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Protein

Guanine nucleotide-binding protein-like 3

Gene

GNL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 181GTPSequence analysis4
Nucleotide bindingi261 – 268GTPSequence analysis8
Nucleotide bindingi305 – 308GTPSequence analysis4

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB
  • mRNA 5'-UTR binding Source: CAFA
  • RNA binding Source: UniProtKB

GO - Biological processi

  • cell proliferation Source: Ensembl
  • positive regulation of pri-miRNA transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of protein localization to chromosome, telomeric region Source: BHF-UCL
  • positive regulation of protein sumoylation Source: BHF-UCL
  • positive regulation of telomere maintenance Source: BHF-UCL
  • regulation of cell proliferation Source: UniProtKB
  • ribosome biogenesis Source: GO_Central
  • stem cell division Source: UniProtKB
  • stem cell population maintenance Source: UniProtKB

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein-like 3
Alternative name(s):
E2-induced gene 3 protein
Novel nucleolar protein 47
Short name:
NNP47
Nucleolar GTP-binding protein 3
Nucleostemin
Gene namesi
Name:GNL3
Synonyms:E2IG3, NS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000163938.16
HGNCiHGNC:29931 GNL3
MIMi608011 gene
neXtProtiNX_Q9BVP2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi26354
OpenTargetsiENSG00000163938
PharmGKBiPA134952132

Polymorphism and mutation databases

BioMutaiGNL3
DMDMi229462872

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224441 – 549Guanine nucleotide-binding protein-like 3Add BLAST549

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79N6-acetyllysineCombined sources1
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei101PhosphoserineBy similarity1
Cross-linki114Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki196Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki253Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki267Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki275Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei490PhosphoserineCombined sources1
Modified residuei504PhosphoserineBy similarity1
Modified residuei517PhosphoserineCombined sources1
Modified residuei529PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9BVP2
MaxQBiQ9BVP2
PaxDbiQ9BVP2
PeptideAtlasiQ9BVP2
PRIDEiQ9BVP2

2D gel databases

SWISS-2DPAGEQ9BVP2

PTM databases

iPTMnetiQ9BVP2
PhosphoSitePlusiQ9BVP2
SwissPalmiQ9BVP2

Expressioni

Tissue specificityi

Increased levels in lung tissue in cancer patients.1 Publication

Gene expression databases

BgeeiENSG00000163938
CleanExiHS_GNL3
ExpressionAtlasiQ9BVP2 baseline and differential
GenevisibleiQ9BVP2 HS

Organism-specific databases

HPAiCAB020770
HPA036742
HPA036743

Interactioni

Subunit structurei

Interacts with MDM2; this interaction stabilizes MDM2. Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly (By similarity). Indirectly interacts with TP53, via MDM2-binding (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi117690, 135 interactors
CORUMiQ9BVP2
IntActiQ9BVP2, 49 interactors
MINTiQ9BVP2
STRINGi9606.ENSP00000395772

Structurei

3D structure databases

ProteinModelPortaliQ9BVP2
SMRiQ9BVP2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 312CP-type GPROSITE-ProRule annotationAdd BLAST182

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 46BasicBy similarityAdd BLAST45
Regioni282 – 456IntermediateBy similarityAdd BLAST175
Regioni465 – 543AcidicBy similarityAdd BLAST79

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili56 – 95Sequence analysisAdd BLAST40

Domaini

The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis (By similarity).By similarity
In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2484 Eukaryota
COG1161 LUCA
GeneTreeiENSGT00810000125472
HOGENOMiHOG000207716
HOVERGENiHBG051747
InParanoidiQ9BVP2
KOiK14538
OMAiLLWSEWT
OrthoDBiEOG091G08IZ
PhylomeDBiQ9BVP2
TreeFamiTF313085

Family and domain databases

InterProiView protein in InterPro
IPR030378 G_CP_dom
IPR014813 Gnl3_N_dom
IPR006073 GTP_binding_domain
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF08701 GN3L_Grn1, 1 hit
PF01926 MMR_HSR1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51721 G_CP, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BVP2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV
60 70 80 90 100
PNSAPFKEAL LREAELRKQR LEELKQQQKL DRQKELEKKR KLETNPDIKP
110 120 130 140 150
SNVEPMEKEF GLCKTENKAK SGKQNSKKLY CQELKKVIEA SDVVLEVLDA
160 170 180 190 200
RDPLGCRCPQ VEEAIVQSGQ KKLVLILNKS DLVPKENLES WLNYLKKELP
210 220 230 240 250
TVVFRASTKP KDKGKITKRV KAKKNAAPFR SEVCFGKEGL WKLLGGFQET
260 270 280 290 300
CSKAIRVGVI GFPNVGKSSI INSLKQEQMC NVGVSMGLTR SMQVVPLDKQ
310 320 330 340 350
ITIIDSPSFI VSPLNSSSAL ALRSPASIEV VKPMEAASAI LSQADARQVV
360 370 380 390 400
LKYTVPGYRN SLEFFTVLAQ RRGMHQKGGI PNVEGAAKLL WSEWTGASLA
410 420 430 440 450
YYCHPPTSWT PPPYFNESIV VDMKSGFNLE ELEKNNAQSI RAIKGPHLAN
460 470 480 490 500
SILFQSSGLT NGIIEEKDIH EELPKRKERK QEEREDDKDS DQETVDEEVD
510 520 530 540
ENSSGMFAAE ETGEALSEET TAGEQSTRSF ILDKIIEEDD AYDFSTDYV
Length:549
Mass (Da):61,993
Last modified:May 5, 2009 - v2
Checksum:iF3928FE1C77C77F4
GO
Isoform 2 (identifier: Q9BVP2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: Missing.

Show »
Length:537
Mass (Da):60,540
Checksum:i342BCB22B4234056
GO

Sequence cautioni

The sequence AAF09482 differs from that shown. Reason: Frameshift at position 516.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3R → K in AAV74413 (PubMed:16012751).Curated1
Sequence conflicti86L → Q in AAV74413 (PubMed:16012751).Curated1
Sequence conflicti436N → D in AAV74413 (PubMed:16012751).Curated1
Sequence conflicti463I → M in BAB55169 (PubMed:14702039).Curated1
Sequence conflicti495V → A in AAV74413 (PubMed:16012751).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02216039R → Q3 PublicationsCorresponds to variant dbSNP:rs11177Ensembl.1
Natural variantiVAR_022161367V → MCombined sources3 PublicationsCorresponds to variant dbSNP:rs2289247Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0134111 – 12Missing in isoform 2. 1 PublicationAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF191018 mRNA Translation: AAF09482.1 Frameshift.
AY825265 mRNA Translation: AAV74413.1
AK027514 mRNA Translation: BAB55168.1
AK027516 mRNA Translation: BAB55169.1
AK315484 mRNA Translation: BAG37868.1
AC104446 Genomic DNA No translation available.
BC001024 mRNA Translation: AAH01024.1
CCDSiCCDS2861.1 [Q9BVP2-1]
CCDS43100.1 [Q9BVP2-2]
RefSeqiNP_055181.3, NM_014366.4 [Q9BVP2-1]
NP_996561.1, NM_206825.1 [Q9BVP2-2]
NP_996562.1, NM_206826.1 [Q9BVP2-2]
UniGeneiHs.313544

Genome annotation databases

EnsembliENST00000394799; ENSP00000378278; ENSG00000163938 [Q9BVP2-2]
ENST00000418458; ENSP00000395772; ENSG00000163938 [Q9BVP2-1]
GeneIDi26354
KEGGihsa:26354
UCSCiuc003dfd.4 human [Q9BVP2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiGNL3_HUMAN
AccessioniPrimary (citable) accession number: Q9BVP2
Secondary accession number(s): B2RDC1
, Q5PU80, Q96SV6, Q96SV7, Q9UJY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: May 5, 2009
Last modified: May 23, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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