Q9BW27 (NUP85_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear pore complex protein Nup85

Alternative name(s):
85 kDa nucleoporin
FROUNT
Nucleoporin Nup75
Nucleoporin Nup85
Pericentrin-1

Gene names

Name:	NUP85
Synonyms:	NUP75, PCNT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	656 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. Ref.2 Ref.7 Ref.9
Subunit structure	Component of the nuclear pore complex (NPC). Component of the NPC Nup107-160 subcomplex, consisting of at least NUP107, NUP98/Nup96, NUP160, NUP133, NUP85, NUP37, NUP43 and SEC13. Interacts with NUP160, NUP133 and SEC13. Interacts with NUP37, NUP107 and NUP43. Interacts with CCR2. Ref.1 Ref.2 Ref.7 Ref.8
Subcellular location	Nucleus › nuclear pore complex. Chromosome › centromere › kinetochore. Cytoplasm › cytoskeleton › spindle. Cytoplasm. Nucleus membrane. Note: During mitosis, localizes to the kinetochores and spindle poles. Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells. Ref.1 Ref.2 Ref.7 Ref.8 Ref.9
Sequence similarities	Belongs to the nucleoporin Nup85 family.
Sequence caution	The sequence CAD38749.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Kinetochore Membrane Nuclear pore complex Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement. Source: Reactome cytokine-mediated signaling pathway Traceable author statement. Source: Reactome glucose transport Traceable author statement. Source: Reactome lamellipodium assembly Inferred from electronic annotation. Source: Compara mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW macrophage chemotaxis Inferred from electronic annotation. Source: Compara mitotic prometaphase Traceable author statement. Source: Reactome protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of glucose transport Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome transmembrane transport Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear pore outer ring Inferred from direct assay Ref.8PubMed 17360435. Source: UniProtKB spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 656	656	Nuclear pore complex protein Nup85		PRO_0000324187
Amino acid modifications
	Modified residue	223	1	Phosphoserine Ref.10
Experimental info
	Sequence conflict	501	1	L → P in BAB14130. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9BW27 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 1AE2BA17D9CADF3D	FASTA	656	75,019
	10 20 30 40 50 60 MEELDGEPTV TLIPGVNSKK NQMYFDWGPG EMLVCETSFN KKEKSEMVPS CPFIYIIRKD 70 80 90 100 110 120 VDVYSQILRK LFNESHGIFL GLQRIDEELT GKSRKSQLVR VSKNYRSVIR ACMEEMHQVA 130 140 150 160 170 180 IAAKDPANGR QFSSQVSILS AMELIWNLCE ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL 190 200 210 220 230 240 SADVLGSENP SKHDSFWNLV TILVLQGRLD EARQMLSKEA DASPASAGIC RIMGDLMRTM 250 260 270 280 290 300 PILSPGNTQT LTELELKWQH WHEECERYLQ DSTFATSPHL ESLLKIMLGD EAALLEQKEL 310 320 330 340 350 360 LSNWYHFLVT RLLYSNPTVK PIDLHYYAQS SLDLFLGGES SPEPLDNILL AAFEFDIHQV 370 380 390 400 410 420 IKECSIALSN WWFVAHLTDL LDHCKLLQSH NLYFGSNMRE FLLLEYASGL FAHPSLWQLG 430 440 450 460 470 480 VDYFDYCPEL GRVSLELHIE RIPLNTEQKA LKVLRICEQR QMTEQVRSIC KILAMKAVRN 490 500 510 520 530 540 NRLGSALSWS IRAKDAAFAT LVSDRFLRDY CERGCFSDLD LIDNLGPAMM LSDRLTFLGK 550 560 570 580 590 600 YREFHRMYGE KRFADAASLL LSLMTSRIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL 610 620 630 640 650 MRCLEDLTSR RPVHGESDTE QLQDDDIETT KVEMLRLSLA RNLARAIIRE GSLEGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteomic analysis of the mammalian nuclear pore complex." Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J. J. Cell Biol. 158:915-927(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, IDENTIFICATION IN THE NUCLEAR PORE COMPLEX.
[2]	"Pivotal function for cytoplasmic protein FROUNT in CCR2-mediated monocyte chemotaxis." Terashima Y., Onai N., Murai M., Enomoto M., Poonpiriya V., Hamada T., Motomura K., Suwa M., Ezaki T., Haga T., Kanegasaki S., Matsushima K. Nat. Immunol. 6:827-835(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN CCR2 SIGNALING, INTERACTION WITH CCR2, SUBCELLULAR LOCATION.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[7]	"Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores." Harel A., Orjalo A.V., Vincent T., Lachish-Zalait A., Vasu S., Shah S., Zimmerman E., Elbaum M., Forbes D.J. Mol. Cell 11:853-864(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN NPC ASSEMBLY, SUBCELLULAR LOCATION, INTERACTION WITH NUP160; NUP133 AND SEC13.
[8]	"The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis." Loieodice I., Alves A., Rabut G., Van Overbeek M., Ellenberg J., Sibarita J.-B., Doye V. Mol. Biol. Cell 15:3333-3344(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE NUP107-160 COMPLEX, INTERACTION WITH NUP37; NUP107; NUP43 AND SEC13.
[9]	"The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly." Orjalo A.V., Arnaoutov A., Shen Z., Boyarchuk Y., Zeitlin S.G., Fontoura B., Briggs S., Dasso M., Forbes D.J. Mol. Biol. Cell 17:3806-3818(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN SPINDLE ASSSEMBLY, SUBCELLULAR LOCATION.
[10]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF514995 mRNA. Translation: AAM76706.1. AF498261 mRNA. Translation: AAM18528.1. AK022611 mRNA. Translation: BAB14130.1. AL833893 mRNA. Translation: CAD38749.1. Different initiation. CH471099 Genomic DNA. Translation: EAW89251.1. BC000697 mRNA. Translation: AAH00697.1.
IPI	IPI00790530.
RefSeq	NP_079120.1. NM_024844.3.
UniGene	Hs.362817.
3D structure databases
ProteinModelPortal	Q9BW27.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BW27. 5 interactions.
MINT	MINT-1408017.
STRING	9606.ENSP00000245544.
PTM databases
PhosphoSite	Q9BW27.
Polymorphism databases
DMDM	74733394.
Proteomic databases
PaxDb	Q9BW27.
PRIDE	Q9BW27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000245544; ENSP00000245544; ENSG00000125450.
GeneID	79902.
KEGG	hsa:79902.
UCSC	uc002jng.1. human.
Organism-specific databases
CTD	79902.
GeneCards	GC17P073201.
HGNC	HGNC:8734. NUP85.
MIM	170285. gene.
neXtProt	NX_Q9BW27.
PharmGKB	PA142671241.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG275863.
HOGENOM	HOG000230494.
HOVERGEN	HBG059778.
InParanoid	Q9BW27.
KO	K14304.
OMA	SICKIMA.
OrthoDB	EOG4229J7.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_115566. Cell Cycle. REACT_116125. Disease. REACT_15518. Transmembrane transport of small molecules. REACT_21300. Mitotic M-M/G1 phases. REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q9BW27.
Bgee	Q9BW27.
CleanEx	HS_NUP85.
Genevestigator	Q9BW27.
Family and domain databases
InterPro	IPR011502. Nucleoporin_Nup85. [Graphical view]
Pfam	PF07575. Nucleopor_Nup85. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NUP85. human.
GenomeRNAi	79902.
NextBio	69751.
SOURCE	Search...

Entry information

Entry name

NUP85_HUMAN

Accession

Primary (citable) accession number: Q9BW27
Secondary accession number(s): Q8NDI4, Q9H9U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.