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Q9BXA6

- TSSK6_HUMAN

UniProt

Q9BXA6 - TSSK6_HUMAN

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Protein

Testis-specific serine/threonine-protein kinase 6

Gene
TSSK6, SSTK, FKSG82
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for sperm production and function. Plays a role in DNA condensation during postmeiotic chromatin remodeling By similarity.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Magnesium.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATP1 Publication
Active sitei135 – 1351Proton acceptor1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATP By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein binding Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. protein phosphorylation Source: UniProtKB
  3. sperm chromatin condensation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
SignaLinkiQ9BXA6.

Names & Taxonomyi

Protein namesi
Recommended name:
Testis-specific serine/threonine-protein kinase 6 (EC:2.7.11.1)
Short name:
TSK-6
Short name:
TSSK-6
Short name:
Testis-specific kinase 6
Alternative name(s):
Cancer/testis antigen 72
Short name:
CT72
Serine/threonine-protein kinase SSTK
Small serine/threonine kinase
Gene namesi
Name:TSSK6
Synonyms:SSTK
ORF Names:FKSG82
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:30410. TSSK6.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProt
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi41 – 411K → M: Loss of kinase activity. Loss of binding to TSACC. 2 Publications
Mutagenesisi135 – 1351D → N: Loss of kinase activity. No effect of binding to TSACC. 2 Publications

Organism-specific databases

PharmGKBiPA142670682.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Testis-specific serine/threonine-protein kinase 6PRO_0000227746Add
BLAST

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9BXA6.
PRIDEiQ9BXA6.

PTM databases

PhosphoSiteiQ9BXA6.

Expressioni

Tissue specificityi

Highly expressed in testis. Expressed at lower levels in colon, small intestine, ovary, prostate, thymus, spleen and peripheral blood leukocytes.1 Publication

Gene expression databases

BgeeiQ9BXA6.
CleanExiHS_TSSK6.
GenevestigatoriQ9BXA6.

Interactioni

Subunit structurei

Interacts with the heat shock proteins HSPCB, HSPA8 and HSPA1A. These interactions appear to be required for TSSK6 kinase activity. Interacts with TSACC; this interaction is direct and recruits TSACC to HSP90, which is essential for kinase activity.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP90AB1P082383EBI-851883,EBI-352572

Protein-protein interaction databases

BioGridi123835. 9 interactions.
IntActiQ9BXA6. 3 interactions.
STRINGi9606.ENSP00000354168.

Structurei

3D structure databases

ProteinModelPortaliQ9BXA6.
SMRiQ9BXA6. Positions 12-270.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 267256Protein kinaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233016.
HOVERGENiHBG101110.
InParanoidiQ9BXA6.
KOiK08811.
OMAiVQRNGHI.
OrthoDBiEOG7P8P8F.
PhylomeDBiQ9BXA6.
TreeFamiTF105333.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BXA6-1 [UniParc]FASTAAdd to Basket

« Hide

MSGDKLLSEL GYKLGRTIGE GSYSKVKVAT SKKYKGTVAI KVVDRRRAPP    50
DFVNKFLPRE LSILRGVRHP HIVHVFEFIE VCNGKLYIVM EAAATDLLQA 100
VQRNGRIPGV QARDLFAQIA GAVRYLHDHH LVHRDLKCEN VLLSPDERRV 150
KLTDFGFGRQ AHGYPDLSTT YCGSAAYASP EVLLGIPYDP KKYDVWSMGV 200
VLYVMVTGCM PFDDSDIAGL PRRQKRGVLY PEGLELSERC KALIAELLQF 250
SPSARPSAGQ VARNCWLRAG DSG 273
Length:273
Mass (Da):30,331
Last modified:June 1, 2001 - v1
Checksum:i998934C72963C7CE
GO

Sequence cautioni

The sequence BAB71697.1 differs from that shown. Reason: Frameshift at positions 104 and 169.
The sequence BAD18805.1 differs from that shown. Reason: Frameshift at position 247.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF329483 mRNA. Translation: AAK48827.1.
AF348077 mRNA. Translation: AAK29414.1.
AK058166 mRNA. Translation: BAB71697.1. Frameshift.
AK172841 mRNA. Translation: BAD18805.1. Frameshift.
AK313767 mRNA. Translation: BAG36505.1.
CH471106 Genomic DNA. Translation: EAW84824.1.
BC014611 mRNA. Translation: AAH14611.1.
CCDSiCCDS12403.1.
RefSeqiNP_114426.1. NM_032037.3.
UniGeneiHs.532711.
Hs.666780.

Genome annotation databases

EnsembliENST00000360913; ENSP00000354168; ENSG00000178093.
ENST00000585580; ENSP00000466477; ENSG00000178093.
ENST00000587522; ENSP00000466056; ENSG00000178093.
GeneIDi83983.
KEGGihsa:83983.
UCSCiuc002nmr.3. human.

Polymorphism databases

DMDMi74761315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF329483 mRNA. Translation: AAK48827.1 .
AF348077 mRNA. Translation: AAK29414.1 .
AK058166 mRNA. Translation: BAB71697.1 . Frameshift.
AK172841 mRNA. Translation: BAD18805.1 . Frameshift.
AK313767 mRNA. Translation: BAG36505.1 .
CH471106 Genomic DNA. Translation: EAW84824.1 .
BC014611 mRNA. Translation: AAH14611.1 .
CCDSi CCDS12403.1.
RefSeqi NP_114426.1. NM_032037.3.
UniGenei Hs.532711.
Hs.666780.

3D structure databases

ProteinModelPortali Q9BXA6.
SMRi Q9BXA6. Positions 12-270.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123835. 9 interactions.
IntActi Q9BXA6. 3 interactions.
STRINGi 9606.ENSP00000354168.

PTM databases

PhosphoSitei Q9BXA6.

Polymorphism databases

DMDMi 74761315.

Proteomic databases

PaxDbi Q9BXA6.
PRIDEi Q9BXA6.

Protocols and materials databases

DNASUi 83983.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000360913 ; ENSP00000354168 ; ENSG00000178093 .
ENST00000585580 ; ENSP00000466477 ; ENSG00000178093 .
ENST00000587522 ; ENSP00000466056 ; ENSG00000178093 .
GeneIDi 83983.
KEGGi hsa:83983.
UCSCi uc002nmr.3. human.

Organism-specific databases

CTDi 83983.
GeneCardsi GC19M019623.
H-InvDB HIX0174374.
HGNCi HGNC:30410. TSSK6.
MIMi 610712. gene.
neXtProti NX_Q9BXA6.
PharmGKBi PA142670682.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233016.
HOVERGENi HBG101110.
InParanoidi Q9BXA6.
KOi K08811.
OMAi VQRNGHI.
OrthoDBi EOG7P8P8F.
PhylomeDBi Q9BXA6.
TreeFami TF105333.

Enzyme and pathway databases

BRENDAi 2.7.11.1. 2681.
SignaLinki Q9BXA6.

Miscellaneous databases

GenomeRNAii 83983.
NextBioi 73110.
PROi Q9BXA6.
SOURCEi Search...

Gene expression databases

Bgeei Q9BXA6.
CleanExi HS_TSSK6.
Genevestigatori Q9BXA6.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of SSTK, a serine/threonine protein kinase essential for male fertility."
    Spiridonov N.A., Wong L., Zerfas P.M., Starost M.F., Pack S.D., Paweletz C.P., Johnson G.R.
    Mol. Cell. Biol. 25:4250-4261(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH HSPCB; HSPA8 AND HSPA1A, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-41 AND ASP-135.
  2. "Identification of FKSG82, a novel gene located on human chromosome 19."
    Wang Y.-G., Gong L.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ileal mucosa and Testis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  6. "Identification of a novel HSP70-binding cochaperone critical to HSP90-mediated activation of small serine/threonine kinase."
    Jha K.N., Wong L., Zerfas P.M., De Silva R.S., Fan Y.X., Spiridonov N.A., Johnson G.R.
    J. Biol. Chem. 285:35180-35187(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, INTERACTION WITH TSACC, MUTAGENESIS OF LYS-41 AND ASP-135.

Entry informationi

Entry nameiTSSK6_HUMAN
AccessioniPrimary (citable) accession number: Q9BXA6
Secondary accession number(s): B2R9F8, Q6ZMC4, Q96LJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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