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Protein

E3 ubiquitin-protein ligase TRAIP

Gene

TRAIP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase acting as a negative regulator of innate immune signaling. Inhibits activation of NF-kappa-B mediated by TNF. Negatively regulates TLR3/4- and RIG-I-mediated IRF3 activation and subsequent IFNB1 production and cellular antiviral response by promoting 'Lys-48'-linked polyubiquitination of TNK1 leading to its proteasomal degradation (By similarity) (PubMed:22945920). Involved in response to genotoxic lesions during genome replication. Promotes H2AX and RPA2 phosphorylation after replication-associated DNA damage and assists fork progression at UV-induced replication-blocking lesions during S phase (PubMed:26595769). Has also been proposed to play a role in promoting translesion synthesis by mediating the assembly of 'Lys-63'-linked poly-ubiquitin chains on the Y-family polymerase POLN in order to facilitate bypass of DNA lesions and preserve genomic integrity (PubMed:24553286). The function in translesion synthesis is controversial (PubMed:26595769).1 PublicationBy similarity3 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 50RING-typePROSITE-ProRule annotationAdd BLAST44

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: ProtInc
  • cell proliferation Source: ProtInc
  • negative regulation of interferon-beta production Source: Ensembl
  • negative regulation of tumor necrosis factor-mediated signaling pathway Source: CACAO
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
SignaLinkiQ9BWF2.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRAIP (EC:2.3.2.27)
Alternative name(s):
RING finger protein 206
RING-type E3 ubiquitin transferase TRAIPCurated
TRAF-interacting protein
Gene namesi
Name:TRAIP
Synonyms:RNF206, TRIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:30764. TRAIP.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Seckel syndrome 9 (SCKL9)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of Seckel syndrome, a rare autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly with a bird-headed like appearance, and mental retardation.
See also OMIM:616777
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07653018R → C in SCKL9. 1 PublicationCorresponds to variant dbSNP:rs864622784Ensembl.1

Keywords - Diseasei

Disease mutation, Dwarfism, Mental retardation

Organism-specific databases

DisGeNETi10293.
MalaCardsiTRAIP.
MIMi616777. phenotype.
OpenTargetsiENSG00000183763.
PharmGKBiPA142670705.

Polymorphism and mutation databases

BioMutaiTRAIP.
DMDMi30580637.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561911 – 469E3 ubiquitin-protein ligase TRAIPAdd BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki304Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Autoubiquitinated.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9BWF2.
MaxQBiQ9BWF2.
PaxDbiQ9BWF2.
PeptideAtlasiQ9BWF2.
PRIDEiQ9BWF2.

PTM databases

iPTMnetiQ9BWF2.
PhosphoSitePlusiQ9BWF2.

Expressioni

Gene expression databases

BgeeiENSG00000183763.
CleanExiHS_TRAIP.
ExpressionAtlasiQ9BWF2. baseline and differential.
GenevisibleiQ9BWF2. HS.

Organism-specific databases

HPAiHPA036261.
HPA036262.

Interactioni

Subunit structurei

Binds TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6 is part of the receptor-TRAF signaling complex (By similarity). The C-terminus interacts with CYLD (PubMed:14676304), however the interaction was not detected with the full-length protein. Interacts with POLK and POLN.By similarity2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115581. 37 interactors.
IntActiQ9BWF2. 17 interactors.
MINTiMINT-6781129.
STRINGi9606.ENSP00000328203.

Structurei

Secondary structure

1469
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi463 – 465Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ZTDX-ray2.20D/E459-469[»]
ProteinModelPortaliQ9BWF2.
SMRiQ9BWF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 469Interaction with CYLD1 PublicationAdd BLAST259

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili70 – 177Sequence analysisAdd BLAST108
Coiled coili201 – 280Sequence analysisAdd BLAST80

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 50RING-typePROSITE-ProRule annotationAdd BLAST44

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0827. Eukaryota.
ENOG410YY6J. LUCA.
GeneTreeiENSGT00390000007696.
HOGENOMiHOG000054214.
HOVERGENiHBG059760.
InParanoidiQ9BWF2.
KOiK11985.
OMAiKEKRDSQ.
OrthoDBiEOG091G0QPY.
PhylomeDBiQ9BWF2.
TreeFamiTF317309.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF13639. zf-RING_2. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BWF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIRALCTIC SDFFDHSRDV AAIHCGHTFH LQCLIQWFET APSRTCPQCR
60 70 80 90 100
IQVGKRTIIN KLFFDLAQEE ENVLDAEFLK NELDNVRAQL SQKDKEKRDS
110 120 130 140 150
QVIIDTLRDT LEERNATVVS LQQALGKAEM LCSTLKKQMK YLEQQQDETK
160 170 180 190 200
QAQEEARRLR SKMKTMEQIE LLLQSQRPEV EEMIRDMGVG QSAVEQLAVY
210 220 230 240 250
CVSLKKEYEN LKEARKASGE VADKLRKDLF SSRSKLQTVY SELDQAKLEL
260 270 280 290 300
KSAQKDLQSA DKEIMSLKKK LTMLQETLNL PPVASETVDR LVLESPAPVE
310 320 330 340 350
VNLKLRRPSF RDDIDLNATF DVDTPPARPS SSQHGYYEKL CLEKSHSPIQ
360 370 380 390 400
DVPKKICKGP RKESQLSLGG QSCAGEPDEE LVGAFPIFVR NAILGQKQPK
410 420 430 440 450
RPRSESSCSK DVVRTGFDGL GGRTKFIQPT DTVMIRPLPV KPKTKVKQRV
460
RVKTVPSLFQ AKLDTFLWS
Length:469
Mass (Da):53,294
Last modified:June 1, 2001 - v1
Checksum:iB9EF3808FBC5985B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37W → S in AAB52993 (PubMed:9104814).Curated1
Sequence conflicti76A → R in AAB52993 (PubMed:9104814).Curated1
Sequence conflicti157R → G in AAB52993 (PubMed:9104814).Curated1
Sequence conflicti177R → L in AAB52993 (PubMed:9104814).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_07653018R → C in SCKL9. 1 PublicationCorresponds to variant dbSNP:rs864622784Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77845 mRNA. Translation: AAB52993.1.
AB451320 mRNA. Translation: BAG70134.1.
AB451449 mRNA. Translation: BAG70263.1.
BC000310 mRNA. Translation: AAH00310.1.
BC019283 mRNA. Translation: AAH19283.1.
CCDSiCCDS2806.1.
RefSeqiNP_005870.2. NM_005879.2.
UniGeneiHs.517972.

Genome annotation databases

EnsembliENST00000331456; ENSP00000328203; ENSG00000183763.
GeneIDi10293.
KEGGihsa:10293.
UCSCiuc003cxs.2. human.

Similar proteinsi

Entry informationi

Entry nameiTRAIP_HUMAN
AccessioniPrimary (citable) accession number: Q9BWF2
Secondary accession number(s): B5BU84, B5BUL3, O00467
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: June 1, 2001
Last modified: August 30, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references