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Protein

Transmembrane emp24 domain-containing protein 9

Gene

TMED9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 isoform PTPB.5 Publications

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • COPI coating of Golgi vesicle Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: Reactome
  • Golgi organization Source: UniProtKB
  • positive regulation of organelle organization Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • retrograde vesicle-mediated transport, Golgi to ER Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:G66-30847-MONOMER.
ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 9
Alternative name(s):
GMP25
Glycoprotein 25L2
p24 family protein alpha-2
Short name:
p24alpha2
p25
Gene namesi
Name:TMED9
Synonyms:GP25L2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:24878. TMED9.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 202LumenalSequence analysisAdd BLAST165
Transmembranei203 – 222HelicalSequence analysisAdd BLAST20
Topological domaini223 – 235CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  • endoplasmic reticulum-Golgi intermediate compartment membrane Source: Reactome
  • endoplasmic reticulum membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • trans-Golgi network transport vesicle Source: UniProtKB
  • transport vesicle Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi232 – 233KK → SS: Localization to plasma membrane and endocytosis. 1 Publication2

Organism-specific databases

DisGeNETi54732.
OpenTargetsiENSG00000184840.
PharmGKBiPA134881976.

Polymorphism and mutation databases

BioMutaiTMED9.
DMDMi239938724.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37By similarityAdd BLAST37
ChainiPRO_000001039638 – 235Transmembrane emp24 domain-containing protein 9Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...)2 Publications1
Modified residuei160N6-acetyllysineBy similarity1

Post-translational modificationi

N-linked glycosylated containing high mannose.3 Publications

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

EPDiQ9BVK6.
MaxQBiQ9BVK6.
PaxDbiQ9BVK6.
PeptideAtlasiQ9BVK6.
PRIDEiQ9BVK6.
TopDownProteomicsiQ9BVK6.

PTM databases

iPTMnetiQ9BVK6.
PhosphoSitePlusiQ9BVK6.
SwissPalmiQ9BVK6.

Expressioni

Gene expression databases

BgeeiENSG00000184840.
CleanExiHS_TMED9.
ExpressionAtlasiQ9BVK6. baseline and differential.
GenevisibleiQ9BVK6. HS.

Organism-specific databases

HPAiHPA014650.

Interactioni

Subunit structurei

Monomer and homodimer in endoplasmic reticulum. Predominantly monomeric and to lesser extent homodimeric in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED10. Interacts with TMED5. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED9. Interacts with PTPN2 and SPAST. Interacts with STX17; the interaction is direct.7 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PTPN2P17706-15EBI-1056827,EBI-4409481

GO - Molecular functioni

  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi120115. 50 interactors.
IntActiQ9BVK6. 25 interactors.
MINTiMINT-5001958.
STRINGi9606.ENSP00000330945.

Structurei

3D structure databases

ProteinModelPortaliQ9BVK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 145GOLDPROSITE-ProRule annotationAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni121 – 160Required for interaction with STX171 PublicationAdd BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili154 – 184Sequence analysisAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi228 – 235COPI vesicle coat-bindingSequence analysis8
Motifi228 – 229COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ9BVK6.
KOiK20346.
OMAiVLWWSIV.
OrthoDBiEOG091G0JDW.
PhylomeDBiQ9BVK6.
TreeFamiTF314123.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BVK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVELGVLLV RPRPGTGLGR VMRTLLLVLW LATRGSALYF HIGETEKKCF
60 70 80 90 100
IEEIPDETMV IGNYRTQLYD KQREEYQPAT PGLGMFVEVK DPEDKVILAR
110 120 130 140 150
QYGSEGRFTF TSHTPGEHQI CLHSNSTKFS LFAGGMLRVH LDIQVGEHAN
160 170 180 190 200
DYAEIAAKDK LSELQLRVRQ LVEQVEQIQK EQNYQRWREE RFRQTSESTN
210 220 230
QRVLWWSILQ TLILVAIGVW QMRHLKSFFE AKKLV
Length:235
Mass (Da):27,277
Last modified:June 16, 2009 - v2
Checksum:i9B6D2D517D9E77D8
GO

Sequence cautioni

The sequence AAL35268 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA62380 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83L → F in CAA62380 (PubMed:15489334).Curated1
Sequence conflicti86F → C in CAA62380 (PubMed:15489334).Curated1
Sequence conflicti101Q → E in CAA62380 (PubMed:15489334).Curated1
Sequence conflicti102Y → C in AAL35268 (Ref. 3) Curated1
Sequence conflicti156A → P in CAA62380 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06117816T → S.Corresponds to variant rs57960711dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC139795 Genomic DNA. No translation available.
BC001123 mRNA. Translation: AAH01123.2.
X90872 mRNA. Translation: CAA62380.1. Different initiation.
AF441399 mRNA. Translation: AAL35268.1. Different initiation.
CCDSiCCDS4428.1.
RefSeqiNP_059980.2. NM_017510.5.
UniGeneiHs.279929.

Genome annotation databases

EnsembliENST00000332598; ENSP00000330945; ENSG00000184840.
GeneIDi54732.
KEGGihsa:54732.
UCSCiuc003mhx.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC139795 Genomic DNA. No translation available.
BC001123 mRNA. Translation: AAH01123.2.
X90872 mRNA. Translation: CAA62380.1. Different initiation.
AF441399 mRNA. Translation: AAL35268.1. Different initiation.
CCDSiCCDS4428.1.
RefSeqiNP_059980.2. NM_017510.5.
UniGeneiHs.279929.

3D structure databases

ProteinModelPortaliQ9BVK6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120115. 50 interactors.
IntActiQ9BVK6. 25 interactors.
MINTiMINT-5001958.
STRINGi9606.ENSP00000330945.

PTM databases

iPTMnetiQ9BVK6.
PhosphoSitePlusiQ9BVK6.
SwissPalmiQ9BVK6.

Polymorphism and mutation databases

BioMutaiTMED9.
DMDMi239938724.

Proteomic databases

EPDiQ9BVK6.
MaxQBiQ9BVK6.
PaxDbiQ9BVK6.
PeptideAtlasiQ9BVK6.
PRIDEiQ9BVK6.
TopDownProteomicsiQ9BVK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000332598; ENSP00000330945; ENSG00000184840.
GeneIDi54732.
KEGGihsa:54732.
UCSCiuc003mhx.4. human.

Organism-specific databases

CTDi54732.
DisGeNETi54732.
GeneCardsiTMED9.
H-InvDBHIX0005470.
HGNCiHGNC:24878. TMED9.
HPAiHPA014650.
neXtProtiNX_Q9BVK6.
OpenTargetsiENSG00000184840.
PharmGKBiPA134881976.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1690. Eukaryota.
ENOG410XPUW. LUCA.
GeneTreeiENSGT00430000030848.
HOGENOMiHOG000160228.
HOVERGENiHBG105357.
InParanoidiQ9BVK6.
KOiK20346.
OMAiVLWWSIV.
OrthoDBiEOG091G0JDW.
PhylomeDBiQ9BVK6.
TreeFamiTF314123.

Enzyme and pathway databases

BioCyciZFISH:G66-30847-MONOMER.
ReactomeiR-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811434. COPI-dependent Golgi-to-ER retrograde traffic.

Miscellaneous databases

ChiTaRSiTMED9. human.
GenomeRNAii54732.
PROiQ9BVK6.

Gene expression databases

BgeeiENSG00000184840.
CleanExiHS_TMED9.
ExpressionAtlasiQ9BVK6. baseline and differential.
GenevisibleiQ9BVK6. HS.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMED9_HUMAN
AccessioniPrimary (citable) accession number: Q9BVK6
Secondary accession number(s): Q14437, Q8WZ61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.