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Q9BXS5

- AP1M1_HUMAN

UniProt

Q9BXS5 - AP1M1_HUMAN

Protein

AP-1 complex subunit mu-1

Gene

AP1M1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
    2. endosome to melanosome transport Source: UniProtKB
    3. intracellular protein transport Source: InterPro
    4. melanosome organization Source: UniProtKB
    5. membrane organization Source: Reactome
    6. post-Golgi vesicle-mediated transport Source: Reactome
    7. regulation of defense response to virus by virus Source: Reactome
    8. viral process Source: Reactome

    Keywords - Biological processi

    Host-virus interaction, Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
    REACT_121399. MHC class II antigen presentation.
    REACT_19287. Lysosome Vesicle Biogenesis.
    REACT_19400. Golgi Associated Vesicle Biogenesis.
    SignaLinkiQ9BXS5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-1 complex subunit mu-1
    Alternative name(s):
    AP-mu chain family member mu1A
    Adaptor protein complex AP-1 subunit mu-1
    Adaptor-related protein complex 1 subunit mu-1
    Clathrin assembly protein complex 1 mu-1 medium chain 1
    Clathrin coat assembly protein AP47
    Clathrin coat-associated protein AP47
    Golgi adaptor HA1/AP1 adaptin mu-1 subunit
    Mu-adaptin 1
    Mu1A-adaptin
    Gene namesi
    Name:AP1M1
    Synonyms:CLTNM
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:13667. AP1M1.

    Subcellular locationi

    Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

    GO - Cellular componenti

    1. clathrin adaptor complex Source: InterPro
    2. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
    3. cytoplasmic vesicle membrane Source: Reactome
    4. cytosol Source: Reactome
    5. extracellular vesicular exosome Source: UniProt
    6. Golgi membrane Source: Reactome
    7. lysosomal membrane Source: Reactome
    8. membrane Source: UniProtKB
    9. trans-Golgi network membrane Source: Reactome

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24848.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 423422AP-1 complex subunit mu-1PRO_0000193770Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications
    Modified residuei152 – 1521PhosphothreonineBy similarity
    Modified residuei154 – 1541PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9BXS5.
    PaxDbiQ9BXS5.
    PeptideAtlasiQ9BXS5.
    PRIDEiQ9BXS5.

    PTM databases

    PhosphoSiteiQ9BXS5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9BXS5.
    BgeeiQ9BXS5.
    CleanExiHS_AP1M1.
    GenevestigatoriQ9BXS5.

    Organism-specific databases

    HPAiHPA045256.

    Interactioni

    Subunit structurei

    Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCH11 By similarity. Interacts with HIV-1 Nef. Associates with the AP1(MU)-Nef-MHC-I complex; this complex is required for MHC-I internalization.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LDOC1O957513EBI-541426,EBI-740738
    MTF1Q148723EBI-541426,EBI-747024

    Protein-protein interaction databases

    BioGridi114421. 28 interactions.
    IntActiQ9BXS5. 15 interactions.
    MINTiMINT-1456291.
    STRINGi9606.ENSP00000388996.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BXS5.
    SMRiQ9BXS5. Positions 2-423.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini168 – 421254MHDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG315786.
    HOGENOMiHOG000173247.
    HOVERGENiHBG050516.
    InParanoidiQ9BXS5.
    KOiK12393.
    OMAiYPWVRYI.
    PhylomeDBiQ9BXS5.
    TreeFamiTF300393.

    Family and domain databases

    InterProiIPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view]
    PfamiPF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
    PRINTSiPR00314. CLATHRINADPT.
    SUPFAMiSSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9BXS5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSASAVYVLD LKGKVLICRN YRGDVDMSEV EHFMPILMEK EEEGMLSPIL    50
    AHGGVRFMWI KHNNLYLVAT SKKNACVSLV FSFLYKVVQV FSEYFKELEE 100
    ESIRDNFVII YELLDELMDF GYPQTTDSKI LQEYITQEGH KLETGAPRPP 150
    ATVTNAVSWR SEGIKYRKNE VFLDVIESVN LLVSANGNVL RSEIVGSIKM 200
    RVFLSGMPEL RLGLNDKVLF DNTGRGKSKS VELEDVKFHQ CVRLSRFEND 250
    RTISFIPPDG EFELMSYRLN THVKPLIWIE SVIEKHSHSR IEYMIKAKSQ 300
    FKRRSTANNV EIHIPVPNDA DSPKFKTTVG SVKWVPENSE IVWSIKSFPG 350
    GKEYLMRAHF GLPSVEAEDK EGKPPISVKF EIPYFTTSGI QVRYLKIIEK 400
    SGYQALPWVR YITQNGDYQL RTQ 423
    Length:423
    Mass (Da):48,587
    Last modified:January 23, 2007 - v3
    Checksum:i64EC5E47EA6F8E98
    GO
    Isoform 2 (identifier: Q9BXS5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         182-182: L → LGKYPGVGWLGHT

    Note: No experimental confirmation available.

    Show »
    Length:435
    Mass (Da):49,840
    Checksum:iEEA5A4B9F17D86D9
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti303 – 3031R → Q in a breast cancer sample; somatic mutation. 1 Publication
    VAR_036536

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei182 – 1821L → LGKYPGVGWLGHT in isoform 2. 1 PublicationVSP_042542

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF290613 mRNA. Translation: AAK28024.1.
    DQ059565 mRNA. Translation: AAY54246.1.
    AC020911 Genomic DNA. No translation available.
    BC017469 mRNA. Translation: AAH17469.1.
    CCDSiCCDS12342.1. [Q9BXS5-1]
    CCDS46008.1. [Q9BXS5-2]
    RefSeqiNP_001123996.1. NM_001130524.1. [Q9BXS5-2]
    NP_115882.1. NM_032493.3. [Q9BXS5-1]
    UniGeneiHs.71040.

    Genome annotation databases

    EnsembliENST00000291439; ENSP00000291439; ENSG00000072958. [Q9BXS5-1]
    ENST00000444449; ENSP00000388996; ENSG00000072958. [Q9BXS5-2]
    GeneIDi8907.
    KEGGihsa:8907.
    UCSCiuc002ndu.2. human. [Q9BXS5-1]
    uc002ndv.2. human. [Q9BXS5-2]

    Polymorphism databases

    DMDMi18202738.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF290613 mRNA. Translation: AAK28024.1 .
    DQ059565 mRNA. Translation: AAY54246.1 .
    AC020911 Genomic DNA. No translation available.
    BC017469 mRNA. Translation: AAH17469.1 .
    CCDSi CCDS12342.1. [Q9BXS5-1 ]
    CCDS46008.1. [Q9BXS5-2 ]
    RefSeqi NP_001123996.1. NM_001130524.1. [Q9BXS5-2 ]
    NP_115882.1. NM_032493.3. [Q9BXS5-1 ]
    UniGenei Hs.71040.

    3D structure databases

    ProteinModelPortali Q9BXS5.
    SMRi Q9BXS5. Positions 2-423.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114421. 28 interactions.
    IntActi Q9BXS5. 15 interactions.
    MINTi MINT-1456291.
    STRINGi 9606.ENSP00000388996.

    PTM databases

    PhosphoSitei Q9BXS5.

    Polymorphism databases

    DMDMi 18202738.

    Proteomic databases

    MaxQBi Q9BXS5.
    PaxDbi Q9BXS5.
    PeptideAtlasi Q9BXS5.
    PRIDEi Q9BXS5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000291439 ; ENSP00000291439 ; ENSG00000072958 . [Q9BXS5-1 ]
    ENST00000444449 ; ENSP00000388996 ; ENSG00000072958 . [Q9BXS5-2 ]
    GeneIDi 8907.
    KEGGi hsa:8907.
    UCSCi uc002ndu.2. human. [Q9BXS5-1 ]
    uc002ndv.2. human. [Q9BXS5-2 ]

    Organism-specific databases

    CTDi 8907.
    GeneCardsi GC19P016308.
    HGNCi HGNC:13667. AP1M1.
    HPAi HPA045256.
    MIMi 603535. gene.
    neXtProti NX_Q9BXS5.
    PharmGKBi PA24848.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG315786.
    HOGENOMi HOG000173247.
    HOVERGENi HBG050516.
    InParanoidi Q9BXS5.
    KOi K12393.
    OMAi YPWVRYI.
    PhylomeDBi Q9BXS5.
    TreeFami TF300393.

    Enzyme and pathway databases

    Reactomei REACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
    REACT_121399. MHC class II antigen presentation.
    REACT_19287. Lysosome Vesicle Biogenesis.
    REACT_19400. Golgi Associated Vesicle Biogenesis.
    SignaLinki Q9BXS5.

    Miscellaneous databases

    GeneWikii AP1M1.
    GenomeRNAii 8907.
    NextBioi 33467.
    PROi Q9BXS5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BXS5.
    Bgeei Q9BXS5.
    CleanExi HS_AP1M1.
    Genevestigatori Q9BXS5.

    Family and domain databases

    InterProi IPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view ]
    Pfami PF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005992. Clathrin_mu. 1 hit.
    PRINTSi PR00314. CLATHRINADPT.
    SUPFAMi SSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEi PS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human clathrin-associated protein AP47 mRNA."
      Qu X., Zhai Y., Zhang C., Yu Y., Xing G., Wei H., Wu S., Zhou G., He F.
      Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Liver.
    2. Zhang H.-T., Burakoff S.J., Jin Y.-J.
      Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: T-cell.
    3. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Uterus.
    5. Bienvenut W.V., Claeys D.
      Submitted (JAN-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-12; 41-56; 87-96; 130-160; 202-211; 218-225; 380-393 AND 401-421, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Platelet.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-303.
    9. "The tyrosine binding pocket in the adaptor protein 1 (AP-1) mu1 subunit is necessary for Nef to recruit AP-1 to the major histocompatibility complex class I cytoplasmic tail."
      Wonderlich E.R., Williams M., Collins K.L.
      J. Biol. Chem. 283:3011-3022(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A AP1(MU)-NEF-MHC-I COMPLEX.

    Entry informationi

    Entry nameiAP1M1_HUMAN
    AccessioniPrimary (citable) accession number: Q9BXS5
    Secondary accession number(s): Q4TTY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3