Q9BXW4 (MLP3C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microtubule-associated proteins 1A/1B light chain 3C

Alternative name(s):
Autophagy-related protein LC3 C
Autophagy-related ubiquitin-like modifier LC3 C
MAP1 light chain 3-like protein 3
MAP1A/MAP1B light chain 3 C
Short name=MAP1A/MAP1B LC3 C
Microtubule-associated protein 1 light chain 3 gamma

Gene names

Name:

MAP1LC3C

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in formation of autophagosomal vacuoles (autophagosomes) By similarity.
Subunit structure	3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins By similarity. Interacts with TP53INP1 and TP53INP2. Ref.5
Subcellular location	Cytoplasm › cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes. Ref.1
Tissue specificity	Most abundant in placenta, lung and ovary. Ref.1
Post-translational modification	The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Ref.4
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Autophagy Ubl conjugation pathway
Cellular component	Cytoplasm Cytoplasmic vesicle Cytoskeleton Membrane Microtubule
PTM	Lipoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	autophagic vacuole Inferred from sequence or structural similarity Ref.1. Source: UniProtKB autophagic vacuole membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW organelle membrane Inferred from sequence or structural similarity Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG101	Q9BSB4	2	EBI-2603996,EBI-2946739
ATG16L1	Q676U5	4	EBI-2603996,EBI-535909
DYX1C1	Q8WXU2	2	EBI-2603996,EBI-2946907
FYCO1	Q9BQS8	2	EBI-2603996,EBI-2869338
GBAS	O75323	2	EBI-2603996,EBI-307133
IPO5	O00410	2	EBI-2603996,EBI-356424
KBTBD6	Q86V97	2	EBI-2603996,EBI-2514778
KBTBD7	Q8WVZ9	2	EBI-2603996,EBI-473695
NBR1	Q14596	3	EBI-2603996,EBI-742698
NEK9	Q8TD19	2	EBI-2603996,EBI-1044009
RASSF1	Q9NS23	2	EBI-2603996,EBI-367363
RASSF5	Q8WWW0	2	EBI-2603996,EBI-367390
SF3A1	Q15459	3	EBI-2603996,EBI-1054743
SQSTM1	Q13501	2	EBI-2603996,EBI-307104
STK3	Q13188	2	EBI-2603996,EBI-992580
TBC1D2B	Q9UPU7	2	EBI-2603996,EBI-2947180
TECPR2	O15040	2	EBI-2603996,EBI-2946991
UBA5	Q9GZZ9	2	EBI-2603996,EBI-747805
ULK1	O75385	2	EBI-2603996,EBI-908831

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 126	126	Microtubule-associated proteins 1A/1B light chain 3C		PRO_0000017204
	Propeptide	127 – 147	21	Removed in mature form		PRO_0000017205
Amino acid modifications
	Lipidation	126	1	Phosphatidylethanolamine amidated glycine Probable
Experimental info
	Mutagenesis	126	1	G → A: No processing of precursor. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9BXW4 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D3DFA8EE5985C3AA	FASTA	147	16,852
	10 20 30 40 50 60 MPPPQKIPSV RPFKQRKSLA IRQEEVAGIR AKFPNKIPVV VERYPRETFL PPLDKTKFLV 70 80 90 100 110 120 PQELTMTQFL SIIRSRMVLR ATEAFYLLVN NKSLVSMSAT MAEIYRDYKD EDGFVYMTYA 130 140 SQETFGCLES AAPRDGSSLE DRPCNPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y., Ling W., Wu C., Zhao S., Liu J.O., Yu L. J. Biol. Chem. 278:29278-29287(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-126.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." Tanida I., Sou Y.-S., Ezaki J., Minematsu-Ikeguchi N., Ueno T., Kominami E. J. Biol. Chem. 279:36268-36276(2004) [PubMed] [Europe PMC] [Abstract] Cited for: LIPIDATION, CLEAVAGE BY APG4B.
[5]	"DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription." Sancho A., Duran J., Garcia-Espana A., Mauvezin C., Alemu E.A., Lamark T., Macias M.J., Desalle R., Royo M., Sala D., Chicote J.U., Palacin M., Johansen T., Zorzano A. PLoS ONE 7:E34034-E34034(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TP53INP1 AND TP53INP2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF276659 mRNA. Translation: AAK35152.1.
BX571673 Genomic DNA. Translation: CAH72477.1.
BC127722 mRNA. Translation: AAI27723.1.
BC132986 mRNA. Translation: AAI32987.1.
BC132988 mRNA. Translation: AAI32989.1.

IPI

IPI00387017.

RefSeq

NP_001004343.1. NM_001004343.2.

UniGene

Hs.534971.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3VVW	X-ray	2.50	B	1-126	[»]

ProteinModelPortal

Q9BXW4.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9BXW4. 51 interactions.

STRING

9606.ENSP00000349785.

Polymorphism databases

DMDM

84028113.

Proteomic databases

PaxDb

Q9BXW4.

PRIDE

Q9BXW4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000357246; ENSP00000349785; ENSG00000197769.

GeneID

440738.

KEGG

hsa:440738.

UCSC

uc001hzk.2. human.

Organism-specific databases

CTD

440738.

GeneCards

GC01M242158.

HGNC

HGNC:13353. MAP1LC3C.

MIM

609605. gene.

neXtProt

NX_Q9BXW4.

PharmGKB

PA142671482.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG245878.

HOGENOM

HOG000232034.

HOVERGEN

HBG051706.

InParanoid

Q9BXW4.

KO

K10435.

OMA

IIRNRMS.

OrthoDB

EOG4GQQ6D.

PhylomeDB

Q9BXW4.

Gene expression databases

Bgee

Q9BXW4.

CleanEx

HS_MAP1LC3C.

Genevestigator

Q9BXW4.

GermOnline

ENSG00000197769. Homo sapiens.

Family and domain databases

InterPro

IPR004241. Atg8_ubiquitin-like.
[Graphical view]

PANTHER

PTHR10969. PTHR10969. 1 hit.

Pfam

PF02991. Atg8. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

440738.

NextBio

109492.

SOURCE

Search...

Entry information

Entry name

MLP3C_HUMAN

Accession

Primary (citable) accession number: Q9BXW4
Secondary accession number(s): A0PJY8, A2RUP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.