Reviewed, UniProtKB/Swiss-Prot Q9BUQ8 (DDX23_HUMAN)

Names and origin

Protein names

Recommended name:
    Probable ATP-dependent RNA helicase DDX23
    EC=3.6.1.-
Alternative name(s):
    DEAD box protein 23
    100 kDa U5 snRNP-specific protein
    U5-100kD
    PRP28 homolog

Gene names

Name:

DDX23

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	820 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably involved in pre-mRNA splicing.
Subunit structure	Component of the U5 and U5.4/6 snRNP complexes. Identified in the spliceosome C complex, at least composed of AQR, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRNP200, SNRNP40, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, XAB2 and ZCCHC8.
Subcellular location	Nucleus. Ref.1
Post-translational modification	In vitro phosphorylated by CLK1 and U1 snRNP-associated protein kinase. Ref.1 Ref.4 Ref.5
Sequence similarities	Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cis assembly of pre-catalytic spliceosome Inferred by curator. Source: HGNC
Cellular component	snRNP U5 Inferred from direct assay. Source: HGNC
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent RNA helicase activity Ref.1 Traceable author statement. Source: ProtInc RNA splicing factor activity, transesterification mechanism Ref.1 Traceable author statement. Source: UniProtKB nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CD2BP2	O95400	1	EBI-540096,EBI-768015
RNPS1	Q15287	1	EBI-540096,EBI-395959
SNRNP40	Q96DI7	1	EBI-540096,EBI-538492

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 820	820	Probable ATP-dependent RNA helicase DDX23		PRO_0000055128
Regions
	Domain	422 – 627	206	Helicase ATP-binding
	Domain	651 – 799	149	Helicase C-terminal
	Nucleotide binding	435 – 442	8	ATP By similarity
	Motif	391 – 419	29	Q motif
	Motif	549 – 552	4	DEAD box
	Compositional bias	20 – 122	103	Arg-rich
	Compositional bias	128 – 240	113	Glu-rich
Amino acid modifications
	Modified residue	14	1	Phosphoserine Ref.4 Ref.5
	Modified residue	25	1	Phosphothreonine Ref.5
	Modified residue	107	1	Phosphoserine Ref.5
	Modified residue	109	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	137	1	P → L in AAB87902. Ref.1
	Sequence conflict	281	1	D → E in AAB87902. Ref.1
	Sequence conflict	309	1	L → F in AAB87902. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9BUQ8-1 [UniParc]. Last modified October 23, 2007. Version 3. Checksum: 01DD5BCF8BFBA2DB	FASTA	820	95,583
	10 20 30 40 50 60 MAGELADKKD RDASPSKEER KRSRTPDRER DRDRDRKSSP SKDRKRHRSR DRRRGGSRSR 70 80 90 100 110 120 SRSRSKSAER ERRHKERERD KERDRNKKDR DRDKDGHRRD KDRKRSSLSP GRGKDFKSRK 130 140 150 160 170 180 DRDSKKDEED EHGDKKPKAQ PLSLEELLAK KKAEEEAEAK PKFLSKAERE AEALKRRQQE 190 200 210 220 230 240 VEERQRMLEE ERKKRKQFQD LGRKMLEDPQ ERERRERRER MERETNGNED EEGRQKIREE 250 260 270 280 290 300 KDKSKELHAI KERYLGGIKK RRRTRHLNDR KFVFEWDASE DTSIDYNPLY KERHQVQLLG 310 320 330 340 350 360 RGFIAGIDLK QQKREQSRFY GDLMEKRRTL EEKEQEEARL RKLRKKEAKQ RWDDRHWSQK 370 380 390 400 410 420 KLDEMTDRDW RIFREDYSIT TKGGKIPNPI RSWKDSSLPP HILEVIDKCG YKEPTPIQRQ 430 440 450 460 470 480 AIPIGLQNRD IIGVAETGSG KTAAFLIPLL VWITTLPKID RIEESDQGPY AIILAPTREL 490 500 510 520 530 540 AQQIEEETIK FGKPLGIRTV AVIGGISRED QGFRLRMGCE IVIATPGRLI DVLENRYLVL 550 560 570 580 590 600 SRCTYVVLDE ADRMIDMGFE PDVQKILEHM PVSNQKPDTD EAEDPEKMLA NFESGKHKYR 610 620 630 640 650 660 QTVMFTATMP PAVERLARSY LRRPAVVYIG SAGKPHERVE QKVFLMSESE KRKKLLAILE 670 680 690 700 710 720 QGFDPPIIIF VNQKKGCDVL AKSLEKMGYN ACTLHGGKGQ EQREFALSNL KAGAKDILVA 730 740 750 760 770 780 TDVAGRGIDI QDVSMVVNYD MAKNIEDYIH RIGRTGRAGK SGVAITFLTK EDSAVFYELK 790 800 810 820 QAILESPVSS CPPELANHPD AQHKPGTILT KKRREETIFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human U5 snRNP-specific 100-kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p." Teigelkamp S., Mundt C., Achsel T., Will C.L., Luehrmann R. RNA 3:1313-1326(1997) [PubMed: 9409622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 272-290; 409-419 AND 433-441, SUBCELLULAR LOCATION, PHOSPHORYLATION, IDENTIFICATION IN U5 AND U5/4/6 SNRNP COMPLEXES.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed: 11991638] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPICEOSOMAL C COMPLEX.
[4]	"Global phosphoproteome of HT-29 human colon adenocarcinoma cells." Kim J.-E., Tannenbaum S.R., White F.M. J. Proteome Res. 4:1339-1346(2005) [PubMed: 16083285] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; THR-25; SER-107 AND SER-109, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF026402 mRNA. Translation: AAB87902.1. BC002366 mRNA. Translation: AAH02366.1.
IPI	IPI00006725.
RefSeq	NP_004809.2.
UniGene	Hs.130098
3D structure databases
HSSP	HSSP built from PDB template 1HV8 based on UniProtKB Q58083.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BUQ8. 4 interactions.
PTM databases
PhosphoSite	Q9BUQ8.
Proteomic databases
PRIDE	Q9BUQ8.
Genome annotation databases
Ensembl	ENSG00000174243. Homo sapiens. [Contig view]
GeneID	9416.
KEGG	hsa:9416.
Organism-specific databases
GeneCards	GC12M047509.
HGNC	HGNC:17347. DDX23.
PharmGKB	PA134934941.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9BUQ8.
HOVERGEN	Q9BUQ8.
OMA	Q9BUQ8. VQKILEY.
Enzyme and pathway databases
Reactome	REACT_1675. mRNA Processing. REACT_71. Gene Expression.
Gene expression databases
Bgee	Q9BUQ8.
CleanEx	HS_DDX23.
GermOnline	ENSG00000174243. Homo sapiens.
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	35278.

Entry information

Entry name

DDX23_HUMAN

Accession

Primary (citable) accession number: Q9BUQ8
Secondary accession number(s): O43188

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 72 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)