Q9BXI3 (5NT1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic 5'-nucleotidase 1A
Short name=cN1A
EC=3.1.3.5

Alternative name(s):
Cytosolic 5'-nucleotidase IA
Short name=cN-I
Short name=cN-IA

Gene names

Name:

NT5C1A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia. Ref.1
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Magnesium.
Enzyme regulation	Activated by ADP.
Subcellular location	Cytoplasm.
Tissue specificity	Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas. Ref.1
Sequence similarities	Belongs to the 5'-nucleotidase type 3 family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine base metabolic process Traceable author statement. Source: Reactome purine nucleotide catabolic process Traceable author statement. Source: Reactome pyrimidine base metabolic process Traceable author statement. Source: Reactome pyrimidine nucleoside catabolic process Traceable author statement. Source: Reactome
Cellular component	cytosol Non-traceable author statement Ref.1. Source: UniProtKB
Molecular function	5'-nucleotidase activity Non-traceable author statement Ref.1. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Cytosolic 5'-nucleotidase 1A		PRO_0000144795
Sites
	Active site	211	1	Nucleophile By similarity
Experimental info
	Sequence conflict	9 – 11	3	Missing in AAK30000. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9BXI3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7FB3CCEED50BA50D	FASTA	368	41,021
	10 20 30 40 50 60 MEPGQPREPQ EPREPGPGAE TAAAPVWEEA KIFYDNLAPK KKPKSPKPQN AVTIAVSSRA 70 80 90 100 110 120 LFRMDEEQQI YTEQGVEEYV RYQLEHENEP FSPGPAFPFV KALEAVNRRL RELYPDSEDV 130 140 150 160 170 180 FDIVLMTNNH AQVGVRLINS INHYDLFIER FCMTGGNSPI CYLKAYHTNL YLSADAEKVR 190 200 210 220 230 240 EAIDEGIAAA TIFSPSRDVV VSQSQLRVAF DGDAVLFSDE SERIVKAHGL DRFFEHEKAH 250 260 270 280 290 300 ENKPLAQGPL KGFLEALGRL QKKFYSKGLR LECPIRTYLV TARSAASSGA RALKTLRSWG 310 320 330 340 350 360 LETDEALFLA GAPKGPLLEK IRPHIFFDDQ MFHVAGAQEM GTVAAHVPYG VAQTPRRTAP AKQAPSAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human cytosolic 5'-nucleotidase I: characterization and role in nucleoside analog resistance." Hunsucker S.A., Spychala J., Mitchell B.S. J. Biol. Chem. 276:10498-10504(2001) [PubMed: 11133996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Heart.
[2]	"5'-nucleotidase I from human heart." Lowenstein J.M., Huang J., Steiner A. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF331801 mRNA. Translation: AAK01294.1. AY028778 mRNA. Translation: AAK30000.1. AL035404 Genomic DNA. Translation: CAI19573.1. BC103879 mRNA. Translation: AAI03880.1. BC103880 mRNA. Translation: AAI03881.1.
IPI	IPI00000146.
RefSeq	NP_115915.1. NM_032526.1.
UniGene	Hs.307006.
3D structure databases
ProteinModelPortal	Q9BXI3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9BXI3.
PTM databases
PhosphoSite	Q9BXI3.
Polymorphism databases
DMDM	47116736.
Proteomic databases
PRIDE	Q9BXI3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000235628; ENSP00000235628; ENSG00000116981.
GeneID	84618.
KEGG	hsa:84618.
UCSC	uc001cdq.1. human.
Organism-specific databases
CTD	84618.
GeneCards	GC01M040154.
H-InvDB	HIX0200031.
HGNC	HGNC:17819. NT5C1A.
MIM	610525. gene.
neXtProt	NX_Q9BXI3.
PharmGKB	PA31799.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10998.
GeneTree	ENSGT00390000017767.
HOGENOM	HBG350968.
HOVERGEN	HBG101325.
InParanoid	Q9BXI3.
OMA	LEHENEP.
OrthoDB	EOG4PNXHC.
PhylomeDB	Q9BXI3.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15168.
BRENDA	3.1.3.5. 2681.
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q9BXI3.
Bgee	Q9BXI3.
CleanEx	HS_NT5C1A.
Genevestigator	Q9BXI3.
GermOnline	ENSG00000116981. Homo sapiens.
Family and domain databases
InterPro	IPR010394. 5-nucleotidase. [Graphical view]
KO	K01081.
Pfam	PF06189. 5-nucleotidase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	74492.
SOURCE	Search...

Entry information

Entry name

5NT1A_HUMAN

Accession

Primary (citable) accession number: Q9BXI3
Secondary accession number(s): Q3SYB9, Q5TG98, Q9BWT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.