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Protein

Splicing factor 3B subunit 5

Gene

SF3B5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3B subunit 5
Short name:
SF3b5
Alternative name(s):
Pre-mRNA-splicing factor SF3b 10 kDa subunit
Gene namesi
Name:SF3B5
Synonyms:SF3B10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000169976.6.
HGNCiHGNC:21083. SF3B5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000169976.
PharmGKBiPA134951632.

Polymorphism and mutation databases

BioMutaiSF3B5.
DMDMi20140757.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002207572 – 86Splicing factor 3B subunit 5Add BLAST85

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1
Modified residuei9PhosphoserineCombined sources1
Modified residuei17N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BWJ5.
MaxQBiQ9BWJ5.
PaxDbiQ9BWJ5.
PeptideAtlasiQ9BWJ5.
PRIDEiQ9BWJ5.
TopDownProteomicsiQ9BWJ5.

PTM databases

iPTMnetiQ9BWJ5.
PhosphoSitePlusiQ9BWJ5.

Expressioni

Gene expression databases

BgeeiENSG00000169976.
CleanExiHS_SF3B5.
GenevisibleiQ9BWJ5. HS.

Organism-specific databases

HPAiHPA054408.

Interactioni

Subunit structurei

Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42 (PubMed:12234937, PubMed:12738865, PubMed:28541300, PubMed:27720643). SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP) (PubMed:12234937). Within the SF3B complex interacts directly with SF3B1 (via HEAT domain) and SF3B3 (PubMed:27720643). The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2 (PubMed:27720643). Component of the U11/U12 snRNPs that are part of the U12-type spliceosome (PubMed:15146077).5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei5Interaction with RNA1 Publication1
Sitei20Interaction with RNA1 Publication1

Protein-protein interaction databases

BioGridi123645. 34 interactors.
CORUMiQ9BWJ5.
IntActiQ9BWJ5. 8 interactors.
MINTiMINT-3062697.
STRINGi9606.ENSP00000356541.

Structurei

Secondary structure

186
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi28 – 45Combined sources18
Helixi47 – 56Combined sources10
Helixi61 – 70Combined sources10
Turni71 – 73Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IFEX-ray3.10B1-86[»]
5O9Zelectron microscopy4.50x1-86[»]
ProteinModelPortaliQ9BWJ5.
SMRiQ9BWJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 76Interaction with SF3B1 and SF3B31 PublicationAdd BLAST62

Sequence similaritiesi

Belongs to the SF3B5 family.Curated

Phylogenomic databases

eggNOGiKOG3485. Eukaryota.
ENOG4111NTV. LUCA.
GeneTreeiENSGT00390000013215.
HOVERGENiHBG054589.
InParanoidiQ9BWJ5.
KOiK12832.
OMAiQSKYVGT.
OrthoDBiEOG091G0ZLI.
PhylomeDBiQ9BWJ5.
TreeFamiTF300117.

Family and domain databases

InterProiView protein in InterPro
IPR009846. SF3b5/RDS3-10.
IPR017089. Splicing_factor_3B_subunit_5.
PANTHERiPTHR20978. PTHR20978. 1 hit.
PfamiView protein in Pfam
PF07189. SF3b10. 1 hit.
PIRSFiPIRSF037010. Splicing_factor_3B_subunit_5. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BWJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRYTIHSQ LEHLQSKYIG TGHADTTKWE WLVNQHRDSY CSYMGHFDLL
60 70 80
NYFAIAENES KARVRFNLME KMLQPCGPPA DKPEEN
Length:86
Mass (Da):10,135
Last modified:June 1, 2001 - v1
Checksum:iB8A209EA9F548E8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK312079 mRNA. Translation: BAG35015.1.
AL031390 Genomic DNA. No translation available.
CH471051 Genomic DNA. Translation: EAW47851.1.
BC000198 mRNA. Translation: AAH00198.1.
BK000562 Genomic DNA. Translation: DAA00073.1.
CCDSiCCDS5204.1.
RefSeqiNP_112577.1. NM_031287.2.
UniGeneiHs.110695.

Genome annotation databases

EnsembliENST00000367569; ENSP00000356541; ENSG00000169976.
GeneIDi83443.
KEGGihsa:83443.
UCSCiuc003qkr.2. human.

Similar proteinsi

Entry informationi

Entry nameiSF3B5_HUMAN
AccessioniPrimary (citable) accession number: Q9BWJ5
Secondary accession number(s): B2R568, Q7RTV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: June 1, 2001
Last modified: November 22, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries