Q9BX70 (BTBD2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
BTB/POZ domain-containing protein 2

Gene names

Name:

BTBD2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with topoisomerase 1 and with TRIM5 isoform Delta. Ref.1 Ref.7
Subcellular location	Cytoplasm › P-body. Note: Cytoplasmic bodies. Ref.7
Sequence similarities	Contains 1 BTB (POZ) domain.
Sequence caution	The sequence AAC16070.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB55143.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasmic mRNA processing body Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
COPS6	Q7L5N1	2	EBI-710091,EBI-486838
TP53	P04637	2	EBI-710091,EBI-366083

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BX70-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BX70-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-321: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 525

525

BTB/POZ domain-containing protein 2

PRO_0000186210

Regions

Domain

117 – 187

71

BTB

Compositional bias

41 – 54

14

Poly-Ala

Natural variations

Alternative sequence

1 – 321

321

Missing in isoform 2.

VSP_010562

Experimental info

Sequence conflict

307

1

R → L in CAB96527. Ref.6

Secondary structure

1

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525

Helix Strand Turn

Details...

Beta strand

372 – 375

4

Beta strand

378 – 384

7

Beta strand

391 – 399

9

Beta strand

401 – 409

9

Beta strand

411 – 426

16

Turn

427 – 430

4

Beta strand

431 – 442

12

Beta strand

448 – 459

12

Beta strand

465 – 474

10

Beta strand

476 – 481

6

Beta strand

484 – 489

6

Turn

491 – 493

3

Beta strand

496 – 502

7

Beta strand

506 – 508

3

Beta strand

513 – 517

5

Beta strand

520 – 525

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 5F4856161702A65D	FASTA	525	55,931
	10 20 30 40 50 60 MAAGGSGGRA SCPPGVGVGP GTGGSPGPSA NAAATPAPGN AAAAAAAAAA AAAAPGPTPP 70 80 90 100 110 120 APPGPGTDAQ AAGAERAEEA AGPGAAALQR EAAYNWQASK PTVQERFAFL FNNEVLCDVH 130 140 150 160 170 180 FLVGKGLSSQ RIPAHRFVLA VGSAVFDAMF NGGMATTSTE IELPDVEPAA FLALLKFLYS 190 200 210 220 230 240 DEVQIGPETV MTTLYTAKKY AVPALEAHCV EFLKKNLRAD NAFMLLTQAR LFDEPQLASL 250 260 270 280 290 300 CLENIDKNTA DAITAEGFTD IDLDTLVAVL ERDTLGIREV RLFNAVVRWS EAECQRQQLQ 310 320 330 340 350 360 VTPENRRKVL GKALGLIRFP LMTIEEFAAG PAQSGILVDR EVVSLFLHFT VNPKPRVEFI 370 380 390 400 410 420 DRPRCCLRGK ECSINRFQQV ESRWGYSGTS DRIRFSVNKR IFVVGFGLYG SIHGPTDYQV 430 440 450 460 470 480 NIQIIHTDSN TVLGQNDTGF SCDGSASTFR VMFKEPVEVL PNVNYTACAT LKGPDSHYGT 490 500 510 520 KGLRKVTHES PTTGAKTCFT FCYAAGNNNG TSVEDGQIPE VIFYT
	Isoform 2 [UniParc]. Checksum: 08727FE5DF916D4C	FASTA	204	22,566
	10 20 30 40 50 60 MTIEEFAAGP AQSGILVDRE VVSLFLHFTV NPKPRVEFID RPRCCLRGKE CSINRFQQVE 70 80 90 100 110 120 SRWGYSGTSD RIRFSVNKRI FVVGFGLYGS IHGPTDYQVN IQIIHTDSNT VLGQNDTGFS 130 140 150 160 170 180 CDGSASTFRV MFKEPVEVLP NVNYTACATL KGPDSHYGTK GLRKVTHESP TTGAKTCFTF 190 200 CYAAGNNNGT SVEDGQIPEV IFYT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of BTBD1 and BTBD2, two similar BTB-domain-containing Kelch-like proteins that interact with topoisomerase I." Xu L., Yang L., Hashimoto K., Anderson M., Kohlhagen G., Pommier Y., D'Arpa P. BMC Genomics 3:1-1(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH TOP1, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 214-525 (ISOFORM 1). Tissue: Teratocarcinoma.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-525 (ISOFORM 1). Tissue: Blood, Brain, Colon and Ovary.
[6]	The European IMAGE consortium Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 258-525 (ISOFORM 1).
[7]	"BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta." Xu L., Yang L., Moitra P.K., Hashimoto K., Rallabhandi P., Kaul S., Meroni G., Jensen J.P., Weissman A.M., D'Arpa P. Exp. Cell Res. 288:84-93(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH TRIM5.
[8]	"Crystal structure of the PHR domain from human BTBD2 protein." New York structural genomix research consortium (NYSGXRC) Submitted (AUG-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 361-525.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF355797 mRNA. Translation: AAK25826.1.
AK000393 mRNA. Translation: BAA91136.1.
AK027481 mRNA. Translation: BAB55143.1. Different initiation.
CR457230 mRNA. Translation: CAG33511.1.
AC004678 Genomic DNA. Translation: AAC16070.1. Sequence problems.
AC005306 Genomic DNA. Translation: AAC26984.1.
AC005306 Genomic DNA. Translation: AAG45443.1.
BC000564 mRNA. Translation: AAH00564.2.
BC008035 mRNA. Translation: AAH08035.2.
BC053986 mRNA. Translation: AAH53986.1.
BC094820 mRNA. Translation: AAH94820.1.
AL360275 mRNA. Translation: CAB96527.1.

IPI

IPI00022826.
IPI00413200.

RefSeq

NP_060267.2. NM_017797.3.

UniGene

Hs.465543.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3NO8	X-ray	2.20	A/B	361-525	[»]

ProteinModelPortal

Q9BX70.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9BX70. 27 interactions.

MINT

MINT-1185053.

PTM databases

PhosphoSite

Q9BX70.

Polymorphism databases

DMDM

20137455.

Proteomic databases

PaxDb

Q9BX70.

PRIDE

Q9BX70.

Protocols and materials databases

DNASU

55643.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000255608; ENSP00000255608; ENSG00000133243.

GeneID

55643.

KEGG

hsa:55643.

UCSC

uc002luo.1. human.
uc002lup.1. human.

Organism-specific databases

CTD

55643.

GeneCards

GC19M001985.

H-InvDB

HIX0040331.

HGNC

HGNC:15504. BTBD2.

MIM

608531. gene.

neXtProt

NX_Q9BX70.

PharmGKB

PA25439.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG308618.

HOVERGEN

HBG050743.

InParanoid

Q9BX70.

KO

K10477.

OMA

CDGSANT.

OrthoDB

EOG480HWK.

PhylomeDB

Q9BX70.

Gene expression databases

ArrayExpress

Q9BX70.

Bgee

Q9BX70.

Genevestigator

Q9BX70.

GermOnline

ENSG00000133243. Homo sapiens.

Family and domain databases

Gene3D

3.30.710.10. 1 hit.

InterPro

IPR011705. BACK.
IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR012983. PHR.
[Graphical view]

Pfam

PF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF08005. PHR. 1 hit.
[Graphical view]

SMART

SM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
[Graphical view]

SUPFAM

SSF54695. BTB/POZ_fold. 1 hit.

PROSITE

PS50097. BTB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9BX70.

GenomeRNAi

55643.

NextBio

60330.

SOURCE

Search...

Entry information

Entry name

BTBD2_HUMAN

Accession

Primary (citable) accession number: Q9BX70
Secondary accession number(s): O60418 , O75248, Q4VBZ1, Q6IAC5, Q7Z5W0, Q96SX8, Q9NPS1, Q9NX81

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.