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Q9BXA6 (TSSK6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Testis-specific serine/threonine-protein kinase 6

Short name=TSK-6
Short name=TSSK-6
Short name=Testis-specific kinase 6
EC=2.7.11.1
Alternative name(s):
Cancer/testis antigen 72
Short name=CT72
Serine/threonine-protein kinase SSTK
Small serine/threonine kinase
Gene names
Name:TSSK6
Synonyms:SSTK
ORF Names:FKSG82
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for sperm production and function. Plays a role in DNA condensation during postmeiotic chromatin remodeling By similarity. Ref.1 UniProtKB Q925K9

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1 Ref.6

Cofactor

Magnesium. Ref.1

Subunit structure

Interacts with the heat shock proteins HSPCB, HSPA8 and HSPA1A. These interactions appear to be required for TSSK6 kinase activity. Interacts with TSACC; this interaction is direct and recruits TSACC to HSP90, which is essential for kinase activity. Ref.1 Ref.6

Tissue specificity

Highly expressed in testis. Expressed at lower levels in colon, small intestine, ovary, prostate, thymus, spleen and peripheral blood leukocytes. Ref.1

Post-translational modification

Autophosphorylated. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAB71697.1 differs from that shown. Reason: Frameshift at positions 104 and 169.

The sequence BAD18805.1 differs from that shown. Reason: Frameshift at position 247.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HSP90AB1P082383EBI-851883,EBI-352572

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273Testis-specific serine/threonine-protein kinase 6
PRO_0000227746

Regions

Domain12 – 267256Protein kinase
Nucleotide binding18 – 269ATP By similarity UniProtKB Q9D2E1

Sites

Active site1351Proton acceptor Ref.1
Binding site411ATP Ref.1

Experimental info

Mutagenesis411K → M: Loss of kinase activity. Loss of binding to TSACC. Ref.1 Ref.6
Mutagenesis1351D → N: Loss of kinase activity. No effect of binding to TSACC. Ref.1 Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q9BXA6 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 998934C72963C7CE

FASTA27330,331
        10         20         30         40         50         60 
MSGDKLLSEL GYKLGRTIGE GSYSKVKVAT SKKYKGTVAI KVVDRRRAPP DFVNKFLPRE 

        70         80         90        100        110        120 
LSILRGVRHP HIVHVFEFIE VCNGKLYIVM EAAATDLLQA VQRNGRIPGV QARDLFAQIA 

       130        140        150        160        170        180 
GAVRYLHDHH LVHRDLKCEN VLLSPDERRV KLTDFGFGRQ AHGYPDLSTT YCGSAAYASP 

       190        200        210        220        230        240 
EVLLGIPYDP KKYDVWSMGV VLYVMVTGCM PFDDSDIAGL PRRQKRGVLY PEGLELSERC 

       250        260        270 
KALIAELLQF SPSARPSAGQ VARNCWLRAG DSG 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of SSTK, a serine/threonine protein kinase essential for male fertility."
Spiridonov N.A., Wong L., Zerfas P.M., Starost M.F., Pack S.D., Paweletz C.P., Johnson G.R.
Mol. Cell. Biol. 25:4250-4261(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH HSPCB; HSPA8 AND HSPA1A, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-41 AND ASP-135.
[2]"Identification of FKSG82, a novel gene located on human chromosome 19."
Wang Y.-G., Gong L.
Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ileal mucosa and Testis.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[6]"Identification of a novel HSP70-binding cochaperone critical to HSP90-mediated activation of small serine/threonine kinase."
Jha K.N., Wong L., Zerfas P.M., De Silva R.S., Fan Y.X., Spiridonov N.A., Johnson G.R.
J. Biol. Chem. 285:35180-35187(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, INTERACTION WITH TSACC, MUTAGENESIS OF LYS-41 AND ASP-135.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF329483 mRNA. Translation: AAK48827.1.
AF348077 mRNA. Translation: AAK29414.1.
AK058166 mRNA. Translation: BAB71697.1. Frameshift.
AK172841 mRNA. Translation: BAD18805.1. Frameshift.
AK313767 mRNA. Translation: BAG36505.1.
CH471106 Genomic DNA. Translation: EAW84824.1.
BC014611 mRNA. Translation: AAH14611.1.
RefSeqNP_114426.1. NM_032037.3.
UniGeneHs.532711.
Hs.666780.

3D structure databases

ProteinModelPortalQ9BXA6.
SMRQ9BXA6. Positions 12-270.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123835. 9 interactions.
IntActQ9BXA6. 3 interactions.
STRING9606.ENSP00000354168.

PTM databases

PhosphoSiteQ9BXA6.

Polymorphism databases

DMDM74761315.

Proteomic databases

PaxDbQ9BXA6.
PRIDEQ9BXA6.

Protocols and materials databases

DNASU83983.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000360913; ENSP00000354168; ENSG00000178093.
ENST00000585580; ENSP00000466477; ENSG00000178093.
ENST00000587522; ENSP00000466056; ENSG00000178093.
GeneID83983.
KEGGhsa:83983.
UCSCuc002nmr.3. human.

Organism-specific databases

CTD83983.
GeneCardsGC19M019623.
H-InvDBHIX0174374.
HGNCHGNC:30410. TSSK6.
MIM610712. gene.
neXtProtNX_Q9BXA6.
PharmGKBPA142670682.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233016.
HOVERGENHBG101110.
InParanoidQ9BXA6.
KOK08811.
OMALELPERC.
OrthoDBEOG7P8P8F.
PhylomeDBQ9BXA6.
TreeFamTF105333.

Enzyme and pathway databases

BRENDA2.7.11.1. 2681.
SignaLinkQ9BXA6.

Gene expression databases

BgeeQ9BXA6.
CleanExHS_TSSK6.
GenevestigatorQ9BXA6.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi83983.
NextBio73110.
PROQ9BXA6.
SOURCESearch...

Entry information

Entry nameTSSK6_HUMAN
AccessionPrimary (citable) accession number: Q9BXA6
Secondary accession number(s): B2R9F8, Q6ZMC4, Q96LJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM