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Q9BXR0

- TGT_HUMAN

UniProt

Q9BXR0 - TGT_HUMAN

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Protein

Queuine tRNA-ribosyltransferase

Gene

QTRT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By similarity).By similarity

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei106 – 1061SubstrateBy similarity
Active sitei279 – 2791NucleophileBy similarity
Metal bindingi317 – 3171ZincBy similarity
Metal bindingi319 – 3191ZincBy similarity
Metal bindingi322 – 3221ZincBy similarity
Metal bindingi348 – 3481ZincBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. queuine tRNA-ribosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. queuosine biosynthetic process Source: UniProtKB
  2. tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferase (EC:2.4.2.29)
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene namesi
Name:QTRT1
Synonyms:TGT, TGUT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:23797. QTRT1.

Subcellular locationi

Cytoplasm By similarity. Mitochondrion By similarity. Nucleus By similarity
Note: Weakly associates with mitochondria, possibly via QTRTD1.By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134963074.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 403402Queuine tRNA-ribosyltransferasePRO_0000135565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9BXR0.
PaxDbiQ9BXR0.
PRIDEiQ9BXR0.

PTM databases

PhosphoSiteiQ9BXR0.

Expressioni

Gene expression databases

BgeeiQ9BXR0.
CleanExiHS_QTRT1.
ExpressionAtlasiQ9BXR0. baseline and differential.
GenevestigatoriQ9BXR0.

Organism-specific databases

HPAiHPA048651.
HPA049440.

Interactioni

Subunit structurei

Interacts with QTRTD1.By similarity

Protein-protein interaction databases

BioGridi123623. 17 interactions.
IntActiQ9BXR0. 7 interactions.
MINTiMINT-4656737.
STRINGi9606.ENSP00000250237.

Structurei

3D structure databases

ProteinModelPortaliQ9BXR0.
SMRiQ9BXR0. Positions 27-376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0343.
GeneTreeiENSGT00530000063679.
HOGENOMiHOG000223473.
HOVERGENiHBG101131.
InParanoidiQ9BXR0.
KOiK00773.
OMAiSSCCAPF.
OrthoDBiEOG7W41BR.
PhylomeDBiQ9BXR0.
TreeFamiTF300732.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PANTHERiPTHR11962. PTHR11962. 1 hit.
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9BXR0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGAATQASL ESAPRIMRLV AECSRSRARA GELWLPHGTV ATPVFMPVGT
60 70 80 90 100
QATMKGITTE QLDALGCRIC LGNTYHLGLR PGPELIQKAN GLHGFMNWPH
110 120 130 140 150
NLLTDSGGFQ MVSLVSLSEV TEEGVRFRSP YDGNETLLSP EKSVQIQNAL
160 170 180 190 200
GSDIIMQLDD VVSSTVTGPR VEEAMYRSIR WLDRCIAAHQ RPDKQNLFAI
210 220 230 240 250
IQGGLDADLR ATCLEEMTKR DVPGFAIGGL SGGESKSQFW RMVALSTSRL
260 270 280 290 300
PKDKPRYLMG VGYATDLVVC VALGCDMFDC VFPTRTARFG SALVPTGNLQ
310 320 330 340 350
LRKKVFEKDF GPIDPECTCP TCQKHSRAFL HALLHSDNTA ALHHLTVHNI
360 370 380 390 400
AYQLQLMSAV RTSIVEKRFP DFVRDFMGAM YGDPTLCPTW ATDALASVGI

TLG
Length:403
Mass (Da):44,048
Last modified:June 16, 2009 - v3
Checksum:i1E30F3C7BF47A106
GO
Isoform 2 (identifier: Q9BXR0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-225: SIRWLDRCIA...EMTKRDVPGF → PLGLVTGALL...AGRRSPGHLP
     226-403: Missing.

Note: No experimental confirmation available.

Show »
Length:225
Mass (Da):24,015
Checksum:iC62B238F305E24A9
GO

Sequence cautioni

The sequence AAH15350.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei178 – 22548SIRWL…DVPGF → PLGLVTGALLQVNPLAGPVH CSPSAAGQAEPLRHYPGWAG RRSPGHLP in isoform 2. 1 PublicationVSP_056470Add
BLAST
Alternative sequencei226 – 403178Missing in isoform 2. 1 PublicationVSP_056471Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302783 mRNA. Translation: AAG60033.1.
AF302784 mRNA. Translation: AAG60034.1.
AK294166 mRNA. Translation: BAG57488.1.
AC011475 Genomic DNA. No translation available.
BC015350 mRNA. Translation: AAH15350.1. Different initiation.
CCDSiCCDS12248.1. [Q9BXR0-1]
RefSeqiNP_112486.1. NM_031209.2.
UniGeneiHs.631638.

Genome annotation databases

EnsembliENST00000250237; ENSP00000250237; ENSG00000213339. [Q9BXR0-1]
ENST00000421333; ENSP00000389126; ENSG00000213339. [Q9BXR0-2]
GeneIDi81890.
KEGGihsa:81890.
UCSCiuc002mpr.3. human. [Q9BXR0-1]

Polymorphism databases

DMDMi239938842.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302783 mRNA. Translation: AAG60033.1 .
AF302784 mRNA. Translation: AAG60034.1 .
AK294166 mRNA. Translation: BAG57488.1 .
AC011475 Genomic DNA. No translation available.
BC015350 mRNA. Translation: AAH15350.1 . Different initiation.
CCDSi CCDS12248.1. [Q9BXR0-1 ]
RefSeqi NP_112486.1. NM_031209.2.
UniGenei Hs.631638.

3D structure databases

ProteinModelPortali Q9BXR0.
SMRi Q9BXR0. Positions 27-376.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123623. 17 interactions.
IntActi Q9BXR0. 7 interactions.
MINTi MINT-4656737.
STRINGi 9606.ENSP00000250237.

Chemistry

BindingDBi Q9BXR0.
ChEMBLi CHEMBL6004.

PTM databases

PhosphoSitei Q9BXR0.

Polymorphism databases

DMDMi 239938842.

Proteomic databases

MaxQBi Q9BXR0.
PaxDbi Q9BXR0.
PRIDEi Q9BXR0.

Protocols and materials databases

DNASUi 81890.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000250237 ; ENSP00000250237 ; ENSG00000213339 . [Q9BXR0-1 ]
ENST00000421333 ; ENSP00000389126 ; ENSG00000213339 . [Q9BXR0-2 ]
GeneIDi 81890.
KEGGi hsa:81890.
UCSCi uc002mpr.3. human. [Q9BXR0-1 ]

Organism-specific databases

CTDi 81890.
GeneCardsi GC19P010812.
HGNCi HGNC:23797. QTRT1.
HPAi HPA048651.
HPA049440.
MIMi 609615. gene.
neXtProti NX_Q9BXR0.
PharmGKBi PA134963074.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0343.
GeneTreei ENSGT00530000063679.
HOGENOMi HOG000223473.
HOVERGENi HBG101131.
InParanoidi Q9BXR0.
KOi K00773.
OMAi SSCCAPF.
OrthoDBi EOG7W41BR.
PhylomeDBi Q9BXR0.
TreeFami TF300732.

Enzyme and pathway databases

UniPathwayi UPA00392 .

Miscellaneous databases

ChiTaRSi QTRT1. human.
GenomeRNAii 81890.
NextBioi 35471532.
PROi Q9BXR0.
SOURCEi Search...

Gene expression databases

Bgeei Q9BXR0.
CleanExi HS_QTRT1.
ExpressionAtlasi Q9BXR0. baseline and differential.
Genevestigatori Q9BXR0.

Family and domain databases

Gene3Di 3.20.20.105. 1 hit.
HAMAPi MF_00168. Q_tRNA_Tgt.
InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view ]
PANTHERi PTHR11962. PTHR11962. 1 hit.
Pfami PF01702. TGT. 1 hit.
[Graphical view ]
SUPFAMi SSF51713. SSF51713. 1 hit.
TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit."
    Deshpande K.L., Katze J.R.
    Gene 265:205-212(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Spleen.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Amygdala.
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-403 (ISOFORM 1).
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTGT_HUMAN
AccessioniPrimary (citable) accession number: Q9BXR0
Secondary accession number(s): B4DFM7, Q96BQ4, Q9BXQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: June 16, 2009
Last modified: October 29, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3