Q9BW66 (CINP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 2-interacting protein
Short name=CDK2-interacting protein

Gene names

Name:

CINP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication. Regulates ATR-mediated checkpoint signaling. Ref.1 Ref.8
Subunit structure	Homodimer. Interacts with CDK2 and CDC7. Interacts with the components of the replication complex, MCM2, MCM3, MCM4, MCM5, MCM6, MCM7 and with ORC2-containing complexes. Interacts with ATRIP. Interacts with CEP152. Ref.1 Ref.8 Ref.10
Subcellular location	Nucleus. Note: Binds to nuclear under G1 conditions, and dissociates from chromatin with the start of DNA replication. Ref.8
Post-translational modification	Phosphorylated by CDC7 but not by CDK2. Ref.1
Sequence similarities	Belongs to the CINP family.
Sequence caution	The sequence BU174070 differs from that shown. Reason: Frameshift at positions 183 and 197.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA repair DNA replication
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BW66-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BW66-2)

The sequence of this isoform differs from the canonical sequence as follows:
     103-118: TKIQVKMEKLSSTTKG → MRFRISSWRCTGRSCS
     119-212: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9BW66-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MEA → MNGTIYANECQQIRHPNS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Cyclin-dependent kinase 2-interacting protein		PRO_0000326055
Regions
	Coiled coil	73 – 107	35	Potential
Natural variations
	Alternative sequence	1 – 3	3	MEA → MNGTIYANECQQIRHPNS in isoform 3.		VSP_046386
	Alternative sequence	103 – 118	16	TKIQV…STTKG → MRFRISSWRCTGRSCS in isoform 2.		VSP_045342
	Alternative sequence	119 – 212	94	Missing in isoform 2.		VSP_045343
	Natural variant	164	1	R → H. Corresponds to variant rs7011 [ dbSNP | Ensembl ].		VAR_039979
	Natural variant	177	1	D → N in a colorectal cancer sample; somatic mutation. Ref.11		VAR_039980
Experimental info
	Sequence conflict	157	1	R → K in AAF44747. Ref.1
	Sequence conflict	157	1	R → K in AAF44748. Ref.1
	Sequence conflict	175	1	D → N in AAF44747. Ref.1
	Sequence conflict	175	1	D → N in AAF44748. Ref.1
	Sequence conflict	194 – 197	4	SDSR → NDNK in AAF44747. Ref.1
	Sequence conflict	194 – 197	4	SDSR → NDNK in AAF44748. Ref.1
	Sequence conflict	196	1	S → R in BU174070. Ref.6
	Sequence conflict	202	1	S → N in AAF44747. Ref.1
	Sequence conflict	202	1	S → N in AAF44748. Ref.1
	Sequence conflict	210 – 212	3	RAL → PSSLTS in BU174070. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 3678E123B8C206B1	FASTA	212	24,324
	10 20 30 40 50 60 MEAKTLGTVT PRKPVLSVSA RKIKDNAADW HNLILKWETL NDAGFTTANN IANLKISLLN 70 80 90 100 110 120 KDKIELDSSS PASKENEEKV CLEYNEELEK LCEELQATLD GLTKIQVKME KLSSTTKGIC 130 140 150 160 170 180 ELENYHYGEE SKRPPLFHTW PTTHFYEVSH KLLEMYRKEL LLKRTVAKEL AHTGDPDLTL 190 200 210 SYLSMWLHQP YVESDSRLHL ESMLLETGHR AL
	Isoform 2 [UniParc]. Checksum: EBAB2978717852CE	FASTA	118	13,287
	10 20 30 40 50 60 MEAKTLGTVT PRKPVLSVSA RKIKDNAADW HNLILKWETL NDAGFTTANN IANLKISLLN 70 80 90 100 110 KDKIELDSSS PASKENEEKV CLEYNEELEK LCEELQATLD GLMRFRISSW RCTGRSCS
	Isoform 3 [UniParc]. Checksum: 9245BA7C5767DA0B	FASTA	227	26,051
	10 20 30 40 50 60 MNGTIYANEC QQIRHPNSKT LGTVTPRKPV LSVSARKIKD NAADWHNLIL KWETLNDAGF 70 80 90 100 110 120 TTANNIANLK ISLLNKDKIE LDSSSPASKE NEEKVCLEYN EELEKLCEEL QATLDGLTKI 130 140 150 160 170 180 QVKMEKLSST TKGICELENY HYGEESKRPP LFHTWPTTHF YEVSHKLLEM YRKELLLKRT 190 200 210 220 VAKELAHTGD PDLTLSYLSM WLHQPYVESD SRLHLESMLL ETGHRAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel Cdk2 interactor is phosphorylated by Cdc7 and associates with components of the replication complexes." Grishina I., Lattes B. Cell Cycle 4:1120-1126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, FUNCTION, INTERACTION WITH CDK2; CDC7; MCM2; MCM3; MCM4; MCM5; MCM6; MCM7 AND ORC2, PHOSPHORYLATION.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Amygdala.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Pancreatic carcinoma and Skin.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[8]	"Functional genomic screens identify CINP as a genome maintenance protein." Lovejoy C.A., Xu X., Bansbach C.E., Glick G.G., Zhao R., Ye F., Sirbu B.M., Titus L.C., Shyr Y., Cortez D. Proc. Natl. Acad. Sci. U.S.A. 106:19304-19309(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATRIP, SUBCELLULAR LOCATION.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"CEP152 is a genome maintenance protein disrupted in Seckel syndrome." Kalay E., Yigit G., Aslan Y., Brown K.E., Pohl E., Bicknell L.S., Kayserili H., Li Y., Tuysuz B., Nurnberg G., Kiess W., Koegl M., Baessmann I., Buruk K., Toraman B., Kayipmaz S., Kul S., Ikbal M. , Turner D.J., Taylor M.S., Aerts J., Scott C., Milstein K., Dollfus H., Wieczorek D., Brunner H.G., Hurles M., Jackson A.P., Rauch A., Nurnberg P., Karaguzel A., Wollnik B. Nat. Genet. 43:23-26(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CEP152.
[11]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ASN-177.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF228148 mRNA. Translation: AAF44747.1. AF228149 mRNA. Translation: AAF44748.1. AK056112 mRNA. Translation: BAB71095.1. DA167716 mRNA. No translation available. CR457178 mRNA. Translation: CAG33459.1. AL137229 Genomic DNA. No translation available. CH471061 Genomic DNA. Translation: EAW81785.1. BC000600 mRNA. Translation: AAH00600.1. BU174070 mRNA. No translation available.
IPI	IPI00013263. IPI00963950. IPI00964215.
RefSeq	NP_001171082.1. NM_001177611.1. NP_001171083.1. NM_001177612.1. NP_116019.1. NM_032630.2.
UniGene	Hs.129634.
3D structure databases
ProteinModelPortal	Q9BW66.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-49011N.
IntAct	Q9BW66. 2 interactions.
STRING	9606.ENSP00000216756.
PTM databases
PhosphoSite	Q9BW66.
Polymorphism databases
DMDM	74733401.
Proteomic databases
PaxDb	Q9BW66.
PRIDE	Q9BW66.
Protocols and materials databases
DNASU	51550.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000216756; ENSP00000216756; ENSG00000100865. ENST00000536961; ENSP00000442057; ENSG00000100865. ENST00000541568; ENSP00000442377; ENSG00000100865. ENST00000559514; ENSP00000453839; ENSG00000100865.
GeneID	51550.
KEGG	hsa:51550.
UCSC	uc001ylv.1. human.
Organism-specific databases
CTD	51550.
GeneCards	GC14M102814.
HGNC	HGNC:23789. CINP.
MIM	613362. gene.
neXtProt	NX_Q9BW66.
PharmGKB	PA165478684.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG43463.
HOGENOM	HOG000035137.
HOVERGEN	HBG061504.
InParanoid	Q9BW66.
OrthoDB	EOG4NGGNS.
Gene expression databases
ArrayExpress	Q9BW66.
Bgee	Q9BW66.
Genevestigator	Q9BW66.
Family and domain databases
InterPro	IPR023250. Cyclin-dep_Kinase_2_interact. [Graphical view]
PRINTS	PR02040. CDK2IP.
ProtoNet	Search...
Other
GenomeRNAi	51550.
NextBio	55331.
SOURCE	Search...

Entry information

Entry name

CINP_HUMAN

Accession

Primary (citable) accession number: Q9BW66
Secondary accession number(s): F5H7P3, F5H8A7, Q9NPF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.