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Protein

Poly(A) polymerase gamma

Gene

PAPOLG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.2 Publications

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).3 Publications

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Kineticsi

  1. KM=0.051 µM for poly(A)(15)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei108 – 1081ATPBy similarity
    Metal bindingi112 – 1121Magnesium 1; catalytic
    Metal bindingi112 – 1121Magnesium 2; catalyticBy similarity
    Metal bindingi114 – 1141Magnesium 1; catalytic
    Metal bindingi114 – 1141Magnesium 2; catalyticBy similarity
    Metal bindingi166 – 1661Magnesium 2; catalyticBy similarity
    Binding sitei166 – 1661ATPBy similarity
    Binding sitei227 – 2271ATP1 Publication
    Binding sitei236 – 2361ATP1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi99 – 1013ATP1 Publication
    Nucleotide bindingi112 – 1143ATP1 Publication
    Nucleotide bindingi245 – 2462ATP1 Publication

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • metal ion binding Source: UniProtKB
    • polynucleotide adenylyltransferase activity Source: UniProtKB
    • RNA binding Source: UniProtKB-KW

    GO - Biological processi

    • mRNA polyadenylation Source: GO_Central
    • RNA polyadenylation Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase gamma (EC:2.7.7.19)
    Short name:
    PAP-gamma
    Alternative name(s):
    Neo-poly(A) polymerase
    Short name:
    Neo-PAP
    Polynucleotide adenylyltransferase gamma
    SRP RNA 3'-adenylating enzyme
    Signal recognition particle RNA-adenylating enzyme
    Short name:
    SRP RNA-adenylating enzyme
    Gene namesi
    Name:PAPOLG
    Synonyms:PAP2, PAPG
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:14982. PAPOLG.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: HPA
    • membrane Source: UniProtKB
    • nucleoplasm Source: HPA
    • nucleus Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA32934.

    Polymorphism and mutation databases

    BioMutaiPAPOLG.
    DMDMi116242699.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 736736Poly(A) polymerase gammaPRO_0000051624Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N6-acetyllysineCombined sources
    Modified residuei23 – 231PhosphoserineCombined sources
    Modified residuei29 – 291PhosphoserineCombined sources
    Modified residuei525 – 5251PhosphoserineCombined sources
    Modified residuei545 – 5451PhosphoserineBy similarity
    Modified residuei562 – 5621PhosphoserineBy similarity
    Modified residuei599 – 5991PhosphoserineCombined sources
    Modified residuei639 – 6391N6-acetyllysineBy similarity
    Modified residuei644 – 6441N6-acetyllysineBy similarity
    Modified residuei648 – 6481PhosphoserineCombined sources
    Modified residuei654 – 6541PhosphothreonineCombined sources
    Modified residuei684 – 6841PhosphoserineCombined sources
    Modified residuei708 – 7081PhosphoserineCombined sources
    Modified residuei727 – 7271N6-acetyllysineBy similarity
    Modified residuei729 – 7291PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    EPDiQ9BWT3.
    MaxQBiQ9BWT3.
    PaxDbiQ9BWT3.
    PeptideAtlasiQ9BWT3.
    PRIDEiQ9BWT3.

    PTM databases

    iPTMnetiQ9BWT3.
    PhosphoSiteiQ9BWT3.

    Expressioni

    Tissue specificityi

    Expressed predominantly in testis, and weakly in other tissues. Overexpressed in several tumors.1 Publication

    Gene expression databases

    BgeeiENSG00000115421.
    CleanExiHS_PAPOLG.
    ExpressionAtlasiQ9BWT3. baseline and differential.
    GenevisibleiQ9BWT3. HS.

    Organism-specific databases

    HPAiCAB017795.
    HPA035349.
    HPA035350.

    Interactioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei152 – 1521Interaction with RNABy similarity
    Sitei157 – 1571Interaction with RNABy similarity
    Sitei327 – 3271Interaction with RNABy similarity
    Sitei398 – 3981Interaction with RNABy similarity
    Sitei532 – 5321Interaction with RNABy similarity

    Protein-protein interaction databases

    BioGridi122338. 9 interactions.
    IntActiQ9BWT3. 2 interactions.
    STRINGi9606.ENSP00000238714.

    Structurei

    Secondary structure

    1
    736
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi19 – 224Combined sources
    Helixi32 – 4514Combined sources
    Helixi46 – 483Combined sources
    Helixi54 – 7825Combined sources
    Turni79 – 835Combined sources
    Helixi86 – 894Combined sources
    Beta strandi95 – 995Combined sources
    Helixi100 – 1045Combined sources
    Beta strandi113 – 1197Combined sources
    Helixi125 – 13814Combined sources
    Beta strandi142 – 1487Combined sources
    Beta strandi150 – 1534Combined sources
    Beta strandi155 – 1606Combined sources
    Beta strandi163 – 1719Combined sources
    Beta strandi173 – 1753Combined sources
    Helixi186 – 1894Combined sources
    Helixi194 – 21118Combined sources
    Helixi216 – 23217Combined sources
    Turni238 – 2414Combined sources
    Helixi245 – 25814Combined sources
    Helixi264 – 27714Combined sources
    Turni301 – 3033Combined sources
    Helixi307 – 3093Combined sources
    Beta strandi318 – 3203Combined sources
    Helixi330 – 35021Combined sources
    Turni351 – 3533Combined sources
    Helixi357 – 3615Combined sources
    Turni366 – 3683Combined sources
    Beta strandi371 – 38212Combined sources
    Helixi383 – 39412Combined sources
    Helixi397 – 40610Combined sources
    Beta strandi410 – 42011Combined sources
    Beta strandi432 – 44211Combined sources
    Helixi456 – 47217Combined sources
    Beta strandi481 – 4899Combined sources
    Helixi492 – 4943Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4LT6X-ray2.79A/B1-508[»]
    ProteinModelPortaliQ9BWT3.
    SMRiQ9BWT3. Positions 17-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the poly(A) polymerase family.Curated

    Phylogenomic databases

    eggNOGiKOG2245. Eukaryota.
    COG5186. LUCA.
    GeneTreeiENSGT00390000017928.
    HOGENOMiHOG000204376.
    HOVERGENiHBG053502.
    InParanoidiQ9BWT3.
    KOiK14376.
    OMAiMLDSQRQ.
    OrthoDBiEOG091G0571.
    PhylomeDBiQ9BWT3.
    TreeFamiTF300842.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    IPR002934. Polymerase_NTP_transf_dom.
    [Graphical view]
    PANTHERiPTHR10682. PTHR10682. 1 hit.
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 2 hits.
    [Graphical view]
    SUPFAMiSSF55003. SSF55003. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q9BWT3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKEMSANTVL DSQRQQKHYG ITSPISLASP KEIDHIYTQK LIDAMKPFGV
    60 70 80 90 100
    FEDEEELNHR LVVLGKLNNL VKEWISDVSE SKNLPPSVVA TVGGKIFTFG
    110 120 130 140 150
    SYRLGVHTKG ADIDALCVAP RHVERSDFFQ SFFEKLKHQD GIRNLRAVED
    160 170 180 190 200
    AFVPVIKFEF DGIEIDLVFA RLAIQTISDN LDLRDDSRLR SLDIRCIRSL
    210 220 230 240 250
    NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML GFLGGVSWAM
    260 270 280 290 300
    LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD
    310 320 330 340 350
    PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI
    360 370 380 390 400
    LQGKSDWSKL LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL
    410 420 430 440 450
    VGNLERNEFI TLAHVNPQSF PGNKEHHKDN NYVSMWFLGI IFRRVENAES
    460 470 480 490 500
    VNIDLTYDIQ SFTDTVYRQA NNINMLKEGM KIEATHVKKK QLHHYLPAEI
    510 520 530 540 550
    LQKKKKQSLS DVNRSSGGLQ SKRLSLDSSC LDSSRDTDNG TPFNSPASKS
    560 570 580 590 600
    DSPSVGETER NSAEPAAVIV EKPLSVPPAQ GLSIPVIGAK VDSTVKTVSP
    610 620 630 640 650
    PTVCTIPTVV GRNVIPRITT PHNPAQGQPH LNGMSNITKT VTPKRSHSPS
    660 670 680 690 700
    IDGTPKRLKD VEKFIRLEST FKDPRTAEER KRKSVDAIGG ESMPIPTIDT
    710 720 730
    SRKKRLPSKE LPDSSSPVPA NNIRVIKNSI RLTLNR
    Length:736
    Mass (Da):82,803
    Last modified:October 17, 2006 - v2
    Checksum:iB0F6F217AA36E552
    GO
    Isoform 2 (identifier: Q9BWT3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         664-685: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:714
    Mass (Da):80,127
    Checksum:i0467FCF53C3CE806
    GO

    Sequence cautioni

    The sequence BAB14604 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti31 – 311K → E in AAK31791 (PubMed:11287430).Curated
    Sequence conflicti289 – 2891P → S in AAK31791 (PubMed:11287430).Curated
    Sequence conflicti619 – 6191T → I in AAK31791 (PubMed:11287430).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei664 – 68522Missing in isoform 2. 1 PublicationVSP_054379Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY029162 mRNA. Translation: AAK31791.1.
    AF312211 mRNA. Translation: AAK83701.1.
    AJ308101 mRNA. Translation: CAC59751.1.
    AK021867 mRNA. Translation: BAB13919.1.
    AK023544 mRNA. Translation: BAB14604.1. Different initiation.
    AK314663 mRNA. Translation: BAG37221.1.
    AC011245 Genomic DNA. No translation available.
    AC012498 Genomic DNA. No translation available.
    CH471053 Genomic DNA. Translation: EAX00034.1.
    BC111701 mRNA. Translation: AAI11702.1.
    BC113747 mRNA. Translation: AAI13748.1.
    AB209304 mRNA. Translation: BAD92541.1.
    CCDSiCCDS1863.1. [Q9BWT3-1]
    RefSeqiNP_075045.2. NM_022894.3. [Q9BWT3-1]
    XP_005264557.1. XM_005264500.3. [Q9BWT3-2]
    UniGeneiHs.387471.

    Genome annotation databases

    EnsembliENST00000238714; ENSP00000238714; ENSG00000115421. [Q9BWT3-1]
    GeneIDi64895.
    KEGGihsa:64895.
    UCSCiuc002sai.4. human. [Q9BWT3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY029162 mRNA. Translation: AAK31791.1.
    AF312211 mRNA. Translation: AAK83701.1.
    AJ308101 mRNA. Translation: CAC59751.1.
    AK021867 mRNA. Translation: BAB13919.1.
    AK023544 mRNA. Translation: BAB14604.1. Different initiation.
    AK314663 mRNA. Translation: BAG37221.1.
    AC011245 Genomic DNA. No translation available.
    AC012498 Genomic DNA. No translation available.
    CH471053 Genomic DNA. Translation: EAX00034.1.
    BC111701 mRNA. Translation: AAI11702.1.
    BC113747 mRNA. Translation: AAI13748.1.
    AB209304 mRNA. Translation: BAD92541.1.
    CCDSiCCDS1863.1. [Q9BWT3-1]
    RefSeqiNP_075045.2. NM_022894.3. [Q9BWT3-1]
    XP_005264557.1. XM_005264500.3. [Q9BWT3-2]
    UniGeneiHs.387471.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4LT6X-ray2.79A/B1-508[»]
    ProteinModelPortaliQ9BWT3.
    SMRiQ9BWT3. Positions 17-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi122338. 9 interactions.
    IntActiQ9BWT3. 2 interactions.
    STRINGi9606.ENSP00000238714.

    PTM databases

    iPTMnetiQ9BWT3.
    PhosphoSiteiQ9BWT3.

    Polymorphism and mutation databases

    BioMutaiPAPOLG.
    DMDMi116242699.

    Proteomic databases

    EPDiQ9BWT3.
    MaxQBiQ9BWT3.
    PaxDbiQ9BWT3.
    PeptideAtlasiQ9BWT3.
    PRIDEiQ9BWT3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000238714; ENSP00000238714; ENSG00000115421. [Q9BWT3-1]
    GeneIDi64895.
    KEGGihsa:64895.
    UCSCiuc002sai.4. human. [Q9BWT3-1]

    Organism-specific databases

    CTDi64895.
    GeneCardsiPAPOLG.
    HGNCiHGNC:14982. PAPOLG.
    HPAiCAB017795.
    HPA035349.
    HPA035350.
    MIMi616865. gene.
    neXtProtiNX_Q9BWT3.
    PharmGKBiPA32934.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG2245. Eukaryota.
    COG5186. LUCA.
    GeneTreeiENSGT00390000017928.
    HOGENOMiHOG000204376.
    HOVERGENiHBG053502.
    InParanoidiQ9BWT3.
    KOiK14376.
    OMAiMLDSQRQ.
    OrthoDBiEOG091G0571.
    PhylomeDBiQ9BWT3.
    TreeFamiTF300842.

    Miscellaneous databases

    GeneWikiiPAPOLG.
    GenomeRNAii64895.
    PROiQ9BWT3.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000115421.
    CleanExiHS_PAPOLG.
    ExpressionAtlasiQ9BWT3. baseline and differential.
    GenevisibleiQ9BWT3. HS.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    IPR002934. Polymerase_NTP_transf_dom.
    [Graphical view]
    PANTHERiPTHR10682. PTHR10682. 1 hit.
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 2 hits.
    [Graphical view]
    SUPFAMiSSF55003. SSF55003. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPAPOG_HUMAN
    AccessioniPrimary (citable) accession number: Q9BWT3
    Secondary accession number(s): B2RBH4
    , Q59G05, Q969N1, Q9H8L2, Q9HAD0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: October 17, 2006
    Last modified: September 7, 2016
    This is version 143 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.