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Protein

DET1- and DDB1-associated protein 1

Gene

DDA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in ubiquitination and subsequent proteasomal degradation of target proteins. Component of the DDD-E2 complexes which may provide a platform for interaction with CUL4A and WD repeat proteins.1 Publication

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
DET1- and DDB1-associated protein 1
Alternative name(s):
Placenta cross-immune reaction antigen 1
Short name:
PCIA-1
Gene namesi
Name:DDA1
Synonyms:C19orf58, PCIA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:28360. DDA1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162383418.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 102101DET1- and DDB1-associated protein 1PRO_0000310270Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei33 – 331Phosphoserine2 Publications
Modified residuei95 – 951Phosphoserine4 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9BW61.
PaxDbiQ9BW61.
PRIDEiQ9BW61.

PTM databases

PhosphoSiteiQ9BW61.

Expressioni

Gene expression databases

BgeeiQ9BW61.
CleanExiHS_DDA1.
ExpressionAtlasiQ9BW61. baseline and differential.
GenevestigatoriQ9BW61.

Organism-specific databases

HPAiHPA042134.
HPA055272.

Interactioni

Subunit structurei

Part of the DDD core complex containing DET1, DDA1 and DDB1. The DDD core complex recruits a specific UBE2E enzyme, such as UBE2E1, UBE2E2 UBE2E3, to form specific DDD-E2 complexes.1 Publication

Protein-protein interaction databases

BioGridi122485. 80 interactions.
DIPiDIP-53527N.
IntActiQ9BW61. 49 interactions.
STRINGi9606.ENSP00000352928.

Structurei

3D structure databases

ProteinModelPortaliQ9BW61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDA1 family.Curated

Phylogenomic databases

eggNOGiNOG264839.
GeneTreeiENSGT00390000007029.
HOGENOMiHOG000006384.
HOVERGENiHBG055081.
InParanoidiQ9BW61.
KOiK11792.
OMAiRTDSQDM.
OrthoDBiEOG7K3TP9.
PhylomeDBiQ9BW61.
TreeFamiTF323534.

Family and domain databases

InterProiIPR018276. Ub_ligase_Det1/DDB1-complexing.
[Graphical view]
PfamiPF10172. DDA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BW61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADFLKGLPV YNKSNFSRFH ADSVCKASNR RPSVYLPTRE YPSEQIIVTE
60 70 80 90 100
KTNILLRYLH QQWDKKNAAK KRDQEQVELE GESSAPPRKV ARTDSPDMHE

DT
Length:102
Mass (Da):11,835
Last modified:June 1, 2001 - v1
Checksum:i94E43C3528589F01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ090952 mRNA. Translation: AAZ41375.1.
AY563006 mRNA. Translation: AAS66629.1.
CH471106 Genomic DNA. Translation: EAW84593.1.
BC000615 mRNA. Translation: AAH00615.1.
CCDSiCCDS12357.1.
RefSeqiNP_076955.1. NM_024050.5.
UniGeneiHs.466154.

Genome annotation databases

EnsembliENST00000359866; ENSP00000352928; ENSG00000130311.
ENST00000593466; ENSP00000473086; ENSG00000130311.
ENST00000596582; ENSP00000472171; ENSG00000130311.
GeneIDi79016.
KEGGihsa:79016.
UCSCiuc002ngd.3. human.

Polymorphism databases

DMDMi74733400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ090952 mRNA. Translation: AAZ41375.1.
AY563006 mRNA. Translation: AAS66629.1.
CH471106 Genomic DNA. Translation: EAW84593.1.
BC000615 mRNA. Translation: AAH00615.1.
CCDSiCCDS12357.1.
RefSeqiNP_076955.1. NM_024050.5.
UniGeneiHs.466154.

3D structure databases

ProteinModelPortaliQ9BW61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122485. 80 interactions.
DIPiDIP-53527N.
IntActiQ9BW61. 49 interactions.
STRINGi9606.ENSP00000352928.

PTM databases

PhosphoSiteiQ9BW61.

Polymorphism databases

DMDMi74733400.

Proteomic databases

MaxQBiQ9BW61.
PaxDbiQ9BW61.
PRIDEiQ9BW61.

Protocols and materials databases

DNASUi79016.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359866; ENSP00000352928; ENSG00000130311.
ENST00000593466; ENSP00000473086; ENSG00000130311.
ENST00000596582; ENSP00000472171; ENSG00000130311.
GeneIDi79016.
KEGGihsa:79016.
UCSCiuc002ngd.3. human.

Organism-specific databases

CTDi79016.
GeneCardsiGC19P017420.
H-InvDBHIX0137478.
HGNCiHGNC:28360. DDA1.
HPAiHPA042134.
HPA055272.
neXtProtiNX_Q9BW61.
PharmGKBiPA162383418.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG264839.
GeneTreeiENSGT00390000007029.
HOGENOMiHOG000006384.
HOVERGENiHBG055081.
InParanoidiQ9BW61.
KOiK11792.
OMAiRTDSQDM.
OrthoDBiEOG7K3TP9.
PhylomeDBiQ9BW61.
TreeFamiTF323534.

Miscellaneous databases

ChiTaRSiDDA1. human.
GenomeRNAii79016.
NextBioi67674.
PROiQ9BW61.

Gene expression databases

BgeeiQ9BW61.
CleanExiHS_DDA1.
ExpressionAtlasiQ9BW61. baseline and differential.
GenevestigatoriQ9BW61.

Family and domain databases

InterProiIPR018276. Ub_ligase_Det1/DDB1-complexing.
[Graphical view]
PfamiPF10172. DDA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian DET1 regulates Cul4A Activity and forms stable complexes with E2 ubiquitin-conjugating enzymes."
    Pick E., Lau O.-S., Tsuge T., Menon S., Tong Y., Dohmae N., Plafker S.M., Deng X.W., Wei N.
    Mol. Cell. Biol. 27:4708-4719(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, IDENTIFICATION IN A DDD COMPLEX WITH DET1 AND DDB1.
  2. "Identification of placenta cross-immune reaction antigen."
    Deng H.-X., Wei Y.-Q., Zhao X., Yang H.-S., Yang J.-L., Tian L.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33 AND SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33 AND SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiDDA1_HUMAN
AccessioniPrimary (citable) accession number: Q9BW61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 1, 2001
Last modified: March 4, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.