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Protein

Methylthioribose-1-phosphate isomerase

Gene

MRI1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P). Independently from catalytic activity, promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation and stress fiber turnover.UniRule annotation1 Publication

Catalytic activityi

S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (MRI1)
  2. Methylthioribulose-1-phosphate dehydratase (APIP), Methylthioribulose-1-phosphate dehydratase (APIP)
  3. Enolase-phosphatase E1 (ENOPH1)
  4. Enolase-phosphatase E1 (ENOPH1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei168Transition state stabilizer1
Active sitei248Proton donorUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Methionine biosynthesis

Enzyme and pathway databases

BRENDAi5.3.1.23 2681
ReactomeiR-HSA-1237112 Methionine salvage pathway
UniPathwayiUPA00904; UER00874

Names & Taxonomyi

Protein namesi
Recommended name:
Methylthioribose-1-phosphate isomeraseUniRule annotation (EC:5.3.1.23UniRule annotation)
Short name:
M1PiUniRule annotation
Short name:
MTR-1-P isomeraseUniRule annotation
Alternative name(s):
Mediator of RhoA-dependent invasion
S-methyl-5-thioribose-1-phosphate isomeraseUniRule annotation
Translation initiation factor eIF-2B subunit alpha/beta/delta-like proteinUniRule annotation
Gene namesi
Name:MRI1UniRule annotation
Synonyms:MRDI
ORF Names:UNQ6390/PRO21135
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000037757.13
HGNCiHGNC:28469 MRI1
MIMi615105 gene
neXtProtiNX_Q9BV20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168C → S: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi248D → A: Abolishes enzymatic activity. 1 Publication1

Organism-specific databases

DisGeNETi84245
OpenTargetsiENSG00000037757
PharmGKBiPA164723110

Polymorphism and mutation databases

BioMutaiMRI1
DMDMi74733279

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003173251 – 369Methylthioribose-1-phosphate isomeraseAdd BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9BV20
MaxQBiQ9BV20
PaxDbiQ9BV20
PeptideAtlasiQ9BV20
PRIDEiQ9BV20

PTM databases

iPTMnetiQ9BV20
PhosphoSitePlusiQ9BV20

Expressioni

Inductioni

By RhoA activation in cancer cells (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000037757
GenevisibleiQ9BV20 HS

Organism-specific databases

HPAiCAB045988
HPA042744

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi1239739 interactors.
IntActiQ9BV20 10 interactors.
MINTiQ9BV20
STRINGi9606.ENSP00000040663

Structurei

Secondary structure

1369
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Beta strandi12 – 16Combined sources5
Helixi18 – 20Combined sources3
Turni21 – 23Combined sources3
Beta strandi27 – 30Combined sources4
Helixi33 – 41Combined sources9
Helixi48 – 63Combined sources16
Turni64 – 66Combined sources3
Beta strandi70 – 72Combined sources3
Helixi73 – 87Combined sources15
Helixi95 – 112Combined sources18
Helixi118 – 155Combined sources38
Beta strandi162 – 166Combined sources5
Turni172 – 174Combined sources3
Beta strandi175 – 178Combined sources4
Helixi181 – 190Combined sources10
Beta strandi194 – 200Combined sources7
Turni203 – 206Combined sources4
Helixi207 – 210Combined sources4
Helixi212 – 218Combined sources7
Beta strandi223 – 226Combined sources4
Helixi228 – 230Combined sources3
Helixi231 – 237Combined sources7
Beta strandi242 – 245Combined sources4
Beta strandi248 – 251Combined sources4
Beta strandi256 – 259Combined sources4
Helixi262 – 271Combined sources10
Beta strandi276 – 279Combined sources4
Helixi282 – 284Combined sources3
Helixi292 – 294Combined sources3
Helixi303 – 306Combined sources4
Beta strandi323 – 328Combined sources6
Helixi330 – 332Combined sources3
Beta strandi335 – 339Combined sources5
Beta strandi342 – 344Combined sources3
Helixi346 – 348Combined sources3
Helixi349 – 353Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4LDQX-ray2.50A/B1-369[»]
4LDRX-ray2.29A/B1-369[»]
ProteinModelPortaliQ9BV20
SMRiQ9BV20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG1468 Eukaryota
COG0182 LUCA
GeneTreeiENSGT00390000013732
HOGENOMiHOG000224730
InParanoidiQ9BV20
KOiK08963
OMAiVNVLTHC
OrthoDBiEOG091G0BA6
PhylomeDBiQ9BV20
TreeFamiTF300852

Family and domain databases

Gene3Di1.20.120.4201 hit
HAMAPiMF_01678 Salvage_MtnA, 1 hit
InterProiView protein in InterPro
IPR000649 IF-2B-related
IPR005251 IF-M1Pi
IPR011559 Initiation_fac_2B_a/b/d
IPR027363 M1Pi_N
IPR037171 NagB/RpiA_transferase-like
PfamiView protein in Pfam
PF01008 IF-2B, 1 hit
SUPFAMiSSF100950 SSF100950, 1 hit
TIGRFAMsiTIGR00524 eIF-2B_rel, 1 hit
TIGR00512 salvage_mtnA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BV20-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLEAIRYSR GSLQILDQLL LPKQSRYEAV GSVHQAWEAI RAMKVRGAPA
60 70 80 90 100
IALVGCLSLA VELQAGAGGP GLAALVAFVR DKLSFLVTAR PTAVNMARAA
110 120 130 140 150
RDLADVAARE AEREGATEEA VRERVICCTE DMLEKDLRDN RSIGDLGARH
160 170 180 190 200
LLERVAPSGG KVTVLTHCNT GALATAGYGT ALGVIRSLHS LGRLEHAFCT
210 220 230 240 250
ETRPYNQGAR LTAFELVYEQ IPATLITDSM VAAAMAHRGV SAVVVGADRV
260 270 280 290 300
VANGDTANKV GTYQLAIVAK HHGIPFYVAA PSSSCDLRLE TGKEIIIEER
310 320 330 340 350
PGQELTDVNG VRIAAPGIGV WNPAFDVTPH DLITGGIITE LGVFAPEELR
360
TALTTTISSR DGTLDGPQM
Length:369
Mass (Da):39,150
Last modified:June 1, 2001 - v1
Checksum:iA6A1B5EC76632F4F
GO
Isoform 2 (identifier: Q9BV20-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     125-200: VICCTEDMLE...LGRLEHAFCT → RETELCEHWEEHTRQRELPLRGPLGGTVL

Show »
Length:322
Mass (Da):34,529
Checksum:iB9C6A0C26615AA76
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059253235M → V. Corresponds to variant dbSNP:rs35098252Ensembl.1
Natural variantiVAR_059254319G → A. Corresponds to variant dbSNP:rs10402855Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030935125 – 200VICCT…HAFCT → RETELCEHWEEHTRQRELPL RGPLGGTVL in isoform 2. 1 PublicationAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358176 mRNA Translation: AAQ88543.1
AL834276 mRNA Translation: CAD38951.1
CH471106 Genomic DNA Translation: EAW84370.1
CH471106 Genomic DNA Translation: EAW84371.1
BC001703 mRNA Translation: AAH01703.1
CCDSiCCDS12297.1 [Q9BV20-2]
CCDS32923.1 [Q9BV20-1]
RefSeqiNP_001026897.1, NM_001031727.3 [Q9BV20-1]
NP_001316501.1, NM_001329572.1
NP_115661.1, NM_032285.3 [Q9BV20-2]
UniGeneiHs.439370

Genome annotation databases

EnsembliENST00000040663; ENSP00000040663; ENSG00000037757 [Q9BV20-1]
ENST00000319545; ENSP00000314871; ENSG00000037757 [Q9BV20-2]
GeneIDi84245
KEGGihsa:84245
UCSCiuc002mxe.4 human [Q9BV20-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiMTNA_HUMAN
AccessioniPrimary (citable) accession number: Q9BV20
Secondary accession number(s): Q8NDC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome