Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9BXC0 (HCAR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxycarboxylic acid receptor 1
Alternative name(s):
G-protein coupled receptor 104
G-protein coupled receptor 81
Gene names
Name:HCAR1
Synonyms:GPR104, GPR81, HCA1
ORF Names:FKSG80
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway. Ref.2

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed abundantly in brown and white fat. It also detectable at lower levels in liver, kidney, skeletal muscle, brain and pituitary. Not detected in frontal, temporal and occipital lobes of the cortex, basal forebrain, caudate nucleus, nucleus accumbens and hippocampus. Ref.1 Ref.2

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to estradiol

Inferred from direct assay PubMed 15831516. Source: BHF-UCL

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Hydroxycarboxylic acid receptor 1
PRO_0000069586

Regions

Topological domain1 – 2121Extracellular Potential
Transmembrane22 – 4221Helical; Name=1; Potential
Topological domain43 – 497Cytoplasmic Potential
Transmembrane50 – 7021Helical; Name=2; Potential
Topological domain71 – 8919Extracellular Potential
Transmembrane90 – 11021Helical; Name=3; Potential
Topological domain111 – 13020Cytoplasmic Potential
Transmembrane131 – 15121Helical; Name=4; Potential
Topological domain152 – 18231Extracellular Potential
Transmembrane183 – 20321Helical; Name=5; Potential
Topological domain204 – 22017Cytoplasmic Potential
Transmembrane221 – 24121Helical; Name=6; Potential
Topological domain242 – 26120Extracellular Potential
Transmembrane262 – 28120Helical; Name=7; Potential
Topological domain282 – 34665Cytoplasmic Potential

Amino acid modifications

Glycosylation31N-linked (GlcNAc...) Potential
Disulfide bond88 ↔ 165 By similarity

Natural variations

Natural variant431H → Q. Ref.8
Corresponds to variant rs35292336 [ dbSNP | Ensembl ].
VAR_025151
Natural variant2531D → E. Ref.8
VAR_025152
Natural variant2531D → H.
Corresponds to variant rs36124671 [ dbSNP | Ensembl ].
VAR_061218

Experimental info

Mutagenesis991R → A: Diminishes the response to L-lactate. Ref.2
Mutagenesis2331Y → A: Diminishes the response to L-lactate. Ref.2
Mutagenesis2401R → A: Diminishes the response to L-lactate. Ref.2
Mutagenesis2671T → A: Diminishes the response to L-lactate. Ref.2
Sequence conflict381C → Y in AAH95506. Ref.11
Sequence conflict1131R → K in AAH66883. Ref.11
Sequence conflict1871M → I in AAH95506. Ref.11
Sequence conflict2131A → T in AAH95506. Ref.11
Sequence conflict2221I → T in AAH95506. Ref.11

Sequences

Sequence LengthMass (Da)Tools
Q9BXC0 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: E0DB114EEB3A47A5

FASTA34639,295
        10         20         30         40         50         60 
MYNGSCCRIE GDTISQVMPP LLIVAFVLGA LGNGVALCGF CFHMKTWKPS TVYLFNLAVA 

        70         80         90        100        110        120 
DFLLMICLPF RTDYYLRRRH WAFGDIPCRV GLFTLAMNRA GSIVFLTVVA ADRYFKVVHP 

       130        140        150        160        170        180 
HHAVNTISTR VAAGIVCTLW ALVILGTVYL LLENHLCVQE TAVSCESFIM ESANGWHDIM 

       190        200        210        220        230        240 
FQLEFFMPLG IILFCSFKIV WSLRRRQQLA RQARMKKATR FIMVVAIVFI TCYLPSVSAR 

       250        260        270        280        290        300 
LYFLWTVPSS ACDPSVHGAL HITLSFTYMN SMLDPLVYYF SSPSFPKFYN KLKICSLKPK 

       310        320        330        340 
QPGHSKTQRP EEMPISNLGR RSCISVANSF QSQSDGQWDP HIVEWH 

« Hide

References

« Hide 'large scale' references
[1]"Discovery and mapping of ten novel G protein-coupled receptor genes."
Lee D.K., Nguyen T., Lynch K.R., Cheng R., Vanti W.B., Arkhitko O., Lewis T., Evans J.F., George S.R., O'Dowd B.F.
Gene 275:83-91(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[2]"Lactate inhibits lipolysis in fat cells through activation of an orphan G-protein-coupled receptor, GPR81."
Liu C., Wu J., Zhu J., Kuei C., Yu J., Shelton J., Sutton S.W., Li X., Yun S.J., Mirzadegan T., Mazur C., Kamme F., Lovenberg T.W.
J. Biol. Chem. 284:2811-2822(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-99; TYR-233; ARG-240 AND THR-267.
[3]"Molecular cloning of FKSG80, a novel gene encoding a putative chemokine receptor."
Wang Y.-G., Gong L.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S., Tsutsumi S., Aburatani H., Asai K., Akiyama Y.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
King M.M., Aronstam R.S., Sharma S.V.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
[7]"Identification of G protein-coupled receptor genes from the human genome sequence."
Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S.
FEBS Lett. 520:97-101(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]SeattleSNPs variation discovery resource
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLN-43 AND GLU-253.
[9]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[12]"International union of basic and clinical pharmacology. LXXXII: nomenclature and classification of hydroxy-carboxylic acid receptors (GPR81, GPR109A, and GPR109B)."
Offermanns S., Colletti S.L., Lovenberg T.W., Semple G., Wise A., Ijzerman A.P.
Pharmacol. Rev. 63:269-290(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Web resources

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF411110 Genomic DNA. Translation: AAL26481.1.
EU809458 mRNA. Translation: ACJ03844.1.
AF345568 mRNA. Translation: AAK29071.1.
AB065866 Genomic DNA. Translation: BAC06084.1.
AY507143 mRNA. Translation: AAR92485.1.
AK313955 mRNA. Translation: BAG36671.1.
AB083631 Genomic DNA. Translation: BAB89344.1.
DQ157221 Genomic DNA. Translation: AAZ67915.1.
AC026333 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW98337.1.
BC066881 mRNA. Translation: AAH66881.1.
BC066882 mRNA. Translation: AAH66882.1.
BC066883 mRNA. Translation: AAH66883.1.
BC067484 mRNA. Translation: AAH67484.1.
BC095506 mRNA. Translation: AAH95506.1.
RefSeqNP_115943.1. NM_032554.3.
UniGeneHs.610873.
Hs.711001.

3D structure databases

ProteinModelPortalQ9BXC0.
SMRQ9BXC0. Positions 18-285.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9BXC0. 1 interaction.
STRING9606.ENSP00000349478.

Chemistry

ChEMBLCHEMBL1075101.
GuidetoPHARMACOLOGY311.

Protein family/group databases

TCDB9.A.14.13.4. the g-protein-coupled receptor (gpcr) family.
GPCRDBSearch...

PTM databases

PhosphoSiteQ9BXC0.

Polymorphism databases

DMDM47117701.

Proteomic databases

PaxDbQ9BXC0.
PRIDEQ9BXC0.

Protocols and materials databases

DNASU27198.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356987; ENSP00000349478; ENSG00000196917.
ENST00000432564; ENSP00000389255; ENSG00000196917.
ENST00000436083; ENSP00000409980; ENSG00000196917.
GeneID27198.
KEGGhsa:27198.
UCSCuc001ucz.3. human.

Organism-specific databases

CTD27198.
GeneCardsGC12M123104.
H-InvDBHIX0026348.
HGNCHGNC:4532. HCAR1.
MIM606923. gene.
neXtProtNX_Q9BXC0.
PharmGKBPA28925.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG148165.
HOVERGENHBG051680.
InParanoidQ9BXC0.
KOK08401.
OMALAMNRAG.
OrthoDBEOG7R56SR.
PhylomeDBQ9BXC0.
TreeFamTF330775.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeQ9BXC0.
CleanExHS_GPR81.
GenevestigatorQ9BXC0.

Family and domain databases

InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR028016. HCAR1_rcpt.
[Graphical view]
PANTHERPTHR24231:SF0. PTHR24231:SF0. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGPR81.
GenomeRNAi27198.
NextBio50062.
PROQ9BXC0.
SOURCESearch...

Entry information

Entry nameHCAR1_HUMAN
AccessionPrimary (citable) accession number: Q9BXC0
Secondary accession number(s): B2R9X4 expand/collapse secondary AC list , Q3Y5J3, Q4VBN1, Q6NXU5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries