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Q9BW66 (CINP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 2-interacting protein

Short name=CDK2-interacting protein
Gene names
Name:CINP
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication. Regulates ATR-mediated checkpoint signaling. Ref.1 Ref.9

Subunit structure

Homodimer. Interacts with CDK2 and CDC7. Interacts with the components of the replication complex, MCM2, MCM3, MCM4, MCM5, MCM6, MCM7 and with ORC2-containing complexes. Interacts with ATRIP. Interacts with CEP152. Ref.1 Ref.9 Ref.11

Subcellular location

Nucleus. Note: Binds to nuclear under G1 conditions, and dissociates from chromatin with the start of DNA replication. Ref.9

Post-translational modification

Phosphorylated by CDC7 but not by CDK2. Ref.1

Sequence similarities

Belongs to the CINP family.

Sequence caution

The sequence BU174070 differs from that shown. Reason: Frameshift at positions 183 and 197.

Ontologies

Keywords
   Biological processCell cycle
Cell division
DNA damage
DNA repair
DNA replication
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainCoiled coil
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

cell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction Ref.11. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BW66-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BW66-2)

The sequence of this isoform differs from the canonical sequence as follows:
     103-118: TKIQVKMEKLSSTTKG → MRFRISSWRCTGRSCS
     119-212: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9BW66-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MEA → MNGTIYANECQQIRHPNS
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 212212Cyclin-dependent kinase 2-interacting protein
PRO_0000326055

Regions

Coiled coil73 – 10735 Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.12

Natural variations

Alternative sequence1 – 33MEA → MNGTIYANECQQIRHPNS in isoform 3.
VSP_046386
Alternative sequence103 – 11816TKIQV…STTKG → MRFRISSWRCTGRSCS in isoform 2.
VSP_045342
Alternative sequence119 – 21294Missing in isoform 2.
VSP_045343
Natural variant1641R → H.
Corresponds to variant rs7011 [ dbSNP | Ensembl ].
VAR_039979
Natural variant1771D → N in a colorectal cancer sample; somatic mutation. Ref.13
VAR_039980

Experimental info

Sequence conflict1571R → K in AAF44747. Ref.1
Sequence conflict1571R → K in AAF44748. Ref.1
Sequence conflict1751D → N in AAF44747. Ref.1
Sequence conflict1751D → N in AAF44748. Ref.1
Sequence conflict194 – 1974SDSR → NDNK in AAF44747. Ref.1
Sequence conflict194 – 1974SDSR → NDNK in AAF44748. Ref.1
Sequence conflict1961S → R in BU174070. Ref.6
Sequence conflict2021S → N in AAF44747. Ref.1
Sequence conflict2021S → N in AAF44748. Ref.1
Sequence conflict210 – 2123RAL → PSSLTS in BU174070. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 3678E123B8C206B1

FASTA21224,324
        10         20         30         40         50         60 
MEAKTLGTVT PRKPVLSVSA RKIKDNAADW HNLILKWETL NDAGFTTANN IANLKISLLN 

        70         80         90        100        110        120 
KDKIELDSSS PASKENEEKV CLEYNEELEK LCEELQATLD GLTKIQVKME KLSSTTKGIC 

       130        140        150        160        170        180 
ELENYHYGEE SKRPPLFHTW PTTHFYEVSH KLLEMYRKEL LLKRTVAKEL AHTGDPDLTL 

       190        200        210 
SYLSMWLHQP YVESDSRLHL ESMLLETGHR AL 

« Hide

Isoform 2 [UniParc].

Checksum: EBAB2978717852CE
Show »

FASTA11813,287
Isoform 3 [UniParc].

Checksum: 9245BA7C5767DA0B
Show »

FASTA22726,051

References

« Hide 'large scale' references
[1]"A novel Cdk2 interactor is phosphorylated by Cdc7 and associates with components of the replication complexes."
Grishina I., Lattes B.
Cell Cycle 4:1120-1126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, FUNCTION, INTERACTION WITH CDK2; CDC7; MCM2; MCM3; MCM4; MCM5; MCM6; MCM7 AND ORC2, PHOSPHORYLATION.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Amygdala.
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Pancreatic carcinoma and Skin.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Functional genomic screens identify CINP as a genome maintenance protein."
Lovejoy C.A., Xu X., Bansbach C.E., Glick G.G., Zhao R., Ye F., Sirbu B.M., Titus L.C., Shyr Y., Cortez D.
Proc. Natl. Acad. Sci. U.S.A. 106:19304-19309(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ATRIP, SUBCELLULAR LOCATION.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"CEP152 is a genome maintenance protein disrupted in Seckel syndrome."
Kalay E., Yigit G., Aslan Y., Brown K.E., Pohl E., Bicknell L.S., Kayserili H., Li Y., Tuysuz B., Nurnberg G., Kiess W., Koegl M., Baessmann I., Buruk K., Toraman B., Kayipmaz S., Kul S., Ikbal M. expand/collapse author list , Turner D.J., Taylor M.S., Aerts J., Scott C., Milstein K., Dollfus H., Wieczorek D., Brunner H.G., Hurles M., Jackson A.P., Rauch A., Nurnberg P., Karaguzel A., Wollnik B.
Nat. Genet. 43:23-26(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CEP152.
[12]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ASN-177.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF228148 mRNA. Translation: AAF44747.1.
AF228149 mRNA. Translation: AAF44748.1.
AK056112 mRNA. Translation: BAB71095.1.
DA167716 mRNA. No translation available.
CR457178 mRNA. Translation: CAG33459.1.
AL137229 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81785.1.
BC000600 mRNA. Translation: AAH00600.1.
BU174070 mRNA. No translation available.
CCDSCCDS9972.1. [Q9BW66-1]
RefSeqNP_116019.1. NM_032630.2. [Q9BW66-1]
XP_005267810.1. XM_005267753.1. [Q9BW66-2]
UniGeneHs.129634.

3D structure databases

ProteinModelPortalQ9BW66.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119604. 5 interactions.
DIPDIP-49011N.
IntActQ9BW66. 3 interactions.
STRING9606.ENSP00000216756.

PTM databases

PhosphoSiteQ9BW66.

Polymorphism databases

DMDM74733401.

Proteomic databases

MaxQBQ9BW66.
PaxDbQ9BW66.
PRIDEQ9BW66.

Protocols and materials databases

DNASU51550.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000216756; ENSP00000216756; ENSG00000100865. [Q9BW66-1]
ENST00000536961; ENSP00000442057; ENSG00000100865. [Q9BW66-3]
ENST00000541568; ENSP00000442377; ENSG00000100865. [Q9BW66-2]
ENST00000559514; ENSP00000453839; ENSG00000100865. [Q9BW66-1]
GeneID51550.
KEGGhsa:51550.
UCSCuc001ylv.1. human. [Q9BW66-1]

Organism-specific databases

CTD51550.
GeneCardsGC14M102814.
HGNCHGNC:23789. CINP.
MIM613362. gene.
neXtProtNX_Q9BW66.
PharmGKBPA165478684.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43463.
HOGENOMHOG000035137.
HOVERGENHBG061504.
InParanoidQ9BW66.
OMALHLESML.
PhylomeDBQ9BW66.
TreeFamTF329462.

Gene expression databases

ArrayExpressQ9BW66.
BgeeQ9BW66.
GenevestigatorQ9BW66.

Family and domain databases

InterProIPR023250. Cyclin-dep_Kinase_2_interact.
[Graphical view]
PRINTSPR02040. CDK2IP.
ProtoNetSearch...

Other

GenomeRNAi51550.
NextBio55331.
PROQ9BW66.
SOURCESearch...

Entry information

Entry nameCINP_HUMAN
AccessionPrimary (citable) accession number: Q9BW66
Secondary accession number(s): F5H7P3, F5H8A7, Q9NPF9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM