Q9BWT3 (PAPOG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase gamma
Short name=PAP-gamma
EC=2.7.7.19

Alternative name(s):
Neo-poly(A) polymerase
Short name=Neo-PAP
Polynucleotide adenylyltransferase gamma
SRP RNA 3'-adenylating enzyme

Gene names

Name:	PAPOLG
Synonyms:	PAP2, PAPG

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Binds 2 magnesium ions. Also active with manganese By similarity.
Subcellular location	Nucleus Ref.2.
Tissue specificity	Expressed predominantly in testis, and weakly in other tissues. Overexpressed in several tumors. Ref.2
Sequence similarities	Belongs to the poly(A) polymerase family.
Sequence caution	The sequence BAB14604.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding RNA-binding
Molecular function	Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mRNA polyadenylation Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	nucleus Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	Poly(A) polymerase gamma		PRO_0000051624
Regions
	Nucleotide binding	99 – 101	3	ATP By similarity
	Nucleotide binding	112 – 114	3	ATP By similarity
	Nucleotide binding	245 – 246	2	ATP By similarity
Sites
	Metal binding	112	1	Magnesium 1; catalytic By similarity
	Metal binding	112	1	Magnesium 2; catalytic By similarity
	Metal binding	114	1	Magnesium 1; catalytic By similarity
	Metal binding	114	1	Magnesium 2; catalytic By similarity
	Metal binding	166	1	Magnesium 2; catalytic By similarity
	Binding site	108	1	ATP By similarity
	Binding site	166	1	ATP By similarity
	Binding site	227	1	ATP By similarity
	Binding site	236	1	ATP By similarity
	Site	152	1	Interaction with RNA By similarity
	Site	157	1	Interaction with RNA By similarity
	Site	327	1	Interaction with RNA By similarity
	Site	398	1	Interaction with RNA By similarity
	Site	532	1	Interaction with RNA By similarity
Amino acid modifications
	Modified residue	2	1	N6-acetyllysine Ref.11
	Modified residue	23	1	Phosphoserine Ref.8
	Modified residue	552	1	Phosphoserine By similarity
	Modified residue	648	1	Phosphoserine Ref.8
	Modified residue	654	1	Phosphothreonine Ref.8
	Modified residue	684	1	Phosphoserine Ref.8 Ref.9
Experimental info
	Sequence conflict	31	1	K → E in AAK31791. Ref.1
	Sequence conflict	289	1	P → S in AAK31791. Ref.1
	Sequence conflict	619	1	T → I in AAK31791. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9BWT3 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: B0F6F217AA36E552	FASTA	736	82,803
	10 20 30 40 50 60 MKEMSANTVL DSQRQQKHYG ITSPISLASP KEIDHIYTQK LIDAMKPFGV FEDEEELNHR 70 80 90 100 110 120 LVVLGKLNNL VKEWISDVSE SKNLPPSVVA TVGGKIFTFG SYRLGVHTKG ADIDALCVAP 130 140 150 160 170 180 RHVERSDFFQ SFFEKLKHQD GIRNLRAVED AFVPVIKFEF DGIEIDLVFA RLAIQTISDN 190 200 210 220 230 240 LDLRDDSRLR SLDIRCIRSL NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML 250 260 270 280 290 300 GFLGGVSWAM LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD 310 320 330 340 350 360 PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI LQGKSDWSKL 370 380 390 400 410 420 LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL VGNLERNEFI TLAHVNPQSF 430 440 450 460 470 480 PGNKEHHKDN NYVSMWFLGI IFRRVENAES VNIDLTYDIQ SFTDTVYRQA NNINMLKEGM 490 500 510 520 530 540 KIEATHVKKK QLHHYLPAEI LQKKKKQSLS DVNRSSGGLQ SKRLSLDSSC LDSSRDTDNG 550 560 570 580 590 600 TPFNSPASKS DSPSVGETER NSAEPAAVIV EKPLSVPPAQ GLSIPVIGAK VDSTVKTVSP 610 620 630 640 650 660 PTVCTIPTVV GRNVIPRITT PHNPAQGQPH LNGMSNITKT VTPKRSHSPS IDGTPKRLKD 670 680 690 700 710 720 VEKFIRLEST FKDPRTAEER KRKSVDAIGG ESMPIPTIDT SRKKRLPSKE LPDSSSPVPA 730 NNIRVIKNSI RLTLNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, characterization, and cloning of the cDNA of human signal recognition particle RNA 3'-adenylating enzyme." Perumal K., Sinha K., Henning D., Reddy R. J. Biol. Chem. 276:21791-21796(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[2]	"Identification and functional characterization of neo-poly(A) polymerase, an RNA processing enzyme overexpressed in human tumors." Topalian S.L., Kaneko S., Gonzales M.I., Bond G.L., Ward Y., Manley J.L. Mol. Cell. Biol. 21:5614-5623(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Melanoma.
[3]	"A novel nuclear human poly(A) polymerase, PAPg." Kyriakopoulou C.B., Virtanen A. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cervix carcinoma.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo and Placenta.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-23; SER-648; THR-654 AND SER-684, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-684, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-2, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY029162 mRNA. Translation: AAK31791.1. AF312211 mRNA. Translation: AAK83701.1. AJ308101 mRNA. Translation: CAC59751.1. AK021867 mRNA. Translation: BAB13919.1. AK023544 mRNA. Translation: BAB14604.1. Different initiation. AK314663 mRNA. Translation: BAG37221.1. CH471053 Genomic DNA. Translation: EAX00034.1. BC111701 mRNA. Translation: AAI11702.1. BC113747 mRNA. Translation: AAI13748.1.
IPI	IPI00304442.
RefSeq	NP_075045.2. NM_022894.3.
UniGene	Hs.387471.
3D structure databases
ProteinModelPortal	Q9BWT3.
ModBase	Search...
PTM databases
PhosphoSite	Q9BWT3.
Polymorphism databases
DMDM	116242699.
Proteomic databases
PaxDb	Q9BWT3.
PRIDE	Q9BWT3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000238714; ENSP00000238714; ENSG00000115421.
GeneID	64895.
KEGG	hsa:64895.
UCSC	uc002sai.3. human.
Organism-specific databases
CTD	64895.
GeneCards	GC02P060983.
HGNC	HGNC:14982. PAPOLG.
HPA	CAB017795.
neXtProt	NX_Q9BWT3.
PharmGKB	PA32934.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5186.
HOGENOM	HOG000204376.
HOVERGEN	HBG053502.
InParanoid	Q9BWT3.
KO	K14376.
OMA	IDGSSKR.
OrthoDB	EOG4R23T8.
PhylomeDB	Q9BWT3.
Gene expression databases
ArrayExpress	Q9BWT3.
Bgee	Q9BWT3.
CleanEx	HS_PAPOLG.
Genevestigator	Q9BWT3.
GermOnline	ENSG00000115421. Homo sapiens.
Family and domain databases
Gene3D	3.30.70.590. 1 hit.
InterPro	IPR002934. Nucleotidyltransferase. IPR011068. NuclTrfase_I_C. IPR007012. PolA_pol_cen_dom. IPR007010. PolA_pol_RNA-bd_dom. IPR014492. PolyA_polymerase. [Graphical view]
Pfam	PF01909. NTP_transf_2. 1 hit. PF04928. PAP_central. 1 hit. PF04926. PAP_RNA-bind. 1 hit. [Graphical view]
PIRSF	PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAM	SSF55003. PAP_C. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	64895.
NextBio	67037.

Entry information

Entry name

PAPOG_HUMAN

Accession

Primary (citable) accession number: Q9BWT3
Secondary accession number(s): B2RBH4 , Q969N1, Q9H8L2, Q9HAD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.