Q9BWQ8 (LFG2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein lifeguard 2

Alternative name(s):
Fas apoptotic inhibitory molecule 2
Neural membrane protein 35
Transmembrane BAX inhibitor motif-containing protein 2

Gene names

Name:	FAIM2
Synonyms:	KIAA0950, LFG, LFG2, NMP35, TMBIM2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Antiapoptotic protein which protects cells uniquely from Fas-induced apoptosis. Regulates Fas-mediated apoptosis in neurons by interfering with caspase-8 activation. May play a role in cerebellar development by affecting cerebellar size, internal granular layer (IGL) thickness, and Purkinje cell (PC) development. Ref.1 Ref.7
Subunit structure	Interacts with FAS/TNFRSF6 and BAX. Ref.1 Ref.6
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Membrane raft. Cell junction › synapse › postsynaptic cell membrane By similarity Ref.7.
Tissue specificity	Highly expressed in breast carcinoma tissues. Enhanced expression correlates with the grade of the tumor (grade II/grade III) in primary breast tumors (at protein level). Widely expressed. Expressed at high levels in the brain especially in the hippocampus. Ref.1 Ref.10
Induction	Regulated by the AKT1/LEF1 pathway in breast cancer cell lines. Ref.9
Sequence similarities	Belongs to the BI1 family. LFG subfamily.
Sequence caution	The sequence AAF06327.1 differs from that shown. Reason: Frameshift at positions 82 and 130. The sequence BAA76794.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cerebellar Purkinje cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB cerebellar granular layer development Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of apoptotic process Non-traceable author statement Ref.1. Source: UniProtKB regulation of neuron apoptotic process Inferred from mutant phenotype Ref.7. Source: UniProtKB
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from direct assay Ref.7. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Protein lifeguard 2		PRO_0000179087
Regions
	Transmembrane	106 – 126	21	Helical; Potential
	Transmembrane	138 – 158	21	Helical; Potential
	Transmembrane	165 – 185	21	Helical; Potential
	Transmembrane	194 – 214	21	Helical; Potential
	Transmembrane	225 – 245	21	Helical; Potential
	Transmembrane	250 – 270	21	Helical; Potential
	Transmembrane	290 – 310	21	Helical; Potential
	Compositional bias	71 – 75	5	Poly-Ser
Amino acid modifications
	Modified residue	38	1	Phosphotyrosine By similarity
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	3	1	Q → R in AAF06327. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9BWQ8 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 574128C7ADCC51C0	FASTA	316	35,110
	10 20 30 40 50 60 MTQGKLSVAN KAPGTEGQQQ VHGEKKEAPA VPSAPPSYEE ATSGEGMKAG AFPPAPTAVP 70 80 90 100 110 120 LHPSWAYVDP SSSSSYDNGF PTGDHELFTT FSWDDQKVRR VFVRKVYTIL LIQLLVTLAV 130 140 150 160 170 180 VALFTFCDPV KDYVQANPGW YWASYAVFFA TYLTLACCSG PRRHFPWNLI LLTVFTLSMA 190 200 210 220 230 240 YLTGMLSSYY NTTSVLLCLG ITALVCLSVT VFSFQTKFDF TSCQGVLFVL LMTLFFSGLI 250 260 270 280 290 300 LAILLPFQYV PWLHAVYAAL GAGVFTLFLA LDTQLLMGNR RHSLSPEEYI FGALNIYLDI 310 IYIFTFFLQL FGTNRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LFG: an anti-apoptotic gene that provides protection from fas-mediated cell death." Somia N.V., Schmitt M.J., Vetter D.E., Van Antwerp D., Heinemann S.F., Verma I.M. Proc. Natl. Acad. Sci. U.S.A. 96:12667-12672(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH FAS/TNFRSF6, TISSUE SPECIFICITY. Tissue: Lung fibroblast.
[2]	"Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 6:63-70(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Sequence analysis shows that Lifeguard belongs to a new evolutionarily conserved cytoprotective family." Reimers K., Choi C.Y., Mau-Thek E., Vogt P.M. Int. J. Mol. Med. 18:729-734(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, INTERACTION WITH BAX.
[7]	"Lifeguard/neuronal membrane protein 35 regulates Fas ligand-mediated apoptosis in neurons via microdomain recruitment." Fernandez M., Segura M.F., Sole C., Colino A., Comella J.X., Cena V. J. Neurochem. 103:190-203(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"LFG: a candidate apoptosis regulatory gene family." Hu L., Smith T.F., Goldberger G. Apoptosis 14:1255-1265(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[9]	"Transactivation of lifeguard (LFG) by Akt-/LEF-1 pathway in MCF-7 and MDA-MB 231 human breast cancer cells." Bucan V., Adili M.Y., Choi C.Y., Eddy M.T., Vogt P.M., Reimers K. Apoptosis 15:814-821(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[10]	"The anti-apoptotic protein lifeguard is expressed in breast cancer cells and tissues." Bucan V., Reimers K., Choi C.Y., Eddy M.T., Vogt P.M. Cell. Mol. Biol. Lett. 15:296-310(2010) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF190461 mRNA. Translation: AAF06327.1. Frameshift. AB023167 mRNA. Translation: BAA76794.1. Different initiation. AK290031 mRNA. Translation: BAF82720.1. CH471111 Genomic DNA. Translation: EAW58103.1. BC000051 mRNA. Translation: AAH00051.1.
IPI	IPI00017569.
RefSeq	NP_036438.2. NM_012306.3.
UniGene	Hs.567424.
3D structure databases
ProteinModelPortal	Q9BWQ8.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000321951.
PTM databases
PhosphoSite	Q9BWQ8.
Polymorphism databases
DMDM	38503167.
Proteomic databases
PaxDb	Q9BWQ8.
PRIDE	Q9BWQ8.
Protocols and materials databases
DNASU	23017.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000320634; ENSP00000321951; ENSG00000135472. ENST00000550635; ENSP00000449711; ENSG00000135472.
GeneID	23017.
KEGG	hsa:23017.
UCSC	uc001rvi.2. human.
Organism-specific databases
CTD	23017.
GeneCards	GC12M050260.
HGNC	HGNC:17067. FAIM2.
HPA	HPA018790.
MIM	604306. gene.
neXtProt	NX_Q9BWQ8.
PharmGKB	PA134879081.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0670.
HOGENOM	HOG000060393.
HOVERGEN	HBG012084.
InParanoid	Q9BWQ8.
OMA	MGNRRYS.
OrthoDB	EOG43R3N3.
PhylomeDB	Q9BWQ8.
Gene expression databases
ArrayExpress	Q9BWQ8.
Bgee	Q9BWQ8.
CleanEx	HS_FAIM2.
Genevestigator	Q9BWQ8.
GermOnline	ENSG00000135472. Homo sapiens.
Family and domain databases
InterPro	IPR006214. Bax_inhibitor_1-related. [Graphical view]
PANTHER	PTHR23291. PTHR23291. 1 hit.
Pfam	PF01027. Bax1-I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	FAIM2. human.
GenomeRNAi	23017.
NextBio	43956.
SOURCE	Search...

Entry information

Entry name

LFG2_HUMAN

Accession

Primary (citable) accession number: Q9BWQ8
Secondary accession number(s): A8K1W6, Q9UJY9, Q9Y2F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.