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Protein

Testis-specific serine/threonine-protein kinase 1

Gene

TSSK1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-288' of TSKS. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Kinase activity is specifically inhibited by 2 classes of compounds: biphenyl compounds (1,1'-(biphenyl-4,4'-diyl)bis(2,2-dihydroxyethanone)) and 1,2,7-trialky-1H-imidazo[4,5-g]quinoxalin-6-one. Activated by phosphorylation on Thr-174 and potentially by autophosphorylation.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS09040-MONOMER.
SignaLinkiQ9BXA7.

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 1 (EC:2.7.11.1)
Short name:
TSK-1
Short name:
TSK1
Short name:
TSSK-1
Short name:
Testis-specific kinase 1
Alternative name(s):
Serine/threonine-protein kinase 22A
Gene namesi
Name:TSSK1B
Synonyms:SPOGA1, SPOGA4, STK22A, STK22D, TSSK1
ORF Names:FKSG81
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:14968. TSSK1B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi174T → A: Loss of kinase activity. 1 Publication1
Mutagenesisi174T → E: Constitutively active. 1 Publication1

Organism-specific databases

DisGeNETi83942.
OpenTargetsiENSG00000212122.
PharmGKBiPA37944.

Chemistry databases

ChEMBLiCHEMBL6003.
GuidetoPHARMACOLOGYi2257.

Polymorphism and mutation databases

BioMutaiTSSK1B.
DMDMi30316282.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867661 – 367Testis-specific serine/threonine-protein kinase 1Add BLAST367

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei174Phosphothreonine1 Publication1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9BXA7.
PeptideAtlasiQ9BXA7.
PRIDEiQ9BXA7.

PTM databases

iPTMnetiQ9BXA7.
PhosphoSitePlusiQ9BXA7.

Expressioni

Tissue specificityi

Testis-specific. Present in sperm (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000212122.
CleanExiHS_TSSK1B.
ExpressionAtlasiQ9BXA7. baseline and differential.
GenevisibleiQ9BXA7. HS.

Organism-specific databases

HPAiHPA027827.

Interactioni

Subunit structurei

Interacts with TSSK2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EFSO432813EBI-6423734,EBI-718488

Protein-protein interaction databases

BioGridi123825. 14 interactors.
IntActiQ9BXA7. 4 interactors.
STRINGi9606.ENSP00000375081.

Chemistry databases

BindingDBiQ9BXA7.

Structurei

3D structure databases

ProteinModelPortaliQ9BXA7.
SMRiQ9BXA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 272Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ9BXA7.
KOiK08811.
OMAiVRCAVNT.
OrthoDBiEOG091G0EZI.
PhylomeDBiQ9BXA7.
TreeFamiTF352374.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BXA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDAAVLKRR GYLLGINLGE GSYAKVKSAY SERLKFNVAI KIIDRKKAPA
60 70 80 90 100
DFLEKFLPRE IEILAMLNHC SIIKTYEIFE TSHGKVYIVM ELAVQGDLLE
110 120 130 140 150
LIKTRGALHE DEARKKFHQL SLAIKYCHDL DVVHRDLKCD NLLLDKDFNI
160 170 180 190 200
KLSDFSFSKR CLRDDSGRMA LSKTFCGSPA YAAPEVLQGI PYQPKVYDIW
210 220 230 240 250
SLGVILYIMV CGSMPYDDSN IKKMLRIQKE HRVNFPRSKH LTGECKDLIY
260 270 280 290 300
HMLQPDVNRR LHIDEILSHC WMQPKARGSP SVAINKEGES SRGTEPLWTP
310 320 330 340 350
EPGSDKKSAT KLEPEGEAQP QAQPETKPEG TAMQMSRQSE ILGFPSKPST
360
METEEGPPQQ PPETRAQ
Length:367
Mass (Da):41,618
Last modified:June 1, 2001 - v1
Checksum:i1B740988894A1589
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti345P → L in BAG36136 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04123550A → T.1 PublicationCorresponds to variant rs747955728dbSNPEnsembl.1
Natural variantiVAR_04123683H → Y.1 PublicationCorresponds to variant rs55930004dbSNPEnsembl.1
Natural variantiVAR_041237233V → L.1 PublicationCorresponds to variant rs55940513dbSNPEnsembl.1
Natural variantiVAR_041238237R → C.1 PublicationCorresponds to variant rs55738530dbSNPEnsembl.1
Natural variantiVAR_041239288G → W.1 PublicationCorresponds to variant rs34696815dbSNPEnsembl.1
Natural variantiVAR_041240293G → E.1 PublicationCorresponds to variant rs11953478dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028964 mRNA. Translation: AAK27734.1.
AF348076 mRNA. Translation: AAK29413.1.
AK313332 mRNA. Translation: BAG36136.1.
AC010431 Genomic DNA. No translation available.
BC022515 mRNA. Translation: AAH22515.1.
CCDSiCCDS4112.1.
RefSeqiNP_114417.1. NM_032028.3.
UniGeneiHs.519507.

Genome annotation databases

EnsembliENST00000390666; ENSP00000375081; ENSG00000212122.
GeneIDi83942.
KEGGihsa:83942.
UCSCiuc003kqm.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028964 mRNA. Translation: AAK27734.1.
AF348076 mRNA. Translation: AAK29413.1.
AK313332 mRNA. Translation: BAG36136.1.
AC010431 Genomic DNA. No translation available.
BC022515 mRNA. Translation: AAH22515.1.
CCDSiCCDS4112.1.
RefSeqiNP_114417.1. NM_032028.3.
UniGeneiHs.519507.

3D structure databases

ProteinModelPortaliQ9BXA7.
SMRiQ9BXA7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123825. 14 interactors.
IntActiQ9BXA7. 4 interactors.
STRINGi9606.ENSP00000375081.

Chemistry databases

BindingDBiQ9BXA7.
ChEMBLiCHEMBL6003.
GuidetoPHARMACOLOGYi2257.

PTM databases

iPTMnetiQ9BXA7.
PhosphoSitePlusiQ9BXA7.

Polymorphism and mutation databases

BioMutaiTSSK1B.
DMDMi30316282.

Proteomic databases

PaxDbiQ9BXA7.
PeptideAtlasiQ9BXA7.
PRIDEiQ9BXA7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000390666; ENSP00000375081; ENSG00000212122.
GeneIDi83942.
KEGGihsa:83942.
UCSCiuc003kqm.2. human.

Organism-specific databases

CTDi83942.
DisGeNETi83942.
GeneCardsiTSSK1B.
HGNCiHGNC:14968. TSSK1B.
HPAiHPA027827.
MIMi610709. gene.
neXtProtiNX_Q9BXA7.
OpenTargetsiENSG00000212122.
PharmGKBiPA37944.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ9BXA7.
KOiK08811.
OMAiVRCAVNT.
OrthoDBiEOG091G0EZI.
PhylomeDBiQ9BXA7.
TreeFamiTF352374.

Enzyme and pathway databases

BioCyciZFISH:HS09040-MONOMER.
SignaLinkiQ9BXA7.

Miscellaneous databases

GenomeRNAii83942.
PROiQ9BXA7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000212122.
CleanExiHS_TSSK1B.
ExpressionAtlasiQ9BXA7. baseline and differential.
GenevisibleiQ9BXA7. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTSSK1_HUMAN
AccessioniPrimary (citable) accession number: Q9BXA7
Secondary accession number(s): B2R8D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

TSSK1B might be used as a target for male contraception or and intra-vaginal spermicides.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.