Q9BXS5 (AP1M1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-1 complex subunit mu-1

Alternative name(s):
AP-mu chain family member mu1A
Adaptor protein complex AP-1 mu-1 subunit
Adaptor-related protein complex 1 mu-1 subunit
Clathrin assembly protein complex 1 medium chain 1
Clathrin coat assembly protein AP47
Clathrin coat-associated protein AP47
Golgi adaptor HA1/AP1 adaptin mu-1 subunit
Mu-adaptin 1
Mu1A-adaptin

Gene names

Name:	AP1M1
Synonyms:	CLTNM

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit structure	Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCH11 By similarity. Interacts with HIV-1 Nef. Associates with the AP1(MU)-Nef-MHC-I complex; this complex is required for MHC-I internalization.
Subcellular location	Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Post-translational modification	Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals By similarity.
Sequence similarities	Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular membrane organization Traceable author statement. Source: Reactome endosome to melanosome transport Inferred from mutant phenotype. Source: UniProtKB interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Inferred from electronic annotation. Source: InterPro melanosome organization Inferred from mutant phenotype. Source: UniProtKB post-Golgi vesicle-mediated transport Traceable author statement. Source: Reactome regulation of defense response to virus by virus Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular component	Golgi membrane Traceable author statement. Source: Reactome clathrin adaptor complex Inferred from electronic annotation. Source: InterPro clathrin coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome lysosomal membrane Traceable author statement. Source: Reactome
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LDOC1	O95751	3	EBI-541426,EBI-740738
MTF1	Q14872	3	EBI-541426,EBI-747024

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 423	422	AP-1 complex subunit mu-1		PRO_0000193770
Regions
	Domain	165 – 422	258	MHD
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.3
	Modified residue	152	1	Phosphothreonine By similarity
	Modified residue	154	1	Phosphothreonine By similarity
Natural variations
	Natural variant	303	1	R → Q in a breast cancer sample; somatic mutation. Ref.5		VAR_036536

Sequences

	Sequence		Length	Mass (Da)
	Q9BXS5 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 64EC5E47EA6F8E98	FASTA	423	48,587
	10 20 30 40 50 60 MSASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL AHGGVRFMWI 70 80 90 100 110 120 KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE ESIRDNFVII YELLDELMDF 130 140 150 160 170 180 GYPQTTDSKI LQEYITQEGH KLETGAPRPP ATVTNAVSWR SEGIKYRKNE VFLDVIESVN 190 200 210 220 230 240 LLVSANGNVL RSEIVGSIKM RVFLSGMPEL RLGLNDKVLF DNTGRGKSKS VELEDVKFHQ 250 260 270 280 290 300 CVRLSRFEND RTISFIPPDG EFELMSYRLN THVKPLIWIE SVIEKHSHSR IEYMIKAKSQ 310 320 330 340 350 360 FKRRSTANNV EIHIPVPNDA DSPKFKTTVG SVKWVPENSE IVWSIKSFPG GKEYLMRAHF 370 380 390 400 410 420 GLPSVEAEDK EGKPPISVKF EIPYFTTSGI QVRYLKIIEK SGYQALPWVR YITQNGDYQL RTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human clathrin-associated protein AP47 mRNA." Qu X., Zhai Y., Zhang C., Yu Y., Xing G., Wei H., Wu S., Zhou G., He F. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[3]	Bienvenut W.V., Claeys D. Submitted (JAN-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-12; 41-56; 87-96; 130-160; 202-211; 218-225; 380-393 AND 401-421, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: Platelet.
[4]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-303.
[6]	"The tyrosine binding pocket in the adaptor protein 1 (AP-1) mu1 subunit is necessary for Nef to recruit AP-1 to the major histocompatibility complex class I cytoplasmic tail." Wonderlich E.R., Williams M., Collins K.L. J. Biol. Chem. 283:3011-3022(2008) [PubMed: 18073204] [Abstract] Cited for: IDENTIFICATION IN A AP1(MU)-NEF-MHC-I COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF290613 mRNA. Translation: AAK28024.1. BC017469 mRNA. Translation: AAH17469.1.
IPI	IPI00032516.
RefSeq	NP_115882.1. NM_032493.3.
UniGene	Hs.71040.
3D structure databases
ProteinModelPortal	Q9BXS5.
SMR	Q9BXS5. Positions 2-423.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BXS5. 11 interactions.
MINT	MINT-1456291.
STRING	Q9BXS5.
PTM databases
PhosphoSite	Q9BXS5.
Polymorphism databases
DMDM	18202738.
Proteomic databases
PeptideAtlas	Q9BXS5.
PRIDE	Q9BXS5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000291439; ENSP00000291439; ENSG00000072958.
GeneID	8907.
KEGG	hsa:8907.
UCSC	uc002ndu.1. human.
Organism-specific databases
CTD	8907.
GeneCards	GC19P016308.
H-InvDB	HIX0202741.
HGNC	HGNC:13667. AP1M1.
HPA	HPA045256.
MIM	603535. gene.
neXtProt	NX_Q9BXS5.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00530000062779.
HOVERGEN	HBG050516.
InParanoid	Q9BXS5.
OrthoDB	EOG4CJVH3.
PhylomeDB	Q9BXS5.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking. REACT_6185. HIV Infection.
Gene expression databases
ArrayExpress	Q9BXS5.
Bgee	Q9BXS5.
CleanEx	HS_AP1M1.
Genevestigator	Q9BXS5.
GermOnline	ENSG00000072958. Homo sapiens.
Family and domain databases
InterPro	IPR022775. AP_mu_sigma_su. IPR001392. Clathrin_mu. IPR008968. Clathrin_mu_C. IPR018240. Clathrin_mu_CS. IPR011012. Longin-like. [Graphical view]
KO	K12393.
Pfam	PF00928. Adap_comp_sub. 1 hit. PF01217. Clat_adaptor_s. 1 hit. [Graphical view]
PIRSF	PIRSF005992. Clathrin_mu. 1 hit.
PRINTS	PR00314. CLATHRINADPT.
SUPFAM	SSF49447. AP50. 1 hit. SSF64356. Longin_like. 1 hit.
PROSITE	PS00990. CLAT_ADAPTOR_M_1. 1 hit. PS00991. CLAT_ADAPTOR_M_2. 1 hit. PS51072. MHD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	33467.
SOURCE	Search...

Entry information

Entry name

AP1M1_HUMAN

Accession

Primary (citable) accession number: Q9BXS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.