Q9BXA6 (TSSK6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Testis-specific serine/threonine-protein kinase 6
Short name=TSK-6
Short name=TSSK-6
Short name=Testis-specific kinase 6
EC=2.7.11.1

Alternative name(s):
Cancer/testis antigen 72
Short name=CT72
Serine/threonine-protein kinase SSTK
Small serine/threonine kinase

Gene names

Name:	TSSK6
Synonyms:	SSTK
ORF Names:	FKSG82

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for sperm production and function. Plays a role in DNA condensation during postmeiotic chromatin remodeling By similarity. Ref.1 UniProtKB Q925K9
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.1 Ref.6
Cofactor	Magnesium. Ref.1
Subunit structure	Interacts with the heat shock proteins HSPCB, HSPA8 and HSPA1A. These interactions appear to be required for TSSK6 kinase activity. Interacts with TSACC; this interaction is direct and recruits TSACC to HSP90, which is essential for kinase activity. Ref.1 Ref.6
Tissue specificity	Highly expressed in testis. Expressed at lower levels in colon, small intestine, ovary, prostate, thymus, spleen and peripheral blood leukocytes. Ref.1
Post-translational modification	Autophosphorylated. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence BAB71697.1 differs from that shown. Reason: Frameshift at positions 104 and 169. The sequence BAD18805.1 differs from that shown. Reason: Frameshift at position 247.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW sperm chromatin condensation Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from direct assay Ref.1. Source: UniProtKB magnesium ion binding Inferred from direct assay Ref.1. Source: UniProtKB protein serine/threonine kinase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HSP90AB1	P08238	3	EBI-851883,EBI-352572

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Testis-specific serine/threonine-protein kinase 6		PRO_0000227746
Regions
	Domain	12 – 267	256	Protein kinase
	Nucleotide binding	18 – 26	9	ATP By similarity UniProtKB Q9D2E1
Sites
	Active site	135	1	Proton acceptor Ref.1
	Binding site	41	1	ATP Ref.1
Experimental info
	Mutagenesis	41	1	K → M: Loss of kinase activity. Loss of binding to TSACC. Ref.1 Ref.6
	Mutagenesis	135	1	D → N: Loss of kinase activity. No effect of binding to TSACC. Ref.1 Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9BXA6 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 998934C72963C7CE	FASTA	273	30,331
	10 20 30 40 50 60 MSGDKLLSEL GYKLGRTIGE GSYSKVKVAT SKKYKGTVAI KVVDRRRAPP DFVNKFLPRE 70 80 90 100 110 120 LSILRGVRHP HIVHVFEFIE VCNGKLYIVM EAAATDLLQA VQRNGRIPGV QARDLFAQIA 130 140 150 160 170 180 GAVRYLHDHH LVHRDLKCEN VLLSPDERRV KLTDFGFGRQ AHGYPDLSTT YCGSAAYASP 190 200 210 220 230 240 EVLLGIPYDP KKYDVWSMGV VLYVMVTGCM PFDDSDIAGL PRRQKRGVLY PEGLELSERC 250 260 270 KALIAELLQF SPSARPSAGQ VARNCWLRAG DSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of SSTK, a serine/threonine protein kinase essential for male fertility." Spiridonov N.A., Wong L., Zerfas P.M., Starost M.F., Pack S.D., Paweletz C.P., Johnson G.R. Mol. Cell. Biol. 25:4250-4261(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH HSPCB; HSPA8 AND HSPA1A, AUTOPHOSPHORYLATION, MUTAGENESIS OF LYS-41 AND ASP-135.
[2]	"Identification of FKSG82, a novel gene located on human chromosome 19." Wang Y.-G., Gong L. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ileal mucosa and Testis.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[6]	"Identification of a novel HSP70-binding cochaperone critical to HSP90-mediated activation of small serine/threonine kinase." Jha K.N., Wong L., Zerfas P.M., De Silva R.S., Fan Y.X., Spiridonov N.A., Johnson G.R. J. Biol. Chem. 285:35180-35187(2010) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, INTERACTION WITH TSACC, MUTAGENESIS OF LYS-41 AND ASP-135.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF329483 mRNA. Translation: AAK48827.1. AF348077 mRNA. Translation: AAK29414.1. AK058166 mRNA. Translation: BAB71697.1. Frameshift. AK172841 mRNA. Translation: BAD18805.1. Frameshift. AK313767 mRNA. Translation: BAG36505.1. CH471106 Genomic DNA. Translation: EAW84824.1. BC014611 mRNA. Translation: AAH14611.1.
IPI	IPI00012485.
RefSeq	NP_114426.1. NM_032037.3.
UniGene	Hs.532711. Hs.666780.
3D structure databases
ProteinModelPortal	Q9BXA6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BXA6. 3 interactions.
STRING	9606.ENSP00000354168.
PTM databases
PhosphoSite	Q9BXA6.
Polymorphism databases
DMDM	74761315.
Proteomic databases
PaxDb	Q9BXA6.
PRIDE	Q9BXA6.
Protocols and materials databases
DNASU	83983.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000360913; ENSP00000354168; ENSG00000178093. ENST00000585580; ENSP00000466477; ENSG00000178093. ENST00000587522; ENSP00000466056; ENSG00000178093.
GeneID	83983.
KEGG	hsa:83983.
UCSC	uc002nmr.3. human.
Organism-specific databases
CTD	83983.
GeneCards	GC19M019623.
H-InvDB	HIX0174374.
HGNC	HGNC:30410. TSSK6.
MIM	610712. gene.
neXtProt	NX_Q9BXA6.
PharmGKB	PA142670682.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233016.
HOVERGEN	HBG101110.
InParanoid	Q9BXA6.
KO	K08811.
OMA	LELPERC.
OrthoDB	EOG415GF0.
PhylomeDB	Q9BXA6.
Enzyme and pathway databases
BRENDA	2.7.11.1. 2681.
Gene expression databases
Bgee	Q9BXA6.
CleanEx	HS_TSSK6.
Genevestigator	Q9BXA6.
GermOnline	ENSG00000178093. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	83983.
NextBio	73110.
SOURCE	Search...

Entry information

Entry name

TSSK6_HUMAN

Accession

Primary (citable) accession number: Q9BXA6
Secondary accession number(s): B2R9F8, Q6ZMC4, Q96LJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.