKVKEEKAGV GKLDHTNHRR RFPDQKECPP IHIGLPVPTY PQRKTDQKGH LSGLQKVHWG 70 80 90 100 110 120 LRPDQPQQEL TGPGSGASSQ DSSMDLISRT RSPAAEQLQD ILGEEDEAPN PTLFTEMDTL 130 140 150 160 170 180 QHDGDQMEWK ESARWIKFEE KVEEGGERWS KPHVSTLSLH SLFELRTCLQ TGTVLLDLDS 190 200 210 220 230 240 GSLPQIIDDV IEKQIEDGLL RPELRERVSY VLLRRHRHQT KKPIHRSLAD IGKSVSTTNR 250 260 270 280 290 300 SPARSPGAGP SLHHSTEDLR MRQSANYGRL CHAQSRSMND ISLTPNTDQR KNKFMKKIPK 310 320 330 340 350 360 DSEASNVLVG EVDFLDQPFI AFVRLIQSAM LGGVTEVPVP TRFLFILLGP SGRAKSYNEI 370 380 390 400 410 420 GRAIATLMVD DLFSDVAYKA RNREDLIAGI DEFLDEVIVL PPGEWDPNIR IEPPKKVPSA 430 440 450 460 470 480 DKRKSVFSLA ELGQMNGSVG GGGGAPGGGN GGGGGGGSGG GAGSGGAGGT SSGDDGEMPA 490 500 510 520 530 540 MHEIGEELIW TGRFFGGLCL DIKRKLPWFP SDFYDGFHIQ SISAILFIYL GCITNAITFG 550 560 570 580 590 600 GLLGDATDNY QGVMESFLGT AMAGSLFCLF SGQPLIILSS TGPILIFEKL LFDFSKGNGL 610 620 630 640 650 660 DYMEFRLWIG LHSAVQCLIL VATDASFIIK YITRFTEEGF STLISFIFIY DAIKKMIGAF 670 680 690 700 710 720 KYYPINMDFK PNFITTYKCE CVAPDTVNTT VFNASAPLAP DTNASLYNLL NLTALDWSLL 730 740 750 760 770 780 SKKECLSYGG RLLGNSCKFI PDLALMSFIL FFGTYSMTLT LKKFKFSRYF PTKVRALVAD 790 800 810 820 830 840 FSIVFSILMF CGIDACFGLE TPKLHVPSVI KPTRPDRGWF VAPFGKNPWW VYPASILPAL 850 860 870 880 890 900 LVTILIFMDQ QITAVIVNRK ENKLKKAAGY HLDLFWVGIL MALCSFMGLP WYVAATVISI 910 920 930 940 950 960 AHIDSLKMET ETSAPGEQPQ FLGVREQRVT GIIVFILTGI SVFLAPILKC IPLPVLYGVF 970 980 990 1000 1010 1020 LYMGVASLNG IQMGTGGSEF KIQKKLTPFW ERCKLFLMPA KHQPDHAFLR HVPLRRIHLF 1030 1040 1050 1060 1070 1080 TLVQILCLAV LWILKSTVAA IIFPVMSLST TDILGLIIVR RLLDFIFSQH DLAWIDNILP 1090 1100 1110 1120 1130 1140 EKEKKETDKK RKRKKGAHED CDEEPQFPPP SVIKIPMESV QSDPQNGIHC IARKRSSSWS YSL
	Isoform 3 (NBC4c) [UniParc]. Checksum: DC17DB7C69ABD27D	FASTA	1,121	124,551
	10 20 30 40 50 60 MKVKEEKAGV GKLDHTNHRR RFPDQKECPP IHIGLPVPTY PQRKTDQKGH LSGLQKVHWG 70 80 90 100 110 120 LRPDQPQQEL TGPGSGASSQ DSSMDLISRT RSPAAEQLQD ILGEEDEAPN PTLFTEMDTL 130 140 150 160 170 180 QHDGDQMEWK ESARWIKFEE KVEEGGERWS KPHVSTLSLH SLFELRTCLQ TGTVLLDLDS 190 200 210 220 230 240 GSLPQIIDDV IEKQIEDGLL RPELRERVSY VLLRRHRHQT KKPIHRSLAD IGKSVSTTNR 250 260 270 280 290 300 SPARSPGAGP SLHHSTEDLR MRQSANYGRL CHAQSRSMND ISLTPNTDQR KNKFMKKIPK 310 320 330 340 350 360 DSEASNVLVG EVDFLDQPFI AFVRLIQSAM LGGVTEVPVP TRFLFILLGP SGRAKSYNEI 370 380 390 400 410 420 GRAIATLMVD DLFSDVAYKA RNREDLIAGI DEFLDEVIVL PPGEWDPNIR IEPPKKVPSA 430 440 450 460 470 480 DKRKSVFSLA ELGQMNGSVG GGGGAPGGGN GGGGGGGSGG GAGSGGAGGT SSGDDGEMPA 490 500 510 520 530 540 MHEIGEELIW TGRFFGGLCL DIKRKLPWFP SDFYDGFHIQ SISAILFIYL GCITNAITFG 550 560 570 580 590 600 GLLGDATDNY QGVMESFLGT AMAGSLFCLF SGQPLIILSS TGPILIFEKL LFDFSKGNGL 610 620 630 640 650 660 DYMEFRLWIG LHSAVQCLIL VATDASFIIK YITRFTEEGF STLISFIFIY DAIKKMIGAF 670 680 690 700 710 720 KYYPINMDFK PNFITTYKCE CVAPDTVNTT VFNASAPLAP DTNASLYNLL NLTALDWSLL 730 740 750 760 770 780 SKKECLSYGG RLLGNSCKFI PDLALMSFIL FFGTYSMTLT LKKFKFSRYF PTKVRALVAD 790 800 810 820 830 840 FSIVFSILMF CGIDACFGLE TPKLHVPSVI KPTRPDRGWF VAPFGKNPWW VYPASILPAL 850 860 870 880 890 900 LVTILIFMDQ QITAVIVNRK ENKLKKAAGY HLDLFWVGIL MALCSFMGLP WYVAATVISI 910 920 930 940 950 960 AHIDSLKMET ETSAPGEQPQ FLGVREQRVT GIIVFILTGI SVFLAPILKC IPLPVLYGVF 970 980 990 1000 1010 1020 LYMGVASLNG IQFWERCKLF LMPAKHQPDH AFLRHVPLRR IHLFTLVQIL CLAVLWILKS 1030 1040 1050 1060 1070 1080 TVAAIIFPVM ILGLIIVRRL LDFIFSQHDL AWIDNILPEK EKKETDKKRK RKKGAHEDCD 1090 1100 1110 1120 EEPQFPPPSV IKIPMESVQS DPQNGIHCIA RKRSSSWSYS L
	Isoform 4 (NBC4d) [UniParc]. Checksum: FEE31CEF13F4F57B	FASTA	1,040	115,240
	10 20 30 40 50 60 MKVKEEKAGV GKLDHTNHRR RFPDQKECPP IHIGLPVPTY PQRKTDQKGH LSGLQKVHWG 70 80 90 100 110 120 LRPDQPQQEL TGPGSGASSQ DSSMDLISRT RSPAAEQLQD ILGEEDEAPN PTLFTEMDTL 130 140 150 160 170 180 QHDGDQMEWK ESARWIKFEE KVEEGGERWS KPHVSTLSLH SLFELRTCLQ TGTVLLDLDS 190 200 210 220 230 240 GSLPQIIDDV IEKQIEDGLL RPELRERVSY VLLRRHRHQT KKPIHRSLAD IGKSVSTTNR 250 260 270 280 290 300 SPARSPGAGP SLHHSTEDLR MRQSANYGRL CHAQSRSMND ISLTPNTDQR KNKFMKKIPK 310 320 330 340 350 360 DSEASNVLVG EVDFLDQPFI AFVRLIQSAM LGGVTEVPVP TRFLFILLGP SGRAKSYNEI 370 380 390 400 410 420 GRAIATLMVD DLFSDVAYKA RNREDLIAGI DEFLDEVIVL PPGEWDPNIR IEPPKKVPSA 430 440 450 460 470 480 DKRKSVFSLA ELGQMNGSVG GGGGAPGGGN GGGGGGGSGG GAGSGGAGGT SSGDDGEMPA 490 500 510 520 530 540 MHEIGEELIW TGRFFGGLCL DIKRKLPWFP SDFYDGFHIQ SISAILFIYL GCITNAITFG 550 560 570 580 590 600 GLLGDATDNY QGVMESFLGT AMAGSLFCLF SGQPLIILSS TGPILIFEKL LFDFSKGNGL 610 620 630 640 650 660 DYMEFRLWIG LHSAVQCLIL VATDASFIIK YITRFTEEGF STLISFIFIY DAIKKMIGAF 670 680 690 700 710 720 KYYPINMDFK PNFITTYKCE CVAPDTVNTT VFNASAPLAP DTNASLYNLL NLTALDWSLL 730 740 750 760 770 780 SKKECLSYGG RLLGNSCKFI PDLALMSFIL FFGTYSMTLT LKKFKFSRYF PTKVRALVAD 790 800 810 820 830 840 FSIVFSILMF CGIDACFGLE TPKLHVPSVI KPTRPDRGWF VAPFGKNPWW VYPASILPAL 850 860 870 880 890 900 LVTILIFMDQ QITAVIVNRK ENKLKKAAGY HLDLFWVGIL MALCSFMGLP WYVAATVISI 910 920 930 940 950 960 AHIDSLKMET ETSAPGEQPQ FLGVREQRVT GIIVFILTGI SVFLAPILKI LGLIIVRRLL 970 980 990 1000 1010 1020 DFIFSQHDLA WIDNILPEKE KKETDKKRKR KKGAHEDCDE EPQFPPPSVI KIPMESVQSD 1030 1040 PQNGIHCIAR KRSSSWSYSL
	Isoform 5 (isoform d variant) [UniParc]. Checksum: 9C45DA4AC127035F	FASTA	993	108,663
	10 20 30 40 50 60 MKVKEEKAGV GKLDHTNHRR RFPDQKECPP IHIGLPVPTY PQRKTDQKGH LSGLQKVHWG 70 80 90 100 110 120 LRPDQPQQEL TGPGSGASSQ DSSMDLIEHK GSPAAEQLQD ILGEEDEAPN PTLFTEMDTL 130 140 150 160 170 180 QHDGDQMEWK ESARWIKFEE KVEEGGERWS KPHVSTLSLH SLFELRTCLQ TGTVLLDLDS 190 200 210 220 230 240 GSLPQIIDDV IEKQIEDGLL RPELRERVSY VLLRRHRHQT KKPIHRSLAD IGKSVSTTNR 250 260 270 280 290 300 SPARSPGAGP SLHHSTEDLR MRQSANYGRL CHAQSRSMND ISLTPNTDQR KNKFMKKIPK 310 320 330 340 350 360 DSEASNVLVG EVDFLDQPFI AFVRLIQSAM LGGVTEVPVP TRFLFILLGP SGRAKSYNEI 370 380 390 400 410 420 GRAIATLMVD DLFSDVAYKA RNREDLIAGI DEFLDEVIVL PPGEWDPNIR IEPPKKVPSA 430 440 450 460 470 480 DKRKSVFSLA ELGQMNGSVG GGGGAPGGGN GGGGGGGSGG GAGSGGAGGT SSGDDGEMPA 490 500 510 520 530 540 MHEIGEELIW TGRFFGGLCL DIKRKLPWFP SDFYDGFHIQ SISAILFIYL GCITNAITFG 550 560 570 580 590 600 GLLGDATDNY QGVMESFLGT AMAGSLFCLF SGQPLIILSS TGPILIFEKL LFDFSKGNGL 610 620 630 640 650 660 DYMEFRLWIG LHSAVQCLIL VATDASFIIK YITRFTEEGF STLISFIFIY DAIKKMIGAF 670 680 690 700 710 720 KYYPINMDFK PNFITTYKCE CVAPDTVNTT VFNASAPLAP DTNASLYNLL NLTALDWSLL 730 740 750 760 770 780 SKKECLSYGG RLLGNSCKFI PDLALMSFIL FFGTYSMTLT LKKFKFSRYF PTKVRALVAD 790 800 810 820 830 840 FSIVFSILMF CGIDACFGLE TPKLHVPSVI KPTRPDRGWF VAPFGKNPWW VYPASILPAL 850 860 870 880 890 900 LVTILIFMDQ QITAVIVNRK ENKLKGTESN RHHRLHPDGN LCLPGSHPKV YPPAGAVRSL 910 920 930 940 950 960 PLHGRGLPEW HPVLGTLQAL PDASQAPAGP CLPAARAAAP DPPLHPGADP LPGGALDPQI 970 980 990 HGGCHHLPGH DPGPHHRSKA SGFHLFPARP GLD
	Isoform 6 (NBC4f) [UniParc]. Checksum: 61243CD7DF614154	FASTA	968	107,526
	10 20 30 40 50 60 MKVKEEKAGV GKLDHTNHRR RFPDQKECPP IHIGLPVPTY PQRKTDQKGH LSGLQKVHWG 70 80 90 100 110 120 LRPDQPQQEL TGPGSGASSQ DSSMDLISRT RSPAAEQLQD ILGEEDEAPN PTLFTEMDTL 130 140 150 160 170 180 QHDGDQMEWK ESARWIKFEE KVEEGGERWS KPHVSTLSLH SLFELRTCLQ TGTVLLDLDS 190 200 210 220 230 240 GSLPQIIDDV IEKQIEDGLL RPELRERVSY VLLRRHRHQT KKPIHRSLAD IGKSVSTTNR 250 260 270 280 290 300 SPARSPGAGP SLHHSTEDLR MRQSANYGRL CHAQSRSMND ISLTPNTDQR KNKFMKKIPK 310 320 330 340 350 360 DSEASNVLVG EVDFLDQPFI AFVRLIQSAM LGGVTEVPVP TRFLFILLGP SGRAKSYNEI 370 380 390 400 410 420 GRAIATLMVD DLFSDVAYKA RNREDLIAGI DEFLDEVIVL PPGEWDPNIR IEPPKKVPSA 430 440 450 460 470 480 DKRKSVFSLA ELGQMNGSVG GGGGAPGGGN GGGGGGGSGG GAGSGGAGGT SSGDDGEMPA 490 500 510 520 530 540 MHEIGEELIW TGRFFGGLCL DIKRKLPWFP SDFYDGFHIQ SISAILFIYL GCITNAITFG 550 560 570 580 590 600 GLLGDATDNY QGVMESFLGT AMAGSLFCLF SGQPLIILSS TGPILIFEKL LFDFSKGNGL 610 620 630 640 650 660 DYMEFRLWIG LHSAVQCLIL VATDASFIIK YITRFTEEGF STLISFIFIY DAIKKMIGAF 670 680 690 700 710 720 KYYPINMDFK PNFITTYKCE CVAPDTVNTT VFNASAPLAP DTNASLYNLL NLTALDWSLL 730 740 750 760 770 780 SKKECLSYGG RLLGNSCKFI PDLALMSFIL FFGTYSMTLT LKKFKFSRYF PTKPTRPDRG 790 800 810 820 830 840 WFVAPFGKNP WWVYPASILP ALLVTILIFM DQQITAVIVN RKENKLKREQ RVTGIIVFIL 850 860 870 880 890 900 TGISVFLAPI LKCIPLPVLY GVFLYMGVAS LNGIQFWERC KLFLMPAKHQ PDHAFLRHVP 910 920 930 940 950 960 LRRIHLFTLV QILCLAVLWI LKSTVAAIIF PVMSLSTTDI LGLIIVRRLL DFIFSQHDLA WIDKIEFP
	Isoform 7 [UniParc]. Checksum: 93AAC3C3E0649931	FASTA	1,019	113,390
	10 20 30 40 50 60 MKVKEEKAGV GKLDHTNHRR RFPDQKGSPA AEQLQDILGE EDEAPNPTLF TEMDTLQHDG 70 80 90 100 110 120 DQMEWKESAR WIKFEEKVEE GGERWSKPHV STLSLHSLFE LRTCLQTGTV LLDLDSGSLP 130 140 150 160 170 180 QIIDDVIEKQ IEDGLLRPEL RERVSYVLLR RHRHQTKKPI HRSLADIGKS VSTTNRSPAR 190 200 210 220 230 240 SPGAGPSLHH STEDLRMRQS ANYGRLCHAQ SRSMNDISLT PNTDQRKNKF MKKIPKDSEA 250 260 270 280 290 300 SNVLVGEVDF LDQPFIAFVR LIQSAMLGGV TEVPVPTRFL FILLGPSGRA KSYNEIGRAI 310 320 330 340 350 360 ATLMVDDLFS DVAYKARNRE DLIAGIDEFL DEVIVLPPGE WDPNIRIEPP KKVPSADKRK 370 380 390 400 410 420 SVFSLAELGQ MNGSVGGGGG APGGGNGGGG GGGSGGGAGS GGAGGTSSGD DGEMPAMHEI 430 440 450 460 470 480 GEELIWTGRF FGGLCLDIKR KLPWFPSDFY DGFHIQSISA ILFIYLGCIT NAITFGGLLG 490 500 510 520 530 540 DATDNYQGVM ESFLGTAMAG SLFCLFSGQP LIILSSTGPI LIFEKLLFDF SKGNGLDYME 550 560 570 580 590 600 FRLWIGLHSA VQCLILVATD ASFIIKYITR FTEEGFSTLI SFIFIYDAIK KMIGAFKYYP 610 620 630 640 650 660 INMDFKPNFI TTYKCECVAP DTVNTTVFNA SAPLAPDTNA SLYNLLNLTA LDWSLLSKKE 670 680 690 700 710 720 CLSYGGRLLG NSCKFIPDLA LMSFILFFGT YSMTLTLKKF KFSRYFPTKP TRPDRGWFVA 730 740 750 760 770 780 PFGKNPWWVY PASILPALLV TILIFMDQQI TAVIVNRKEN KLKKAAGYHL DLFWVGILMA 790 800 810 820 830 840 LCSFMGLPWY VAATVISIAH IDSLKMETET SAPGEQPQFL GVREQRVTGI IVFILTGISV 850 860 870 880 890 900 FLAPILKCIP LPVLYGVFLY MGVASLNGIQ FWERCKLFLM PAKHQPDHAF LRHVPLRRIH 910 920 930 940 950 960 LFTLVQILCL AVLWILKSTV AAIIFPVMIL GLIIVRRLLD FIFSQHDLAW IDNILPEKEK 970 980 990 1000 1010 KETDKKRKRK KGAHEDCDEE PQFPPPSVIK IPMESVQSDP QNGIHCIARK RSSSWSYSL
	Isoform 8 (NBC4e) [UniParc]. Checksum: 7D1BA0C19F65AC3F	FASTA	1,067	118,125
	10 20 30 40 50 60 MKVKEEKAGV GKLDHTNHRR RFPDQKECPP IHIGLPVPTY PQRKTDQKGH LSGLQKVHWG 70 80 90 100 110 120 LRPDQPQQEL TGPGSGASSQ DSSMDLISRT RSPAAEQLQD ILGEEDEAPN PTLFTEMDTL 130 140 150 160 170 180 QHDGDQMEWK ESARWIKFEE KVEEGGERWS KPHVSTLSLH SLFELRTCLQ TGTVLLDLDS 190 200 210 220 230 240 GSLPQIIDDV IEKQIEDGLL RPELRERVSY VLLRRHRHQT KKPIHRSLAD IGKSVSTTNR 250 260 270 280 290 300 SPARSPGAGP SLHHSTEDLR MRQSANYGRL CHAQSRSMND ISLTPNTDQR KNKFMKKIPK 310 320 330 340 350 360 DSEASNVLVG EVDFLDQPFI AFVRLIQSAM LGGVTEVPVP TRFLFILLGP SGRAKSYNEI 370 380 390 400 410 420 GRAIATLMVD DLFSDVAYKA RNREDLIAGI DEFLDEVIVL PPGEWDPNIR IEPPKKVPSA 430 440 450 460 470 480 DKRKSVFSLA ELGQMNGSVG GGGGAPGGGN GGGGGGGSGG GAGSGGAGGT SSGDDGEMPA 490 500 510 520 530 540 MHEIGEELIW TGRFFGGLCL DIKRKLPWFP SDFYDGFHIQ SISAILFIYL GCITNAITFG 550 560 570 580 590 600 GLLGDATDNY QGVMESFLGT AMAGSLFCLF SGQPLIILSS TGPILIFEKL LFDFSKGNGL 610 620 630 640 650 660 DYMEFRLWIG LHSAVQCLIL VATDASFIIK YITRFTEEGF STLISFIFIY DAIKKMIGAF 670 680 690 700 710 720 KYYPINMDFK PNFITTYKCE CVAPDTVNTT VFNASAPLAP DTNASLYNLL NLTALDWSLL 730 740 750 760 770 780 SKKECLSYGG RLLGNSCKFI PDLALMSFIL FFGTYSMTLT LKKFKFSRYF PTKVRALVAD 790 800 810 820 830 840 FSIVFSILMF CGIDACFGLE TPKLHVPSVI KPTRPDRGWF VAPFGKNPWW VYPASILPAL 850 860 870 880 890 900 LVTILIFMDQ QITAVIVNRK ENKLKKAAGY HLDLFWVGIL MALCSFMGLP WYVAATVISI 910 920 930 940 950 960 AHIDSLKMET ETSAPGEQPQ FLGVREQRVT GIIVFILTGI SVFLAPILKC IPLPVLYGVF 970 980 990 1000 1010 1020 LYMGVASLNG IQMGTGGSEF KIQKKLTPFW ERCKLFLMPA KHQPDHAFLR HVPLRRIHLF 1030 1040 1050 1060 TLVQILCLAV LWILKSTVAA IIFPVMILGL IIVRRLLDFI FSQHDLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization and chromosomal assignment of NBC4, a new member of the sodium bicarbonate cotransporter family." Pushkin A., Abuladze N., Newman D., Lee I., Xu G., Kurtz I. Biochim. Biophys. Acta 1493:215-218(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Heart.
[2]	"Two C-terminal variants of NBC4, a new member of the sodium bicarbonate cotransporter family: cloning, characterization, and localization." Pushkin A., Abuladze N., Newman D., Lee I., Xu G., Kurtz I. IUBMB Life 50:13-19(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY. Tissue: Heart and Testis.
[3]	"Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued)." Pushkin A., Abuladze N., Newman D., Tatishchev S., Kurtz I. Cytogenet. Cell Genet. 95:163-168(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4). Tissue: Testis.
[4]	"Functional characterization of NBC4: a new electrogenic sodium-bicarbonate cotransporter." Sassani P., Pushkin A., Gross E., Gomer A., Abuladze N., Dukkipati R., Carpenito G., Kurtz I. Am. J. Physiol. 282:C408-C416(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY, INHIBITION. Tissue: Testis.
[5]	"Functional characterization of human NBC4 as an electrogenic Na+-HCO cotransporter (NBCe2)." Virkki L.V., Wilson D.A., Vaughan-Jones R.D., Boron W.F. Am. J. Physiol. 282:C1278-C1289(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, INHIBITION.
[6]	"Expression of the Na+-HCO-3 cotransporter NBC4 in rat kidney and characterization of a novel NBC4 variant." Xu J., Wang Z., Barone S., Petrovic M., Amlal H., Conforti L., Petrovic S., Soleimani M. Am. J. Physiol. 284:F41-F50(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6), NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 8), FUNCTION, INHIBITION. Tissue: Liver.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 7).
[9]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 88-993 (ISOFORM 5), VARIANT TYR-253. Tissue: Brain.
[10]	"Molecular expression of SLC4-derived Na+-dependent anion transporters in selected human tissues." Damkier H.H., Nielsen S., Praetorius J. Am. J. Physiol. 293:R2136-R2146(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF207661 mRNA. Translation: AAG18492.1. Frameshift. AF243499 mRNA. Translation: AAK26741.1. AF293338 mRNA. Translation: AAK97073.1. AF293337 mRNA. Translation: AAK97072.1. AF452248 mRNA. Translation: AAL48291.1. AF453528 mRNA. Translation: AAL50802.1. Frameshift. CH471053 Genomic DNA. Translation: EAW99690.1. CH471053 Genomic DNA. Translation: EAW99691.1. Frameshift. CH471053 Genomic DNA. Translation: EAW99693.1. BC109221 mRNA. Translation: AAI09222.1. AB209752 mRNA. Translation: BAD92989.1.
IPI	IPI00061148. IPI00107529. IPI00218089. IPI00655610. IPI00889641. IPI00889661. IPI00889759. IPI00889761.
RefSeq	NP_067019.3. NM_021196.3. NP_597812.1. NM_133478.2.
UniGene	Hs.744095.
3D structure databases
ProteinModelPortal	Q9BY07.
SMR	Q9BY07. Positions 111-428, 505-546.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6489517.
STRING	9606.ENSP00000350475.
Protein family/group databases
TCDB	2.A.31.2.8. anion exchanger (AE) family.
PTM databases
PhosphoSite	Q9BY07.
Polymorphism databases
DMDM	182691595.
Proteomic databases
PaxDb	Q9BY07.
PRIDE	Q9BY07.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000346834; ENSP00000251768; ENSG00000188687. ENST00000357822; ENSP00000350475; ENSG00000188687. ENST00000358683; ENSP00000351513; ENSG00000188687. ENST00000359484; ENSP00000352461; ENSG00000188687. ENST00000377632; ENSP00000366859; ENSG00000188687. ENST00000377634; ENSP00000366861; ENSG00000188687. ENST00000394019; ENSP00000377587; ENSG00000188687.
GeneID	57835.
KEGG	hsa:57835.
UCSC	uc002skn.3. human. uc002sko.1. human. uc002skp.1. human. uc010ffc.1. human.
Organism-specific databases
CTD	57835.
GeneCards	GC02M074443.
HGNC	HGNC:18168. SLC4A5.
HPA	HPA036621.
MIM	606757. gene.
neXtProt	NX_Q9BY07.
PharmGKB	PA164742397.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG318437.
HOVERGEN	HBG004326.
InParanoid	Q9BY07.
KO	K13857.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q9BY07.
Bgee	Q9BY07.
CleanEx	HS_SLC4A5.
Genevestigator	Q9BY07.
Family and domain databases
Gene3D	3.40.1100.10. 1 hit.
InterPro	IPR013769. Band3_cytoplasmic_dom. IPR011531. HCO3_transpt_C. IPR003020. HCO3_transpt_euk. IPR003024. Na/HCO3_transpt. IPR016152. PTrfase/Anion_transptr. [Graphical view]
PANTHER	PTHR11453. PTHR11453. 1 hit.
Pfam	PF07565. Band_3_cyto. 1 hit. PF00955. HCO3_cotransp. 1 hit. [Graphical view]
PRINTS	PR01231. HCO3TRNSPORT. PR01232. NAHCO3TRSPRT.
SUPFAM	SSF55804. PTrfase/Anion_transptr. 1 hit.
TIGRFAMs	TIGR00834. ae. 1 hit.
PROSITE	PS00219. ANION_EXCHANGER_1. False negative. PS00220. ANION_EXCHANGER_2. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC4A5. human.
GenomeRNAi	57835.
NextBio	64831.
SOURCE	Search...

Entry information

Entry name

S4A5_HUMAN

Accession

Primary (citable) accession number: Q9BY07
Secondary accession number(s): Q32MA7 , Q59EQ9, Q8WXD3, Q8WXD7, Q96DS7, Q96DS8, Q9HBU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	May 29, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.