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Protein

Transmembrane emp24 domain-containing protein 9

Gene

TMED9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 isoform PTPB.5 Publications

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • COPI coating of Golgi vesicle Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: Reactome
  • Golgi organization Source: UniProtKB
  • positive regulation of organelle organization Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 9
Alternative name(s):
GMP25
Glycoprotein 25L2
p24 family protein alpha-2
Short name:
p24alpha2
p25
Gene namesi
Name:TMED9
Synonyms:GP25L2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000184840.11
HGNCiHGNC:24878 TMED9
neXtProtiNX_Q9BVK6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 202LumenalSequence analysisAdd BLAST165
Transmembranei203 – 222HelicalSequence analysisAdd BLAST20
Topological domaini223 – 235CytoplasmicSequence analysisAdd BLAST13

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi232 – 233KK → SS: Localization to plasma membrane and endocytosis. 1 Publication2

Organism-specific databases

DisGeNETi54732
OpenTargetsiENSG00000184840
PharmGKBiPA134881976

Polymorphism and mutation databases

BioMutaiTMED9
DMDMi239938724

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37By similarityAdd BLAST37
ChainiPRO_000001039638 – 235Transmembrane emp24 domain-containing protein 9Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...) asparagine2 Publications1
Modified residuei160N6-acetyllysineBy similarity1

Post-translational modificationi

N-linked glycosylated containing high mannose.3 Publications

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

EPDiQ9BVK6
MaxQBiQ9BVK6
PaxDbiQ9BVK6
PeptideAtlasiQ9BVK6
PRIDEiQ9BVK6
ProteomicsDBi79214
TopDownProteomicsiQ9BVK6

PTM databases

iPTMnetiQ9BVK6
PhosphoSitePlusiQ9BVK6
SwissPalmiQ9BVK6

Expressioni

Gene expression databases

BgeeiENSG00000184840
CleanExiHS_TMED9
ExpressionAtlasiQ9BVK6 baseline and differential
GenevisibleiQ9BVK6 HS

Organism-specific databases

HPAiHPA014650

Interactioni

Subunit structurei

Monomer and homodimer in endoplasmic reticulum. Predominantly monomeric and to lesser extent homodimeric in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED10. Interacts with TMED5. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED9. Interacts with PTPN2 and SPAST. Interacts with STX17; the interaction is direct.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PTPN2P17706-15EBI-1056827,EBI-4409481

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi120115, 60 interactors
ELMiQ9BVK6
IntActiQ9BVK6, 27 interactors
MINTiQ9BVK6
STRINGi9606.ENSP00000330945

Structurei

3D structure databases

ProteinModelPortaliQ9BVK6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 145GOLDPROSITE-ProRule annotationAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 160Required for interaction with STX171 PublicationAdd BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili154 – 184Sequence analysisAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi228 – 235COPI vesicle coat-bindingSequence analysis8
Motifi228 – 229COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1690 Eukaryota
ENOG410XPUW LUCA
GeneTreeiENSGT00430000030848
HOGENOMiHOG000160228
HOVERGENiHBG105357
InParanoidiQ9BVK6
KOiK20346
OMAiVLWWSIV
OrthoDBiEOG091G0JDW
PhylomeDBiQ9BVK6
TreeFamiTF314123

Family and domain databases

InterProiView protein in InterPro
IPR009038 GOLD_dom
IPR015720 TMP21-related
PANTHERiPTHR22811 PTHR22811, 1 hit
PfamiView protein in Pfam
PF01105 EMP24_GP25L, 1 hit
SMARTiView protein in SMART
SM01190 EMP24_GP25L, 1 hit
PROSITEiView protein in PROSITE
PS50866 GOLD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BVK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVELGVLLV RPRPGTGLGR VMRTLLLVLW LATRGSALYF HIGETEKKCF
60 70 80 90 100
IEEIPDETMV IGNYRTQLYD KQREEYQPAT PGLGMFVEVK DPEDKVILAR
110 120 130 140 150
QYGSEGRFTF TSHTPGEHQI CLHSNSTKFS LFAGGMLRVH LDIQVGEHAN
160 170 180 190 200
DYAEIAAKDK LSELQLRVRQ LVEQVEQIQK EQNYQRWREE RFRQTSESTN
210 220 230
QRVLWWSILQ TLILVAIGVW QMRHLKSFFE AKKLV
Length:235
Mass (Da):27,277
Last modified:June 16, 2009 - v2
Checksum:i9B6D2D517D9E77D8
GO

Sequence cautioni

The sequence AAL35268 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA62380 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83L → F in CAA62380 (PubMed:15489334).Curated1
Sequence conflicti86F → C in CAA62380 (PubMed:15489334).Curated1
Sequence conflicti101Q → E in CAA62380 (PubMed:15489334).Curated1
Sequence conflicti102Y → C in AAL35268 (Ref. 3) Curated1
Sequence conflicti156A → P in CAA62380 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06117816T → S. Corresponds to variant dbSNP:rs57960711Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC139795 Genomic DNA No translation available.
BC001123 mRNA Translation: AAH01123.2
X90872 mRNA Translation: CAA62380.1 Different initiation.
AF441399 mRNA Translation: AAL35268.1 Different initiation.
CCDSiCCDS4428.1
RefSeqiNP_059980.2, NM_017510.5
UniGeneiHs.279929

Genome annotation databases

EnsembliENST00000332598; ENSP00000330945; ENSG00000184840
GeneIDi54732
KEGGihsa:54732
UCSCiuc003mhx.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTMED9_HUMAN
AccessioniPrimary (citable) accession number: Q9BVK6
Secondary accession number(s): Q14437, Q8WZ61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: June 16, 2009
Last modified: June 20, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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