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Protein

Poly(A) polymerase gamma

Gene

PAPOLG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.2 Publications

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).3 Publications

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Kineticsi

  1. KM=0.051 µM for poly(A)(15)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei108ATPBy similarity1
    Metal bindingi112Magnesium 1; catalytic1
    Metal bindingi112Magnesium 2; catalyticBy similarity1
    Metal bindingi114Magnesium 1; catalytic1
    Metal bindingi114Magnesium 2; catalyticBy similarity1
    Metal bindingi166Magnesium 2; catalyticBy similarity1
    Binding sitei166ATPBy similarity1
    Binding sitei227ATP1 Publication1
    Binding sitei236ATP1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi99 – 101ATP1 Publication3
    Nucleotide bindingi112 – 114ATP1 Publication3
    Nucleotide bindingi245 – 246ATP1 Publication2

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • metal ion binding Source: UniProtKB
    • polynucleotide adenylyltransferase activity Source: UniProtKB
    • RNA binding Source: UniProtKB-KW

    GO - Biological processi

    • mRNA polyadenylation Source: GO_Central
    • RNA polyadenylation Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciZFISH:HS03887-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase gamma (EC:2.7.7.19)
    Short name:
    PAP-gamma
    Alternative name(s):
    Neo-poly(A) polymerase
    Short name:
    Neo-PAP
    Polynucleotide adenylyltransferase gamma
    SRP RNA 3'-adenylating enzyme
    Signal recognition particle RNA-adenylating enzyme
    Short name:
    SRP RNA-adenylating enzyme
    Gene namesi
    Name:PAPOLG
    Synonyms:PAP2, PAPG
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:14982. PAPOLG.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: HPA
    • membrane Source: UniProtKB
    • nucleoplasm Source: HPA
    • nucleus Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi64895.
    OpenTargetsiENSG00000115421.
    PharmGKBiPA32934.

    Polymorphism and mutation databases

    BioMutaiPAPOLG.
    DMDMi116242699.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000516241 – 736Poly(A) polymerase gammaAdd BLAST736

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N6-acetyllysineCombined sources1
    Modified residuei23PhosphoserineCombined sources1
    Modified residuei29PhosphoserineCombined sources1
    Modified residuei525PhosphoserineCombined sources1
    Modified residuei599PhosphoserineCombined sources1
    Modified residuei648PhosphoserineCombined sources1
    Modified residuei654PhosphothreonineCombined sources1
    Modified residuei684PhosphoserineCombined sources1
    Modified residuei708PhosphoserineCombined sources1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    EPDiQ9BWT3.
    MaxQBiQ9BWT3.
    PaxDbiQ9BWT3.
    PeptideAtlasiQ9BWT3.
    PRIDEiQ9BWT3.

    PTM databases

    iPTMnetiQ9BWT3.
    PhosphoSitePlusiQ9BWT3.

    Expressioni

    Tissue specificityi

    Expressed predominantly in testis, and weakly in other tissues. Overexpressed in several tumors.1 Publication

    Gene expression databases

    BgeeiENSG00000115421.
    CleanExiHS_PAPOLG.
    ExpressionAtlasiQ9BWT3. baseline and differential.
    GenevisibleiQ9BWT3. HS.

    Organism-specific databases

    HPAiCAB017795.
    HPA035349.
    HPA035350.

    Interactioni

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sitei152Interaction with RNABy similarity1
    Sitei157Interaction with RNABy similarity1
    Sitei327Interaction with RNABy similarity1
    Sitei398Interaction with RNABy similarity1
    Sitei532Interaction with RNABy similarity1

    Protein-protein interaction databases

    BioGridi122338. 9 interactors.
    IntActiQ9BWT3. 2 interactors.
    STRINGi9606.ENSP00000238714.

    Structurei

    Secondary structure

    1736
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi19 – 22Combined sources4
    Helixi32 – 45Combined sources14
    Helixi46 – 48Combined sources3
    Helixi54 – 78Combined sources25
    Turni79 – 83Combined sources5
    Helixi86 – 89Combined sources4
    Beta strandi95 – 99Combined sources5
    Helixi100 – 104Combined sources5
    Beta strandi113 – 119Combined sources7
    Helixi125 – 138Combined sources14
    Beta strandi142 – 148Combined sources7
    Beta strandi150 – 153Combined sources4
    Beta strandi155 – 160Combined sources6
    Beta strandi163 – 171Combined sources9
    Beta strandi173 – 175Combined sources3
    Helixi186 – 189Combined sources4
    Helixi194 – 211Combined sources18
    Helixi216 – 232Combined sources17
    Turni238 – 241Combined sources4
    Helixi245 – 258Combined sources14
    Helixi264 – 277Combined sources14
    Turni301 – 303Combined sources3
    Helixi307 – 309Combined sources3
    Beta strandi318 – 320Combined sources3
    Helixi330 – 350Combined sources21
    Turni351 – 353Combined sources3
    Helixi357 – 361Combined sources5
    Turni366 – 368Combined sources3
    Beta strandi371 – 382Combined sources12
    Helixi383 – 394Combined sources12
    Helixi397 – 406Combined sources10
    Beta strandi410 – 420Combined sources11
    Beta strandi432 – 442Combined sources11
    Helixi456 – 472Combined sources17
    Beta strandi481 – 489Combined sources9
    Helixi492 – 494Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4LT6X-ray2.79A/B1-508[»]
    ProteinModelPortaliQ9BWT3.
    SMRiQ9BWT3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the poly(A) polymerase family.Curated

    Phylogenomic databases

    eggNOGiKOG2245. Eukaryota.
    COG5186. LUCA.
    GeneTreeiENSGT00390000017928.
    HOGENOMiHOG000204376.
    HOVERGENiHBG053502.
    InParanoidiQ9BWT3.
    KOiK14376.
    OMAiMLDSQRQ.
    OrthoDBiEOG091G0571.
    PhylomeDBiQ9BWT3.
    TreeFamiTF300842.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    IPR002934. Polymerase_NTP_transf_dom.
    [Graphical view]
    PANTHERiPTHR10682. PTHR10682. 1 hit.
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 2 hits.
    [Graphical view]
    SUPFAMiSSF55003. SSF55003. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9BWT3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKEMSANTVL DSQRQQKHYG ITSPISLASP KEIDHIYTQK LIDAMKPFGV
    60 70 80 90 100
    FEDEEELNHR LVVLGKLNNL VKEWISDVSE SKNLPPSVVA TVGGKIFTFG
    110 120 130 140 150
    SYRLGVHTKG ADIDALCVAP RHVERSDFFQ SFFEKLKHQD GIRNLRAVED
    160 170 180 190 200
    AFVPVIKFEF DGIEIDLVFA RLAIQTISDN LDLRDDSRLR SLDIRCIRSL
    210 220 230 240 250
    NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML GFLGGVSWAM
    260 270 280 290 300
    LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD
    310 320 330 340 350
    PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI
    360 370 380 390 400
    LQGKSDWSKL LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL
    410 420 430 440 450
    VGNLERNEFI TLAHVNPQSF PGNKEHHKDN NYVSMWFLGI IFRRVENAES
    460 470 480 490 500
    VNIDLTYDIQ SFTDTVYRQA NNINMLKEGM KIEATHVKKK QLHHYLPAEI
    510 520 530 540 550
    LQKKKKQSLS DVNRSSGGLQ SKRLSLDSSC LDSSRDTDNG TPFNSPASKS
    560 570 580 590 600
    DSPSVGETER NSAEPAAVIV EKPLSVPPAQ GLSIPVIGAK VDSTVKTVSP
    610 620 630 640 650
    PTVCTIPTVV GRNVIPRITT PHNPAQGQPH LNGMSNITKT VTPKRSHSPS
    660 670 680 690 700
    IDGTPKRLKD VEKFIRLEST FKDPRTAEER KRKSVDAIGG ESMPIPTIDT
    710 720 730
    SRKKRLPSKE LPDSSSPVPA NNIRVIKNSI RLTLNR
    Length:736
    Mass (Da):82,803
    Last modified:October 17, 2006 - v2
    Checksum:iB0F6F217AA36E552
    GO
    Isoform 2 (identifier: Q9BWT3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         664-685: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:714
    Mass (Da):80,127
    Checksum:i0467FCF53C3CE806
    GO

    Sequence cautioni

    The sequence BAB14604 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti31K → E in AAK31791 (PubMed:11287430).Curated1
    Sequence conflicti289P → S in AAK31791 (PubMed:11287430).Curated1
    Sequence conflicti619T → I in AAK31791 (PubMed:11287430).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_054379664 – 685Missing in isoform 2. 1 PublicationAdd BLAST22

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY029162 mRNA. Translation: AAK31791.1.
    AF312211 mRNA. Translation: AAK83701.1.
    AJ308101 mRNA. Translation: CAC59751.1.
    AK021867 mRNA. Translation: BAB13919.1.
    AK023544 mRNA. Translation: BAB14604.1. Different initiation.
    AK314663 mRNA. Translation: BAG37221.1.
    AC011245 Genomic DNA. No translation available.
    AC012498 Genomic DNA. No translation available.
    CH471053 Genomic DNA. Translation: EAX00034.1.
    BC111701 mRNA. Translation: AAI11702.1.
    BC113747 mRNA. Translation: AAI13748.1.
    AB209304 mRNA. Translation: BAD92541.1.
    CCDSiCCDS1863.1. [Q9BWT3-1]
    RefSeqiNP_075045.2. NM_022894.3. [Q9BWT3-1]
    XP_005264557.1. XM_005264500.3. [Q9BWT3-2]
    UniGeneiHs.387471.

    Genome annotation databases

    EnsembliENST00000238714; ENSP00000238714; ENSG00000115421. [Q9BWT3-1]
    GeneIDi64895.
    KEGGihsa:64895.
    UCSCiuc002sai.4. human. [Q9BWT3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY029162 mRNA. Translation: AAK31791.1.
    AF312211 mRNA. Translation: AAK83701.1.
    AJ308101 mRNA. Translation: CAC59751.1.
    AK021867 mRNA. Translation: BAB13919.1.
    AK023544 mRNA. Translation: BAB14604.1. Different initiation.
    AK314663 mRNA. Translation: BAG37221.1.
    AC011245 Genomic DNA. No translation available.
    AC012498 Genomic DNA. No translation available.
    CH471053 Genomic DNA. Translation: EAX00034.1.
    BC111701 mRNA. Translation: AAI11702.1.
    BC113747 mRNA. Translation: AAI13748.1.
    AB209304 mRNA. Translation: BAD92541.1.
    CCDSiCCDS1863.1. [Q9BWT3-1]
    RefSeqiNP_075045.2. NM_022894.3. [Q9BWT3-1]
    XP_005264557.1. XM_005264500.3. [Q9BWT3-2]
    UniGeneiHs.387471.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4LT6X-ray2.79A/B1-508[»]
    ProteinModelPortaliQ9BWT3.
    SMRiQ9BWT3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi122338. 9 interactors.
    IntActiQ9BWT3. 2 interactors.
    STRINGi9606.ENSP00000238714.

    PTM databases

    iPTMnetiQ9BWT3.
    PhosphoSitePlusiQ9BWT3.

    Polymorphism and mutation databases

    BioMutaiPAPOLG.
    DMDMi116242699.

    Proteomic databases

    EPDiQ9BWT3.
    MaxQBiQ9BWT3.
    PaxDbiQ9BWT3.
    PeptideAtlasiQ9BWT3.
    PRIDEiQ9BWT3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000238714; ENSP00000238714; ENSG00000115421. [Q9BWT3-1]
    GeneIDi64895.
    KEGGihsa:64895.
    UCSCiuc002sai.4. human. [Q9BWT3-1]

    Organism-specific databases

    CTDi64895.
    DisGeNETi64895.
    GeneCardsiPAPOLG.
    HGNCiHGNC:14982. PAPOLG.
    HPAiCAB017795.
    HPA035349.
    HPA035350.
    MIMi616865. gene.
    neXtProtiNX_Q9BWT3.
    OpenTargetsiENSG00000115421.
    PharmGKBiPA32934.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG2245. Eukaryota.
    COG5186. LUCA.
    GeneTreeiENSGT00390000017928.
    HOGENOMiHOG000204376.
    HOVERGENiHBG053502.
    InParanoidiQ9BWT3.
    KOiK14376.
    OMAiMLDSQRQ.
    OrthoDBiEOG091G0571.
    PhylomeDBiQ9BWT3.
    TreeFamiTF300842.

    Enzyme and pathway databases

    BioCyciZFISH:HS03887-MONOMER.

    Miscellaneous databases

    GeneWikiiPAPOLG.
    GenomeRNAii64895.
    PROiQ9BWT3.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000115421.
    CleanExiHS_PAPOLG.
    ExpressionAtlasiQ9BWT3. baseline and differential.
    GenevisibleiQ9BWT3. HS.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    IPR002934. Polymerase_NTP_transf_dom.
    [Graphical view]
    PANTHERiPTHR10682. PTHR10682. 1 hit.
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 2 hits.
    [Graphical view]
    SUPFAMiSSF55003. SSF55003. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPAPOG_HUMAN
    AccessioniPrimary (citable) accession number: Q9BWT3
    Secondary accession number(s): B2RBH4
    , Q59G05, Q969N1, Q9H8L2, Q9HAD0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: October 17, 2006
    Last modified: November 2, 2016
    This is version 145 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.