Reviewed, UniProtKB/Swiss-Prot Q9BX67 (JAM3_HUMAN)

Names and origin

Protein names

Recommended name:
    Junctional adhesion molecule C
      Short name=JAM-C
Alternative name(s):
    Junctional adhesion molecule 3
      Short name=JAM-3
    JAM-2

Gene names

Name:	JAM3
ORF Names:	UNQ859/PRO1868

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May participate in cell-cell adhesion distinct from tight junctions.
Subunit structure	Interacts with JAM2. Ref.1
Subcellular location	Cell membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Widely expressed. Highest expression in placenta, brain and kidney. Ref.1 Ref.4
Sequence similarities	Belongs to the immunoglobulin superfamily. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Immunoglobulin domain Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Ref.9
	Chain	32 – 310	279	Junctional adhesion molecule C		PRO_0000015071
Regions
	Topological domain	32 – 241	210	Extracellular Potential
	Transmembrane	242 – 262	21	Potential
	Topological domain	263 – 310	48	Cytoplasmic Potential
	Domain	35 – 127	93	Ig-like V-type
	Domain	139 – 236	98	Ig-like C2-type
Amino acid modifications
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Ref.12
	Glycosylation	198	1	N-linked (GlcNAc...) Ref.12
	Disulfide bond	53 ↔ 115		Potential
	Disulfide bond	160 ↔ 219		Potential
Experimental info
	Sequence conflict	136	1	Q → R in AAH10690. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q9BX67-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: CE39ADF33EA1DAB9	FASTA	310	35,020
	10 20 30 40 50 60 MALRRPPRLR LCARLPDFFL LLLFRGCLIG AVNLKSSNRT PVVQEFESVE LSCIITDSQT 70 80 90 100 110 120 SDPRIEWKKI QDEQTTYVFF DNKIQGDLAG RAEILGKTSL KIWNVTRRDS ALYRCEVVAR 130 140 150 160 170 180 NDRKEIDEIV IELTVQVKPV TPVCRVPKAV PVGKMATLHC QESEGHPRPH YSWYRNDVPL 190 200 210 220 230 240 PTDSRANPRF RNSSFHLNSE TGTLVFTAVH KDDSGQYYCI ASNDAGSARC EEQEMEVYDL 250 260 270 280 290 300 NIGGIIGGVL VVLAVLALIT LGICCAYRRG YFINNKQDGE SYKNPGKPDG VNYIRTDEEG 310 DFRHKSSFVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor." Arrate M.P., Rodriguez J.M., Tran T.M., Brock T.A., Cunningham S.A. J. Biol. Chem. 276:45826-45832(2001) [PubMed: 11590146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH JAM2, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members." Aurrand-Lions M.A., Johnson-Leger C., Wong C., Du Pasquier L., Imhof B.A. Blood 98:3699-3707(2001) [PubMed: 11739175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The junctional adhesion molecule 3 (JAM-3) on human platelets is a counterreceptor for the leukocyte integrin Mac-1." Santoso S., Sachs U.J.H., Kroll H., Linder M., Ruf A., Preissner K.T., Chavakis T. J. Exp. Med. 196:679-691(2002) [PubMed: 12208882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Narrowing the critical region within 11q24-qter for hypoplastic left heart and identification of a candidate gene, JAM3, expressed during cardiogenesis." Phillips H.M., Renforth G.L., Spalluto C., Hearn T., Curtis A.R.J., Craven L., Havarani B., Clement-Jones M., English C., Stumper O., Salmon T., Hutchinson S., Jackson M.S., Wilson D.I. Genomics 79:475-478(2002) [PubMed: 11944976] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye and Uterus.
[9]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-46.
[10]	"Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response." Muller W.A. Trends Immunol. 24:327-334(2003) [PubMed: 12810109] [Abstract] Cited for: REVIEW, NOMENCLATURE.
[11]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[12]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-192 AND ASN-198, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF356518 mRNA. Translation: AAK27221.1. AJ344431 mRNA. Translation: CAC69845.1. AF448478 mRNA. Translation: AAM20925.1. AJ416101 mRNA. Translation: CAC94776.1. Different initiation. AK074769 mRNA. Translation: BAC11195.1. AK075309 mRNA. Translation: BAC11538.1. AY358335 mRNA. Translation: AAQ88701.1. AP000911 Genomic DNA. No translation available. AP001775 Genomic DNA. No translation available. BC010690 mRNA. Translation: AAH10690.1. BC012147 mRNA. Translation: AAH12147.1.
IPI	IPI00152850.
RefSeq	NP_116190.2.
UniGene	Hs.150718 Hs.700882
3D structure databases
HSSP	HSSP built from PDB template 1F97 based on UniProtKB O88792.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9BX67.
Proteomic databases
PRIDE	Q9BX67.
Genome annotation databases
Ensembl	ENST00000299106; ENSP00000299106; ENSG00000166086; Homo sapiens. [Genome view] ENST00000441717; ENSP00000395742; ENSG00000166086; Homo sapiens. [Genome view]
GeneID	83700.
KEGG	hsa:83700.
UCSC	uc001qhb.1. human.
Organism-specific databases
CTD	83700.
GeneCards	GC11P133444.
H-InvDB	HIX0010295.
HGNC	HGNC:15532. JAM3.
MIM	606871. gene.
PharmGKB	PA29993.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9BX67.
HOVERGEN	Q9BX67.
Enzyme and pathway databases
Pathway_Interaction_DB	amb2_neutrophils_pathway. amb2 Integrin signaling.
Reactome	REACT_13552. Integrin cell surface interactions. REACT_604. Hemostasis. REACT_6900. Signaling in Immune system.
Gene expression databases
ArrayExpress	Q9BX67.
Bgee	Q9BX67.
CleanEx	HS_JAM3.
Genevestigator	Q9BX67.
GermOnline	ENSG00000166086. Homo sapiens.
Family and domain databases
InterPro	IPR013151. Ig. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR013106. Ig_V-set. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
Pfam	PF00047. ig. 1 hit. PF07686. V-set. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. SM00408. IGc2. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	72688.
SOURCE	Search...

Entry information

Entry name

JAM3_HUMAN

Accession

Primary (citable) accession number: Q9BX67
Secondary accession number(s): Q8WWL8, Q96FL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 1, 2001
Last modified:	October 13, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.