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Protein

Vesicle-associated membrane protein 8

Gene

VAMP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity).By similarity3 Publications

GO - Molecular functioni

  • chloride channel inhibitor activity Source: UniProtKB
  • SNAP receptor activity Source: GO_Central
  • SNARE binding Source: GO_Central

GO - Biological processi

  • antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: Reactome
  • autophagosome maturation Source: UniProtKB
  • autophagosome membrane docking Source: GO_Central
  • membrane organization Source: Reactome
  • mucus secretion Source: UniProtKB
  • negative regulation of secretion by cell Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • positive regulation of histamine secretion by mast cell Source: UniProtKB
  • post-Golgi vesicle-mediated transport Source: Reactome
  • protein transport Source: UniProtKB-KW
  • regulation of protein localization to plasma membrane Source: UniProtKB
  • vesicle fusion Source: GO_Central
  • viral entry into host cell Source: MGI

Keywordsi

Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1236974. ER-Phagosome pathway.
R-HSA-421837. Clathrin derived vesicle budding.
R-HSA-432720. Lysosome Vesicle Biogenesis.
R-HSA-432722. Golgi Associated Vesicle Biogenesis.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-HSA-8856828. Clathrin-mediated endocytosis.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 81 Publication
Short name:
VAMP-81 Publication
Alternative name(s):
Endobrevin1 Publication
Short name:
EDB1 Publication
Gene namesi
Name:VAMP81 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000118640.10.
HGNCiHGNC:12647. VAMP8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 75CytoplasmicSequence analysisAdd BLAST75
Transmembranei76 – 96Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini97 – 100VesicularSequence analysis4

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8673.
OpenTargetsiENSG00000118640.
PharmGKBiPA37271.

Polymorphism and mutation databases

BioMutaiVAMP8.
DMDMi55976764.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067361 – 100Vesicle-associated membrane protein 8Add BLAST100

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei18PhosphoserineCombined sources1
Modified residuei28PhosphothreonineCombined sources1
Modified residuei48PhosphothreonineCombined sources1
Modified residuei54PhosphothreonineCombined sources1
Modified residuei55PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BV40.
MaxQBiQ9BV40.
PaxDbiQ9BV40.
PeptideAtlasiQ9BV40.
PRIDEiQ9BV40.
TopDownProteomicsiQ9BV40.

PTM databases

iPTMnetiQ9BV40.
PhosphoSitePlusiQ9BV40.

Expressioni

Tissue specificityi

Platelets.1 Publication

Gene expression databases

BgeeiENSG00000118640.
CleanExiHS_VAMP8.
ExpressionAtlasiQ9BV40. baseline and differential.
GenevisibleiQ9BV40. HS.

Organism-specific databases

HPAiCAB037132.
CAB037147.
HPA006882.

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (PubMed:25686604). Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:12130530).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Interaction with STX8By similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
STX17P569628EBI-727028,EBI-2797775

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114221. 40 interactors.
CORUMiQ9BV40.
DIPiDIP-40358N.
IntActiQ9BV40. 18 interactors.
MINTiMINT-4649115.
STRINGi9606.ENSP00000263864.

Structurei

Secondary structure

1100
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 72Combined sources61

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WY4X-ray1.40A11-74[»]
ProteinModelPortaliQ9BV40.
SMRiQ9BV40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 72v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ9BV40.
KOiK08512.
OMAiKMIVIIC.
OrthoDBiEOG091G0MPE.
PhylomeDBiQ9BV40.
TreeFamiTF320419.

Family and domain databases

InterProiView protein in InterPro
IPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
PfamiView protein in Pfam
PF00957. Synaptobrevin. 1 hit.
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiView protein in PROSITE
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BV40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEASEGGGN DRVRNLQSEV EGVKNIMTQN VERILARGEN LEHLRNKTED
60 70 80 90 100
LEATSEHFKT TSQKVARKFW WKNVKMIVLI CVIVFIIILF IVLFATGAFS
Length:100
Mass (Da):11,438
Last modified:June 1, 2001 - v1
Checksum:i018B986442BBD9B6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12R → L in AAC08434 (PubMed:9614193).Curated1
Sequence conflicti81C → R in CAG33276 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053233 mRNA. Translation: AAC08434.1.
BT006700 mRNA. Translation: AAP35346.1.
CR456995 mRNA. Translation: CAG33276.1.
AC016753 Genomic DNA. Translation: AAY24341.1.
BC001634 mRNA. Translation: AAH01634.1.
CCDSiCCDS1979.1.
RefSeqiNP_003752.2. NM_003761.4.
UniGeneiHs.714302.

Genome annotation databases

EnsembliENST00000263864; ENSP00000263864; ENSG00000118640.
GeneIDi8673.
KEGGihsa:8673.
UCSCiuc002spt.5. human.

Similar proteinsi

Entry informationi

Entry nameiVAMP8_HUMAN
AccessioniPrimary (citable) accession number: Q9BV40
Secondary accession number(s): O60625, Q53SP9, Q6IB09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: June 1, 2001
Last modified: September 27, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families