Q9BVA0 (KTNB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Katanin p80 WD40-containing subunit B1
Short name=Katanin p80 subunit B1

Alternative name(s):
p80 katanin

Gene names

Name:

KATNB1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth By similarity. Ref.8
Subunit structure	Interacts with PAFAH1B1 By similarity. Interacts with KATNA1. This interaction enhances the microtubule binding and severing activity of KATNA1 and also targets this activity to the centrosome. Interacts with dynein, microtubules and NDEL1. Ref.8 Ref.9
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle pole. Cytoplasm › cytoskeleton. Note: Predominantly cytoplasmic. Localized to the interphase centrosome and mitotic spindle poles. Ref.1 Ref.7 Ref.9
Sequence similarities	Belongs to the WD repeat KATNB1 family. Contains 6 WD repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule
Coding sequence diversity	Polymorphism
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of microtubule depolymerization Inferred from mutant phenotype Ref.8. Source: UniProtKB positive regulation of microtubule depolymerization Inferred from mutant phenotype Ref.8. Source: UniProtKB protein targeting Non-traceable author statement Ref.8. Source: UniProtKB
Cellular component	katanin complex Traceable author statement Ref.8. Source: UniProtKB microtubule Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: HPA spindle pole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	microtubule binding Non-traceable author statement Ref.8. Source: UniProtKB protein heterodimerization activity Inferred from physical interaction Ref.8. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 655	655	Katanin p80 WD40-containing subunit B1		PRO_0000051049
Regions
	Repeat	18 – 58	41	WD 1
	Repeat	61 – 100	40	WD 2
	Repeat	103 – 142	40	WD 3
	Repeat	145 – 184	40	WD 4
	Repeat	187 – 226	40	WD 5
	Repeat	229 – 269	41	WD 6
	Region	1 – 300	300	Interaction with centrosomes
	Region	1 – 284	284	Interaction with dynein By similarity
	Region	285 – 434	150	Interaction with PAFAH1B1 By similarity
	Region	433 – 655	223	Interaction with KATNA1 and NDEL1 By similarity
Amino acid modifications
	Modified residue	395	1	Phosphothreonine Ref.10
Natural variations
	Natural variant	181	1	M → V. Corresponds to variant rs60158050 [ dbSNP | Ensembl ].		VAR_062099
Experimental info
	Sequence conflict	104	1	H → L in AAC09328. Ref.1
	Sequence conflict	211	1	R → G in AAC09328. Ref.1
	Sequence conflict	226	1	C → R in AAC09328. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9BVA0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 411903E306B6EC16	FASTA	655	72,334
	10 20 30 40 50 60 MATPVVTKTA WKLQEIVAHA SNVSSLVLGK ASGRLLATGG DDCRVNLWSI NKPNCIMSLT 70 80 90 100 110 120 GHTSPVESVR LNTPEELIVA GSQSGSIRVW DLEAAKILRT LMGHKANICS LDFHPYGEFV 130 140 150 160 170 180 ASGSQDTNIK LWDIRRKGCV FRYRGHSQAV RCLRFSPDGK WLASAADDHT VKLWDLTAGK 190 200 210 220 230 240 MMSEFPGHTG PVNVVEFHPN EYLLASGSSD RTIRFWDLEK FQVVSCIEGE PGPVRSVLFN 250 260 270 280 290 300 PDGCCLYSGC QDSLRVYGWE PERCFDVVLV NWGKVADLAI CNDQLIGVAF SQSNVSSYVV 310 320 330 340 350 360 DLTRVTRTGT VARDPVQDHR PLAQPLPNPS APLRRIYERP STTCSKPQRV KQNSESERRS 370 380 390 400 410 420 PSSEDDRDER ESRAEIQNAE DYNEIFQPKN SISRTPPRRS EPFPAPPEDD AATAKEAAKP 430 440 450 460 470 480 SPAMDVQFPV PNLEVLPRPP VVASTPAPKA EPAIIPATRN EPIGLKASDF LPAVKIPQQA 490 500 510 520 530 540 ELVDEDAMSQ IRKGHDTMCV VLTSRHKNLD TVRAVWTMGD IKTSVDSAVA INDLSVVVDL 550 560 570 580 590 600 LNIVNQKASL WKLDLCTTVL PQIEKLLQSK YESYVQTGCT SLKLILQRFL PLITDMLAAP 610 620 630 640 650 PSVGVDISRE ERLHKCRLCY KQLKSISGLV KSKSGLSGRH GSTFRELHLL MASLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit." Hartman J.J., Mahr J., McNally K., Okawa K., Iwamatsu A., Thomas S., Cheesman S., Heuser J., Vale R.D., McNally F.J. Cell 93:277-287(1998) [PubMed: 9568719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[7]	"Katanin is responsible for the M-phase microtubule-severing activity in Xenopus eggs." McNally F.J., Thomas S. Mol. Biol. Cell 9:1847-1861(1998) [PubMed: 9658175] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin." McNally K.P., Bazirgan O.A., McNally F.J. J. Cell Sci. 113:1623-1633(2000) [PubMed: 10751153] [Abstract] Cited for: FUNCTION, INTERACTION WITH KATNA1.
[9]	"Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration." Toyo-Oka K., Sasaki S., Yano Y., Mori D., Kobayashi T., Toyoshima Y.Y., Tokuoka S.M., Ishii S., Shimizu T., Muramatsu M., Hiraiwa N., Yoshiki A., Wynshaw-Boris A., Hirotsune S. Hum. Mol. Genet. 14:3113-3128(2005) [PubMed: 16203747] [Abstract] Cited for: INTERACTION WITH NDEL1, SUBCELLULAR LOCATION.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-395, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF052432 mRNA. Translation: AAC09328.1. BT007022 mRNA. Translation: AAP35668.1. CR456762 mRNA. Translation: CAG33043.1. AC092118 Genomic DNA. No translation available. CH471092 Genomic DNA. Translation: EAW82951.1. BC001353 mRNA. Translation: AAH01353.1.
IPI	IPI00746256.
RefSeq	NP_005877.2. NM_005886.2.
UniGene	Hs.275675.
3D structure databases
ProteinModelPortal	Q9BVA0.
SMR	Q9BVA0. Positions 11-300.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9BVA0.
PTM databases
PhosphoSite	Q9BVA0.
Polymorphism databases
DMDM	60390213.
Proteomic databases
PRIDE	Q9BVA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000268509; ENSP00000268509; ENSG00000140854. ENST00000379661; ENSP00000368982; ENSG00000140854.
GeneID	10300.
KEGG	hsa:10300.
UCSC	uc002eml.1. human.
Organism-specific databases
CTD	10300.
GeneCards	GC16P057770.
H-InvDB	HIX0013080.
HGNC	HGNC:6217. KATNB1.
HPA	HPA041165. HPA041839.
MIM	602703. gene.
neXtProt	NX_Q9BVA0.
PharmGKB	PA30018.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11815.
GeneTree	ENSGT00600000084025.
HOGENOM	HBG591364.
HOVERGEN	HBG052294.
InParanoid	Q9BVA0.
OMA	AVRCLRF.
OrthoDB	EOG470TGV.
PhylomeDB	Q9BVA0.
Gene expression databases
ArrayExpress	Q9BVA0.
Bgee	Q9BVA0.
CleanEx	HS_KATNB1.
Genevestigator	Q9BVA0.
GermOnline	ENSG00000140854. Homo sapiens.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 6 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 3 hits. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	39044.
SOURCE	Search...

Entry information

Entry name

KTNB1_HUMAN

Accession

Primary (citable) accession number: Q9BVA0
Secondary accession number(s): A6NCG6, O60620

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.