Q9BXF6 (RFIP5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rab11 family-interacting protein 5
Short name=Rab11-FIP5

Alternative name(s):
Gamma-SNAP-associated factor 1
Short name=Gaf-1
Phosphoprotein pp75
Rab11-interacting protein Rip11

Gene names

Name:	RAB11FIP5
Synonyms:	GAF1, KIAA0857, RIP11

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Ref.1
Subunit structure	Forms an heterooligomeric complex with RAB11FIP4. Binds NAPG and TROVE2. Binds RAB11A that has been activated by GTP binding. Ref.6
Subcellular location	Cytoplasm. Recycling endosome membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein Ref.1 Ref.5.
Tissue specificity	Detected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas. Ref.4 Ref.5
Domain	Binds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg2+ rather than Ca2+.
Post-translational modification	Phosphorylated on serine and threonine residues. Ref.4 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11
Miscellaneous	Antibodies against RIP11 are found in sera from patients with autoimmune diseases such as systemic lupus erythematosus (SLE) or Sjoegren syndrome (SS). It is also found in the sera from mothers of children with neonatal lupus erythematosus (NLE).
Sequence similarities	Contains 1 C2 domain. Contains 1 FIP-RBD domain.
Sequence caution	The sequence BAA74880.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome Membrane Mitochondrion
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	centrosome Inferred from direct assay. Source: HPA mitochondrial outer membrane Inferred from direct assay Ref.5. Source: UniProtKB recycling endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	gamma-tubulin binding Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 653	653	Rab11 family-interacting protein 5		PRO_0000097307
Regions
	Domain	6 – 128	123	C2
	Domain	586 – 648	63	FIP-RBD
Amino acid modifications
	Modified residue	174	1	Phosphoserine Ref.7
	Modified residue	188	1	Phosphoserine Ref.8
	Modified residue	306	1	Phosphotyrosine By similarity
	Modified residue	307	1	Phosphoserine Ref.8 Ref.9 Ref.10 Ref.11
	Modified residue	359	1	Phosphoserine Ref.10
	Modified residue	538	1	Phosphoserine Ref.9
	Modified residue	553	1	Phosphoserine Ref.10
Experimental info
	Sequence conflict	176	1	Missing in AAH35013. Ref.3
	Sequence conflict	503	1	E → G in AAH35013. Ref.3
	Sequence conflict	599	1	H → N in AAH35013. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9BXF6 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 716725537AA9D86E	FASTA	653	70,415
	10 20 30 40 50 60 MALVRGAEPA AGPSRWLPTH VQVTVLRARG LRGKSSGAGS TSDAYTVIQV GREKYSTSVV 70 80 90 100 110 120 EKTHGCPEWR EECSFELPPG ALDGLLRAQE ADAGPAPWAA SSAAACELVL TTMHRSLIGV 130 140 150 160 170 180 DKFLGQATVA LDEVFGAGRA QHTQWYKLHS KPGKKEKERG EIEVTIQFTR NNLSASMFDL 190 200 210 220 230 240 SMKDKPRSPF SKIRDKMKGK KKYDLESASA ILPSSAIEDP DLGSLGKMGK AKGFFLRNKL 250 260 270 280 290 300 RKSSLTQSNT SLGSDSTLSS ASGSLAYQGP GAELLTRSPS RSSWLSTEGG RDSAQSPKLF 310 320 330 340 350 360 THKRTYSDEA NQMRVAPPRA LLDLQGHLDA ASRSSLCVNG SHIYNEEPQG PVRHRSSISG 370 380 390 400 410 420 SLPSSGSLQA VSSRFSEEGP RSTDDTWPRG SRSNSSSEAV LGQEELSAQA KVLAPGASHP 430 440 450 460 470 480 GEEEGARLPE GKPVQVATPI VASSEAVAEK EGARKEERKP RMGLFHHHHQ GLSRSELGRR 490 500 510 520 530 540 SSLGEKGGPI LGASPHHSSS GEEKAKSSWF GLREAKDPTQ KPSPHPVKPL SAAPVEGSPD 550 560 570 580 590 600 RKQSRSSLSI ALSSGLEKLK TVTSGSIQPV TQAPQAGQMV DTKRLKDSAV LDQSAKYYHL 610 620 630 640 650 THDELISLLL QRERELSQRD EHVQELESYI DRLLVRIMET SPTLLQIPPG PPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Rab11/Rip11 protein complex regulates apical membrane trafficking via recycling endosomes." Prekeris R., Klumperman J., Scheller R.H. Mol. Cell 6:1437-1448(2000) [PubMed: 11163216] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RAB11A, SUBCELLULAR LOCATION.
[2]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed: 10048485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"Defining a novel 75-kDa phosphoprotein associated with SS-A/Ro and identification of distinct human autoantibodies." Wang D., Buyon J.P., Zhu W., Chan E.K.L. J. Clin. Invest. 104:1265-1275(1999) [PubMed: 10545525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-653, INTERACTION WITH TROVE2, IDENTIFICATION AS ANTIGEN IN AUTOIMMUNE DISEASES, TISSUE SPECIFICITY, PHOSPHORYLATION. Tissue: Keratinocyte.
[5]	"Gaf-1, a gamma-SNAP-binding protein associated with the mitochondria." Chen D., Xu W., He P., Medrano E.E., Whiteheart S.W. J. Biol. Chem. 276:13127-13135(2001) [PubMed: 11278501] [Abstract] Cited for: INTERACTION WITH NAPG, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[6]	"Rab11-FIP4 interacts with Rab11 in a GTP-dependent manner and its overexpression condenses the Rab11 positive compartment in HeLa cells." Wallace D.M., Lindsay A.J., Hendrick A.G., McCaffrey M.W. Biochem. Biophys. Res. Commun. 299:770-779(2002) [PubMed: 12470645] [Abstract] Cited for: SUBUNIT.
[7]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-174, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188 AND SER-307, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307 AND SER-538, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307; SER-359 AND SER-553, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF334812 mRNA. Translation: AAK20892.1. AB020664 mRNA. Translation: BAA74880.1. Different initiation. BC035013 mRNA. Translation: AAH35013.1. AF153085 mRNA. Translation: AAF34356.1.
IPI	IPI00022033.
PIR	T17312.
RefSeq	NP_056285.1. NM_015470.2.
UniGene	Hs.24557.
3D structure databases
ProteinModelPortal	Q9BXF6.
SMR	Q9BXF6. Positions 15-168, 596-652.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BXF6. 7 interactions.
STRING	Q9BXF6.
PTM databases
PhosphoSite	Q9BXF6.
Polymorphism databases
DMDM	34223002.
Proteomic databases
PRIDE	Q9BXF6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000258098; ENSP00000258098; ENSG00000135631.
GeneID	26056.
KEGG	hsa:26056.
UCSC	uc002sit.2. human.
Organism-specific databases
CTD	26056.
GeneCards	GC02M073212.
H-InvDB	HIX0002158.
HGNC	HGNC:24845. RAB11FIP5.
HPA	HPA036407.
MIM	605536. gene.
neXtProt	NX_Q9BXF6.
PharmGKB	PA134971129.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09359.
GeneTree	ENSGT00530000063203.
HOGENOM	HBG714796.
HOVERGEN	HBG079127.
OMA	TQKPSPH.
OrthoDB	EOG49CQ7C.
PhylomeDB	Q9BXF6.
Gene expression databases
ArrayExpress	Q9BXF6.
Bgee	Q9BXF6.
CleanEx	HS_RAB11FIP5.
Genevestigator	Q9BXF6.
GermOnline	ENSG00000135631. Homo sapiens.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR019018. Rab-bd_FIP-RBD. [Graphical view]
KO	K12484.
Pfam	PF00168. C2. 1 hit. PF09457. RBD-FIP. 1 hit. [Graphical view]
SMART	SM00239. C2. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit.
PROSITE	PS50004. C2. 1 hit. PS51511. FIP_RBD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	47930.
SOURCE	Search...

Entry information

Entry name

RFIP5_HUMAN

Accession

Primary (citable) accession number: Q9BXF6
Secondary accession number(s): O94939, Q9P0M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.