Q9BWW4 (SSBP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Single-stranded DNA-binding protein 3

Alternative name(s):
Sequence-specific single-stranded-DNA-binding protein

Gene names

Name:	SSBP3
Synonyms:	SSDP, SSDP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in transcription regulation of the alpha 2(I) collagen gene where it binds to the single-stranded polypyrimidine sequences in the promoter region By similarity.
Subcellular location	Nucleus By similarity.
Tissue specificity	Highly expressed in all hematopoietic tissues, including spleen, lymph node, peripheral blood, bone marrow, thymus, and fetal liver, with highest expression in thymus and fetal liver. Expression is also high in heart, brain, kidney, and skeletal muscle.
Sequence similarities	Contains 1 LisH domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	head morphogenesis Inferred from electronic annotation. Source: Compara midbrain-hindbrain boundary initiation Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of anterior head development Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara prechordal plate formation Inferred from electronic annotation. Source: Compara protein complex assembly Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell protein complex Inferred from electronic annotation. Source: Compara
Molecular_function	single-stranded DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BWW4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BWW4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     123-149: Missing.

Isoform 3 (identifier: Q9BWW4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     150-169: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Single-stranded DNA-binding protein 3		PRO_0000123828
Regions
	Domain	16 – 48	33	LisH
	Compositional bias	98 – 319	222	Pro-rich
	Compositional bias	158 – 369	212	Gly-rich
Amino acid modifications
	Modified residue	347	1	Phosphoserine Ref.7 Ref.9 Ref.10 Ref.11
	Modified residue	352	1	Phosphoserine Ref.9 Ref.11
	Modified residue	360	1	Phosphothreonine Ref.9 Ref.10
	Modified residue	381	1	Phosphoserine Ref.9
	Modified residue	387	1	Phosphoserine Ref.8 Ref.9 Ref.10
Natural variations
	Alternative sequence	123 – 149	27	Missing in isoform 2.		VSP_006260
	Alternative sequence	150 – 169	20	Missing in isoform 3.		VSP_006261

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: DBBB169D4EE10536	FASTA	388	40,421
	10 20 30 40 50 60 MFAKGKGSAV PSDGQAREKL ALYVYEYLLH VGAQKSAQTF LSEIRWEKNI TLGEPPGFLH 70 80 90 100 110 120 SWWCVFWDLY CAAPERRDTC EHSSEAKAFH DYSAAAAPSP VLGNIPPNDG MPGGPIPPGF 130 140 150 160 170 180 FQGPPGSQPS PHAQPPPHNP SSMMGPHSQP FMSPRYAGGP RPPIRMGNQP PGGVPGTQPL 190 200 210 220 230 240 LPNSMDPTRQ QGHPNMGGSM QRMNPPRGMG PMGPGPQNYG SGMRPPPNSL GPAMPGINMG 250 260 270 280 290 300 PGAGRPWPNP NSANSIPYSS SSPGTYVGPP GGGGPPGTPI MPSPADSTNS SDNIYTMINP 310 320 330 340 350 360 VPPGGSRSNF PMGPGSDGPM GGMGGMEPHH MNGSLGSGDI DGLPKNSPNN ISGISNPPGT 370 380 PRDDGELGGN FLHSFQNDNY SPSMTMSV
	Isoform 2 [UniParc]. Checksum: CECDA59F18EFC0B2	FASTA	361	37,697
	10 20 30 40 50 60 MFAKGKGSAV PSDGQAREKL ALYVYEYLLH VGAQKSAQTF LSEIRWEKNI TLGEPPGFLH 70 80 90 100 110 120 SWWCVFWDLY CAAPERRDTC EHSSEAKAFH DYSAAAAPSP VLGNIPPNDG MPGGPIPPGF 130 140 150 160 170 180 FQPFMSPRYA GGPRPPIRMG NQPPGGVPGT QPLLPNSMDP TRQQGHPNMG GSMQRMNPPR 190 200 210 220 230 240 GMGPMGPGPQ NYGSGMRPPP NSLGPAMPGI NMGPGAGRPW PNPNSANSIP YSSSSPGTYV 250 260 270 280 290 300 GPPGGGGPPG TPIMPSPADS TNSSDNIYTM INPVPPGGSR SNFPMGPGSD GPMGGMGGME 310 320 330 340 350 360 PHHMNGSLGS GDIDGLPKNS PNNISGISNP PGTPRDDGEL GGNFLHSFQN DNYSPSMTMS V
	Isoform 3 [UniParc]. Checksum: 87D2E99D9C11B620	FASTA	368	38,210
	10 20 30 40 50 60 MFAKGKGSAV PSDGQAREKL ALYVYEYLLH VGAQKSAQTF LSEIRWEKNI TLGEPPGFLH 70 80 90 100 110 120 SWWCVFWDLY CAAPERRDTC EHSSEAKAFH DYSAAAAPSP VLGNIPPNDG MPGGPIPPGF 130 140 150 160 170 180 FQGPPGSQPS PHAQPPPHNP SSMMGPHSQP PGGVPGTQPL LPNSMDPTRQ QGHPNMGGSM 190 200 210 220 230 240 QRMNPPRGMG PMGPGPQNYG SGMRPPPNSL GPAMPGINMG PGAGRPWPNP NSANSIPYSS 250 260 270 280 290 300 SSPGTYVGPP GGGGPPGTPI MPSPADSTNS SDNIYTMINP VPPGGSRSNF PMGPGSDGPM 310 320 330 340 350 360 GGMGGMEPHH MNGSLGSGDI DGLPKNSPNN ISGISNPPGT PRDDGELGGN FLHSFQNDNY SPSMTMSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel, evolutionarily conserved gene family with putative sequence-specific single-stranded DNA-binding activity." Castro P.D., Liang H., Liang J.C., Nagarajan L. Genomics 80:78-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 2).
[2]	Bayarsaihan D. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Cerebellum.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Eye and Placenta.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-347, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-347; SER-352; THR-360; SER-381 AND SER-387, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-347; THR-360 AND SER-387, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-347 AND SER-352, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY026310 , AY026293, AY026294, AY026295, AY026296, AY026297, AY026298, AY026299, AY026300, AY026301, AY026302, AY026303, AY026304, AY026305, AY026306, AY026307, AY026308, AY026309 Genomic DNA. Translation: AAK21984.1. AY026310 , AY026293, AY026294, AY026295, AY026296, AY026297, AY026299, AY026300, AY026301, AY026302, AY026303, AY026304, AY026305, AY026306, AY026307, AY026308, AY026309 Genomic DNA. Translation: AAK21985.1. AF500116 mRNA. Translation: AAM22101.1. AK289474 mRNA. Translation: BAF82163.1. CH471059 Genomic DNA. Translation: EAX06692.1. AL035415, AL161644 Genomic DNA. Translation: CAI22487.1. AL035415, AL161644 Genomic DNA. Translation: CAI22488.1. AL161644, AL035415 Genomic DNA. Translation: CAI23562.1. AL161644, AL035415 Genomic DNA. Translation: CAI23563.1. BC003605 mRNA. Translation: AAH03605.1. BC066365 mRNA. Translation: AAH66365.1.
IPI	IPI00157304. IPI00215675. IPI00215676.
RefSeq	NP_001009955.1. NM_001009955.2. NP_060540.2. NM_018070.3. NP_663768.1. NM_145716.2.
UniGene	Hs.658676.
3D structure databases
ProteinModelPortal	Q9BWW4.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48897N.
IntAct	Q9BWW4. 2 interactions.
STRING	9606.ENSP00000360371.
PTM databases
PhosphoSite	Q9BWW4.
Polymorphism databases
DMDM	27734581.
Proteomic databases
PaxDb	Q9BWW4.
PRIDE	Q9BWW4.
Protocols and materials databases
DNASU	23648.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000357475; ENSP00000350067; ENSG00000157216. ENST00000371319; ENSP00000360370; ENSG00000157216. ENST00000371320; ENSP00000360371; ENSG00000157216.
GeneID	23648.
KEGG	hsa:23648.
UCSC	uc001cxe.3. human. uc001cxf.3. human. uc001cxg.3. human.
Organism-specific databases
CTD	23648.
GeneCards	GC01M054692.
HGNC	HGNC:15674. SSBP3.
MIM	607390. gene.
neXtProt	NX_Q9BWW4.
PharmGKB	PA38017.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG245801.
HOGENOM	HOG000037785.
HOVERGEN	HBG068487.
InParanoid	Q9BWW4.
OMA	HNPNSMM.
OrthoDB	EOG469QW3.
PhylomeDB	Q9BWW4.
Gene expression databases
ArrayExpress	Q9BWW4.
Bgee	Q9BWW4.
CleanEx	HS_SSBP3.
Genevestigator	Q9BWW4.
GermOnline	ENSG00000157216. Homo sapiens.
Family and domain databases
InterPro	IPR006594. LisH_dimerisation. IPR008116. SSDP_DNA-bd. IPR007591. SSDP_ss-bd. [Graphical view]
PANTHER	PTHR12610. PTHR12610. 1 hit.
Pfam	PF04503. SSDP. 1 hit. [Graphical view]
PRINTS	PR01743. SSDNABINDING.
SMART	SM00667. LisH. 1 hit. [Graphical view]
PROSITE	PS50896. LISH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SSBP3. human.
GenomeRNAi	23648.
NextBio	46471.
SOURCE	Search...

Entry information

Entry name

SSBP3_HUMAN

Accession

Primary (citable) accession number: Q9BWW4
Secondary accession number(s): A8K0A9 , Q5T860, Q5T861, Q9BTM0, Q9BWW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2003
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.