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Protein

Vesicle-associated membrane protein 8

Gene

VAMP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity).By similarity3 Publications

GO - Molecular functioni

  • chloride channel inhibitor activity Source: UniProtKB
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: GO_Central

GO - Biological processi

  • autophagosome maturation Source: UniProtKB
  • mucus secretion Source: UniProtKB
  • negative regulation of secretion by cell Source: UniProtKB
  • positive regulation of histamine secretion by mast cell Source: UniProtKB
  • post-Golgi vesicle-mediated transport Source: Reactome
  • protein transport Source: UniProtKB-KW
  • regulation of protein localization to plasma membrane Source: UniProtKB
  • vesicle fusion Source: GO_Central
  • viral entry into host cell Source: MGI
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-421837. Clathrin derived vesicle budding.
R-HSA-432720. Lysosome Vesicle Biogenesis.
R-HSA-432722. Golgi Associated Vesicle Biogenesis.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 81 Publication
Short name:
VAMP-81 Publication
Alternative name(s):
Endobrevin1 Publication
Short name:
EDB1 Publication
Gene namesi
Name:VAMP81 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:12647. VAMP8.

Subcellular locationi

  • Lysosome membrane By similarity; Single-pass type IV membrane protein Curated
  • Early endosome membrane 1 Publication; Single-pass type IV membrane protein Curated
  • Late endosome membrane By similarity; Single-pass type IV membrane protein Curated
  • Cell membrane By similarity; Single-pass type IV membrane protein Curated

  • Note: Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity). Sub-tight junctional domain in retinal pigment epithelium cells. Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell (By similarity). Cycles through the apical but not through the basolateral plasma membrane (By similarity). Apical region of acinar cells; in zymogen granule membranes (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7575CytoplasmicSequence analysisAdd
BLAST
Transmembranei76 – 9621Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini97 – 1004VesicularSequence analysis

GO - Cellular componenti

  • azurophil granule membrane Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB
  • early endosome Source: ProtInc
  • early endosome membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • late endosome membrane Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • mucin granule Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • recycling endosome Source: UniProtKB
  • secretory granule membrane Source: UniProtKB
  • SNARE complex Source: UniProtKB
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37271.

Polymorphism and mutation databases

BioMutaiVAMP8.
DMDMi55976764.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 100100Vesicle-associated membrane protein 8PRO_0000206736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources
Modified residuei5 – 51PhosphoserineCombined sources
Modified residuei18 – 181PhosphoserineCombined sources
Modified residuei28 – 281PhosphothreonineCombined sources
Modified residuei48 – 481PhosphothreonineCombined sources
Modified residuei54 – 541PhosphothreonineCombined sources
Modified residuei55 – 551PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BV40.
MaxQBiQ9BV40.
PaxDbiQ9BV40.
PeptideAtlasiQ9BV40.
PRIDEiQ9BV40.
TopDownProteomicsiQ9BV40.

PTM databases

iPTMnetiQ9BV40.
PhosphoSiteiQ9BV40.

Expressioni

Tissue specificityi

Platelets.1 Publication

Gene expression databases

BgeeiENSG00000118640.
CleanExiHS_VAMP8.
ExpressionAtlasiQ9BV40. baseline and differential.
GenevisibleiQ9BV40. HS.

Organism-specific databases

HPAiCAB037132.
CAB037147.
HPA006882.

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (PubMed:25686604). Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:12130530).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Interaction with STX8By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
STX17P569628EBI-727028,EBI-2797775

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114221. 37 interactions.
DIPiDIP-40358N.
IntActiQ9BV40. 18 interactions.
MINTiMINT-4649115.
STRINGi9606.ENSP00000263864.

Structurei

Secondary structure

1
100
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 7261Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WY4X-ray1.40A11-74[»]
ProteinModelPortaliQ9BV40.
SMRiQ9BV40. Positions 11-74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 7261v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ9BV40.
KOiK08512.
OMAiKMIVIIC.
OrthoDBiEOG091G0MPE.
PhylomeDBiQ9BV40.
TreeFamiTF320419.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BV40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEASEGGGN DRVRNLQSEV EGVKNIMTQN VERILARGEN LEHLRNKTED
60 70 80 90 100
LEATSEHFKT TSQKVARKFW WKNVKMIVLI CVIVFIIILF IVLFATGAFS
Length:100
Mass (Da):11,438
Last modified:June 1, 2001 - v1
Checksum:i018B986442BBD9B6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121R → L in AAC08434 (PubMed:9614193).Curated
Sequence conflicti81 – 811C → R in CAG33276 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053233 mRNA. Translation: AAC08434.1.
BT006700 mRNA. Translation: AAP35346.1.
CR456995 mRNA. Translation: CAG33276.1.
AC016753 Genomic DNA. Translation: AAY24341.1.
BC001634 mRNA. Translation: AAH01634.1.
CCDSiCCDS1979.1.
RefSeqiNP_003752.2. NM_003761.4.
UniGeneiHs.714302.

Genome annotation databases

EnsembliENST00000263864; ENSP00000263864; ENSG00000118640.
GeneIDi8673.
KEGGihsa:8673.
UCSCiuc002spt.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053233 mRNA. Translation: AAC08434.1.
BT006700 mRNA. Translation: AAP35346.1.
CR456995 mRNA. Translation: CAG33276.1.
AC016753 Genomic DNA. Translation: AAY24341.1.
BC001634 mRNA. Translation: AAH01634.1.
CCDSiCCDS1979.1.
RefSeqiNP_003752.2. NM_003761.4.
UniGeneiHs.714302.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4WY4X-ray1.40A11-74[»]
ProteinModelPortaliQ9BV40.
SMRiQ9BV40. Positions 11-74.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114221. 37 interactions.
DIPiDIP-40358N.
IntActiQ9BV40. 18 interactions.
MINTiMINT-4649115.
STRINGi9606.ENSP00000263864.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

iPTMnetiQ9BV40.
PhosphoSiteiQ9BV40.

Polymorphism and mutation databases

BioMutaiVAMP8.
DMDMi55976764.

Proteomic databases

EPDiQ9BV40.
MaxQBiQ9BV40.
PaxDbiQ9BV40.
PeptideAtlasiQ9BV40.
PRIDEiQ9BV40.
TopDownProteomicsiQ9BV40.

Protocols and materials databases

DNASUi8673.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263864; ENSP00000263864; ENSG00000118640.
GeneIDi8673.
KEGGihsa:8673.
UCSCiuc002spt.5. human.

Organism-specific databases

CTDi8673.
GeneCardsiVAMP8.
HGNCiHGNC:12647. VAMP8.
HPAiCAB037132.
CAB037147.
HPA006882.
MIMi603177. gene.
neXtProtiNX_Q9BV40.
PharmGKBiPA37271.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ9BV40.
KOiK08512.
OMAiKMIVIIC.
OrthoDBiEOG091G0MPE.
PhylomeDBiQ9BV40.
TreeFamiTF320419.

Enzyme and pathway databases

ReactomeiR-HSA-421837. Clathrin derived vesicle budding.
R-HSA-432720. Lysosome Vesicle Biogenesis.
R-HSA-432722. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

GeneWikiiVesicle-associated_membrane_protein_8.
GenomeRNAii8673.
PROiQ9BV40.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000118640.
CleanExiHS_VAMP8.
ExpressionAtlasiQ9BV40. baseline and differential.
GenevisibleiQ9BV40. HS.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP8_HUMAN
AccessioniPrimary (citable) accession number: Q9BV40
Secondary accession number(s): O60625, Q53SP9, Q6IB09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.