Q9BWU0 (NADAP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kanadaptin

Alternative name(s):
Human lung cancer oncogene 3 protein
Short name=HLC-3
Kidney anion exchanger adapter protein
Solute carrier family 4 anion exchanger member 1 adapter protein

Gene names

Name:	SLC4A1AP
ORF Names:	HLC3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	796 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus. Cytoplasm. Note: Mainly nuclear. Small amounts are found in the cytoplasm. Ref.1
Tissue specificity	Ubiquitously expressed. Ref.1
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Sequence similarities	Contains 1 FHA domain.
Caution	Ref.1 initially suggested a role in targeting SLC4A1 (kidney anion exchanger 1) to the plasma membrane; it does not seem to do so as it does not interact with SLC4A1 and has no effect on SLC4A1 trafficking.
Sequence caution	The sequence AAN12269.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ILK	Q13418	8	EBI-1999704,EBI-747644

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 796	796	Kanadaptin		PRO_0000076268
Regions
	Domain	189 – 249	61	FHA
	Coiled coil	497 – 530	34	Potential
	Compositional bias	644 – 715	72	Glu-rich
Amino acid modifications
	Modified residue	312	1	Phosphoserine Ref.8 Ref.10 Ref.11 Ref.12
	Modified residue	466	1	Phosphoserine Ref.7 Ref.8 Ref.10 Ref.11 Ref.12
	Modified residue	524	1	Phosphoserine Ref.9
	Modified residue	530	1	Phosphoserine Ref.7 Ref.10
	Modified residue	532	1	Phosphoserine Ref.9
	Modified residue	535	1	Phosphoserine Ref.11
	Modified residue	709	1	Phosphoserine Ref.9
	Modified residue	712	1	Phosphoserine Ref.9
	Modified residue	793	1	N6-acetyllysine Ref.13
Natural variations
	Natural variant	139	1	P → T. Ref.2 Ref.5 Corresponds to variant rs9678851 [ dbSNP | Ensembl ].		VAR_024748
	Natural variant	181	1	R → C. Corresponds to variant rs9679004 [ dbSNP | Ensembl ].		VAR_051219
Experimental info
	Sequence conflict	111 – 115	5	SNSGE → PIAKP in AAN12269. Ref.6
	Sequence conflict	309	1	G → R in AAK29177. Ref.2
	Sequence conflict	359	1	Q → R in AAH98358. Ref.5
	Sequence conflict	373	1	A → V in AAK29177. Ref.2
	Sequence conflict	444	1	L → I in AAN12269. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9BWU0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: EE0F560648DA39EF	FASTA	796	88,814
	10 20 30 40 50 60 MLAPLRNAPG REGATSPSPP TDATGSLGEW DVDRNVKTEG WVSKERISKL HRLRMADILS 70 80 90 100 110 120 QSETLASQDL SGDFKKPALP VSPAARSKAP ASSSSNPEEV QKEGPTALQD SNSGEPDIPP 130 140 150 160 170 180 PQPDCGDFRS LQEEQSRPPT AVSSPGGPAR APPYQEPPWG GPATAPYSLE TLKGGTILGT 190 200 210 220 230 240 RSLKGTSYCL FGRLSGCDVC LEHPSVSRYH AVLQHRASGP DGECDSNGPG FYLYDLGSTH 250 260 270 280 290 300 GTFLNKTRIP PRTYCRVHVG HVVRFGGSTR LFILQGPEED REAESELTVT QLKELRKQQQ 310 320 330 340 350 360 ILLEKKMLGE DSDEEEEMDT SERKINAGSQ DDEMGCTWGM GEDAVEDDAE ENPIVLEFQQ 370 380 390 400 410 420 EREAFYIKDP KKALQGFFDR EGEELEYEFD EQGHSTWLCR VRLPVDDSTG KQLVAEAIHS 430 440 450 460 470 480 GKKKEAMIQC SLEACRILDT LGLLRQEAVS RKRKAKNWED EDFYDSDDDT FLDRTGLIEK 490 500 510 520 530 540 KRLNRMKKAG KIDEKPETFE SLVAKLNDAE RELSEISERL KASSQVLSES PSQDSLDAFM 550 560 570 580 590 600 SEMKSGSTLD GVSRKKLHLR TFELRKEQQR LKGLIKIVKP AEIPELKKTE TQTTGAENKA 610 620 630 640 650 660 KKLTLPLFGA MKGGSKFKLK TGTVGKLPPK RPELPPTLMR MKDEPEVEEE EEEEEEEEKE 670 680 690 700 710 720 KEEHEKKKLE DGSLSRPQPE IEPEAAVQEM RPPTDLTHFK ETQTHENMSQ LSEEEQNKDY 730 740 750 760 770 780 QDCSKTTSLC AGPSASKNEY EKSRGELKKK KTPGPGKLPP TLSSKYPEDD PDYCVWVPPE 790 GQSGDGRTHL NDKYGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human kanadaptin and kidney anion exchanger 1 (kAE1) do not interact in transfected HEK 293 cells." Kittanakom S., Keskanokwong T., Akkarapatumwong V., Yenchitsomanus P.-T., Reithmeier R.A.F. Mol. Membr. Biol. 21:395-402(2004) [PubMed: 15764369] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-139.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-139.
[6]	"Identification of a new oncogene in human lung cancer." Kim J.W. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 111-796.
[7]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-466 AND SER-530, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312 AND SER-466, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-524; SER-532; SER-709 AND SER-712, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312; SER-466 AND SER-530, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312; SER-466 AND SER-535, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-312 AND SER-466, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-793, MASS SPECTROMETRY.
[14]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Web resources

Wikipedia

Band 3 entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY028435 mRNA. Translation: AAK29177.1. AK001486 mRNA. Translation: BAA91718.1. AC074091 Genomic DNA. Translation: AAX93203.1. CH471053 Genomic DNA. Translation: EAX00555.1. BC098302 mRNA. Translation: AAH98302.1. BC098358 mRNA. Translation: AAH98358.1. BC099711 mRNA. Translation: AAH99711.1. BC099739 mRNA. Translation: AAH99739.1. AY117688 mRNA. Translation: AAN12269.1. Different initiation.
IPI	IPI00306749.
RefSeq	NP_060628.2. NM_018158.2.
UniGene	Hs.306000.
3D structure databases
ProteinModelPortal	Q9BWU0.
SMR	Q9BWU0. Positions 149-275.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BWU0. 3 interactions.
STRING	Q9BWU0.
PTM databases
PhosphoSite	Q9BWU0.
Polymorphism databases
DMDM	74724887.
Proteomic databases
PRIDE	Q9BWU0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000326019; ENSP00000323837; ENSG00000163798.
GeneID	22950.
KEGG	hsa:22950.
UCSC	uc002rlk.2. human.
Organism-specific databases
CTD	22950.
GeneCards	GC02P027886.
H-InvDB	HIX0001931.
HGNC	HGNC:13813. SLC4A1AP.
HPA	HPA036680.
MIM	602655. gene.
neXtProt	NX_Q9BWU0.
PharmGKB	PA37811.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13433.
GeneTree	ENSGT00510000048003.
HOGENOM	HBG444666.
HOVERGEN	HBG052984.
InParanoid	Q9BWU0.
OMA	PPTLMRM.
OrthoDB	EOG4T4CTV.
PhylomeDB	Q9BWU0.
Gene expression databases
ArrayExpress	Q9BWU0.
Bgee	Q9BWU0.
CleanEx	HS_SLC4A1AP.
Genevestigator	Q9BWU0.
GermOnline	ENSG00000163798. Homo sapiens.
Family and domain databases
InterPro	IPR001159. Ds-RNA-bd. IPR000253. FHA_dom. IPR008984. SMAD_FHA_domain. [Graphical view]
Gene3D	G3DSA:2.60.200.20. FHA. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00498. FHA. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00240. FHA. 1 hit. [Graphical view]
SUPFAM	SSF49879. SMAD_FHA. 1 hit.
PROSITE	PS50006. FHA_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	43719.
SOURCE	Search...

Entry information

Entry name

NADAP_HUMAN

Accession

Primary (citable) accession number: Q9BWU0
Secondary accession number(s): A6NJ39 , Q4KMT1, Q4KMX0, Q7Z5Q9, Q9NVN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.