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Q9BX68 (HINT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histidine triad nucleotide-binding protein 2, mitochondrial

Short name=HINT-2
EC=3.-.-.-
Alternative name(s):
HINT-3
HIT-17kDa
PKCI-1-related HIT protein
Gene names
Name:HINT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length163 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolase probably involved in steroid biosynthesis. May play a role in apoptosis. Has adenosine phosphoramidase activity. Ref.8 Ref.9

Subcellular location

Mitochondrion Ref.8 Ref.9.

Tissue specificity

High expression in liver and pancreas. Expression is significantly down-regulated in hepatocellular carcinoma (HCC) patients. Ref.8

Sequence similarities

Belongs to the HINT family.

Contains 1 HIT domain.

Biophysicochemical properties

Kinetic parameters:

KM=128 µM for adenosine 5'-O-p-nitrophenylphosphoramidate Ref.8

Ontologies

Keywords
   Biological processApoptosis
Lipid biosynthesis
Lipid metabolism
Steroid biosynthesis
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandNucleotide-binding
   Molecular functionHydrolase
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from electronic annotation. Source: UniProtKB-KW

steroid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrion

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionhydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotide binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1717Mitochondrion Potential
Chain18 – 163146Histidine triad nucleotide-binding protein 2, mitochondrial
PRO_0000109786

Regions

Domain55 – 163109HIT
Nucleotide binding80 – 812Purine nucleotide phosphoramidate By similarity
Nucleotide binding142 – 1443Purine nucleotide phosphoramidate By similarity
Nucleotide binding149 – 1513Purine nucleotide phosphoramidate By similarity
Motif147 – 1515Histidine triad motif

Sites

Active site1491Tele-AMP-histidine intermediate Ref.8
Binding site1361Purine nucleotide phosphoramidate By similarity

Amino acid modifications

Modified residue1191N6-acetyllysine By similarity
Modified residue1391N6-acetyllysine By similarity

Experimental info

Mutagenesis1491H → A: Loss of adenosine phosphoramidase activity. Ref.8

Secondary structure

...................... 163
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9BX68 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 63A0C15D13749F99

FASTA16317,162
        10         20         30         40         50         60 
MAAAVVLAAG LRAARRAVAA TGVRGGQVRG AAGVTDGNEV AKAQQATPGG AAPTIFSRIL 

        70         80         90        100        110        120 
DKSLPADILY EDQQCLVFRD VAPQAPVHFL VIPKKPIPRI SQAEEEDQQL LGHLLLVAKQ 

       130        140        150        160 
TAKAEGLGDG YRLVINDGKL GAQSVYHLHI HVLGGRQLQW PPG 

« Hide

References

« Hide 'large scale' references
[1]"HIT-17kDa is a new protein from the HIT family with high homology to PKCI-1."
Magnino F., Dufour J.-F.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"PKCI-1-related HIT protein."
Takada F., Lugus J.J., Beggs A.H.
Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Hint-2 genomic sequence."
Takada F., Lugus J.J., Beggs A.H.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"HINT-3 is a novel member of the histidine triad protein family."
Chen H., Peng J., Huang C.-H.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[5]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas.
[8]"Hint2, a mitochondrial apoptotic sensitizer down-regulated in hepatocellular carcinoma."
Martin J., Magnino F., Schmidt K., Piguet A.-C., Lee J.S., Semela D., St-Pierre M.V., Ziemiecki A., Cassio D., Brenner C., Thorgeirsson S.S., Dufour J.-F.
Gastroenterology 130:2179-2188(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ACTIVE SITE, MUTAGENESIS OF HIS-149.
[9]"Hint2 is expressed in the mitochondria of H295R cells and is involved in steroidogenesis."
Lenglet S., Antigny F., Vetterli L., Dufour J.-F., Rossier M.F.
Endocrinology 149:5461-5469(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF356515 mRNA. Translation: AAK37562.1.
AY033094 mRNA. Translation: AAK53455.1.
AF490476 Genomic DNA. Translation: AAM09526.1.
AF356875 mRNA. Translation: AAM00221.1.
AL133410 Genomic DNA. Translation: CAI10991.1.
CH471071 Genomic DNA. Translation: EAW58332.1.
BC047737 mRNA. Translation: AAH47737.1.
CCDSCCDS6594.1.
RefSeqNP_115982.1. NM_032593.2.
UniGeneHs.70573.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4INCX-ray1.19A/B37-163[»]
4INIX-ray1.65A/B37-163[»]
4NJXX-ray2.83A/B/C/D1-163[»]
4NJYX-ray1.32A1-163[»]
4NJZX-ray2.40A/B/C/D1-163[»]
4NK0X-ray2.02A/B1-163[»]
ProteinModelPortalQ9BX68.
SMRQ9BX68. Positions 52-163.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124200. 2 interactions.
STRING9606.ENSP00000259667.

PTM databases

PhosphoSiteQ9BX68.

Polymorphism databases

DMDM51701612.

2D gel databases

OGPQ9BX68.

Proteomic databases

MaxQBQ9BX68.
PaxDbQ9BX68.
PeptideAtlasQ9BX68.
PRIDEQ9BX68.

Protocols and materials databases

DNASU84681.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000259667; ENSP00000259667; ENSG00000137133.
GeneID84681.
KEGGhsa:84681.
UCSCuc003zyh.3. human.

Organism-specific databases

CTD84681.
GeneCardsGC09M035802.
HGNCHGNC:18344. HINT2.
HPAHPA020961.
MIM609997. gene.
neXtProtNX_Q9BX68.
PharmGKBPA29287.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0537.
HOGENOMHOG000061064.
HOVERGENHBG051906.
InParanoidQ9BX68.
OMADRELVGH.
OrthoDBEOG75QR5T.
PhylomeDBQ9BX68.
TreeFamTF314862.

Gene expression databases

BgeeQ9BX68.
CleanExHS_HINT2.
GenevestigatorQ9BX68.

Family and domain databases

Gene3D3.30.428.10. 1 hit.
InterProIPR019808. Histidine_triad_CS.
IPR001310. Histidine_triad_HIT.
IPR011146. HIT-like.
[Graphical view]
PANTHERPTHR23089. PTHR23089. 1 hit.
PfamPF01230. HIT. 1 hit.
[Graphical view]
PRINTSPR00332. HISTRIAD.
SUPFAMSSF54197. SSF54197. 1 hit.
PROSITEPS00892. HIT_1. 1 hit.
PS51084. HIT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi84681.
NextBio74724.
PROQ9BX68.
SOURCESearch...

Entry information

Entry nameHINT2_HUMAN
AccessionPrimary (citable) accession number: Q9BX68
Secondary accession number(s): Q5TCW3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM