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Protein

Cytosolic 5'-nucleotidase 1A

Gene

NT5C1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia.1 Publication

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Enzyme regulationi

Activated by ADP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei211 – 2111NucleophileBy similarity

GO - Molecular functioni

  1. 5'-nucleotidase activity Source: UniProtKB
  2. magnesium ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. adenosine metabolic process Source: Ensembl
  2. dephosphorylation Source: GOC
  3. nucleobase-containing small molecule metabolic process Source: Reactome
  4. nucleoside metabolic process Source: UniProtKB
  5. purine nucleobase metabolic process Source: Reactome
  6. purine nucleoside monophosphate catabolic process Source: Ensembl
  7. purine nucleotide catabolic process Source: Reactome
  8. pyrimidine nucleobase metabolic process Source: Reactome
  9. pyrimidine nucleoside catabolic process Source: Reactome
  10. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS04074-MONOMER.
BRENDAi3.1.3.5. 2681.
ReactomeiREACT_1023. Pyrimidine catabolism.
REACT_2086. Purine catabolism.
SABIO-RKQ9BXI3.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic 5'-nucleotidase 1A (EC:3.1.3.5)
Short name:
cN1A
Alternative name(s):
Cytosolic 5'-nucleotidase IA
Short name:
cN-I
Short name:
cN-IA
Gene namesi
Name:NT5C1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:17819. NT5C1A.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31799.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Cytosolic 5'-nucleotidase 1APRO_0000144795Add
BLAST

Proteomic databases

PaxDbiQ9BXI3.
PRIDEiQ9BXI3.

PTM databases

DEPODiQ9BXI3.
PhosphoSiteiQ9BXI3.

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas.1 Publication

Gene expression databases

BgeeiQ9BXI3.
CleanExiHS_NT5C1A.
GenevestigatoriQ9BXI3.

Organism-specific databases

HPAiHPA050283.
HPA054158.

Interactioni

Protein-protein interaction databases

BioGridi124149. 1 interaction.
STRINGi9606.ENSP00000235628.

Structurei

3D structure databases

ProteinModelPortaliQ9BXI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 5'-nucleotidase type 3 family.Curated

Phylogenomic databases

eggNOGiNOG46880.
GeneTreeiENSGT00390000017767.
HOGENOMiHOG000243802.
HOVERGENiHBG101325.
KOiK01081.
OMAiKSPKPQN.
OrthoDBiEOG79KPF1.
PhylomeDBiQ9BXI3.
TreeFamiTF329831.

Family and domain databases

InterProiIPR010394. 5-nucleotidase.
[Graphical view]
PfamiPF06189. 5-nucleotidase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BXI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPGQPREPQ EPREPGPGAE TAAAPVWEEA KIFYDNLAPK KKPKSPKPQN
60 70 80 90 100
AVTIAVSSRA LFRMDEEQQI YTEQGVEEYV RYQLEHENEP FSPGPAFPFV
110 120 130 140 150
KALEAVNRRL RELYPDSEDV FDIVLMTNNH AQVGVRLINS INHYDLFIER
160 170 180 190 200
FCMTGGNSPI CYLKAYHTNL YLSADAEKVR EAIDEGIAAA TIFSPSRDVV
210 220 230 240 250
VSQSQLRVAF DGDAVLFSDE SERIVKAHGL DRFFEHEKAH ENKPLAQGPL
260 270 280 290 300
KGFLEALGRL QKKFYSKGLR LECPIRTYLV TARSAASSGA RALKTLRSWG
310 320 330 340 350
LETDEALFLA GAPKGPLLEK IRPHIFFDDQ MFHVAGAQEM GTVAAHVPYG
360
VAQTPRRTAP AKQAPSAQ
Length:368
Mass (Da):41,021
Last modified:June 1, 2001 - v1
Checksum:i7FB3CCEED50BA50D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 113Missing in AAK30000 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF331801 mRNA. Translation: AAK01294.1.
AY028778 mRNA. Translation: AAK30000.1.
AL035404 Genomic DNA. Translation: CAI19573.1.
BC103879 mRNA. Translation: AAI03880.1.
BC103880 mRNA. Translation: AAI03881.1.
CCDSiCCDS440.1.
RefSeqiNP_115915.1. NM_032526.2.
UniGeneiHs.307006.

Genome annotation databases

EnsembliENST00000235628; ENSP00000235628; ENSG00000116981.
GeneIDi84618.
KEGGihsa:84618.
UCSCiuc001cdq.1. human.

Polymorphism databases

DMDMi47116736.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF331801 mRNA. Translation: AAK01294.1.
AY028778 mRNA. Translation: AAK30000.1.
AL035404 Genomic DNA. Translation: CAI19573.1.
BC103879 mRNA. Translation: AAI03880.1.
BC103880 mRNA. Translation: AAI03881.1.
CCDSiCCDS440.1.
RefSeqiNP_115915.1. NM_032526.2.
UniGeneiHs.307006.

3D structure databases

ProteinModelPortaliQ9BXI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124149. 1 interaction.
STRINGi9606.ENSP00000235628.

PTM databases

DEPODiQ9BXI3.
PhosphoSiteiQ9BXI3.

Polymorphism databases

DMDMi47116736.

Proteomic databases

PaxDbiQ9BXI3.
PRIDEiQ9BXI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000235628; ENSP00000235628; ENSG00000116981.
GeneIDi84618.
KEGGihsa:84618.
UCSCiuc001cdq.1. human.

Organism-specific databases

CTDi84618.
GeneCardsiGC01M040154.
HGNCiHGNC:17819. NT5C1A.
HPAiHPA050283.
HPA054158.
MIMi610525. gene.
neXtProtiNX_Q9BXI3.
PharmGKBiPA31799.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG46880.
GeneTreeiENSGT00390000017767.
HOGENOMiHOG000243802.
HOVERGENiHBG101325.
KOiK01081.
OMAiKSPKPQN.
OrthoDBiEOG79KPF1.
PhylomeDBiQ9BXI3.
TreeFamiTF329831.

Enzyme and pathway databases

BioCyciMetaCyc:HS04074-MONOMER.
BRENDAi3.1.3.5. 2681.
ReactomeiREACT_1023. Pyrimidine catabolism.
REACT_2086. Purine catabolism.
SABIO-RKQ9BXI3.

Miscellaneous databases

GenomeRNAii84618.
NextBioi74492.
PROiQ9BXI3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BXI3.
CleanExiHS_NT5C1A.
GenevestigatoriQ9BXI3.

Family and domain databases

InterProiIPR010394. 5-nucleotidase.
[Graphical view]
PfamiPF06189. 5-nucleotidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human cytosolic 5'-nucleotidase I: characterization and role in nucleoside analog resistance."
    Hunsucker S.A., Spychala J., Mitchell B.S.
    J. Biol. Chem. 276:10498-10504(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Heart.
  2. "5'-nucleotidase I from human heart."
    Lowenstein J.M., Huang J., Steiner A.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry namei5NT1A_HUMAN
AccessioniPrimary (citable) accession number: Q9BXI3
Secondary accession number(s): Q3SYB9, Q5TG98, Q9BWT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2001
Last modified: March 4, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.