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Q9BXR0

- TGT_HUMAN

UniProt

Q9BXR0 - TGT_HUMAN

Protein

Queuine tRNA-ribosyltransferase

Gene

QTRT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 3 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.By similarity

    Catalytic activityi

    Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei106 – 1061SubstrateBy similarity
    Active sitei279 – 2791NucleophileBy similarity
    Metal bindingi317 – 3171ZincBy similarity
    Metal bindingi319 – 3191ZincBy similarity
    Metal bindingi322 – 3221ZincBy similarity
    Metal bindingi348 – 3481ZincBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. queuine tRNA-ribosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB
    2. tRNA modification Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Queuosine biosynthesis, tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Queuine tRNA-ribosyltransferase (EC:2.4.2.29)
    Alternative name(s):
    Guanine insertion enzyme
    tRNA-guanine transglycosylase
    Gene namesi
    Name:QTRT1
    Synonyms:TGT, TGUT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:23797. QTRT1.

    Subcellular locationi

    Cytoplasm By similarity. Mitochondrion By similarity. Nucleus By similarity
    Note: Weakly associates with mitochondria, possibly via QTRTD1.By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134963074.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 403402Queuine tRNA-ribosyltransferasePRO_0000135565Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9BXR0.
    PaxDbiQ9BXR0.
    PRIDEiQ9BXR0.

    PTM databases

    PhosphoSiteiQ9BXR0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9BXR0.
    BgeeiQ9BXR0.
    CleanExiHS_QTRT1.
    GenevestigatoriQ9BXR0.

    Organism-specific databases

    HPAiHPA048651.
    HPA049440.

    Interactioni

    Subunit structurei

    Interacts with QTRTD1.By similarity

    Protein-protein interaction databases

    BioGridi123623. 9 interactions.
    IntActiQ9BXR0. 7 interactions.
    MINTiMINT-4656737.
    STRINGi9606.ENSP00000250237.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BXR0.
    SMRiQ9BXR0. Positions 27-376.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0343.
    HOGENOMiHOG000223473.
    HOVERGENiHBG101131.
    InParanoidiQ9BXR0.
    KOiK00773.
    OMAiSSCCAPF.
    OrthoDBiEOG7W41BR.
    PhylomeDBiQ9BXR0.
    TreeFamiTF300732.

    Family and domain databases

    Gene3Di3.20.20.105. 1 hit.
    HAMAPiMF_00168. Q_tRNA_Tgt.
    InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view]
    PANTHERiPTHR11962. PTHR11962. 1 hit.
    PfamiPF01702. TGT. 1 hit.
    [Graphical view]
    SUPFAMiSSF51713. SSF51713. 1 hit.
    TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9BXR0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGAATQASL ESAPRIMRLV AECSRSRARA GELWLPHGTV ATPVFMPVGT    50
    QATMKGITTE QLDALGCRIC LGNTYHLGLR PGPELIQKAN GLHGFMNWPH 100
    NLLTDSGGFQ MVSLVSLSEV TEEGVRFRSP YDGNETLLSP EKSVQIQNAL 150
    GSDIIMQLDD VVSSTVTGPR VEEAMYRSIR WLDRCIAAHQ RPDKQNLFAI 200
    IQGGLDADLR ATCLEEMTKR DVPGFAIGGL SGGESKSQFW RMVALSTSRL 250
    PKDKPRYLMG VGYATDLVVC VALGCDMFDC VFPTRTARFG SALVPTGNLQ 300
    LRKKVFEKDF GPIDPECTCP TCQKHSRAFL HALLHSDNTA ALHHLTVHNI 350
    AYQLQLMSAV RTSIVEKRFP DFVRDFMGAM YGDPTLCPTW ATDALASVGI 400
    TLG 403
    Length:403
    Mass (Da):44,048
    Last modified:June 16, 2009 - v3
    Checksum:i1E30F3C7BF47A106
    GO
    Isoform 2 (identifier: Q9BXR0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         178-225: SIRWLDRCIA...EMTKRDVPGF → PLGLVTGALL...AGRRSPGHLP
         226-403: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:225
    Mass (Da):24,015
    Checksum:iC62B238F305E24A9
    GO

    Sequence cautioni

    The sequence AAH15350.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei178 – 22548SIRWL…DVPGF → PLGLVTGALLQVNPLAGPVH CSPSAAGQAEPLRHYPGWAG RRSPGHLP in isoform 2. 1 PublicationVSP_056470Add
    BLAST
    Alternative sequencei226 – 403178Missing in isoform 2. 1 PublicationVSP_056471Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302783 mRNA. Translation: AAG60033.1.
    AF302784 mRNA. Translation: AAG60034.1.
    AK294166 mRNA. Translation: BAG57488.1.
    AC011475 Genomic DNA. No translation available.
    BC015350 mRNA. Translation: AAH15350.1. Different initiation.
    CCDSiCCDS12248.1.
    RefSeqiNP_112486.1. NM_031209.2.
    UniGeneiHs.631638.

    Genome annotation databases

    EnsembliENST00000250237; ENSP00000250237; ENSG00000213339.
    ENST00000421333; ENSP00000389126; ENSG00000213339.
    GeneIDi81890.
    KEGGihsa:81890.
    UCSCiuc002mpr.3. human.

    Polymorphism databases

    DMDMi239938842.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302783 mRNA. Translation: AAG60033.1 .
    AF302784 mRNA. Translation: AAG60034.1 .
    AK294166 mRNA. Translation: BAG57488.1 .
    AC011475 Genomic DNA. No translation available.
    BC015350 mRNA. Translation: AAH15350.1 . Different initiation.
    CCDSi CCDS12248.1.
    RefSeqi NP_112486.1. NM_031209.2.
    UniGenei Hs.631638.

    3D structure databases

    ProteinModelPortali Q9BXR0.
    SMRi Q9BXR0. Positions 27-376.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123623. 9 interactions.
    IntActi Q9BXR0. 7 interactions.
    MINTi MINT-4656737.
    STRINGi 9606.ENSP00000250237.

    Chemistry

    BindingDBi Q9BXR0.
    ChEMBLi CHEMBL6004.

    PTM databases

    PhosphoSitei Q9BXR0.

    Polymorphism databases

    DMDMi 239938842.

    Proteomic databases

    MaxQBi Q9BXR0.
    PaxDbi Q9BXR0.
    PRIDEi Q9BXR0.

    Protocols and materials databases

    DNASUi 81890.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000250237 ; ENSP00000250237 ; ENSG00000213339 .
    ENST00000421333 ; ENSP00000389126 ; ENSG00000213339 .
    GeneIDi 81890.
    KEGGi hsa:81890.
    UCSCi uc002mpr.3. human.

    Organism-specific databases

    CTDi 81890.
    GeneCardsi GC19P010812.
    HGNCi HGNC:23797. QTRT1.
    HPAi HPA048651.
    HPA049440.
    MIMi 609615. gene.
    neXtProti NX_Q9BXR0.
    PharmGKBi PA134963074.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0343.
    HOGENOMi HOG000223473.
    HOVERGENi HBG101131.
    InParanoidi Q9BXR0.
    KOi K00773.
    OMAi SSCCAPF.
    OrthoDBi EOG7W41BR.
    PhylomeDBi Q9BXR0.
    TreeFami TF300732.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .

    Miscellaneous databases

    ChiTaRSi QTRT1. human.
    GenomeRNAii 81890.
    NextBioi 72242.
    PROi Q9BXR0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BXR0.
    Bgeei Q9BXR0.
    CleanExi HS_QTRT1.
    Genevestigatori Q9BXR0.

    Family and domain databases

    Gene3Di 3.20.20.105. 1 hit.
    HAMAPi MF_00168. Q_tRNA_Tgt.
    InterProi IPR004803. Queuine_tRNA-ribosylTrfase.
    IPR002616. tRNA_ribo_trans-like.
    [Graphical view ]
    PANTHERi PTHR11962. PTHR11962. 1 hit.
    Pfami PF01702. TGT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51713. SSF51713. 1 hit.
    TIGRFAMsi TIGR00430. Q_tRNA_tgt. 1 hit.
    TIGR00449. tgt_general. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit."
      Deshpande K.L., Katze J.R.
      Gene 265:205-212(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Spleen.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Amygdala.
    3. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-403 (ISOFORM 1).
      Tissue: Brain.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTGT_HUMAN
    AccessioniPrimary (citable) accession number: Q9BXR0
    Secondary accession number(s): B4DFM7, Q96BQ4, Q9BXQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2002
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 118 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3