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Protein

Sphingosine-1-phosphate phosphatase 1

Gene

SGPP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phoshate, lysophosphatidic acid or phosphatidic acid. Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger (PubMed:12815058, PubMed:11756451). Involved in efficient ceramide synthesis from exogenous sphingoid bases. Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase. In concert with sphingosine kinase 2 (SphK2), recycles sphingosine into ceramide though a phosphorylation/dephosphorylation cycle. Regulates intracellular ceramide levels, which in turn regulate apoptosis (By similarity). Via S1P levels, modulates resting tone, intracellular Ca2+ and myogenic vasoconstriction in resistance arteries (PubMed:18583713). Also involved in unfolded protein response (UPR) and ER stress-induced autophagy via regulation of intracellular S1P levels (PubMed:20798685).By similarity4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei208Proton donorBy similarity1
Active sitei255NucleophileBy similarity1
Sitei259Stabilizes the active site histidine for nucleophilic attackBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-HSA-1660661. Sphingolipid de novo biosynthesis.

Chemistry databases

SwissLipidsiSLP:000000158.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine-1-phosphate phosphatase 1 (EC:3.1.3.-)
Short name:
SPPase1
Short name:
Spp1
Short name:
hSPP1
Short name:
hSPPase1
Alternative name(s):
Sphingosine-1-phosphatase 1
Gene namesi
Name:SGPP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000126821.7.
HGNCiHGNC:17720. SGPP1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Transmembranei163 – 183HelicalSequence analysisAdd BLAST21
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Transmembranei227 – 247HelicalSequence analysisAdd BLAST21
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Transmembranei290 – 310HelicalSequence analysisAdd BLAST21
Transmembranei322 – 342HelicalSequence analysisAdd BLAST21
Transmembranei359 – 379HelicalSequence analysisAdd BLAST21
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi208H → A: Abolishes phosphatase activity. 1 Publication1

Organism-specific databases

DisGeNETi81537.
OpenTargetsiENSG00000126821.
PharmGKBiPA134884424.

Polymorphism and mutation databases

BioMutaiSGPP1.
DMDMi46577706.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001144771 – 441Sphingosine-1-phosphate phosphatase 1Add BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101PhosphoserineCombined sources1
Modified residuei112PhosphoserineCombined sources1
Modified residuei114PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BX95.
MaxQBiQ9BX95.
PaxDbiQ9BX95.
PeptideAtlasiQ9BX95.
PRIDEiQ9BX95.

PTM databases

DEPODiQ9BX95.
iPTMnetiQ9BX95.
PhosphoSitePlusiQ9BX95.

Expressioni

Tissue specificityi

Ubiquitous, with the strongest level in placenta and kidney.1 Publication

Gene expression databases

BgeeiENSG00000126821.
CleanExiHS_SGPP1.
GenevisibleiQ9BX95. HS.

Organism-specific databases

HPAiHPA053149.
HPA064908.

Interactioni

Protein-protein interaction databases

BioGridi123508. 30 interactors.
IntActiQ9BX95. 3 interactors.
STRINGi9606.ENSP00000247225.

Structurei

3D structure databases

ProteinModelPortaliQ9BX95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni178 – 186Phosphatase sequence motif ICurated9
Regioni205 – 208Phosphatase sequence motif IICurated4
Regioni248 – 259Phosphatase sequence motif IIICuratedAdd BLAST12

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2822. Eukaryota.
COG0671. LUCA.
GeneTreeiENSGT00390000017322.
HOGENOMiHOG000234228.
HOVERGENiHBG079185.
InParanoidiQ9BX95.
KOiK04716.
OMAiFYILFFP.
OrthoDBiEOG091G092E.
PhylomeDBiQ9BX95.
TreeFamiTF323419.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiView protein in InterPro
IPR000326. P_Acid_Pase_2/haloperoxidase.
PfamiView protein in Pfam
PF01569. PAP2. 1 hit.
SMARTiView protein in SMART
SM00014. acidPPc. 1 hit.
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BX95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRQRLAQL VGRLQDPQKV ARFQRLCGVE APPRRSADRR EDEKAEAPLA
60 70 80 90 100
GDPRLRGRQP GAPGGPQPPG SDRNQCPAKP DGGGAPNGVR NGLAAELGPA
110 120 130 140 150
SPRRAGALRR NSLTGEEGQL ARVSNWPLYC LFCFGTELGN ELFYILFFPF
160 170 180 190 200
WIWNLDPLVG RRLVVIWVLV MYLGQCTKDI IRWPRPASPP VVKLEVFYNS
210 220 230 240 250
EYSMPSTHAM SGTAIPISMV LLTYGRWQYP LIYGLILIPC WCSLVCLSRI
260 270 280 290 300
YMGMHSILDI IAGFLYTILI LAVFYPFVDL IDNFNQTHKY APFIIIGLHL
310 320 330 340 350
ALGIFSFTLD TWSTSRGDTA EILGSGAGIA CGSHVTYNMG LVLDPSLDTL
360 370 380 390 400
PLAGPPITVT LFGKAILRIL IGMVFVLIIR DVMKKITIPL ACKIFNIPCD
410 420 430 440
DIRKARQHME VELPYRYITY GMVGFSITFF VPYIFFFIGI S
Length:441
Mass (Da):49,108
Last modified:April 26, 2004 - v2
Checksum:i86A018DDDA3A3019
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146L → P in AAK26660 (PubMed:12815058).Curated1
Sequence conflicti191V → A in AAK26660 (PubMed:12815058).Curated1
Sequence conflicti282D → G in AAK26660 (PubMed:12815058).Curated1
Sequence conflicti320A → T in AAK26660 (PubMed:12815058).Curated1
Sequence conflicti371I → V in AAK26660 (PubMed:12815058).Curated1
Sequence conflicti375F → S in AAK26660 (PubMed:12815058).Curated1
Sequence conflicti410E → G in AAK26660 (PubMed:12815058).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF349315 mRNA. Translation: AAK26660.1.
AJ293294 mRNA. Translation: CAC17772.1.
AK314188 mRNA. Translation: BAG36867.1.
AL161670 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW80834.1.
BC063839 mRNA. Translation: AAH63839.1.
BC070060 mRNA. Translation: AAH70060.1.
CCDSiCCDS9760.1.
RefSeqiNP_110418.1. NM_030791.3.
UniGeneiHs.24678.

Genome annotation databases

EnsembliENST00000247225; ENSP00000247225; ENSG00000126821.
GeneIDi81537.
KEGGihsa:81537.
UCSCiuc001xgj.5. human.

Similar proteinsi

Entry informationi

Entry nameiSGPP1_HUMAN
AccessioniPrimary (citable) accession number: Q9BX95
Secondary accession number(s): B2RAH0, Q9H189
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: April 26, 2004
Last modified: September 27, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families