ID   SCRT1_HUMAN             Reviewed;         348 AA.
AC   Q9BWW7; A8MX66; Q96C52;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-FEB-2022, sequence version 2.
DT   24-JAN-2024, entry version 158.
DE   RecName: Full=Transcriptional repressor scratch 1;
DE   AltName: Full=Scratch homolog 1 zinc finger protein;
DE            Short=SCRT;
DE            Short=Scratch 1;
DE            Short=hScrt;
GN   Name=SCRT1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, INTERACTION WITH E-BOX MOTIF, AND VARIANT SER-133.
RC   TISSUE=Brain;
RX   PubMed=11274425; DOI=10.1073/pnas.051014098;
RA   Nakakura E.K., Watkins D.N., Schuebel K.E., Sriuranpong V., Borges M.W.,
RA   Nelkin B.D., Ball D.W.;
RT   "Mammalian scratch: a neural-specific snail family transcriptional
RT   repressor.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:4010-4015(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 185-348.
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Transcriptional repressor that binds E-box motif CAGGTG. Can
CC       modulate the action of basic helix-loop-helix (bHLH) transcription
CC       factors, critical for neuronal differentiation.
CC       {ECO:0000269|PubMed:11274425}.
CC   -!- SUBUNIT: Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP
CC       (via LIM domains) and AJUBA (via LIM domains). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11274425}.
CC   -!- TISSUE SPECIFICITY: Brain specific. {ECO:0000269|PubMed:11274425}.
CC   -!- DOMAIN: The N-terminal non zinc-finger region mediates the repressor
CC       activity.
CC   -!- SIMILARITY: Belongs to the snail C2H2-type zinc-finger protein family.
CC       {ECO:0000305}.
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DR   EMBL; AY014996; AAK01467.1; -; mRNA.
DR   EMBL; CH471162; EAW82125.1; -; Genomic_DNA.
DR   EMBL; BC014675; AAH14675.1; -; mRNA.
DR   CCDS; CCDS6421.1; -.
DR   RefSeq; NP_112599.2; NM_031309.5.
DR   AlphaFoldDB; Q9BWW7; -.
DR   SMR; Q9BWW7; -.
DR   BioGRID; 123667; 26.
DR   STRING; 9606.ENSP00000455711; -.
DR   iPTMnet; Q9BWW7; -.
DR   PhosphoSitePlus; Q9BWW7; -.
DR   BioMuta; SCRT1; -.
DR   DMDM; 46397014; -.
DR   jPOST; Q9BWW7; -.
DR   MassIVE; Q9BWW7; -.
DR   PaxDb; 9606-ENSP00000455711; -.
DR   PeptideAtlas; Q9BWW7; -.
DR   ProteomicsDB; 79334; -.
DR   Antibodypedia; 59727; 30 antibodies from 14 providers.
DR   DNASU; 83482; -.
DR   Ensembl; ENST00000569446.3; ENSP00000455711.1; ENSG00000261678.3.
DR   Ensembl; ENST00000645782.2; ENSP00000496266.1; ENSG00000284923.2.
DR   GeneID; 83482; -.
DR   KEGG; hsa:83482; -.
DR   MANE-Select; ENST00000569446.3; ENSP00000455711.1; NM_031309.6; NP_112599.2.
DR   UCSC; uc033ccm.1; human.
DR   AGR; HGNC:15950; -.
DR   CTD; 83482; -.
DR   DisGeNET; 83482; -.
DR   GeneCards; SCRT1; -.
DR   HGNC; HGNC:15950; SCRT1.
DR   HPA; ENSG00000261678; Tissue enriched (brain).
DR   MIM; 605858; gene.
DR   neXtProt; NX_Q9BWW7; -.
DR   OpenTargets; ENSG00000261678; -.
DR   PharmGKB; PA35015; -.
DR   VEuPathDB; HostDB:ENSG00000261678; -.
DR   eggNOG; KOG2462; Eukaryota.
DR   GeneTree; ENSGT00940000154491; -.
DR   HOGENOM; CLU_002678_42_3_1; -.
DR   InParanoid; Q9BWW7; -.
DR   OMA; RCHKAFA; -.
DR   OrthoDB; 658032at2759; -.
DR   PhylomeDB; Q9BWW7; -.
DR   TreeFam; TF315515; -.
DR   PathwayCommons; Q9BWW7; -.
DR   BioGRID-ORCS; 83482; 46 hits in 1164 CRISPR screens.
DR   GenomeRNAi; 83482; -.
DR   Pharos; Q9BWW7; Tbio.
DR   PRO; PR:Q9BWW7; -.
DR   Proteomes; UP000005640; Chromosome 8.
DR   RNAct; Q9BWW7; Protein.
DR   Bgee; ENSG00000261678; Expressed in primary visual cortex and 35 other cell types or tissues.
DR   GO; GO:0016604; C:nuclear body; IDA:HPA.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IBA:GO_Central.
DR   GO; GO:2001222; P:regulation of neuron migration; IEA:Ensembl.
DR   Gene3D; 3.30.160.60; Classic Zinc Finger; 4.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   PANTHER; PTHR24388:SF60; TRANSCRIPTIONAL REPRESSOR SCRATCH 1; 1.
DR   PANTHER; PTHR24388; ZINC FINGER PROTEIN; 1.
DR   Pfam; PF00096; zf-C2H2; 4.
DR   Pfam; PF13912; zf-C2H2_6; 1.
DR   SMART; SM00355; ZnF_C2H2; 5.
DR   SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 3.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
DR   Genevisible; Q9BWW7; HS.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Repressor;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..348
FT                   /note="Transcriptional repressor scratch 1"
FT                   /id="PRO_0000047036"
FT   ZN_FING         191..213
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         222..244
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         248..270
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         276..298
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         304..327
FT                   /note="C2H2-type 5; atypical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          1..20
FT                   /note="SNAG domain"
FT                   /evidence="ECO:0000250"
FT   REGION          130..189
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         133
FT                   /note="A -> S (in dbSNP:rs7013127)"
FT                   /evidence="ECO:0000269|PubMed:11274425"
FT                   /id="VAR_045990"
SQ   SEQUENCE   348 AA;  35554 MW;  D3BB51709C7795DA CRC64;
     MPRSFLVKKV KLDAFSSADL ESAYGRARSD LGAPLHDKGY LSDYVGPSSV YDGDAEAALL
     KGPSPEPMYA AAVRGELGPA AAGSAPPPTP RPELATAAGG YINGDAAVSE GYAADAFFIT
     DGRSRRKASN AGAAAAPSTA SAAAPDGDAG GGGGAGGRSL GSGPGGRGGT RAGAGTEARA
     GPGAAGAGGR HACGECGKTY ATSSNLSRHK QTHRSLDSQL ARRCPTCGKV YVSMPAMAMH
     LLTHDLRHKC GVCGKAFSRP WLLQGHMRSH TGEKPFGCAH CGKAFADRSN LRAHMQTHSA
     FKHFQCKRCK KSFALKSYLN KHYESACFKG GAGGPAAPAP PQLSPVQA
//