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Protein

Spermatogenic leucine zipper protein 1

Gene

SPZ1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the DNA sequence 5'-CANNTG-3'(E box) and the G-box motif. May play an important role in the regulation of cell proliferation and differentiation during spermatogenesis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ9BXG8.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatogenic leucine zipper protein 1
Alternative name(s):
Testis-specific protein 1
Testis-specific protein NYD-TSP1
Gene namesi
Name:SPZ1
Synonyms:TSP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:30721. SPZ1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670869.

Polymorphism and mutation databases

BioMutaiSPZ1.
DMDMi134035034.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 430430Spermatogenic leucine zipper protein 1PRO_0000280507Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei107 – 1071PhosphoserineBy similarity
Modified residuei258 – 2581PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by MAPK1/ERK2 and MAPK3/ERK1.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BXG8.
PaxDbiQ9BXG8.
PRIDEiQ9BXG8.

PTM databases

iPTMnetiQ9BXG8.
PhosphoSiteiQ9BXG8.

Expressioni

Tissue specificityi

Specifically and strongly expressed in the testis. Expressed in several tumor cell lines.2 Publications

Gene expression databases

BgeeiQ9BXG8.
CleanExiHS_SPZ1.
ExpressionAtlasiQ9BXG8. baseline and differential.
GenevisibleiQ9BXG8. HS.

Organism-specific databases

HPAiHPA037798.

Interactioni

Subunit structurei

Interacts with PPP1CC isoform gamma-2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
DTNBO609413EBI-8483734,EBI-740402

Protein-protein interaction databases

BioGridi124175. 10 interactions.
IntActiQ9BXG8. 2 interactions.
MINTiMINT-8417723.
STRINGi9606.ENSP00000369611.

Structurei

3D structure databases

ProteinModelPortaliQ9BXG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 17712Helix-loop-helix motifSequence analysisAdd
BLAST
Regioni178 – 24467Basic motifSequence analysisAdd
BLAST
Regioni303 – 32422Leucine-zipperAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili62 – 10241Sequence analysisAdd
BLAST
Coiled coili214 – 26956Sequence analysisAdd
BLAST
Coiled coili316 – 35136Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IVK6. Eukaryota.
ENOG411141P. LUCA.
GeneTreeiENSGT00390000003379.
HOGENOMiHOG000154405.
HOVERGENiHBG093995.
InParanoidiQ9BXG8.
KOiK17482.
OMAiLENECQI.
PhylomeDBiQ9BXG8.
TreeFamiTF337798.

Family and domain databases

InterProiIPR029780. SPZ1.
[Graphical view]
PANTHERiPTHR23158:SF40. PTHR23158:SF40. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9BXG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSAKSAEM PTISKTVNPT PDPHQEYLDP RITIALFEIG SHSPSSWGSL
60 70 80 90 100
PFLKNSSHQV TEQQTAQKFN NLLKEIKDIL KNMAGFEEKI TEAKELFEET
110 120 130 140 150
NITEDVSAHK ENIRGLDKIN EMLSTNLPVS LAPEKEDNEK KQEMILETNI
160 170 180 190 200
TEDVSAHKEN IRGLDKINEM LSTNLPVSLA PEKEDNEKKQ QMIMENQNSE
210 220 230 240 250
NTAQVFARDL VNRLEEKKVL NETQQSQEKA KNRLNVQEET MKIRNNMEQL
260 270 280 290 300
LQEAEHWSKQ HTELSKLIKS YQKSQKDISE TLGNNGVGFQ TQPNNEVSAK
310 320 330 340 350
HELEEQVKKL SHDTYSLQLM AALLENECQI LQQRVEILKE LHHQKQGTLQ
360 370 380 390 400
EKPIQINYKQ DKKNQKPSEA KKVEMYKQNK QAMKGTFWKK DRSCRSLDVC
410 420 430
LNKKACNTQF NIHVARKALR GKMRSASSLR
Length:430
Mass (Da):49,445
Last modified:March 20, 2007 - v2
Checksum:i9B2CD2B5C8F5FB43
GO

Sequence cautioni

The sequence BAC05340.1 differs from that shown.Probable cloning artifact.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301P → S in AAK17995 (PubMed:12778315).Curated
Sequence conflicti301 – 3011H → P in AAK17995 (PubMed:12778315).Curated
Sequence conflicti380 – 3801K → N in BAC05340 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171V → L.4 Publications
Corresponds to variant rs1862136 [ dbSNP | Ensembl ].
VAR_031160
Natural varianti302 – 3021E → K.
Corresponds to variant rs6867419 [ dbSNP | Ensembl ].
VAR_031161

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF333098 mRNA. Translation: AAK17995.1.
AK098575 mRNA. Translation: BAC05340.1. Sequence problems.
AK314007 mRNA. Translation: BAG36718.1.
AC026410 Genomic DNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95848.1.
BC033798 mRNA. Translation: AAH33798.1.
CCDSiCCDS43336.1.
RefSeqiNP_115956.3. NM_032567.3.
UniGeneiHs.519403.

Genome annotation databases

EnsembliENST00000296739; ENSP00000369611; ENSG00000164299.
GeneIDi84654.
KEGGihsa:84654.
UCSCiuc003kgn.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF333098 mRNA. Translation: AAK17995.1.
AK098575 mRNA. Translation: BAC05340.1. Sequence problems.
AK314007 mRNA. Translation: BAG36718.1.
AC026410 Genomic DNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95848.1.
BC033798 mRNA. Translation: AAH33798.1.
CCDSiCCDS43336.1.
RefSeqiNP_115956.3. NM_032567.3.
UniGeneiHs.519403.

3D structure databases

ProteinModelPortaliQ9BXG8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124175. 10 interactions.
IntActiQ9BXG8. 2 interactions.
MINTiMINT-8417723.
STRINGi9606.ENSP00000369611.

PTM databases

iPTMnetiQ9BXG8.
PhosphoSiteiQ9BXG8.

Polymorphism and mutation databases

BioMutaiSPZ1.
DMDMi134035034.

Proteomic databases

EPDiQ9BXG8.
PaxDbiQ9BXG8.
PRIDEiQ9BXG8.

Protocols and materials databases

DNASUi84654.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296739; ENSP00000369611; ENSG00000164299.
GeneIDi84654.
KEGGihsa:84654.
UCSCiuc003kgn.4. human.

Organism-specific databases

CTDi84654.
GeneCardsiSPZ1.
H-InvDBHIX0031998.
HGNCiHGNC:30721. SPZ1.
HPAiHPA037798.
neXtProtiNX_Q9BXG8.
PharmGKBiPA142670869.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IVK6. Eukaryota.
ENOG411141P. LUCA.
GeneTreeiENSGT00390000003379.
HOGENOMiHOG000154405.
HOVERGENiHBG093995.
InParanoidiQ9BXG8.
KOiK17482.
OMAiLENECQI.
PhylomeDBiQ9BXG8.
TreeFamiTF337798.

Enzyme and pathway databases

SignaLinkiQ9BXG8.

Miscellaneous databases

GeneWikiiSPZ1.
GenomeRNAii84654.
NextBioi74614.
PROiQ9BXG8.

Gene expression databases

BgeeiQ9BXG8.
CleanExiHS_SPZ1.
ExpressionAtlasiQ9BXG8. baseline and differential.
GenevisibleiQ9BXG8. HS.

Family and domain databases

InterProiIPR029780. SPZ1.
[Graphical view]
PANTHERiPTHR23158:SF40. PTHR23158:SF40. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of a novel bHLH-Zip gene in human testis."
    Sha J.-H., Zhou Z.-M., Li J.-M., Lin M., Zhu H., Zhu H., Zhou Y.-D., Wang L.-L., Wang Y.-Q., Zhou K.-Y.
    Asian J. Androl. 5:83-88(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT LEU-17.
    Tissue: Testis.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-17.
    Tissue: Testis.
  3. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LEU-17.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-17.
    Tissue: Brain.
  6. "bHLH-zip transcription factor Spz1 mediates mitogen-activated protein kinase cell proliferation, transformation, and tumorigenesis."
    Hsu S.-H., Hsieh-Li H.-M., Huang H.-Y., Huang P.-H., Li H.
    Cancer Res. 65:4041-4050(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiSPZ1_HUMAN
AccessioniPrimary (citable) accession number: Q9BXG8
Secondary accession number(s): B2RA21, Q8N4P1, Q8N7E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: March 16, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The helix-loop-helix and basic motifs form a SPZ1 specific bHLH different from the classical one.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.