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Q9BWT3 (PAPOG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) polymerase gamma

Short name=PAP-gamma
EC=2.7.7.19
Alternative name(s):
Neo-poly(A) polymerase
Short name=Neo-PAP
Polynucleotide adenylyltransferase gamma
SRP RNA 3'-adenylating enzyme
Gene names
Name:PAPOLG
Synonyms:PAP2, PAPG
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA. Ref.1 Ref.2

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1). Ref.1 Ref.2

Cofactor

Binds 2 magnesium ions. Also active with manganese By similarity.

Subcellular location

Nucleus Ref.1 Ref.2.

Tissue specificity

Expressed predominantly in testis, and weakly in other tissues. Overexpressed in several tumors. Ref.2

Sequence similarities

Belongs to the poly(A) polymerase family.

Sequence caution

The sequence BAB14604.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BWT3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BWT3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     664-685: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 736736Poly(A) polymerase gamma
PRO_0000051624

Regions

Nucleotide binding99 – 1013ATP
Nucleotide binding112 – 1143ATP
Nucleotide binding245 – 2462ATP

Sites

Metal binding1121Magnesium 1; catalytic
Metal binding1121Magnesium 2; catalytic By similarity
Metal binding1141Magnesium 1; catalytic
Metal binding1141Magnesium 2; catalytic By similarity
Metal binding1661Magnesium 2; catalytic By similarity
Binding site1081ATP By similarity
Binding site1661ATP By similarity
Binding site2271ATP
Binding site2361ATP
Site1521Interaction with RNA By similarity
Site1571Interaction with RNA By similarity
Site3271Interaction with RNA By similarity
Site3981Interaction with RNA By similarity
Site5321Interaction with RNA By similarity

Amino acid modifications

Modified residue21N6-acetyllysine Ref.14
Modified residue231Phosphoserine Ref.10
Modified residue6481Phosphoserine Ref.10
Modified residue6541Phosphothreonine Ref.10
Modified residue6841Phosphoserine Ref.10 Ref.12

Natural variations

Alternative sequence664 – 68522Missing in isoform 2.
VSP_054379

Experimental info

Sequence conflict311K → E in AAK31791. Ref.1
Sequence conflict2891P → S in AAK31791. Ref.1
Sequence conflict6191T → I in AAK31791. Ref.1

Secondary structure

.................................................................... 736
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: B0F6F217AA36E552

FASTA73682,803
        10         20         30         40         50         60 
MKEMSANTVL DSQRQQKHYG ITSPISLASP KEIDHIYTQK LIDAMKPFGV FEDEEELNHR 

        70         80         90        100        110        120 
LVVLGKLNNL VKEWISDVSE SKNLPPSVVA TVGGKIFTFG SYRLGVHTKG ADIDALCVAP 

       130        140        150        160        170        180 
RHVERSDFFQ SFFEKLKHQD GIRNLRAVED AFVPVIKFEF DGIEIDLVFA RLAIQTISDN 

       190        200        210        220        230        240 
LDLRDDSRLR SLDIRCIRSL NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML 

       250        260        270        280        290        300 
GFLGGVSWAM LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD 

       310        320        330        340        350        360 
PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI LQGKSDWSKL 

       370        380        390        400        410        420 
LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL VGNLERNEFI TLAHVNPQSF 

       430        440        450        460        470        480 
PGNKEHHKDN NYVSMWFLGI IFRRVENAES VNIDLTYDIQ SFTDTVYRQA NNINMLKEGM 

       490        500        510        520        530        540 
KIEATHVKKK QLHHYLPAEI LQKKKKQSLS DVNRSSGGLQ SKRLSLDSSC LDSSRDTDNG 

       550        560        570        580        590        600 
TPFNSPASKS DSPSVGETER NSAEPAAVIV EKPLSVPPAQ GLSIPVIGAK VDSTVKTVSP 

       610        620        630        640        650        660 
PTVCTIPTVV GRNVIPRITT PHNPAQGQPH LNGMSNITKT VTPKRSHSPS IDGTPKRLKD 

       670        680        690        700        710        720 
VEKFIRLEST FKDPRTAEER KRKSVDAIGG ESMPIPTIDT SRKKRLPSKE LPDSSSPVPA 

       730 
NNIRVIKNSI RLTLNR 

« Hide

Isoform 2 [UniParc].

Checksum: 0467FCF53C3CE806
Show »

FASTA71480,127

References

« Hide 'large scale' references
[1]"Purification, characterization, and cloning of the cDNA of human signal recognition particle RNA 3'-adenylating enzyme."
Perumal K., Sinha K., Henning D., Reddy R.
J. Biol. Chem. 276:21791-21796(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
[2]"Identification and functional characterization of neo-poly(A) polymerase, an RNA processing enzyme overexpressed in human tumors."
Topalian S.L., Kaneko S., Gonzales M.I., Bond G.L., Ward Y., Manley J.L.
Mol. Cell. Biol. 21:5614-5623(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Tissue: Melanoma.
[3]"A novel nuclear human poly(A) polymerase, PAPg."
Kyriakopoulou C.B., Virtanen A.
Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Cervix carcinoma.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Embryo and Placenta.
[5]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[8]"Homo sapiens protein coding cDNA."
Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 333-736 (ISOFORM 2).
Tissue: Brain.
[9]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-648; THR-654 AND SER-684, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-684, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Crystal structure of human poly(A) polymerase gamma reveals a conserved catalytic core for canonical poly(A) polymerases."
Yang Q., Nausch L., Martin G., Keller W., Doublie S.
J. Mol. Biol. 426:43-50(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.79 ANGSTROMS) OF 1-508 IN COMPLEX WITH ATP AND CALCIUM IONS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY029162 mRNA. Translation: AAK31791.1.
AF312211 mRNA. Translation: AAK83701.1.
AJ308101 mRNA. Translation: CAC59751.1.
AK021867 mRNA. Translation: BAB13919.1.
AK023544 mRNA. Translation: BAB14604.1. Different initiation.
AK314663 mRNA. Translation: BAG37221.1.
AC011245 Genomic DNA. No translation available.
AC012498 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00034.1.
BC111701 mRNA. Translation: AAI11702.1.
BC113747 mRNA. Translation: AAI13748.1.
AB209304 mRNA. Translation: BAD92541.1.
CCDSCCDS1863.1.
RefSeqNP_075045.2. NM_022894.3. [Q9BWT3-1]
XP_005264557.1. XM_005264500.1. [Q9BWT3-2]
UniGeneHs.387471.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4LT6X-ray2.79A/B1-508[»]
ProteinModelPortalQ9BWT3.
SMRQ9BWT3. Positions 17-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122338. 5 interactions.
STRING9606.ENSP00000238714.

PTM databases

PhosphoSiteQ9BWT3.

Polymorphism databases

DMDM116242699.

Proteomic databases

MaxQBQ9BWT3.
PaxDbQ9BWT3.
PRIDEQ9BWT3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000238714; ENSP00000238714; ENSG00000115421.
GeneID64895.
KEGGhsa:64895.
UCSCuc002sai.3. human. [Q9BWT3-1]

Organism-specific databases

CTD64895.
GeneCardsGC02P060983.
HGNCHGNC:14982. PAPOLG.
HPACAB017795.
HPA035349.
HPA035350.
neXtProtNX_Q9BWT3.
PharmGKBPA32934.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5186.
HOGENOMHOG000204376.
HOVERGENHBG053502.
InParanoidQ9BWT3.
KOK14376.
OMAGECMPIP.
OrthoDBEOG7XH6PT.
PhylomeDBQ9BWT3.
TreeFamTF300842.

Gene expression databases

ArrayExpressQ9BWT3.
BgeeQ9BWT3.
CleanExHS_PAPOLG.
GenevestigatorQ9BWT3.

Family and domain databases

Gene3D3.30.70.590. 1 hit.
InterProIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMSSF55003. SSF55003. 1 hit.
ProtoNetSearch...

Other

GeneWikiPAPOLG.
GenomeRNAi64895.
NextBio67037.
PROQ9BWT3.

Entry information

Entry namePAPOG_HUMAN
AccessionPrimary (citable) accession number: Q9BWT3
Secondary accession number(s): B2RBH4 expand/collapse secondary AC list , Q59G05, Q969N1, Q9H8L2, Q9HAD0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 17, 2006
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM