Q9BV40 (VAMP8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle-associated membrane protein 8
Short name=VAMP-8

Alternative name(s):
Endobrevin
Short name=EDB

Gene names

Name:

VAMP8

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	100 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes By similarity. Ref.6
Subunit structure	Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B By similarity. Interacts with STX8 By similarity.
Subcellular location	Membrane; Single-pass type IV membrane protein Probable Ref.1.
Tissue specificity	Platelets.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	post-Golgi vesicle-mediated transport Traceable author statement. Source: Reactome
Cellular component	early endosome Traceable author statement. Source: ProtInc integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane fraction Traceable author statement. Source: ProtInc plasma membrane Traceable author statement. Source: Reactome secretory granule membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 100	100	Vesicle-associated membrane protein 8		PRO_0000206736
Regions
	Topological domain	1 – 75	75	Cytoplasmic Potential
	Transmembrane	76 – 96	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	97 – 100	4	Vesicular Potential
	Domain	12 – 72	61	v-SNARE coiled-coil homology
Sites
	Site	33	1	Interaction with STX8 By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.7
	Modified residue	18	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	12	1	R → L in AAC08434. Ref.1
	Sequence conflict	81	1	C → R in CAG33276. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9BV40 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 018B986442BBD9B6	FASTA	100	11,438
	10 20 30 40 50 60 MEEASEGGGN DRVRNLQSEV EGVKNIMTQN VERILARGEN LEHLRNKTED LEATSEHFKT 70 80 90 100 TSQKVARKFW WKNVKMIVLI CVIVFIIILF IVLFATGAFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially associated with the early endosome." Wong S.H., Zhang T., Xu Y., Subramaniam V.N., Griffiths G., Hong W. Mol. Biol. Cell 9:1549-1563(1998) [PubMed: 9614193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	"Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion." Polgar J., Chung S.H., Reed G.L. Blood 100:1081-1083(2002) [PubMed: 12130530] [Abstract] Cited for: PROTEIN SEQUENCE OF 15-24 AND 38-59, FUNCTION IN SECRETION, IDENTIFICATION IN A COMPLEX WITH STX1A AND SNAP23.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF053233 mRNA. Translation: AAC08434.1. BT006700 mRNA. Translation: AAP35346.1. CR456995 mRNA. Translation: CAG33276.1. AC016753 Genomic DNA. Translation: AAY24341.1. BC001634 mRNA. Translation: AAH01634.1.
IPI	IPI00030911.
RefSeq	NP_003752.2. NM_003761.4.
UniGene	Hs.714302.
3D structure databases
ProteinModelPortal	Q9BV40.
SMR	Q9BV40. Positions 12-64.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BV40. 6 interactions.
MINT	MINT-4649115.
STRING	Q9BV40.
Protein family/group databases
TCDB	1.F.1.1.1. synaptosomal vesicle fusion pore (SVF-Pore) family.
PTM databases
PhosphoSite	Q9BV40.
Polymorphism databases
DMDM	55976764.
Proteomic databases
PeptideAtlas	Q9BV40.
PRIDE	Q9BV40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263864; ENSP00000263864; ENSG00000118640.
GeneID	8673.
KEGG	hsa:8673.
UCSC	uc002spt.2. human.
Organism-specific databases
CTD	8673.
GeneCards	GC02P085788.
H-InvDB	HIX0002222.
HGNC	HGNC:12647. VAMP8.
HPA	HPA006882.
MIM	603177. gene.
neXtProt	NX_Q9BV40.
PharmGKB	PA37271.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG21029.
GeneTree	ENSGT00550000074449.
HOGENOM	HBG386095.
HOVERGEN	HBG006675.
InParanoid	Q9BV40.
OMA	KMIVIIC.
OrthoDB	EOG4FBHVK.
PhylomeDB	Q9BV40.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking.
Gene expression databases
ArrayExpress	Q9BV40.
Bgee	Q9BV40.
CleanEx	HS_VAMP8.
Genevestigator	Q9BV40.
GermOnline	ENSG00000118640. Homo sapiens.
Family and domain databases
InterPro	IPR001388. Synaptobrevin. IPR016444. Synaptobrevin_met/fun. [Graphical view]
KO	K08512.
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PIRSF	PIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTS	PR00219. SYNAPTOBREVN.
PROSITE	PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	32533.
SOURCE	Search...

Entry information

Entry name

VAMP8_HUMAN

Accession

Primary (citable) accession number: Q9BV40
Secondary accession number(s): O60625, Q53SP9, Q6IB09

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.