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Protein

Hemogen

Gene

HEMGN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Hemogen
Alternative name(s):
Erythroid differentiation-associated gene protein
Short name:
EDAG-1
Hemopoietic gene protein
Negative differentiation regulator protein
Gene namesi
Name:HEMGN
Synonyms:EDAG, NDR
ORF Names:PRO1037, PRO1620
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:17509. HEMGN.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38458.

Polymorphism and mutation databases

DMDMi74752432.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484HemogenPRO_0000245361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei123 – 1231PhosphoserineCombined sources
Modified residuei159 – 1591PhosphoserineBy similarity
Modified residuei181 – 1811PhosphoserineCombined sources
Modified residuei188 – 1881PhosphoserineCombined sources
Modified residuei201 – 2011PhosphoserineCombined sources
Modified residuei246 – 2461PhosphothreonineBy similarity
Modified residuei349 – 3491PhosphoserineCombined sources
Modified residuei353 – 3531PhosphoserineCombined sources
Modified residuei360 – 3601PhosphothreonineCombined sources
Modified residuei363 – 3631PhosphoserineCombined sources
Modified residuei367 – 3671PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9BXL5.
PaxDbiQ9BXL5.
PeptideAtlasiQ9BXL5.
PRIDEiQ9BXL5.

PTM databases

iPTMnetiQ9BXL5.
PhosphoSiteiQ9BXL5.

Expressioni

Tissue specificityi

Expressed in hematopoietic precursor cells, thyroid and spermatids (at protein level). Expressed in bone marrow, testis, thymus. Expressed in prostate cancer and ovarian cancer. Also expressed in thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia.6 Publications

Developmental stagei

Expressed in fetal liver, kidney and brain.2 Publications

Inductioni

Down-regulated during megakaryocytic differentiation of K562 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) (at protein level). Up-regulated in normal PBMCs by mitogens.2 Publications

Gene expression databases

BgeeiQ9BXL5.
CleanExiHS_HEMGN.
ExpressionAtlasiQ9BXL5. baseline and differential.
GenevisibleiQ9BXL5. HS.

Organism-specific databases

HPAiHPA019572.
HPA019604.
HPA019606.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CTBP1Q133632EBI-3916399,EBI-908846
NPM1P067487EBI-3916399,EBI-78579

Protein-protein interaction databases

BioGridi120641. 17 interactions.
IntActiQ9BXL5. 32 interactions.
MINTiMINT-7220237.
STRINGi9606.ENSP00000259456.

Structurei

3D structure databases

ProteinModelPortaliQ9BXL5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 8781Necessary for nuclear localizationAdd
BLAST

Phylogenomic databases

eggNOGiENOG410IXJU. Eukaryota.
ENOG411096P. LUCA.
GeneTreeiENSGT00390000004522.
HOGENOMiHOG000061736.
HOVERGENiHBG080273.
InParanoidiQ9BXL5.
OMAiPEIYQET.
OrthoDBiEOG7K3TN9.
PhylomeDBiQ9BXL5.
TreeFamiTF338654.

Family and domain databases

InterProiIPR033272. Hemogen.
[Graphical view]
PANTHERiPTHR15993:SF6. PTHR15993:SF6. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BXL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLGKDQSHL KHHQTPDPHQ EENHSPEVIG TWSLRNRELL RKRKAEVHEK
60 70 80 90 100
ETSQWLFGEQ KKRKQQRTGK GNRRGRKRQQ NTELKVEPQP QIEKEIVEKA
110 120 130 140 150
LAPIEKKTEP PGSITKVFPS VASPQKVVPE EHFSEICQES NIYQENFSEY
160 170 180 190 200
QEIAVQNHSS ETCQHVSEPE DLSPKMYQEI SVLQDNSSKI CQDMKEPEDN
210 220 230 240 250
SPNTCQVISV IQDHPFKMYQ DMAKREDLAP KMCQEAAVPK ILPCPTSEDT
260 270 280 290 300
ADLAGCSLQA YPKPDVPKGY ILDTDQNPAE PEEYNETDQG IAETEGLFPK
310 320 330 340 350
IQEIAEPKDL STKTHQESAE PKYLPHKTCN EIIVPKAPSH KTIQETPHSE
360 370 380 390 400
DYSIEINQET PGSEKYSPET YQEIPGLEEY SPEIYQETSQ LEEYSPEIYQ
410 420 430 440 450
ETPGPEDLST ETYKNKDVPK ECFPEPHQET GGPQGQDPKA HQEDAKDAYT
460 470 480
FPQEMKEKPK EEPGIPAILN ESHPENDVYS YVLF
Length:484
Mass (Da):55,341
Last modified:June 1, 2001 - v1
Checksum:iE1464D1C7BEA1F07
GO

Sequence cautioni

The sequence AAF67133.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAF71041.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAG35488.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781R → K in AAP75762 (PubMed:14648837).Curated
Sequence conflicti78 – 781R → K in AAF67133 (PubMed:15332117).Curated
Sequence conflicti96 – 972IV → M in AAH48324 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322875 mRNA. Translation: AAK26295.1.
AY244805 mRNA. Translation: AAP75762.1.
AY255672 mRNA. Translation: AAP81221.1.
AF228713 mRNA. Translation: AAF67133.1. Different initiation.
AL499604 Genomic DNA. Translation: CAI12808.1.
BC048324 mRNA. Translation: AAH48324.1.
AF130060 mRNA. Translation: AAG35488.1. Different initiation.
AF116617 mRNA. Translation: AAF71041.1. Different initiation.
CCDSiCCDS6731.1.
RefSeqiNP_060907.2. NM_018437.4.
NP_932095.1. NM_197978.2.
XP_005252143.1. XM_005252086.1.
XP_011517147.1. XM_011518845.1.
XP_011517148.1. XM_011518846.1.
UniGeneiHs.176626.

Genome annotation databases

EnsembliENST00000259456; ENSP00000259456; ENSG00000136929.
ENST00000616898; ENSP00000480020; ENSG00000136929.
GeneIDi55363.
KEGGihsa:55363.
UCSCiuc004axy.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322875 mRNA. Translation: AAK26295.1.
AY244805 mRNA. Translation: AAP75762.1.
AY255672 mRNA. Translation: AAP81221.1.
AF228713 mRNA. Translation: AAF67133.1. Different initiation.
AL499604 Genomic DNA. Translation: CAI12808.1.
BC048324 mRNA. Translation: AAH48324.1.
AF130060 mRNA. Translation: AAG35488.1. Different initiation.
AF116617 mRNA. Translation: AAF71041.1. Different initiation.
CCDSiCCDS6731.1.
RefSeqiNP_060907.2. NM_018437.4.
NP_932095.1. NM_197978.2.
XP_005252143.1. XM_005252086.1.
XP_011517147.1. XM_011518845.1.
XP_011517148.1. XM_011518846.1.
UniGeneiHs.176626.

3D structure databases

ProteinModelPortaliQ9BXL5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120641. 17 interactions.
IntActiQ9BXL5. 32 interactions.
MINTiMINT-7220237.
STRINGi9606.ENSP00000259456.

PTM databases

iPTMnetiQ9BXL5.
PhosphoSiteiQ9BXL5.

Polymorphism and mutation databases

DMDMi74752432.

Proteomic databases

MaxQBiQ9BXL5.
PaxDbiQ9BXL5.
PeptideAtlasiQ9BXL5.
PRIDEiQ9BXL5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000259456; ENSP00000259456; ENSG00000136929.
ENST00000616898; ENSP00000480020; ENSG00000136929.
GeneIDi55363.
KEGGihsa:55363.
UCSCiuc004axy.4. human.

Organism-specific databases

CTDi55363.
GeneCardsiHEMGN.
HGNCiHGNC:17509. HEMGN.
HPAiHPA019572.
HPA019604.
HPA019606.
MIMi610715. gene.
neXtProtiNX_Q9BXL5.
PharmGKBiPA38458.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IXJU. Eukaryota.
ENOG411096P. LUCA.
GeneTreeiENSGT00390000004522.
HOGENOMiHOG000061736.
HOVERGENiHBG080273.
InParanoidiQ9BXL5.
OMAiPEIYQET.
OrthoDBiEOG7K3TN9.
PhylomeDBiQ9BXL5.
TreeFamiTF338654.

Miscellaneous databases

ChiTaRSiHEMGN. human.
GeneWikiiHEMGN.
GenomeRNAii55363.
PROiQ9BXL5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BXL5.
CleanExiHS_HEMGN.
ExpressionAtlasiQ9BXL5. baseline and differential.
GenevisibleiQ9BXL5. HS.

Family and domain databases

InterProiIPR033272. Hemogen.
[Graphical view]
PANTHERiPTHR15993:SF6. PTHR15993:SF6. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Hemogen is a novel nuclear factor specifically expressed in mouse hematopoietic development and its human homologue EDAG maps to chromosome 9q22, a region containing breakpoints of hematological neoplasms."
    Yang L.V., Nicholson R.H., Kaplan J., Galy A., Li L.
    Mech. Dev. 104:105-111(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Bone marrow.
  2. "Alternative promoters and polyadenylation regulate tissue-specific expression of Hemogen isoforms during hematopoiesis and spermatogenesis."
    Yang L.V., Heng H.H., Wan J., Southwood C.M., Gow A., Li L.
    Dev. Dyn. 228:606-616(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  3. "EDAG regulates the proliferation and differentiation of hematopoietic cells and resists cell apoptosis through the activation of nuclear factor-kappa B."
    Li C.Y., Zhan Y.Q., Xu C.W., Xu W.X., Wang S.Y., Lv J., Zhou Y., Yue P.B., Chen B., Yang X.M.
    Cell Death Differ. 11:1299-1308(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
  4. "Down-regulation of human NDR gene in megakaryocytic differentiation of erythroleukemia K562 cells."
    Liu C.C., Chou Y.L., Ch'ang L.Y.
    J. Biomed. Sci. 11:104-116(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, INDUCTION.
  5. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  7. "Functional prediction of the coding sequences of 75 new genes deduced by analysis of cDNA clones from human fetal liver."
    Zhang C., Yu Y., Zhang S., Wei H., Bi J., Zhou G., Dong C., Zai Y., Xu W., Gao F., Liu M., He F.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-484.
    Tissue: Fetal liver.
  8. "Functional prediction of the coding sequences of 121 new genes deduced by analysis of cDNA clones from human fetal liver."
    Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Ouyang S., Luo L., Bi J., Liu M., He F.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 115-484.
    Tissue: Fetal liver.
  9. "High expression of EDAG and its significance in AML."
    An L.-L., Li G., Wu K.-F., Ma X.-T., Zheng G.-G., Qiu L.-G., Song Y.-H.
    Leukemia 19:1499-1502(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123; SER-181; SER-188; SER-201; SER-349; SER-353; THR-360 AND SER-363, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Erythroleukemia.
  12. "Scanning of novel cancer/testis proteins by human testis proteomic analysis."
    Liu M., Hu Z., Qi L., Wang J., Zhou T., Guo Y., Zeng Y., Zheng B., Wu Y., Zhang P., Chen X., Tu W., Zhang T., Zhou Q., Jiang M., Guo X., Zhou Z., Sha J.
    Proteomics 13:1200-1210(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiHEMGN_HUMAN
AccessioniPrimary (citable) accession number: Q9BXL5
Secondary accession number(s): Q6XAR3, Q86XY5, Q9NPC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.