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Q9BWQ8 (LFG2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein lifeguard 2
Alternative name(s):
Fas apoptotic inhibitory molecule 2
Neural membrane protein 35
Transmembrane BAX inhibitor motif-containing protein 2
Gene names
Name:FAIM2
Synonyms:KIAA0950, LFG, LFG2, NMP35, TMBIM2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. May play a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development. Ref.1 Ref.7

Subunit structure

Interacts with FAS/TNFRSF6 and BAX. Ref.1 Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Membrane raft. Cell junctionsynapsepostsynaptic cell membrane By similarity Ref.7.

Tissue specificity

Highly expressed in breast carcinoma tissues. Enhanced expression correlates with the grade of the tumor (grade II/grade III) in primary breast tumors (at protein level). Widely expressed. Expressed at high levels in the brain especially in the hippocampus. Ref.1 Ref.10

Induction

Regulated by the AKT1/LEF1 pathway in breast cancer cell lines. Ref.9

Sequence similarities

Belongs to the BI1 family. LFG subfamily.

Sequence caution

The sequence AAF06327.1 differs from that shown. Reason: Frameshift at positions 82 and 130.

The sequence BAA76794.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Protein lifeguard 2
PRO_0000179087

Regions

Transmembrane106 – 12621Helical; Potential
Transmembrane138 – 15821Helical; Potential
Transmembrane165 – 18521Helical; Potential
Transmembrane194 – 21421Helical; Potential
Transmembrane225 – 24521Helical; Potential
Transmembrane250 – 27021Helical; Potential
Transmembrane290 – 31021Helical; Potential
Compositional bias71 – 755Poly-Ser

Amino acid modifications

Glycosylation1911N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict31Q → R in AAF06327. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9BWQ8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 574128C7ADCC51C0

FASTA31635,110
        10         20         30         40         50         60 
MTQGKLSVAN KAPGTEGQQQ VHGEKKEAPA VPSAPPSYEE ATSGEGMKAG AFPPAPTAVP 

        70         80         90        100        110        120 
LHPSWAYVDP SSSSSYDNGF PTGDHELFTT FSWDDQKVRR VFVRKVYTIL LIQLLVTLAV 

       130        140        150        160        170        180 
VALFTFCDPV KDYVQANPGW YWASYAVFFA TYLTLACCSG PRRHFPWNLI LLTVFTLSMA 

       190        200        210        220        230        240 
YLTGMLSSYY NTTSVLLCLG ITALVCLSVT VFSFQTKFDF TSCQGVLFVL LMTLFFSGLI 

       250        260        270        280        290        300 
LAILLPFQYV PWLHAVYAAL GAGVFTLFLA LDTQLLMGNR RHSLSPEEYI FGALNIYLDI 

       310 
IYIFTFFLQL FGTNRE 

« Hide

References

« Hide 'large scale' references
[1]"LFG: an anti-apoptotic gene that provides protection from fas-mediated cell death."
Somia N.V., Schmitt M.J., Vetter D.E., Van Antwerp D., Heinemann S.F., Verma I.M.
Proc. Natl. Acad. Sci. U.S.A. 96:12667-12672(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH FAS/TNFRSF6, TISSUE SPECIFICITY.
Tissue: Lung fibroblast.
[2]"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Hippocampus.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Sequence analysis shows that Lifeguard belongs to a new evolutionarily conserved cytoprotective family."
Reimers K., Choi C.Y., Mau-Thek E., Vogt P.M.
Int. J. Mol. Med. 18:729-734(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, INTERACTION WITH BAX.
[7]"Lifeguard/neuronal membrane protein 35 regulates Fas ligand-mediated apoptosis in neurons via microdomain recruitment."
Fernandez M., Segura M.F., Sole C., Colino A., Comella J.X., Cena V.
J. Neurochem. 103:190-203(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"LFG: a candidate apoptosis regulatory gene family."
Hu L., Smith T.F., Goldberger G.
Apoptosis 14:1255-1265(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[9]"Transactivation of lifeguard (LFG) by Akt-/LEF-1 pathway in MCF-7 and MDA-MB 231 human breast cancer cells."
Bucan V., Adili M.Y., Choi C.Y., Eddy M.T., Vogt P.M., Reimers K.
Apoptosis 15:814-821(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[10]"The anti-apoptotic protein lifeguard is expressed in breast cancer cells and tissues."
Bucan V., Reimers K., Choi C.Y., Eddy M.T., Vogt P.M.
Cell. Mol. Biol. Lett. 15:296-310(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF190461 mRNA. Translation: AAF06327.1. Frameshift.
AB023167 mRNA. Translation: BAA76794.1. Different initiation.
AK290031 mRNA. Translation: BAF82720.1.
CH471111 Genomic DNA. Translation: EAW58103.1.
BC000051 mRNA. Translation: AAH00051.1.
RefSeqNP_036438.2. NM_012306.3.
UniGeneHs.567424.

3D structure databases

ProteinModelPortalQ9BWQ8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116659. 1 interaction.
STRING9606.ENSP00000321951.

PTM databases

PhosphoSiteQ9BWQ8.

Polymorphism databases

DMDM38503167.

Proteomic databases

PaxDbQ9BWQ8.
PRIDEQ9BWQ8.

Protocols and materials databases

DNASU23017.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000320634; ENSP00000321951; ENSG00000135472.
GeneID23017.
KEGGhsa:23017.
UCSCuc001rvi.2. human.

Organism-specific databases

CTD23017.
GeneCardsGC12M050260.
HGNCHGNC:17067. FAIM2.
HPAHPA018790.
HPA048800.
MIM604306. gene.
neXtProtNX_Q9BWQ8.
PharmGKBPA134879081.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0670.
HOGENOMHOG000060393.
HOVERGENHBG012084.
InParanoidQ9BWQ8.
OMASCQGVIF.
OrthoDBEOG7VB2G8.
PhylomeDBQ9BWQ8.
TreeFamTF319996.

Gene expression databases

ArrayExpressQ9BWQ8.
BgeeQ9BWQ8.
CleanExHS_FAIM2.
GenevestigatorQ9BWQ8.

Family and domain databases

InterProIPR006214. Bax_inhibitor_1-related.
[Graphical view]
PANTHERPTHR23291. PTHR23291. 1 hit.
PfamPF01027. Bax1-I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFAIM2. human.
GenomeRNAi23017.
NextBio43956.
PROQ9BWQ8.
SOURCESearch...

Entry information

Entry nameLFG2_HUMAN
AccessionPrimary (citable) accession number: Q9BWQ8
Secondary accession number(s): A8K1W6, Q9UJY9, Q9Y2F7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM