Q9BVS4 (RIOK2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase RIO2
EC=2.7.11.1

Alternative name(s):
RIO kinase 2

Gene names

Name:

RIOK2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BVS4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BVS4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     467-552: DEENVGAMNQ...SLEAASFWGE → YRLLSIAF

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Serine/threonine-protein kinase RIO2		PRO_0000213527
Regions
	Domain	271 – 552	282	Protein kinase
Sites
	Active site	228	1	Proton acceptor By similarity
	Binding site	123	1	ATP By similarity
Amino acid modifications
	Modified residue	332	1	Phosphoserine Ref.6 Ref.8 Ref.9 Ref.10
	Modified residue	335	1	Phosphoserine Ref.6 Ref.8 Ref.9 Ref.10
	Modified residue	337	1	Phosphoserine Ref.6 Ref.8 Ref.9 Ref.10 Ref.12
	Modified residue	350	1	Phosphoserine Ref.7
	Modified residue	362	1	Phosphoserine Ref.8
	Modified residue	380	1	Phosphoserine Ref.7 Ref.9
	Modified residue	382	1	Phosphoserine Ref.7 Ref.9
	Modified residue	385	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	390	1	Phosphoserine Ref.5 Ref.6 Ref.8 Ref.9
	Modified residue	412	1	Phosphoserine Ref.10
	Modified residue	417	1	Phosphoserine Ref.6
	Modified residue	442	1	Phosphoserine Ref.6 Ref.7 Ref.8
	Modified residue	445	1	Phosphotyrosine Ref.8
Natural variations
	Alternative sequence	467 – 552	86	DEENV…SFWGE → YRLLSIAF in isoform 2.		VSP_046388
	Natural variant	96	1	S → C. Ref.13 Corresponds to variant rs2544773 [ dbSNP | Ensembl ].		VAR_042347
	Natural variant	144	1	H → R. Ref.13 Corresponds to variant rs35165987 [ dbSNP | Ensembl ].		VAR_042348
	Natural variant	144	1	H → Y. Ref.4 Corresponds to variant rs17849382 [ dbSNP | Ensembl ].		VAR_031597
	Natural variant	155	1	R → H. Ref.13 Corresponds to variant rs34916955 [ dbSNP | Ensembl ].		VAR_042349
	Natural variant	175	1	V → I. Ref.13 Corresponds to variant rs35713904 [ dbSNP | Ensembl ].		VAR_042350
	Natural variant	216	1	I → T in a renal clear cell carcinoma sample; somatic mutation. Ref.13		VAR_042351
	Natural variant	244	1	M → V. Ref.13 Corresponds to variant rs33996030 [ dbSNP | Ensembl ].		VAR_042352
	Natural variant	349	1	G → R. Ref.1 Ref.13 Corresponds to variant rs160632 [ dbSNP | Ensembl ].		VAR_031598
	Natural variant	397	1	N → S. Ref.13 Corresponds to variant rs12188395 [ dbSNP | Ensembl ].		VAR_031599
	Natural variant	409	1	E → D. Ref.13 Corresponds to variant rs35829000 [ dbSNP | Ensembl ].		VAR_042353
	Natural variant	507	1	R → H. Ref.13 Corresponds to variant rs34555783 [ dbSNP | Ensembl ].		VAR_042354

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: 6EB260E72DDB78D7	FASTA	552	63,283
	10 20 30 40 50 60 MGKVNVAKLR YMSRDDFRVL TAVEMGMKNH EIVPGSLIAS IASLKHGGCN KVLRELVKHK 70 80 90 100 110 120 LIAWERTKTV QGYRLTNAGY DYLALKTLSS RQVVESVGNQ MGVGKESDIY IVANEEGQQF 130 140 150 160 170 180 ALKLHRLGRT SFRNLKNKRD YHKHRHNVSW LYLSRLSAMK EFAYMKALYE RKFPVPKPID 190 200 210 220 230 240 YNRHAVVMEL INGYPLCQIH HVEDPASVYD EAMELIVKLA NHGLIHGDFN EFNLILDESD 250 260 270 280 290 300 HITMIDFPQM VSTSHPNAEW YFDRDVKCIK DFFMKRFSYE SELFPTFKDI RREDTLDVEV 310 320 330 340 350 360 SASGYTKEMQ ADDELLHPLG PDDKNIETKE GSEFSFSDGE VAEKAEVYGS ENESERNCLE 370 380 390 400 410 420 ESEGCYCRSS GDPEQIKEDS LSEESADARS FEMTEFNQAL EEIKGQVVEN NSVTEFSEEK 430 440 450 460 470 480 NRTENYNRQD GQRVQGGVPA GSDEYEDECP HLIALSSLNR EFRPFRDEEN VGAMNQYRTR 490 500 510 520 530 540 TLSITSSGSA VSCSTIPPEL VKQKVKRQLT KQQKSAVRRR LQKGEANIFT KQRRENMQNI 550 KSSLEAASFW GE
	Isoform 2 [UniParc]. Checksum: 4B396021BE81BE84	FASTA	474	54,508
	10 20 30 40 50 60 MGKVNVAKLR YMSRDDFRVL TAVEMGMKNH EIVPGSLIAS IASLKHGGCN KVLRELVKHK 70 80 90 100 110 120 LIAWERTKTV QGYRLTNAGY DYLALKTLSS RQVVESVGNQ MGVGKESDIY IVANEEGQQF 130 140 150 160 170 180 ALKLHRLGRT SFRNLKNKRD YHKHRHNVSW LYLSRLSAMK EFAYMKALYE RKFPVPKPID 190 200 210 220 230 240 YNRHAVVMEL INGYPLCQIH HVEDPASVYD EAMELIVKLA NHGLIHGDFN EFNLILDESD 250 260 270 280 290 300 HITMIDFPQM VSTSHPNAEW YFDRDVKCIK DFFMKRFSYE SELFPTFKDI RREDTLDVEV 310 320 330 340 350 360 SASGYTKEMQ ADDELLHPLG PDDKNIETKE GSEFSFSDGE VAEKAEVYGS ENESERNCLE 370 380 390 400 410 420 ESEGCYCRSS GDPEQIKEDS LSEESADARS FEMTEFNQAL EEIKGQVVEN NSVTEFSEEK 430 440 450 460 470 NRTENYNRQD GQRVQGGVPA GSDEYEDECP HLIALSSLNR EFRPFRYRLL SIAF

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-349. Tissue: Placenta.
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Hepatoma.
[3]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT TYR-144. Tissue: Placenta.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-390, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332; SER-335; SER-337; SER-390; SER-417 AND SER-442, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350; SER-380; SER-382; SER-385 AND SER-442, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332; SER-335; SER-337; SER-362; SER-385; SER-390; SER-442 AND TYR-445, MASS SPECTROMETRY.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332; SER-335; SER-337; SER-380; SER-382; SER-385 AND SER-390, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332; SER-335; SER-337 AND SER-412, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337, MASS SPECTROMETRY.
[13]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] CYS-96; ARG-144; HIS-155; ILE-175; THR-216; VAL-244; ARG-349; SER-397; ASP-409 AND HIS-507.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK002021 mRNA. Translation: BAA92040.1. AK225348 mRNA. No translation available. AC008865 Genomic DNA. No translation available. AC008883 Genomic DNA. No translation available. BC000953 mRNA. Translation: AAH00953.1.
IPI	IPI00306406. IPI00930723.
RefSeq	NP_060813.2. NM_018343.2.
UniGene	Hs.27021.
3D structure databases
ProteinModelPortal	Q9BVS4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BVS4. 18 interactions.
STRING	9606.ENSP00000283109.
PTM databases
PhosphoSite	Q9BVS4.
Polymorphism databases
DMDM	143811448.
Proteomic databases
PaxDb	Q9BVS4.
PRIDE	Q9BVS4.
Protocols and materials databases
DNASU	55781.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000283109; ENSP00000283109; ENSG00000058729. ENST00000508447; ENSP00000420932; ENSG00000058729.
GeneID	55781.
KEGG	hsa:55781.
UCSC	uc003kmz.3. human.
Organism-specific databases
CTD	55781.
GeneCards	GC05M096496.
HGNC	HGNC:18999. RIOK2.
HPA	HPA005681.
neXtProt	NX_Q9BVS4.
PharmGKB	PA134885533.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0478.
HOGENOM	HOG000225685.
HOVERGEN	HBG056045.
InParanoid	Q9BVS4.
KO	K07179.
OMA	DECPDLV.
OrthoDB	EOG4W6NVN.
PhylomeDB	Q9BVS4.
Gene expression databases
ArrayExpress	Q9BVS4.
Bgee	Q9BVS4.
CleanEx	HS_RIOK2.
Genevestigator	Q9BVS4.
GermOnline	ENSG00000058729. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR018934. RIO-like_kinase. IPR015285. RIO2_kinase_winged_hlx_N. IPR018935. RIO_kinase_CS. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF01163. RIO1. 1 hit. PF09202. Rio2_N. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50011. PROTEIN_KINASE_DOM. False negative. PS01245. RIO1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9BVS4.
ChEMBL	CHEMBL6000.
GenomeRNAi	55781.
NextBio	60867.

Entry information

Entry name

RIOK2_HUMAN

Accession

Primary (citable) accession number: Q9BVS4
Secondary accession number(s): D6RDI3, Q9NUT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.