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Protein

Katanin p80 WD40 repeat-containing subunit B1

Gene

KATNB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.UniRule annotation

GO - Molecular functioni

  • microtubule binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • metabolic process Source: GOC
  • microtubule severing Source: UniProtKB-HAMAP
  • mitotic chromosome movement towards spindle pole Source: UniProtKB-HAMAP
  • negative regulation of microtubule depolymerization Source: UniProtKB
  • positive regulation of cell death Source: Ensembl
  • positive regulation of microtubule depolymerization Source: UniProtKB
  • positive regulation of neuron projection development Source: Ensembl
  • protein targeting Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

SignaLinkiQ9BVA0.

Names & Taxonomyi

Protein namesi
Recommended name:
Katanin p80 WD40 repeat-containing subunit B1UniRule annotation
Short name:
Katanin p80 subunit B1UniRule annotation
Alternative name(s):
p80 kataninUniRule annotation
Gene namesi
Name:KATNB1UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:6217. KATNB1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: Ensembl
  • centrosome Source: UniProtKB-HAMAP
  • cytoplasm Source: UniProtKB
  • growth cone Source: Ensembl
  • katanin complex Source: UniProtKB
  • membrane Source: UniProtKB
  • microtubule Source: UniProtKB-KW
  • microtubule cytoskeleton Source: HPA
  • midbody Source: Ensembl
  • neuronal cell body Source: Ensembl
  • nucleus Source: UniProtKB
  • plasma membrane Source: HPA
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Involvement in diseasei

Lissencephaly 6, with microcephaly (LIS6)2 Publications

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA form of lissencephaly, a disorder of cortical development characterized by agyria or pachygyria and disorganization of the clear neuronal lamination of normal six-layered cortex. LIS6 features include hypoplasia of the corpus callosum, severe microcephaly and developmental delay.

See also OMIM:616212
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331G → W in LIS6. 1 Publication
VAR_073319
Natural varianti535 – 5351S → L in LIS6; causes reduced interaction with KATNA1 and NDEL1. 1 Publication
VAR_073320
Natural varianti540 – 5401L → R in LIS6. 1 Publication
VAR_073321

Keywords - Diseasei

Disease mutation, Lissencephaly

Organism-specific databases

MIMi616212. phenotype.
PharmGKBiPA30018.

Polymorphism and mutation databases

BioMutaiKATNB1.
DMDMi60390213.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655Katanin p80 WD40 repeat-containing subunit B1PRO_0000051049Add
BLAST

Proteomic databases

MaxQBiQ9BVA0.
PaxDbiQ9BVA0.
PRIDEiQ9BVA0.

PTM databases

PhosphoSiteiQ9BVA0.

Expressioni

Gene expression databases

BgeeiQ9BVA0.
CleanExiHS_KATNB1.
ExpressionAtlasiQ9BVA0. baseline and differential.
GenevisibleiQ9BVA0. HS.

Organism-specific databases

HPAiHPA041165.
HPA041839.

Interactioni

Subunit structurei

Interacts with PAFAH1B1 (By similarity). Interacts with KATNA1. This interaction enhances the microtubule binding and severing activity of KATNA1 and also targets this activity to the centrosome. Interacts with dynein, microtubules and NDEL1.By similarity2 Publications

Protein-protein interaction databases

BioGridi115588. 16 interactions.
MINTiMINT-4989412.
STRINGi9606.ENSP00000368982.

Structurei

3D structure databases

ProteinModelPortaliQ9BVA0.
SMRiQ9BVA0. Positions 8-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati18 – 5841WD 1Add
BLAST
Repeati61 – 10040WD 2Add
BLAST
Repeati103 – 14240WD 3Add
BLAST
Repeati145 – 18440WD 4Add
BLAST
Repeati187 – 22640WD 5Add
BLAST
Repeati229 – 26941WD 6Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 300300Interaction with centrosomesAdd
BLAST
Regioni1 – 284284Interaction with dyneinBy similarityAdd
BLAST
Regioni285 – 434150Interaction with PAFAH1B1By similarityAdd
BLAST
Regioni433 – 655223Interaction with KATNA1 and NDEL1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat KATNB1 family.UniRule annotation
Contains 6 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000118869.
HOGENOMiHOG000008039.
HOVERGENiHBG052294.
InParanoidiQ9BVA0.
KOiK18643.
OMAiDEEAMSQ.
PhylomeDBiQ9BVA0.
TreeFamiTF332359.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03022. Katanin_p80_B1.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR026962. Katanin_bsu1.
IPR028021. Katanin_C-terminal.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF13925. Katanin_con80. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BVA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPVVTKTA WKLQEIVAHA SNVSSLVLGK ASGRLLATGG DDCRVNLWSI
60 70 80 90 100
NKPNCIMSLT GHTSPVESVR LNTPEELIVA GSQSGSIRVW DLEAAKILRT
110 120 130 140 150
LMGHKANICS LDFHPYGEFV ASGSQDTNIK LWDIRRKGCV FRYRGHSQAV
160 170 180 190 200
RCLRFSPDGK WLASAADDHT VKLWDLTAGK MMSEFPGHTG PVNVVEFHPN
210 220 230 240 250
EYLLASGSSD RTIRFWDLEK FQVVSCIEGE PGPVRSVLFN PDGCCLYSGC
260 270 280 290 300
QDSLRVYGWE PERCFDVVLV NWGKVADLAI CNDQLIGVAF SQSNVSSYVV
310 320 330 340 350
DLTRVTRTGT VARDPVQDHR PLAQPLPNPS APLRRIYERP STTCSKPQRV
360 370 380 390 400
KQNSESERRS PSSEDDRDER ESRAEIQNAE DYNEIFQPKN SISRTPPRRS
410 420 430 440 450
EPFPAPPEDD AATAKEAAKP SPAMDVQFPV PNLEVLPRPP VVASTPAPKA
460 470 480 490 500
EPAIIPATRN EPIGLKASDF LPAVKIPQQA ELVDEDAMSQ IRKGHDTMCV
510 520 530 540 550
VLTSRHKNLD TVRAVWTMGD IKTSVDSAVA INDLSVVVDL LNIVNQKASL
560 570 580 590 600
WKLDLCTTVL PQIEKLLQSK YESYVQTGCT SLKLILQRFL PLITDMLAAP
610 620 630 640 650
PSVGVDISRE ERLHKCRLCY KQLKSISGLV KSKSGLSGRH GSTFRELHLL

MASLD
Length:655
Mass (Da):72,334
Last modified:June 1, 2001 - v1
Checksum:i411903E306B6EC16
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041H → L in AAC09328 (PubMed:9568719).Curated
Sequence conflicti211 – 2111R → G in AAC09328 (PubMed:9568719).Curated
Sequence conflicti226 – 2261C → R in AAC09328 (PubMed:9568719).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331G → W in LIS6. 1 Publication
VAR_073319
Natural varianti181 – 1811M → V.
Corresponds to variant rs60158050 [ dbSNP | Ensembl ].
VAR_062099
Natural varianti535 – 5351S → L in LIS6; causes reduced interaction with KATNA1 and NDEL1. 1 Publication
VAR_073320
Natural varianti540 – 5401L → R in LIS6. 1 Publication
VAR_073321

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF052432 mRNA. Translation: AAC09328.1.
BT007022 mRNA. Translation: AAP35668.1.
CR456762 mRNA. Translation: CAG33043.1.
AC092118 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW82951.1.
BC001353 mRNA. Translation: AAH01353.1.
CCDSiCCDS10788.1.
RefSeqiNP_005877.2. NM_005886.2.
UniGeneiHs.275675.
Hs.732486.

Genome annotation databases

EnsembliENST00000379661; ENSP00000368982; ENSG00000140854.
GeneIDi10300.
KEGGihsa:10300.
UCSCiuc002eml.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF052432 mRNA. Translation: AAC09328.1.
BT007022 mRNA. Translation: AAP35668.1.
CR456762 mRNA. Translation: CAG33043.1.
AC092118 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW82951.1.
BC001353 mRNA. Translation: AAH01353.1.
CCDSiCCDS10788.1.
RefSeqiNP_005877.2. NM_005886.2.
UniGeneiHs.275675.
Hs.732486.

3D structure databases

ProteinModelPortaliQ9BVA0.
SMRiQ9BVA0. Positions 8-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115588. 16 interactions.
MINTiMINT-4989412.
STRINGi9606.ENSP00000368982.

PTM databases

PhosphoSiteiQ9BVA0.

Polymorphism and mutation databases

BioMutaiKATNB1.
DMDMi60390213.

Proteomic databases

MaxQBiQ9BVA0.
PaxDbiQ9BVA0.
PRIDEiQ9BVA0.

Protocols and materials databases

DNASUi10300.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379661; ENSP00000368982; ENSG00000140854.
GeneIDi10300.
KEGGihsa:10300.
UCSCiuc002eml.1. human.

Organism-specific databases

CTDi10300.
GeneCardsiGC16P057770.
HGNCiHGNC:6217. KATNB1.
HPAiHPA041165.
HPA041839.
MIMi602703. gene.
616212. phenotype.
neXtProtiNX_Q9BVA0.
PharmGKBiPA30018.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00760000118869.
HOGENOMiHOG000008039.
HOVERGENiHBG052294.
InParanoidiQ9BVA0.
KOiK18643.
OMAiDEEAMSQ.
PhylomeDBiQ9BVA0.
TreeFamiTF332359.

Enzyme and pathway databases

SignaLinkiQ9BVA0.

Miscellaneous databases

GeneWikiiKATNB1.
GenomeRNAii10300.
NextBioi39044.
PROiQ9BVA0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BVA0.
CleanExiHS_KATNB1.
ExpressionAtlasiQ9BVA0. baseline and differential.
GenevisibleiQ9BVA0. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03022. Katanin_p80_B1.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR026962. Katanin_bsu1.
IPR028021. Katanin_C-terminal.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF13925. Katanin_con80. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit."
    Hartman J.J., Mahr J., McNally K., Okawa K., Iwamatsu A., Thomas S., Cheesman S., Heuser J., Vale R.D., McNally F.J.
    Cell 93:277-287(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Muscle.
  7. "Katanin is responsible for the M-phase microtubule-severing activity in Xenopus eggs."
    McNally F.J., Thomas S.
    Mol. Biol. Cell 9:1847-1861(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Two domains of p80 katanin regulate microtubule severing and spindle pole targeting by p60 katanin."
    McNally K.P., Bazirgan O.A., McNally F.J.
    J. Cell Sci. 113:1623-1633(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KATNA1.
  9. "Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration."
    Toyo-Oka K., Sasaki S., Yano Y., Mori D., Kobayashi T., Toyoshima Y.Y., Tokuoka S.M., Ishii S., Shimizu T., Muramatsu M., Hiraiwa N., Yoshiki A., Wynshaw-Boris A., Hirotsune S.
    Hum. Mol. Genet. 14:3113-3128(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NDEL1, SUBCELLULAR LOCATION.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: INVOLVEMENT IN LIS6, VARIANTS LIS6 LEU-535 AND ARG-540, CHARACTERIZATION OF VARIANT LIS6 LEU-535.
  12. Cited for: VARIANT LIS6 TRP-33.

Entry informationi

Entry nameiKTNB1_HUMAN
AccessioniPrimary (citable) accession number: Q9BVA0
Secondary accession number(s): A6NCG6, O60620
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: June 1, 2001
Last modified: July 22, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.