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Q9BXA7

- TSSK1_HUMAN

UniProt

Q9BXA7 - TSSK1_HUMAN

Protein

Testis-specific serine/threonine-protein kinase 1

Gene

TSSK1B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates 'Ser-288' of TSKS. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Kinase activity is specifically inhibited by 2 classes of compounds: biphenyl compounds (1,1'-(biphenyl-4,4'-diyl)bis(2,2-dihydroxyethanone)) and 1,2,7-trialky-1H-imidazo[4,5-g]quinoxalin-6-one. Activated by phosphorylation on Thr-174 and potentially by autophosphorylation.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411ATPPROSITE-ProRule annotation
    Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. protein phosphorylation Source: UniProtKB
    3. spermatid development Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Differentiation, Spermatogenesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiQ9BXA7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Testis-specific serine/threonine-protein kinase 1 (EC:2.7.11.1)
    Short name:
    TSK-1
    Short name:
    TSK1
    Short name:
    TSSK-1
    Short name:
    Testis-specific kinase 1
    Alternative name(s):
    Serine/threonine-protein kinase 22A
    Gene namesi
    Name:TSSK1B
    Synonyms:SPOGA1, SPOGA4, STK22A, STK22D, TSSK1
    ORF Names:FKSG81
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:14968. TSSK1B.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmic vesiclesecretory vesicleacrosome By similarity. Cell projectionciliumflagellum By similarity
    Note: In spermatozoa, present in the sperm head and in the flagellum.By similarity

    GO - Cellular componenti

    1. acrosomal vesicle Source: UniProtKB-SubCell
    2. motile cilium Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoplasmic vesicle

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi174 – 1741T → A: Loss of kinase activity. 1 Publication
    Mutagenesisi174 – 1741T → E: Constitutively active. 1 Publication

    Organism-specific databases

    PharmGKBiPA37944.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Testis-specific serine/threonine-protein kinase 1PRO_0000086766Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei174 – 1741Phosphothreonine1 Publication

    Post-translational modificationi

    Autophosphorylated.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9BXA7.
    PRIDEiQ9BXA7.

    PTM databases

    PhosphoSiteiQ9BXA7.

    Expressioni

    Tissue specificityi

    Testis-specific. Present in sperm (at protein level).2 Publications

    Gene expression databases

    ArrayExpressiQ9BXA7.
    BgeeiQ9BXA7.
    CleanExiHS_TSSK1B.
    GenevestigatoriQ9BXA7.

    Organism-specific databases

    HPAiHPA027827.

    Interactioni

    Subunit structurei

    Interacts with TSSK2.1 Publication

    Protein-protein interaction databases

    BioGridi123825. 1 interaction.
    IntActiQ9BXA7. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BXA7.
    SMRiQ9BXA7. Positions 1-331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 272261Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233016.
    HOVERGENiHBG101110.
    InParanoidiQ9BXA7.
    KOiK08811.
    OMAiWVQPKAR.
    OrthoDBiEOG7P8P8F.
    PhylomeDBiQ9BXA7.
    TreeFamiTF352374.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9BXA7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDDAAVLKRR GYLLGINLGE GSYAKVKSAY SERLKFNVAI KIIDRKKAPA    50
    DFLEKFLPRE IEILAMLNHC SIIKTYEIFE TSHGKVYIVM ELAVQGDLLE 100
    LIKTRGALHE DEARKKFHQL SLAIKYCHDL DVVHRDLKCD NLLLDKDFNI 150
    KLSDFSFSKR CLRDDSGRMA LSKTFCGSPA YAAPEVLQGI PYQPKVYDIW 200
    SLGVILYIMV CGSMPYDDSN IKKMLRIQKE HRVNFPRSKH LTGECKDLIY 250
    HMLQPDVNRR LHIDEILSHC WMQPKARGSP SVAINKEGES SRGTEPLWTP 300
    EPGSDKKSAT KLEPEGEAQP QAQPETKPEG TAMQMSRQSE ILGFPSKPST 350
    METEEGPPQQ PPETRAQ 367
    Length:367
    Mass (Da):41,618
    Last modified:June 1, 2001 - v1
    Checksum:i1B740988894A1589
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti345 – 3451P → L in BAG36136. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti50 – 501A → T.1 Publication
    VAR_041235
    Natural varianti83 – 831H → Y.1 Publication
    Corresponds to variant rs55930004 [ dbSNP | Ensembl ].
    VAR_041236
    Natural varianti233 – 2331V → L.1 Publication
    Corresponds to variant rs55940513 [ dbSNP | Ensembl ].
    VAR_041237
    Natural varianti237 – 2371R → C.1 Publication
    Corresponds to variant rs55738530 [ dbSNP | Ensembl ].
    VAR_041238
    Natural varianti288 – 2881G → W.1 Publication
    Corresponds to variant rs34696815 [ dbSNP | Ensembl ].
    VAR_041239
    Natural varianti293 – 2931G → E.1 Publication
    Corresponds to variant rs11953478 [ dbSNP | Ensembl ].
    VAR_041240

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY028964 mRNA. Translation: AAK27734.1.
    AF348076 mRNA. Translation: AAK29413.1.
    AK313332 mRNA. Translation: BAG36136.1.
    AC010431 Genomic DNA. No translation available.
    BC022515 mRNA. Translation: AAH22515.1.
    CCDSiCCDS4112.1.
    RefSeqiNP_114417.1. NM_032028.3.
    UniGeneiHs.519507.

    Genome annotation databases

    EnsembliENST00000390666; ENSP00000375081; ENSG00000212122.
    GeneIDi83942.
    KEGGihsa:83942.
    UCSCiuc003kqm.2. human.

    Polymorphism databases

    DMDMi30316282.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY028964 mRNA. Translation: AAK27734.1 .
    AF348076 mRNA. Translation: AAK29413.1 .
    AK313332 mRNA. Translation: BAG36136.1 .
    AC010431 Genomic DNA. No translation available.
    BC022515 mRNA. Translation: AAH22515.1 .
    CCDSi CCDS4112.1.
    RefSeqi NP_114417.1. NM_032028.3.
    UniGenei Hs.519507.

    3D structure databases

    ProteinModelPortali Q9BXA7.
    SMRi Q9BXA7. Positions 1-331.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123825. 1 interaction.
    IntActi Q9BXA7. 1 interaction.

    Chemistry

    BindingDBi Q9BXA7.
    ChEMBLi CHEMBL6003.
    GuidetoPHARMACOLOGYi 2257.

    PTM databases

    PhosphoSitei Q9BXA7.

    Polymorphism databases

    DMDMi 30316282.

    Proteomic databases

    PaxDbi Q9BXA7.
    PRIDEi Q9BXA7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000390666 ; ENSP00000375081 ; ENSG00000212122 .
    GeneIDi 83942.
    KEGGi hsa:83942.
    UCSCi uc003kqm.2. human.

    Organism-specific databases

    CTDi 83942.
    GeneCardsi GC05M112801.
    HGNCi HGNC:14968. TSSK1B.
    HPAi HPA027827.
    MIMi 610709. gene.
    neXtProti NX_Q9BXA7.
    PharmGKBi PA37944.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233016.
    HOVERGENi HBG101110.
    InParanoidi Q9BXA7.
    KOi K08811.
    OMAi WVQPKAR.
    OrthoDBi EOG7P8P8F.
    PhylomeDBi Q9BXA7.
    TreeFami TF352374.

    Enzyme and pathway databases

    SignaLinki Q9BXA7.

    Miscellaneous databases

    GenomeRNAii 83942.
    NextBioi 73081.
    PROi Q9BXA7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BXA7.
    Bgeei Q9BXA7.
    CleanExi HS_TSSK1B.
    Genevestigatori Q9BXA7.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. A TSSK member is present in the equatorial segment of human sperm."
      Hao Z., Jha K.N., Kim Y.H., Vemuganti S., Westbrook V.A., Chertihin O., Markgraf K., Flickinger C.J., Coppola M., Herr J.C., Visconti P.E.
      Mol. Hum. Reprod. 10:433-444(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH TSSK2.
    2. "Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate."
      Jaleel M., McBride A., Lizcano J.M., Deak M., Toth R., Morrice N.A., Alessi D.R.
      FEBS Lett. 579:1417-1423(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, AUTOPHOSPHORYLATION, PHOSPHORYLATION AT THR-174, MUTAGENESIS OF THR-174.
    3. "Molecular cloning and characterization of FKSG81, a novel gene located on human chromosome 5."
      Wang Y.-G., Gong L.
      Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    5. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Hippocampus.
    7. "Validation of a testis specific serine/threonine kinase [TSSK] family and the substrate of TSSK1 & 2, TSKS, as contraceptive targets."
      Xu B., Hao Z., Jha K.N., Digilio L., Urekar C., Kim Y.H., Pulido S., Flickinger C.J., Herr J.C.
      Soc. Reprod. Fertil. Suppl. 63:87-101(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PUTATIVE CONTRACEPTIVE TARGET.
    8. "Identification of peptide substrate and small molecule inhibitors of testis-specific serine/threonine kinase1 (TSSK1) by the developed assays."
      Zhang L., Yan Y., Liu Z., Abliz Z., Liu G.
      J. Med. Chem. 52:4419-4428(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION, FUNCTION.
    9. "Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis."
      Li Y., Sosnik J., Brassard L., Reese M., Spiridonov N.A., Bates T.C., Johnson G.R., Anguita J., Visconti P.E., Salicioni A.M.
      Mol. Hum. Reprod. 17:42-56(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    10. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-50; TYR-83; LEU-233; CYS-237; TRP-288 AND GLU-293.

    Entry informationi

    Entry nameiTSSK1_HUMAN
    AccessioniPrimary (citable) accession number: Q9BXA7
    Secondary accession number(s): B2R8D9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    TSSK1B might be used as a target for male contraception or and intra-vaginal spermicides.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3