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Protein

Methylthioribose-1-phosphate isomerase

Gene

MRI1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P). Independently from catalytic activity, promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation and stress fiber turnover.UniRule annotation1 Publication

Catalytic activityi

S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (MRI1)
  2. Methylthioribulose-1-phosphate dehydratase (APIP), Methylthioribulose-1-phosphate dehydratase (APIP)
  3. Enolase-phosphatase E1 (ENOPH1)
  4. Enolase-phosphatase E1 (ENOPH1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei168Transition state stabilizer1
Active sitei248Proton donorUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

BioCyciZFISH:HS00525-MONOMER.
BRENDAi5.3.1.23. 2681.
ReactomeiR-HSA-1237112. Methionine salvage pathway.
UniPathwayiUPA00904; UER00874.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylthioribose-1-phosphate isomeraseUniRule annotation (EC:5.3.1.23UniRule annotation)
Short name:
M1PiUniRule annotation
Short name:
MTR-1-P isomeraseUniRule annotation
Alternative name(s):
Mediator of RhoA-dependent invasion
S-methyl-5-thioribose-1-phosphate isomeraseUniRule annotation
Translation initiation factor eIF-2B subunit alpha/beta/delta-like proteinUniRule annotation
Gene namesi
Name:MRI1UniRule annotation
Synonyms:MRDI
ORF Names:UNQ6390/PRO21135
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:28469. MRI1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168C → S: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi248D → A: Abolishes enzymatic activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000037757.
PharmGKBiPA164723110.

Polymorphism and mutation databases

BioMutaiMRI1.
DMDMi74733279.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003173251 – 369Methylthioribose-1-phosphate isomeraseAdd BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9BV20.
MaxQBiQ9BV20.
PaxDbiQ9BV20.
PeptideAtlasiQ9BV20.
PRIDEiQ9BV20.

PTM databases

iPTMnetiQ9BV20.
PhosphoSitePlusiQ9BV20.

Expressioni

Inductioni

By RhoA activation in cancer cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000037757.
GenevisibleiQ9BV20. HS.

Organism-specific databases

HPAiCAB045988.
HPA042744.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-747381,EBI-747381
PICK1Q9NRD54EBI-747381,EBI-79165

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi123973. 8 interactors.
IntActiQ9BV20. 8 interactors.
MINTiMINT-1197605.
STRINGi9606.ENSP00000040663.

Structurei

Secondary structure

1369
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Beta strandi12 – 16Combined sources5
Helixi18 – 20Combined sources3
Turni21 – 23Combined sources3
Beta strandi27 – 30Combined sources4
Helixi33 – 41Combined sources9
Helixi48 – 63Combined sources16
Turni64 – 66Combined sources3
Beta strandi70 – 72Combined sources3
Helixi73 – 87Combined sources15
Helixi95 – 112Combined sources18
Helixi118 – 155Combined sources38
Beta strandi162 – 166Combined sources5
Turni172 – 174Combined sources3
Beta strandi175 – 178Combined sources4
Helixi181 – 190Combined sources10
Beta strandi194 – 200Combined sources7
Turni203 – 206Combined sources4
Helixi207 – 210Combined sources4
Helixi212 – 218Combined sources7
Beta strandi223 – 226Combined sources4
Helixi228 – 230Combined sources3
Helixi231 – 237Combined sources7
Beta strandi242 – 245Combined sources4
Beta strandi248 – 251Combined sources4
Beta strandi256 – 259Combined sources4
Helixi262 – 271Combined sources10
Beta strandi276 – 279Combined sources4
Helixi282 – 284Combined sources3
Helixi292 – 294Combined sources3
Helixi303 – 306Combined sources4
Beta strandi323 – 328Combined sources6
Helixi330 – 332Combined sources3
Beta strandi335 – 339Combined sources5
Beta strandi342 – 344Combined sources3
Helixi346 – 348Combined sources3
Helixi349 – 353Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LDQX-ray2.50A/B1-369[»]
4LDRX-ray2.29A/B1-369[»]
ProteinModelPortaliQ9BV20.
SMRiQ9BV20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG1468. Eukaryota.
COG0182. LUCA.
GeneTreeiENSGT00390000013732.
HOGENOMiHOG000224730.
InParanoidiQ9BV20.
KOiK08963.
OMAiAIPGGDH.
OrthoDBiEOG091G0BA6.
PhylomeDBiQ9BV20.
TreeFamiTF300852.

Family and domain databases

Gene3Di1.20.120.420. 1 hit.
HAMAPiMF_01678. Salvage_MtnA. 1 hit.
InterProiIPR000649. IF-2B-related.
IPR005251. IF-M1Pi.
IPR011559. Initiation_fac_2B_a/b/d.
IPR027363. M1Pi_N.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00524. eIF-2B_rel. 1 hit.
TIGR00512. salvage_mtnA. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BV20-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLEAIRYSR GSLQILDQLL LPKQSRYEAV GSVHQAWEAI RAMKVRGAPA
60 70 80 90 100
IALVGCLSLA VELQAGAGGP GLAALVAFVR DKLSFLVTAR PTAVNMARAA
110 120 130 140 150
RDLADVAARE AEREGATEEA VRERVICCTE DMLEKDLRDN RSIGDLGARH
160 170 180 190 200
LLERVAPSGG KVTVLTHCNT GALATAGYGT ALGVIRSLHS LGRLEHAFCT
210 220 230 240 250
ETRPYNQGAR LTAFELVYEQ IPATLITDSM VAAAMAHRGV SAVVVGADRV
260 270 280 290 300
VANGDTANKV GTYQLAIVAK HHGIPFYVAA PSSSCDLRLE TGKEIIIEER
310 320 330 340 350
PGQELTDVNG VRIAAPGIGV WNPAFDVTPH DLITGGIITE LGVFAPEELR
360
TALTTTISSR DGTLDGPQM
Length:369
Mass (Da):39,150
Last modified:June 1, 2001 - v1
Checksum:iA6A1B5EC76632F4F
GO
Isoform 2 (identifier: Q9BV20-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     125-200: VICCTEDMLE...LGRLEHAFCT → RETELCEHWEEHTRQRELPLRGPLGGTVL

Show »
Length:322
Mass (Da):34,529
Checksum:iB9C6A0C26615AA76
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059253235M → V.Corresponds to variant rs35098252dbSNPEnsembl.1
Natural variantiVAR_059254319G → A.Corresponds to variant rs10402855dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030935125 – 200VICCT…HAFCT → RETELCEHWEEHTRQRELPL RGPLGGTVL in isoform 2. 1 PublicationAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358176 mRNA. Translation: AAQ88543.1.
AL834276 mRNA. Translation: CAD38951.1.
CH471106 Genomic DNA. Translation: EAW84370.1.
CH471106 Genomic DNA. Translation: EAW84371.1.
BC001703 mRNA. Translation: AAH01703.1.
CCDSiCCDS12297.1. [Q9BV20-2]
CCDS32923.1. [Q9BV20-1]
RefSeqiNP_001026897.1. NM_001031727.3. [Q9BV20-1]
NP_001316501.1. NM_001329572.1.
NP_115661.1. NM_032285.3. [Q9BV20-2]
UniGeneiHs.439370.

Genome annotation databases

EnsembliENST00000040663; ENSP00000040663; ENSG00000037757. [Q9BV20-1]
ENST00000319545; ENSP00000314871; ENSG00000037757. [Q9BV20-2]
GeneIDi84245.
KEGGihsa:84245.
UCSCiuc002mxe.4. human. [Q9BV20-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358176 mRNA. Translation: AAQ88543.1.
AL834276 mRNA. Translation: CAD38951.1.
CH471106 Genomic DNA. Translation: EAW84370.1.
CH471106 Genomic DNA. Translation: EAW84371.1.
BC001703 mRNA. Translation: AAH01703.1.
CCDSiCCDS12297.1. [Q9BV20-2]
CCDS32923.1. [Q9BV20-1]
RefSeqiNP_001026897.1. NM_001031727.3. [Q9BV20-1]
NP_001316501.1. NM_001329572.1.
NP_115661.1. NM_032285.3. [Q9BV20-2]
UniGeneiHs.439370.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LDQX-ray2.50A/B1-369[»]
4LDRX-ray2.29A/B1-369[»]
ProteinModelPortaliQ9BV20.
SMRiQ9BV20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123973. 8 interactors.
IntActiQ9BV20. 8 interactors.
MINTiMINT-1197605.
STRINGi9606.ENSP00000040663.

PTM databases

iPTMnetiQ9BV20.
PhosphoSitePlusiQ9BV20.

Polymorphism and mutation databases

BioMutaiMRI1.
DMDMi74733279.

Proteomic databases

EPDiQ9BV20.
MaxQBiQ9BV20.
PaxDbiQ9BV20.
PeptideAtlasiQ9BV20.
PRIDEiQ9BV20.

Protocols and materials databases

DNASUi84245.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000040663; ENSP00000040663; ENSG00000037757. [Q9BV20-1]
ENST00000319545; ENSP00000314871; ENSG00000037757. [Q9BV20-2]
GeneIDi84245.
KEGGihsa:84245.
UCSCiuc002mxe.4. human. [Q9BV20-1]

Organism-specific databases

CTDi84245.
GeneCardsiMRI1.
HGNCiHGNC:28469. MRI1.
HPAiCAB045988.
HPA042744.
MIMi615105. gene.
neXtProtiNX_Q9BV20.
OpenTargetsiENSG00000037757.
PharmGKBiPA164723110.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1468. Eukaryota.
COG0182. LUCA.
GeneTreeiENSGT00390000013732.
HOGENOMiHOG000224730.
InParanoidiQ9BV20.
KOiK08963.
OMAiAIPGGDH.
OrthoDBiEOG091G0BA6.
PhylomeDBiQ9BV20.
TreeFamiTF300852.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00874.
BioCyciZFISH:HS00525-MONOMER.
BRENDAi5.3.1.23. 2681.
ReactomeiR-HSA-1237112. Methionine salvage pathway.

Miscellaneous databases

GeneWikiiMGC3207.
GenomeRNAii84245.
PROiQ9BV20.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000037757.
GenevisibleiQ9BV20. HS.

Family and domain databases

Gene3Di1.20.120.420. 1 hit.
HAMAPiMF_01678. Salvage_MtnA. 1 hit.
InterProiIPR000649. IF-2B-related.
IPR005251. IF-M1Pi.
IPR011559. Initiation_fac_2B_a/b/d.
IPR027363. M1Pi_N.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00524. eIF-2B_rel. 1 hit.
TIGR00512. salvage_mtnA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTNA_HUMAN
AccessioniPrimary (citable) accession number: Q9BV20
Secondary accession number(s): Q8NDC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.