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Q9BXA7 (TSSK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Testis-specific serine/threonine-protein kinase 1

Short name=TSK-1
Short name=TSK1
Short name=TSSK-1
Short name=Testis-specific kinase 1
EC=2.7.11.1
Alternative name(s):
Serine/threonine-protein kinase 22A
Gene names
Name:TSSK1B
Synonyms:SPOGA1, SPOGA4, STK22A, STK22D, TSSK1
ORF Names:FKSG81
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-288' of TSKS. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body. Ref.2 Ref.8

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Kinase activity is specifically inhibited by 2 classes of compounds: biphenyl compounds (1,1'-(biphenyl-4,4'-diyl)bis(2,2-dihydroxyethanone)) and 1,2,7-trialky-1H-imidazo[4,5-g]quinoxalin-6-one. Activated by phosphorylation on Thr-174 and potentially by autophosphorylation. Ref.2 Ref.8

Subunit structure

Interacts with TSSK2. Ref.1

Subcellular location

Cytoplasm By similarity. Cytoplasmic vesiclesecretory vesicleacrosome By similarity. Cell projectionciliumflagellum By similarity. Note: In spermatozoa, present in the sperm head and in the flagellum By similarity.

Tissue specificity

Testis-specific. Present in sperm (at protein level). Ref.1 Ref.9

Post-translational modification

Autophosphorylated. Ref.2

Miscellaneous

TSSK1B might be used as a target for male contraception or and intra-vaginal spermicides (Ref.7).

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367Testis-specific serine/threonine-protein kinase 1
PRO_0000086766

Regions

Domain12 – 272261Protein kinase
Nucleotide binding18 – 269ATP By similarity

Sites

Active site1361Proton acceptor By similarity
Binding site411ATP By similarity

Amino acid modifications

Modified residue1741Phosphothreonine Ref.2

Natural variations

Natural variant501A → T. Ref.10
VAR_041235
Natural variant831H → Y. Ref.10
Corresponds to variant rs55930004 [ dbSNP | Ensembl ].
VAR_041236
Natural variant2331V → L. Ref.10
Corresponds to variant rs55940513 [ dbSNP | Ensembl ].
VAR_041237
Natural variant2371R → C. Ref.10
Corresponds to variant rs55738530 [ dbSNP | Ensembl ].
VAR_041238
Natural variant2881G → W. Ref.10
Corresponds to variant rs34696815 [ dbSNP | Ensembl ].
VAR_041239
Natural variant2931G → E. Ref.10
Corresponds to variant rs11953478 [ dbSNP | Ensembl ].
VAR_041240

Experimental info

Mutagenesis1741T → A: Loss of kinase activity. Ref.2
Mutagenesis1741T → E: Constitutively active. Ref.2
Sequence conflict3451P → L in BAG36136. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9BXA7 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 1B740988894A1589

FASTA36741,618
        10         20         30         40         50         60 
MDDAAVLKRR GYLLGINLGE GSYAKVKSAY SERLKFNVAI KIIDRKKAPA DFLEKFLPRE 

        70         80         90        100        110        120 
IEILAMLNHC SIIKTYEIFE TSHGKVYIVM ELAVQGDLLE LIKTRGALHE DEARKKFHQL 

       130        140        150        160        170        180 
SLAIKYCHDL DVVHRDLKCD NLLLDKDFNI KLSDFSFSKR CLRDDSGRMA LSKTFCGSPA 

       190        200        210        220        230        240 
YAAPEVLQGI PYQPKVYDIW SLGVILYIMV CGSMPYDDSN IKKMLRIQKE HRVNFPRSKH 

       250        260        270        280        290        300 
LTGECKDLIY HMLQPDVNRR LHIDEILSHC WMQPKARGSP SVAINKEGES SRGTEPLWTP 

       310        320        330        340        350        360 
EPGSDKKSAT KLEPEGEAQP QAQPETKPEG TAMQMSRQSE ILGFPSKPST METEEGPPQQ 


PPETRAQ 

« Hide

References

« Hide 'large scale' references
[1]"Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. A TSSK member is present in the equatorial segment of human sperm."
Hao Z., Jha K.N., Kim Y.H., Vemuganti S., Westbrook V.A., Chertihin O., Markgraf K., Flickinger C.J., Coppola M., Herr J.C., Visconti P.E.
Mol. Hum. Reprod. 10:433-444(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH TSSK2.
[2]"Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate."
Jaleel M., McBride A., Lizcano J.M., Deak M., Toth R., Morrice N.A., Alessi D.R.
FEBS Lett. 579:1417-1423(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, AUTOPHOSPHORYLATION, PHOSPHORYLATION AT THR-174, MUTAGENESIS OF THR-174.
[3]"Molecular cloning and characterization of FKSG81, a novel gene located on human chromosome 5."
Wang Y.-G., Gong L.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Hippocampus.
[7]"Validation of a testis specific serine/threonine kinase [TSSK] family and the substrate of TSSK1 & 2, TSKS, as contraceptive targets."
Xu B., Hao Z., Jha K.N., Digilio L., Urekar C., Kim Y.H., Pulido S., Flickinger C.J., Herr J.C.
Soc. Reprod. Fertil. Suppl. 63:87-101(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PUTATIVE CONTRACEPTIVE TARGET.
[8]"Identification of peptide substrate and small molecule inhibitors of testis-specific serine/threonine kinase1 (TSSK1) by the developed assays."
Zhang L., Yan Y., Liu Z., Abliz Z., Liu G.
J. Med. Chem. 52:4419-4428(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION, FUNCTION.
[9]"Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis."
Li Y., Sosnik J., Brassard L., Reese M., Spiridonov N.A., Bates T.C., Johnson G.R., Anguita J., Visconti P.E., Salicioni A.M.
Mol. Hum. Reprod. 17:42-56(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[10]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-50; TYR-83; LEU-233; CYS-237; TRP-288 AND GLU-293.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY028964 mRNA. Translation: AAK27734.1.
AF348076 mRNA. Translation: AAK29413.1.
AK313332 mRNA. Translation: BAG36136.1.
AC010431 Genomic DNA. No translation available.
BC022515 mRNA. Translation: AAH22515.1.
RefSeqNP_114417.1. NM_032028.3.
UniGeneHs.519507.

3D structure databases

ProteinModelPortalQ9BXA7.
SMRQ9BXA7. Positions 12-292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123825. 1 interaction.
IntActQ9BXA7. 1 interaction.

Chemistry

BindingDBQ9BXA7.
ChEMBLCHEMBL6003.
GuidetoPHARMACOLOGY2257.

PTM databases

PhosphoSiteQ9BXA7.

Polymorphism databases

DMDM30316282.

Proteomic databases

PaxDbQ9BXA7.
PRIDEQ9BXA7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000390666; ENSP00000375081; ENSG00000212122.
GeneID83942.
KEGGhsa:83942.
UCSCuc003kqm.2. human.

Organism-specific databases

CTD83942.
GeneCardsGC05M112801.
HGNCHGNC:14968. TSSK1B.
HPAHPA027827.
MIM610709. gene.
neXtProtNX_Q9BXA7.
PharmGKBPA37944.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233016.
HOVERGENHBG101110.
InParanoidQ9BXA7.
KOK08811.
OMAQPKARGL.
OrthoDBEOG7P8P8F.
PhylomeDBQ9BXA7.
TreeFamTF352374.

Enzyme and pathway databases

SignaLinkQ9BXA7.

Gene expression databases

ArrayExpressQ9BXA7.
BgeeQ9BXA7.
CleanExHS_TSSK1B.
GenevestigatorQ9BXA7.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi83942.
NextBio73081.
PROQ9BXA7.
SOURCESearch...

Entry information

Entry nameTSSK1_HUMAN
AccessionPrimary (citable) accession number: Q9BXA7
Secondary accession number(s): B2R8D9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM