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Q9BW27 (NUP85_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear pore complex protein Nup85
Alternative name(s):
85 kDa nucleoporin
FROUNT
Nucleoporin Nup75
Nucleoporin Nup85
Pericentrin-1
Gene names
Name:NUP85
Synonyms:NUP75, PCNT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length656 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. Ref.2 Ref.7 Ref.9

Subunit structure

Component of the nuclear pore complex (NPC). Component of the NPC Nup107-160 subcomplex, consisting of at least NUP107, NUP98/Nup96, NUP160, NUP133, NUP85, NUP37, NUP43 and SEC13. Interacts with NUP160, NUP133 and SEC13. Interacts with NUP37, NUP107 and NUP43. Interacts with CCR2. Ref.1 Ref.2 Ref.7 Ref.8

Subcellular location

Nucleusnuclear pore complex. Chromosomecentromerekinetochore. Cytoplasmcytoskeletonspindle. Cytoplasm. Nucleus membrane. Note: During mitosis, localizes to the kinetochores and spindle poles. Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells. Ref.1 Ref.2 Ref.7 Ref.8 Ref.9

Sequence similarities

Belongs to the nucleoporin Nup85 family.

Sequence caution

The sequence CAD38749.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processmRNA transport
Protein transport
Translocation
Transport
   Cellular componentCentromere
Chromosome
Cytoplasm
Cytoskeleton
Kinetochore
Membrane
Nuclear pore complex
Nucleus
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Traceable author statement. Source: Reactome

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

glucose transport

Traceable author statement. Source: Reactome

hexose transport

Traceable author statement. Source: Reactome

lamellipodium assembly

Inferred from electronic annotation. Source: Ensembl

mRNA transport

Inferred from electronic annotation. Source: UniProtKB-KW

macrophage chemotaxis

Inferred from electronic annotation. Source: Ensembl

mitotic cell cycle

Traceable author statement. Source: Reactome

mitotic nuclear envelope disassembly

Traceable author statement. Source: Reactome

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of glucose transport

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

transmembrane transport

Traceable author statement. Source: Reactome

viral process

Traceable author statement. Source: Reactome

   Cellular_componentcondensed chromosome kinetochore

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Traceable author statement. Source: Reactome

nuclear envelope

Traceable author statement. Source: Reactome

nuclear membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear pore outer ring

Inferred from direct assay Ref.8PubMed 17360435. Source: UniProtKB

spindle

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction Ref.8. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 656656Nuclear pore complex protein Nup85
PRO_0000324187

Amino acid modifications

Modified residue11N-acetylmethionine Ref.11
Modified residue921N6-acetyllysine By similarity
Modified residue2231Phosphoserine Ref.10

Experimental info

Sequence conflict5011L → P in BAB14130. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9BW27 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 1AE2BA17D9CADF3D

FASTA65675,019
        10         20         30         40         50         60 
MEELDGEPTV TLIPGVNSKK NQMYFDWGPG EMLVCETSFN KKEKSEMVPS CPFIYIIRKD 

        70         80         90        100        110        120 
VDVYSQILRK LFNESHGIFL GLQRIDEELT GKSRKSQLVR VSKNYRSVIR ACMEEMHQVA 

       130        140        150        160        170        180 
IAAKDPANGR QFSSQVSILS AMELIWNLCE ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL 

       190        200        210        220        230        240 
SADVLGSENP SKHDSFWNLV TILVLQGRLD EARQMLSKEA DASPASAGIC RIMGDLMRTM 

       250        260        270        280        290        300 
PILSPGNTQT LTELELKWQH WHEECERYLQ DSTFATSPHL ESLLKIMLGD EAALLEQKEL 

       310        320        330        340        350        360 
LSNWYHFLVT RLLYSNPTVK PIDLHYYAQS SLDLFLGGES SPEPLDNILL AAFEFDIHQV 

       370        380        390        400        410        420 
IKECSIALSN WWFVAHLTDL LDHCKLLQSH NLYFGSNMRE FLLLEYASGL FAHPSLWQLG 

       430        440        450        460        470        480 
VDYFDYCPEL GRVSLELHIE RIPLNTEQKA LKVLRICEQR QMTEQVRSIC KILAMKAVRN 

       490        500        510        520        530        540 
NRLGSALSWS IRAKDAAFAT LVSDRFLRDY CERGCFSDLD LIDNLGPAMM LSDRLTFLGK 

       550        560        570        580        590        600 
YREFHRMYGE KRFADAASLL LSLMTSRIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL 

       610        620        630        640        650 
MRCLEDLTSR RPVHGESDTE QLQDDDIETT KVEMLRLSLA RNLARAIIRE GSLEGS 

« Hide

References

« Hide 'large scale' references
[1]"Proteomic analysis of the mammalian nuclear pore complex."
Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J.
J. Cell Biol. 158:915-927(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, IDENTIFICATION IN THE NUCLEAR PORE COMPLEX.
[2]"Pivotal function for cytoplasmic protein FROUNT in CCR2-mediated monocyte chemotaxis."
Terashima Y., Onai N., Murai M., Enomoto M., Poonpiriya V., Hamada T., Motomura K., Suwa M., Ezaki T., Haga T., Kanegasaki S., Matsushima K.
Nat. Immunol. 6:827-835(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN CCR2 SIGNALING, INTERACTION WITH CCR2, SUBCELLULAR LOCATION.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[7]"Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores."
Harel A., Orjalo A.V., Vincent T., Lachish-Zalait A., Vasu S., Shah S., Zimmerman E., Elbaum M., Forbes D.J.
Mol. Cell 11:853-864(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN NPC ASSEMBLY, SUBCELLULAR LOCATION, INTERACTION WITH NUP160; NUP133 AND SEC13.
[8]"The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis."
Loieodice I., Alves A., Rabut G., Van Overbeek M., Ellenberg J., Sibarita J.-B., Doye V.
Mol. Biol. Cell 15:3333-3344(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE NUP107-160 COMPLEX, INTERACTION WITH NUP37; NUP107; NUP43 AND SEC13.
[9]"The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly."
Orjalo A.V., Arnaoutov A., Shen Z., Boyarchuk Y., Zeitlin S.G., Fontoura B., Briggs S., Dasso M., Forbes D.J.
Mol. Biol. Cell 17:3806-3818(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN SPINDLE ASSSEMBLY, SUBCELLULAR LOCATION.
[10]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF514995 mRNA. Translation: AAM76706.1.
AF498261 mRNA. Translation: AAM18528.1.
AK022611 mRNA. Translation: BAB14130.1.
AL833893 mRNA. Translation: CAD38749.1. Different initiation.
CH471099 Genomic DNA. Translation: EAW89251.1.
BC000697 mRNA. Translation: AAH00697.1.
CCDSCCDS32730.1.
RefSeqNP_079120.1. NM_024844.3.
UniGeneHs.362817.

3D structure databases

ProteinModelPortalQ9BW27.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122985. 19 interactions.
IntActQ9BW27. 15 interactions.
MINTMINT-1408017.
STRING9606.ENSP00000245544.

PTM databases

PhosphoSiteQ9BW27.

Polymorphism databases

DMDM74733394.

Proteomic databases

MaxQBQ9BW27.
PaxDbQ9BW27.
PRIDEQ9BW27.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000245544; ENSP00000245544; ENSG00000125450.
GeneID79902.
KEGGhsa:79902.
UCSCuc002jng.1. human.

Organism-specific databases

CTD79902.
GeneCardsGC17P073201.
HGNCHGNC:8734. NUP85.
HPAHPA062595.
MIM170285. gene.
neXtProtNX_Q9BW27.
PharmGKBPA142671241.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG275863.
HOGENOMHOG000230494.
HOVERGENHBG059778.
InParanoidQ9BW27.
KOK14304.
OMAHSLWQLA.
PhylomeDBQ9BW27.
TreeFamTF323240.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
REACT_115566. Cell Cycle.
REACT_116125. Disease.
REACT_15518. Transmembrane transport of small molecules.
REACT_21300. Mitotic M-M/G1 phases.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressQ9BW27.
BgeeQ9BW27.
CleanExHS_NUP85.
GenevestigatorQ9BW27.

Family and domain databases

InterProIPR011502. Nucleoporin_Nup85.
[Graphical view]
PfamPF07575. Nucleopor_Nup85. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNUP85. human.
GeneWikiNUP85.
GenomeRNAi79902.
NextBio69751.
PROQ9BW27.
SOURCESearch...

Entry information

Entry nameNUP85_HUMAN
AccessionPrimary (citable) accession number: Q9BW27
Secondary accession number(s): Q8NDI4, Q9H9U1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM