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Q9BXF6

- RFIP5_HUMAN

UniProt

Q9BXF6 - RFIP5_HUMAN

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Protein
Rab11 family-interacting protein 5
Gene
RAB11FIP5, GAF1, KIAA0857, RIP11
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Involved in insulin granule exocytosis. May regulate V-ATPase intracellular transport in response to extracellular acidosis.2 Publications

GO - Molecular functioni

  1. gamma-tubulin binding Source: UniProtKB
  2. protein binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cellular response to acidity Source: UniProtKB
  2. insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  3. protein transport Source: UniProtKB-KW
  4. regulated secretory pathway Source: UniProtKB
  5. regulation of protein localization to cell surface Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Rab11 family-interacting protein 5
Short name:
Rab11-FIP5
Alternative name(s):
Gamma-SNAP-associated factor 1
Short name:
Gaf-1
Phosphoprotein pp75
Rab11-interacting protein Rip11
Gene namesi
Synonyms:GAF1, KIAA0857, RIP11
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:24845. RAB11FIP5.

Subcellular locationi

Cytoplasm. Recycling endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein By similarity. Golgi apparatus membrane; Peripheral membrane protein By similarity. Cytoplasmic vesiclesecretory vesicle membrane; Peripheral membrane protein By similarity. Mitochondrion membrane; Peripheral membrane protein 2 Publications

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. Golgi membrane Source: UniProtKB-SubCell
  3. early endosome Source: UniProtKB
  4. early endosome membrane Source: UniProtKB-SubCell
  5. intracellular membrane-bounded organelle Source: HPA
  6. microtubule organizing center Source: HPA
  7. mitochondrial outer membrane Source: UniProtKB
  8. recycling endosome Source: UniProtKB
  9. recycling endosome membrane Source: UniProtKB-SubCell
  10. secretory granule Source: UniProtKB
  11. transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane, Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134971129.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 653653Rab11 family-interacting protein 5
PRO_0000097307Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei176 – 1761Phosphoserine1 Publication
Modified residuei307 – 3071Phosphoserine2 Publications
Modified residuei357 – 3571Phosphoserine By similarity
Modified residuei494 – 4941Phosphoserine1 Publication
Modified residuei538 – 5381Phosphoserine2 Publications
Modified residuei547 – 5471Phosphoserine By similarity
Modified residuei553 – 5531Phosphoserine By similarity

Post-translational modificationi

Phosphorylated on serine and threonine residues. Phosphorylation at Ser-357 is PKA-dependent.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9BXF6.
PaxDbiQ9BXF6.
PRIDEiQ9BXF6.

PTM databases

PhosphoSiteiQ9BXF6.

Expressioni

Tissue specificityi

Detected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas.2 Publications

Gene expression databases

ArrayExpressiQ9BXF6.
BgeeiQ9BXF6.
CleanExiHS_RAB11FIP5.
GenevestigatoriQ9BXF6.

Organism-specific databases

HPAiHPA036406.
HPA036407.

Interactioni

Subunit structurei

Forms a heterooligomeric complex with RAB11FIP4. Binds NAPG and TROVE2. Binds RAB11A that has been activated by GTP binding.1 Publication

Protein-protein interaction databases

BioGridi117518. 15 interactions.
IntActiQ9BXF6. 7 interactions.
STRINGi9606.ENSP00000258098.

Structurei

3D structure databases

ProteinModelPortaliQ9BXF6.
SMRiQ9BXF6. Positions 21-169, 600-651.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 128123C2
Add
BLAST
Domaini586 – 64863FIP-RBD
Add
BLAST

Domaini

Binds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg2+ rather than Ca2+.

Sequence similaritiesi

Contains 1 C2 domain.
Contains 1 FIP-RBD domain.

Phylogenomic databases

eggNOGiNOG84049.
HOGENOMiHOG000234700.
HOVERGENiHBG079127.
KOiK12484.
OMAiVEKTHGC.
OrthoDBiEOG7WQ7SB.
PhylomeDBiQ9BXF6.
TreeFamiTF326172.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR019018. Rab-bd_FIP-RBD.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF09457. RBD-FIP. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51511. FIP_RBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BXF6-1 [UniParc]FASTAAdd to Basket

« Hide

MALVRGAEPA AGPSRWLPTH VQVTVLRARG LRGKSSGAGS TSDAYTVIQV    50
GREKYSTSVV EKTHGCPEWR EECSFELPPG ALDGLLRAQE ADAGPAPWAA 100
SSAAACELVL TTMHRSLIGV DKFLGQATVA LDEVFGAGRA QHTQWYKLHS 150
KPGKKEKERG EIEVTIQFTR NNLSASMFDL SMKDKPRSPF SKIRDKMKGK 200
KKYDLESASA ILPSSAIEDP DLGSLGKMGK AKGFFLRNKL RKSSLTQSNT 250
SLGSDSTLSS ASGSLAYQGP GAELLTRSPS RSSWLSTEGG RDSAQSPKLF 300
THKRTYSDEA NQMRVAPPRA LLDLQGHLDA ASRSSLCVNG SHIYNEEPQG 350
PVRHRSSISG SLPSSGSLQA VSSRFSEEGP RSTDDTWPRG SRSNSSSEAV 400
LGQEELSAQA KVLAPGASHP GEEEGARLPE GKPVQVATPI VASSEAVAEK 450
EGARKEERKP RMGLFHHHHQ GLSRSELGRR SSLGEKGGPI LGASPHHSSS 500
GEEKAKSSWF GLREAKDPTQ KPSPHPVKPL SAAPVEGSPD RKQSRSSLSI 550
ALSSGLEKLK TVTSGSIQPV TQAPQAGQMV DTKRLKDSAV LDQSAKYYHL 600
THDELISLLL QRERELSQRD EHVQELESYI DRLLVRIMET SPTLLQIPPG 650
PPK 653
Length:653
Mass (Da):70,415
Last modified:June 1, 2001 - v1
Checksum:i716725537AA9D86E
GO

Sequence cautioni

The sequence BAA74880.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1761Missing in AAH35013. 1 Publication
Sequence conflicti503 – 5031E → G in AAH35013. 1 Publication
Sequence conflicti599 – 5991H → N in AAH35013. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF334812 mRNA. Translation: AAK20892.1.
AB020664 mRNA. Translation: BAA74880.1. Different initiation.
BC035013 mRNA. Translation: AAH35013.1.
AF153085 mRNA. Translation: AAF34356.1.
CCDSiCCDS1923.1.
PIRiT17312.
RefSeqiNP_056285.1. NM_015470.2.
UniGeneiHs.24557.

Genome annotation databases

EnsembliENST00000258098; ENSP00000258098; ENSG00000135631.
GeneIDi26056.
KEGGihsa:26056.
UCSCiuc002sit.4. human.

Polymorphism databases

DMDMi34223002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF334812 mRNA. Translation: AAK20892.1 .
AB020664 mRNA. Translation: BAA74880.1 . Different initiation.
BC035013 mRNA. Translation: AAH35013.1 .
AF153085 mRNA. Translation: AAF34356.1 .
CCDSi CCDS1923.1.
PIRi T17312.
RefSeqi NP_056285.1. NM_015470.2.
UniGenei Hs.24557.

3D structure databases

ProteinModelPortali Q9BXF6.
SMRi Q9BXF6. Positions 21-169, 600-651.
ModBasei Search...

Protein-protein interaction databases

BioGridi 117518. 15 interactions.
IntActi Q9BXF6. 7 interactions.
STRINGi 9606.ENSP00000258098.

PTM databases

PhosphoSitei Q9BXF6.

Polymorphism databases

DMDMi 34223002.

Proteomic databases

MaxQBi Q9BXF6.
PaxDbi Q9BXF6.
PRIDEi Q9BXF6.

Protocols and materials databases

DNASUi 26056.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000258098 ; ENSP00000258098 ; ENSG00000135631 .
GeneIDi 26056.
KEGGi hsa:26056.
UCSCi uc002sit.4. human.

Organism-specific databases

CTDi 26056.
GeneCardsi GC02M073212.
HGNCi HGNC:24845. RAB11FIP5.
HPAi HPA036406.
HPA036407.
MIMi 605536. gene.
neXtProti NX_Q9BXF6.
PharmGKBi PA134971129.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG84049.
HOGENOMi HOG000234700.
HOVERGENi HBG079127.
KOi K12484.
OMAi VEKTHGC.
OrthoDBi EOG7WQ7SB.
PhylomeDBi Q9BXF6.
TreeFami TF326172.

Miscellaneous databases

GeneWikii RAB11FIP5.
GenomeRNAii 26056.
NextBioi 47930.
PROi Q9BXF6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9BXF6.
Bgeei Q9BXF6.
CleanExi HS_RAB11FIP5.
Genevestigatori Q9BXF6.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR000008. C2_dom.
IPR019018. Rab-bd_FIP-RBD.
[Graphical view ]
Pfami PF00168. C2. 1 hit.
PF09457. RBD-FIP. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS51511. FIP_RBD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Rab11/Rip11 protein complex regulates apical membrane trafficking via recycling endosomes."
    Prekeris R., Klumperman J., Scheller R.H.
    Mol. Cell 6:1437-1448(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RAB11A, SUBCELLULAR LOCATION.
  2. "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Defining a novel 75-kDa phosphoprotein associated with SS-A/Ro and identification of distinct human autoantibodies."
    Wang D., Buyon J.P., Zhu W., Chan E.K.L.
    J. Clin. Invest. 104:1265-1275(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-653, INTERACTION WITH TROVE2, IDENTIFICATION AS ANTIGEN IN AUTOIMMUNE DISEASES, TISSUE SPECIFICITY, PHOSPHORYLATION.
    Tissue: Keratinocyte.
  5. "Gaf-1, a gamma-SNAP-binding protein associated with the mitochondria."
    Chen D., Xu W., He P., Medrano E.E., Whiteheart S.W.
    J. Biol. Chem. 276:13127-13135(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NAPG, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  6. "Rab11-FIP4 interacts with Rab11 in a GTP-dependent manner and its overexpression condenses the Rab11 positive compartment in HeLa cells."
    Wallace D.M., Lindsay A.J., Hendrick A.G., McCaffrey M.W.
    Biochem. Biophys. Res. Commun. 299:770-779(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307 AND SER-538, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176; SER-307; SER-494 AND SER-538, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. "Rab11b and its effector Rip11 regulate the acidosis-induced traffic of V-ATPase in salivary ducts."
    Oehlke O., Martin H.W., Osterberg N., Roussa E.
    J. Cell. Physiol. 226:638-651(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiRFIP5_HUMAN
AccessioniPrimary (citable) accession number: Q9BXF6
Secondary accession number(s): O94939, Q9P0M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Antibodies against RIP11 are found in sera from patients with autoimmune diseases such as systemic lupus erythematosus (SLE) or Sjoegren syndrome (SS). It is also found in the sera from mothers of children with neonatal lupus erythematosus (NLE).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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