Q9BXL7 (CAR11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caspase recruitment domain-containing protein 11

Alternative name(s):
CARD-containing MAGUK protein 1
Short name=Carma 1

Gene names

Name:	CARD11
Synonyms:	CARMA1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1154 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10.
Subunit structure	Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. CARD11 and BCL10 bind to each other by CARD-CARD interaction. Interacts (via PDZ domain) with DPP4 (via cytoplasmic tail). Ref.7
Subcellular location	Cytoplasm. Membrane raft. Note: Colocalized with DPP4 in membrane rafts. Ref.7
Tissue specificity	Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLaS3, MOLT-4, A-549 and G431 cells. Ref.1
Post-translational modification	Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCB and PRKCQ leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling By similarity.
Sequence similarities	Contains 1 CARD domain. Contains 1 guanylate kinase-like domain. Contains 1 PDZ (DHR) domain.
Caution	Supposed to contain a SH3 domain which is not detected by PROSITE, Pfam or SMART.
Sequence caution	The sequence AAG53402.1 differs from that shown. Reason: Erroneous initiation. The sequence AAI11720.2 differs from that shown. Reason: Erroneous initiation. The sequence AAQ96893.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAL23962.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell costimulation Inferred from direct assay Ref.7. Source: UniProtKB T cell receptor signaling pathway Traceable author statement. Source: Reactome positive regulation of B cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara positive regulation of NF-kappaB transcription factor activity Inferred from mutant phenotype PubMed 12154360. Source: UniProtKB positive regulation of T cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of cytokine production Inferred from mutant phenotype PubMed 12154360. Source: UniProtKB positive regulation of interleukin-2 biosynthetic process Inferred from electronic annotation. Source: Compara regulation of B cell differentiation Inferred from electronic annotation. Source: Compara regulation of T cell differentiation Inferred from electronic annotation. Source: Compara regulation of apoptotic process Inferred from electronic annotation. Source: InterPro thymic T cell selection Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Traceable author statement. Source: Reactome membrane raft Inferred from direct assay PubMed 12154360Ref.7. Source: UniProtKB plasma membrane Inferred from direct assay PubMed 12154360. Source: UniProtKB
Molecular_function	guanylate kinase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1154	1154	Caspase recruitment domain-containing protein 11		PRO_0000144086
Regions
	Domain	18 – 110	93	CARD
	Domain	667 – 755	89	PDZ
	Domain	973 – 1140	168	Guanylate kinase-like
	Coiled coil	130 – 449	320	Potential
Amino acid modifications
	Modified residue	466	1	Phosphoserine Ref.8
	Modified residue	559	1	Phosphoserine; by PKC/PRKCB and PKC/PRKCQ By similarity
	Modified residue	593	1	Phosphoserine Ref.8
	Modified residue	644	1	Phosphoserine; by PKC/PRKCB and PKC/PRKCQ By similarity
	Modified residue	652	1	Phosphoserine; by PKC/PRKCB and PKC/PRKCQ By similarity
	Modified residue	925	1	Phosphoserine Ref.8
Natural variations
	Natural variant	670	1	T → M. Corresponds to variant rs3735134 [ dbSNP | Ensembl ].		VAR_028117
	Natural variant	694	1	S → L. Corresponds to variant rs3735133 [ dbSNP | Ensembl ].		VAR_028118
Experimental info
	Sequence conflict	815	1	L → P in AAG53402. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9BXL7 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: 2F3512D903795D18	FASTA	1,154	133,284
	10 20 30 40 50 60 MPGGGPEMDD YMETLKDEED ALWENVECNR HMLSRYINPA KLTPYLRQCK VIDEQDEDEV 70 80 90 100 110 120 LNAPMLPSKI NRAGRLLDIL HTKGQRGYVV FLESLEFYYP ELYKLVTGKE PTRRFSTIVV 130 140 150 160 170 180 EEGHEGLTHF LMNEVIKLQQ QMKAKDLQRC ELLARLRQLE DEKKQMTLTR VELLTFQERY 190 200 210 220 230 240 YKMKEERDSY NDELVKVKDD NYNLAMRYAQ LSEEKNMAVM RSRDLQLEID QLKHRLNKME 250 260 270 280 290 300 EECKLERNQS LKLKNDIENR PKKEQVLELE RENEMLKTKN QELQSIIQAG KRSLPDSDKA 310 320 330 340 350 360 ILDILEHDRK EALEDRQELV NRIYNLQEEA RQAEELRDKY LEEKEDLELK CSTLGKDCEM 370 380 390 400 410 420 YKHRMNTVML QLEEVERERD QAFHSRDEAQ TQYSQCLIEK DKYRKQIREL EEKNDEMRIE 430 440 450 460 470 480 MVRREACIVN LESKLRRLSK DSNNLDQSLP RNLPVTIISQ DFGDASPRTN GQEADDSSTS 490 500 510 520 530 540 EESPEDSKYF LPYHPPQRRM NLKGIQLQRA KSPISLKRTS DFQAKGHEEE GTDASPSSCG 550 560 570 580 590 600 SLPITNSFTK MQPPRSRSSI MSITAEPPGN DSIVRRYKED APHRSTVEED NDSGGFDALD 610 620 630 640 650 660 LDDDSHERYS FGPSSIHSSS SSHQSEGLDA YDLEQVNLMF RKFSLERPFR PSVTSVGHVR 670 680 690 700 710 720 GPGPSVQHTT LNGDSLTSQL TLLGGNARGS FVHSVKPGSL AEKAGLREGH QLLLLEGCIR 730 740 750 760 770 780 GERQSVPLDT CTKEEAHWTI QRCSGPVTLH YKVNHEGYRK LVKDMEDGLI TSGDSFYIRL 790 800 810 820 830 840 NLNISSQLDA CTMSLKCDDV VHVRDTMYQD RHEWLCARVD PFTDHDLDMG TIPSYSRAQQ 850 860 870 880 890 900 LLLVKLQRLM HRGSREEVDG THHTLRALRN TLQPEEALST SDPRVSPRLS RASFLFGQLL 910 920 930 940 950 960 QFVSRSENKY KRMNSNERVR IISGSPLGSL ARSSLDATKL LTEKQEELDP ESELGKNLSL 970 980 990 1000 1010 1020 IPYSLVRAFY CERRRPVLFT PTVLAKTLVQ RLLNSGGAME FTICKSDIVT RDEFLRRQKT 1030 1040 1050 1060 1070 1080 ETIIYSREKN PNAFECIAPA NIEAVAAKNK HCLLEAGIGC TRDLIKSNIY PIVLFIRVCE 1090 1100 1110 1120 1130 1140 KNIKRFRKLL PRPETEEEFL RVCRLKEKEL EALPCLYATV EPDMWGSVEE LLRVVKDKIG 1150 EEQRKTIWVD EDQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with Bcl10 and activate NF-kappaB." Bertin J., Wang L., Guo Y., Jacobson M.D., Poyet J.-L., Srinivasula S.M., Merriam S., DiStefano P.S., Alnemri E.S. J. Biol. Chem. 276:11877-11882(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation." Gaide O., Martinon F., Micheau O., Bonnet D., Thome M., Tschopp J. FEBS Lett. 496:121-127(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-1154.
[6]	Erratum Gaide O., Martinon F., Micheau O., Bonnet D., Thome M., Tschopp J. FEBS Lett. 505:198-198(2001)
[7]	"Caveolin-1 triggers T-cell activation via CD26 in association with CARMA1." Ohnuma K., Uchiyama M., Yamochi T., Nishibashi K., Hosono O., Takahashi N., Kina S., Tanaka H., Lin X., Dang N.H., Morimoto C. J. Biol. Chem. 282:10117-10131(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A MEMBRANE RAFT COMPLEX, INTERACTION WITH DPP4, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466; SER-593 AND SER-925, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF322641 mRNA. Translation: AAG53402.1. Different initiation. AC004906 Genomic DNA. Translation: AAQ96893.1. Sequence problems. CH236953 Genomic DNA. Translation: EAL23962.1. Sequence problems. BC111719 mRNA. Translation: AAI11720.2. Different initiation. AF352576 mRNA. Translation: AAL34460.1.
IPI	IPI00869011.
RefSeq	NP_115791.3. NM_032415.4.
UniGene	Hs.648101.
3D structure databases
ProteinModelPortal	Q9BXL7.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-41797N.
MINT	MINT-193901.
STRING	9606.ENSP00000380150.
PTM databases
PhosphoSite	Q9BXL7.
Polymorphism databases
DMDM	172046231.
Proteomic databases
PaxDb	Q9BXL7.
PRIDE	Q9BXL7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000396946; ENSP00000380150; ENSG00000198286.
GeneID	84433.
KEGG	hsa:84433.
UCSC	uc003smv.3. human.
Organism-specific databases
CTD	84433.
GeneCards	GC07M002912.
H-InvDB	HIX0033921.
HGNC	HGNC:16393. CARD11.
HPA	HPA052984.
MIM	607210. gene.
neXtProt	NX_Q9BXL7.
PharmGKB	PA26073.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG47732.
HOVERGEN	HBG099790.
InParanoid	Q9BXL7.
KO	K07367.
OMA	AKNKHCL.
OrthoDB	EOG4MW85G.
Enzyme and pathway databases
Pathway_Interaction_DB	bcr_5pathway. BCR signaling pathway. tcrpathway. TCR signaling in naive CD4+ T cells. cd8tcrpathway. TCR signaling in naive CD8+ T cells.
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q9BXL7.
Bgee	Q9BXL7.
CleanEx	HS_CARD11.
Genevestigator	Q9BXL7.
GermOnline	ENSG00000198286. Homo sapiens.
Family and domain databases
Gene3D	1.10.533.10. 1 hit.
InterPro	IPR001315. CARD. IPR011029. DEATH-like_dom. IPR001478. PDZ. [Graphical view]
Pfam	PF00619. CARD. 1 hit. [Graphical view]
SMART	SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit. SSF50156. PDZ. 1 hit.
PROSITE	PS50209. CARD. 1 hit. PS00856. GUANYLATE_KINASE_1. False negative. PS50052. GUANYLATE_KINASE_2. False negative. PS50106. PDZ. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CARD11. human.
GenomeRNAi	84433.
NextBio	74170.
SOURCE	Search...

Entry information

Entry name

CAR11_HUMAN

Accession

Primary (citable) accession number: Q9BXL7
Secondary accession number(s): A4D1Z7, Q2NKN7, Q548H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	February 26, 2008
Last modified:	May 1, 2013
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.