Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9BXA7

- TSSK1_HUMAN

UniProt

Q9BXA7 - TSSK1_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Testis-specific serine/threonine-protein kinase 1

Gene

TSSK1B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-288' of TSKS. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Kinase activity is specifically inhibited by 2 classes of compounds: biphenyl compounds (1,1'-(biphenyl-4,4'-diyl)bis(2,2-dihydroxyethanone)) and 1,2,7-trialky-1H-imidazo[4,5-g]quinoxalin-6-one. Activated by phosphorylation on Thr-174 and potentially by autophosphorylation.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. protein phosphorylation Source: UniProtKB
  3. spermatid development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ9BXA7.

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 1 (EC:2.7.11.1)
Short name:
TSK-1
Short name:
TSK1
Short name:
TSSK-1
Short name:
Testis-specific kinase 1
Alternative name(s):
Serine/threonine-protein kinase 22A
Gene namesi
Name:TSSK1B
Synonyms:SPOGA1, SPOGA4, STK22A, STK22D, TSSK1
ORF Names:FKSG81
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:14968. TSSK1B.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmic vesiclesecretory vesicleacrosome By similarity. Cell projectionciliumflagellum By similarity
Note: In spermatozoa, present in the sperm head and in the flagellum.By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. motile cilium Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi174 – 1741T → A: Loss of kinase activity. 1 Publication
Mutagenesisi174 – 1741T → E: Constitutively active. 1 Publication

Organism-specific databases

PharmGKBiPA37944.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 367367Testis-specific serine/threonine-protein kinase 1PRO_0000086766Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei174 – 1741Phosphothreonine1 Publication

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9BXA7.
PRIDEiQ9BXA7.

PTM databases

PhosphoSiteiQ9BXA7.

Expressioni

Tissue specificityi

Testis-specific. Present in sperm (at protein level).2 Publications

Gene expression databases

BgeeiQ9BXA7.
CleanExiHS_TSSK1B.
ExpressionAtlasiQ9BXA7. baseline.
GenevestigatoriQ9BXA7.

Organism-specific databases

HPAiHPA027827.

Interactioni

Subunit structurei

Interacts with TSSK2.1 Publication

Protein-protein interaction databases

BioGridi123825. 11 interactions.
IntActiQ9BXA7. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9BXA7.
SMRiQ9BXA7. Positions 12-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 272261Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120521.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ9BXA7.
KOiK08811.
OMAiWVQPKAR.
OrthoDBiEOG7P8P8F.
PhylomeDBiQ9BXA7.
TreeFamiTF352374.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BXA7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDAAVLKRR GYLLGINLGE GSYAKVKSAY SERLKFNVAI KIIDRKKAPA
60 70 80 90 100
DFLEKFLPRE IEILAMLNHC SIIKTYEIFE TSHGKVYIVM ELAVQGDLLE
110 120 130 140 150
LIKTRGALHE DEARKKFHQL SLAIKYCHDL DVVHRDLKCD NLLLDKDFNI
160 170 180 190 200
KLSDFSFSKR CLRDDSGRMA LSKTFCGSPA YAAPEVLQGI PYQPKVYDIW
210 220 230 240 250
SLGVILYIMV CGSMPYDDSN IKKMLRIQKE HRVNFPRSKH LTGECKDLIY
260 270 280 290 300
HMLQPDVNRR LHIDEILSHC WMQPKARGSP SVAINKEGES SRGTEPLWTP
310 320 330 340 350
EPGSDKKSAT KLEPEGEAQP QAQPETKPEG TAMQMSRQSE ILGFPSKPST
360
METEEGPPQQ PPETRAQ
Length:367
Mass (Da):41,618
Last modified:June 1, 2001 - v1
Checksum:i1B740988894A1589
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti345 – 3451P → L in BAG36136. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti50 – 501A → T.1 Publication
VAR_041235
Natural varianti83 – 831H → Y.1 Publication
Corresponds to variant rs55930004 [ dbSNP | Ensembl ].
VAR_041236
Natural varianti233 – 2331V → L.1 Publication
Corresponds to variant rs55940513 [ dbSNP | Ensembl ].
VAR_041237
Natural varianti237 – 2371R → C.1 Publication
Corresponds to variant rs55738530 [ dbSNP | Ensembl ].
VAR_041238
Natural varianti288 – 2881G → W.1 Publication
Corresponds to variant rs34696815 [ dbSNP | Ensembl ].
VAR_041239
Natural varianti293 – 2931G → E.1 Publication
Corresponds to variant rs11953478 [ dbSNP | Ensembl ].
VAR_041240

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028964 mRNA. Translation: AAK27734.1.
AF348076 mRNA. Translation: AAK29413.1.
AK313332 mRNA. Translation: BAG36136.1.
AC010431 Genomic DNA. No translation available.
BC022515 mRNA. Translation: AAH22515.1.
CCDSiCCDS4112.1.
RefSeqiNP_114417.1. NM_032028.3.
UniGeneiHs.519507.

Genome annotation databases

EnsembliENST00000390666; ENSP00000375081; ENSG00000212122.
GeneIDi83942.
KEGGihsa:83942.
UCSCiuc003kqm.2. human.

Polymorphism databases

DMDMi30316282.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY028964 mRNA. Translation: AAK27734.1 .
AF348076 mRNA. Translation: AAK29413.1 .
AK313332 mRNA. Translation: BAG36136.1 .
AC010431 Genomic DNA. No translation available.
BC022515 mRNA. Translation: AAH22515.1 .
CCDSi CCDS4112.1.
RefSeqi NP_114417.1. NM_032028.3.
UniGenei Hs.519507.

3D structure databases

ProteinModelPortali Q9BXA7.
SMRi Q9BXA7. Positions 12-331.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123825. 11 interactions.
IntActi Q9BXA7. 1 interaction.

Chemistry

BindingDBi Q9BXA7.
ChEMBLi CHEMBL6003.
GuidetoPHARMACOLOGYi 2257.

PTM databases

PhosphoSitei Q9BXA7.

Polymorphism databases

DMDMi 30316282.

Proteomic databases

PaxDbi Q9BXA7.
PRIDEi Q9BXA7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000390666 ; ENSP00000375081 ; ENSG00000212122 .
GeneIDi 83942.
KEGGi hsa:83942.
UCSCi uc003kqm.2. human.

Organism-specific databases

CTDi 83942.
GeneCardsi GC05M112768.
HGNCi HGNC:14968. TSSK1B.
HPAi HPA027827.
MIMi 610709. gene.
neXtProti NX_Q9BXA7.
PharmGKBi PA37944.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00770000120521.
HOGENOMi HOG000233016.
HOVERGENi HBG101110.
InParanoidi Q9BXA7.
KOi K08811.
OMAi WVQPKAR.
OrthoDBi EOG7P8P8F.
PhylomeDBi Q9BXA7.
TreeFami TF352374.

Enzyme and pathway databases

SignaLinki Q9BXA7.

Miscellaneous databases

GenomeRNAii 83942.
NextBioi 73081.
PROi Q9BXA7.
SOURCEi Search...

Gene expression databases

Bgeei Q9BXA7.
CleanExi HS_TSSK1B.
ExpressionAtlasi Q9BXA7. baseline.
Genevestigatori Q9BXA7.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. A TSSK member is present in the equatorial segment of human sperm."
    Hao Z., Jha K.N., Kim Y.H., Vemuganti S., Westbrook V.A., Chertihin O., Markgraf K., Flickinger C.J., Coppola M., Herr J.C., Visconti P.E.
    Mol. Hum. Reprod. 10:433-444(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH TSSK2.
  2. "Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate."
    Jaleel M., McBride A., Lizcano J.M., Deak M., Toth R., Morrice N.A., Alessi D.R.
    FEBS Lett. 579:1417-1423(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, AUTOPHOSPHORYLATION, PHOSPHORYLATION AT THR-174, MUTAGENESIS OF THR-174.
  3. "Molecular cloning and characterization of FKSG81, a novel gene located on human chromosome 5."
    Wang Y.-G., Gong L.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  5. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Hippocampus.
  7. "Validation of a testis specific serine/threonine kinase [TSSK] family and the substrate of TSSK1 & 2, TSKS, as contraceptive targets."
    Xu B., Hao Z., Jha K.N., Digilio L., Urekar C., Kim Y.H., Pulido S., Flickinger C.J., Herr J.C.
    Soc. Reprod. Fertil. Suppl. 63:87-101(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PUTATIVE CONTRACEPTIVE TARGET.
  8. "Identification of peptide substrate and small molecule inhibitors of testis-specific serine/threonine kinase1 (TSSK1) by the developed assays."
    Zhang L., Yan Y., Liu Z., Abliz Z., Liu G.
    J. Med. Chem. 52:4419-4428(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, FUNCTION.
  9. "Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis."
    Li Y., Sosnik J., Brassard L., Reese M., Spiridonov N.A., Bates T.C., Johnson G.R., Anguita J., Visconti P.E., Salicioni A.M.
    Mol. Hum. Reprod. 17:42-56(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  10. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-50; TYR-83; LEU-233; CYS-237; TRP-288 AND GLU-293.

Entry informationi

Entry nameiTSSK1_HUMAN
AccessioniPrimary (citable) accession number: Q9BXA7
Secondary accession number(s): B2R8D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

TSSK1B might be used as a target for male contraception or and intra-vaginal spermicides.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3