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Q9BVA0 (KTNB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Katanin p80 WD40 repeat-containing subunit B1

Short name=Katanin p80 subunit B1
Alternative name(s):
p80 katanin
Gene names
Name:KATNB1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth By similarity. Ref.8

Subunit structure

Interacts with PAFAH1B1 By similarity. Interacts with KATNA1. This interaction enhances the microtubule binding and severing activity of KATNA1 and also targets this activity to the centrosome. Interacts with dynein, microtubules and NDEL1. Ref.8 Ref.9

Subcellular location

Cytoplasm. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome. Cytoplasmcytoskeletonspindle pole. Cytoplasmcytoskeleton. Note: Predominantly cytoplasmic. Localized to the interphase centrosome and mitotic spindle poles. Ref.1 Ref.7 Ref.9

Sequence similarities

Belongs to the WD repeat KATNB1 family.

Contains 6 WD repeats.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
   Coding sequence diversityPolymorphism
   DomainRepeat
WD repeat
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from direct assay Ref.8. Source: GOC

microtubule severing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

mitotic chromosome movement towards spindle pole

Inferred from electronic annotation. Source: UniProtKB-HAMAP

negative regulation of microtubule depolymerization

Inferred from mutant phenotype Ref.8. Source: UniProtKB

positive regulation of cell death

Inferred from electronic annotation. Source: Ensembl

positive regulation of microtubule depolymerization

Inferred from mutant phenotype Ref.8. Source: UniProtKB

positive regulation of neuron projection development

Inferred from electronic annotation. Source: Ensembl

protein targeting

Non-traceable author statement Ref.8. Source: UniProtKB

   Cellular_componentaxon

Inferred from electronic annotation. Source: Ensembl

centrosome

Inferred from electronic annotation. Source: UniProtKB-HAMAP

cytoplasm

Inferred from direct assay Ref.8. Source: UniProtKB

growth cone

Inferred from electronic annotation. Source: Ensembl

katanin complex

Traceable author statement Ref.8. Source: UniProtKB

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

midbody

Inferred from electronic annotation. Source: Ensembl

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 21630459. Source: UniProt

spindle pole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmicrotubule binding

Non-traceable author statement Ref.8. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction Ref.8. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 655655Katanin p80 WD40 repeat-containing subunit B1 HAMAP-Rule MF_03022
PRO_0000051049

Regions

Repeat18 – 5841WD 1 HAMAP-Rule MF_03022
Repeat61 – 10040WD 2 HAMAP-Rule MF_03022
Repeat103 – 14240WD 3 HAMAP-Rule MF_03022
Repeat145 – 18440WD 4 HAMAP-Rule MF_03022
Repeat187 – 22640WD 5 HAMAP-Rule MF_03022
Repeat229 – 26941WD 6 HAMAP-Rule MF_03022
Region1 – 300300Interaction with centrosomes HAMAP-Rule MF_03022
Region1 – 284284Interaction with dynein By similarity
Region285 – 434150Interaction with PAFAH1B1 By similarity
Region433 – 655223Interaction with KATNA1 and NDEL1 By similarity

Natural variations

Natural variant1811M → V.
Corresponds to variant rs60158050 [ dbSNP | Ensembl ].
VAR_062099

Experimental info

Sequence conflict1041H → L in AAC09328. Ref.1
Sequence conflict2111R → G in AAC09328. Ref.1
Sequence conflict2261C → R in AAC09328. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9BVA0 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 411903E306B6EC16

FASTA65572,334
        10         20         30         40         50         60 
MATPVVTKTA WKLQEIVAHA SNVSSLVLGK ASGRLLATGG DDCRVNLWSI NKPNCIMSLT 

        70         80         90        100        110        120 
GHTSPVESVR LNTPEELIVA GSQSGSIRVW DLEAAKILRT LMGHKANICS LDFHPYGEFV 

       130        140        150        160        170        180 
ASGSQDTNIK LWDIRRKGCV FRYRGHSQAV RCLRFSPDGK WLASAADDHT VKLWDLTAGK 

       190        200        210        220        230        240 
MMSEFPGHTG PVNVVEFHPN EYLLASGSSD RTIRFWDLEK FQVVSCIEGE PGPVRSVLFN 

       250        260        270        280        290        300 
PDGCCLYSGC QDSLRVYGWE PERCFDVVLV NWGKVADLAI CNDQLIGVAF SQSNVSSYVV 

       310        320        330        340        350        360 
DLTRVTRTGT VARDPVQDHR PLAQPLPNPS APLRRIYERP STTCSKPQRV KQNSESERRS 

       370        380        390        400        410        420 
PSSEDDRDER ESRAEIQNAE DYNEIFQPKN SISRTPPRRS EPFPAPPEDD AATAKEAAKP 

       430        440        450        460        470        480 
SPAMDVQFPV PNLEVLPRPP VVASTPAPKA EPAIIPATRN EPIGLKASDF LPAVKIPQQA 

       490        500        510        520        530        540 
ELVDEDAMSQ IRKGHDTMCV VLTSRHKNLD TVRAVWTMGD IKTSVDSAVA INDLSVVVDL 

       550        560        570        580        590        600 
LNIVNQKASL WKLDLCTTVL PQIEKLLQSK YESYVQTGCT SLKLILQRFL PLITDMLAAP 

       610        620        630        640        650 
PSVGVDISRE ERLHKCRLCY KQLKSISGLV KSKSGLSGRH GSTFRELHLL MASLD 

« Hide

References

« Hide 'large scale' references
[1]"Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit."
Hartman J.J., Mahr J., McNally K., Okawa K., Iwamatsu A., Thomas S., Cheesman S., Heuser J., Vale R.D., McNally F.J.
Cell 93:277-287(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Muscle.
[7]"Katanin is responsible for the M-phase microtubule-severing activity in Xenopus eggs."
McNally F.J., Thomas S.
Mol. Biol. Cell 9:1847-1861(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin."
McNally K.P., Bazirgan O.A., McNally F.J.
J. Cell Sci. 113:1623-1633(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH KATNA1.
[9]"Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration."
Toyo-Oka K., Sasaki S., Yano Y., Mori D., Kobayashi T., Toyoshima Y.Y., Tokuoka S.M., Ishii S., Shimizu T., Muramatsu M., Hiraiwa N., Yoshiki A., Wynshaw-Boris A., Hirotsune S.
Hum. Mol. Genet. 14:3113-3128(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NDEL1, SUBCELLULAR LOCATION.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF052432 mRNA. Translation: AAC09328.1.
BT007022 mRNA. Translation: AAP35668.1.
CR456762 mRNA. Translation: CAG33043.1.
AC092118 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW82951.1.
BC001353 mRNA. Translation: AAH01353.1.
CCDSCCDS10788.1.
RefSeqNP_005877.2. NM_005886.2.
UniGeneHs.275675.
Hs.732486.

3D structure databases

ProteinModelPortalQ9BVA0.
SMRQ9BVA0. Positions 8-324.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115588. 15 interactions.
MINTMINT-4989412.
STRING9606.ENSP00000268509.

PTM databases

PhosphoSiteQ9BVA0.

Polymorphism databases

DMDM60390213.

Proteomic databases

MaxQBQ9BVA0.
PaxDbQ9BVA0.
PRIDEQ9BVA0.

Protocols and materials databases

DNASU10300.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000379661; ENSP00000368982; ENSG00000140854.
ENST00000594746; ENSP00000470131; ENSG00000268329.
GeneID10300.
KEGGhsa:10300.
UCSCuc002eml.1. human.

Organism-specific databases

CTD10300.
GeneCardsGC16P057770.
HGNCHGNC:6217. KATNB1.
HPAHPA041165.
HPA041839.
MIM602703. gene.
neXtProtNX_Q9BVA0.
PharmGKBPA30018.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2319.
HOGENOMHOG000008039.
HOVERGENHBG052294.
InParanoidQ9BVA0.
OMAACSKPQR.
PhylomeDBQ9BVA0.
TreeFamTF332359.

Enzyme and pathway databases

SignaLinkQ9BVA0.

Gene expression databases

ArrayExpressQ9BVA0.
BgeeQ9BVA0.
CleanExHS_KATNB1.
GenevestigatorQ9BVA0.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
HAMAPMF_03022. Katanin_p80_B1.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR026962. Katanin_bsu1.
IPR028021. Katanin_C-terminal.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF13925. Katanin_con80. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKATNB1.
GenomeRNAi10300.
NextBio39044.
PROQ9BVA0.
SOURCESearch...

Entry information

Entry nameKTNB1_HUMAN
AccessionPrimary (citable) accession number: Q9BVA0
Secondary accession number(s): A6NCG6, O60620
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM