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Protein

Heterogeneous nuclear ribonucleoprotein U-like protein 1

Gene

HNRNPUL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.2 Publications

Miscellaneous

Its methylation is enhanced in the late phase of adenoviral infection.

Caution

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • RNA binding Source: UniProtKB

GO - Biological processi

  • mRNA splicing, via spliceosome Source: Reactome
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to virus Source: ProtInc
  • RNA processing Source: ProtInc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Repressor, Ribonucleoprotein, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein U-like protein 1
Alternative name(s):
Adenovirus early region 1B-associated protein 5
E1B-55 kDa-associated protein 5
Short name:
E1B-AP5
Gene namesi
Name:HNRNPUL1
Synonyms:E1BAP5, HNRPUL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105323.16
HGNCiHGNC:17011 HNRNPUL1
MIMi605800 gene
neXtProtiNX_Q9BUJ2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi11100
OpenTargetsiENSG00000105323
PharmGKBiPA162391519

Polymorphism and mutation databases

BioMutaiHNRNPUL1
DMDMi90101344

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002275551 – 856Heterogeneous nuclear ribonucleoprotein U-like protein 1Add BLAST856

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Cross-linki146Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki162Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei194PhosphoserineCombined sources1
Modified residuei209PhosphothreonineBy similarity1
Cross-linki270Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki449Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei512PhosphoserineCombined sources1
Cross-linki539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei639Asymmetric dimethylarginineCombined sources1
Modified residuei645Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei645Omega-N-methylarginine; alternateBy similarity1
Modified residuei656Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei656Omega-N-methylarginine; alternateBy similarity1
Modified residuei661Omega-N-methylarginineBy similarity1
Modified residuei671Omega-N-methylarginineCombined sources1
Modified residuei718PhosphoserineCombined sources1

Post-translational modificationi

Methylated.2 Publications

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9BUJ2
MaxQBiQ9BUJ2
PaxDbiQ9BUJ2
PeptideAtlasiQ9BUJ2
PRIDEiQ9BUJ2
TopDownProteomicsiQ9BUJ2-2 [Q9BUJ2-2]

PTM databases

iPTMnetiQ9BUJ2
PhosphoSitePlusiQ9BUJ2
SwissPalmiQ9BUJ2

Expressioni

Gene expression databases

BgeeiENSG00000105323
CleanExiHS_HNRNPUL1
ExpressionAtlasiQ9BUJ2 baseline and differential
GenevisibleiQ9BUJ2 HS

Organism-specific databases

HPAiCAB046477
HPA046290
HPA049475

Interactioni

Subunit structurei

Interacts with the adenovirus type 5 (Ad5) E1B-55 kDa, BRD7, PRMT2, TP53 and NXF1. Associates with histones and BRD7.5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • enzyme binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116281128 interactors.
CORUMiQ9BUJ2
DIPiDIP-39419N
IntActiQ9BUJ2 78 interactors.
MINTiQ9BUJ2
STRINGi9606.ENSP00000375863

Structurei

3D structure databases

ProteinModelPortaliQ9BUJ2
SMRiQ9BUJ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 37SAPPROSITE-ProRule annotationAdd BLAST35
Domaini191 – 388B30.2/SPRYPROSITE-ProRule annotationAdd BLAST198
Repeati612 – 6141-13
Repeati620 – 6221-23
Repeati639 – 6411-33
Repeati645 – 6471-43
Repeati656 – 6581-53

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 103Necessary for interaction with HRMT1L1Add BLAST103
Regioni213 – 856Necessary for interaction with TP531 PublicationAdd BLAST644
Regioni456 – 594Necessary for interaction with BRD7 and transcriptional activation1 PublicationAdd BLAST139
Regioni612 – 6585 X 3 AA repeats of R-G-GAdd BLAST47
Regioni612 – 658Necessary for transcription repressionAdd BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi613 – 666Gly-richAdd BLAST54
Compositional biasi670 – 689Asn-richAdd BLAST20
Compositional biasi692 – 811Pro-richAdd BLAST120
Compositional biasi757 – 845Tyr-richAdd BLAST89
Compositional biasi806 – 832Gln-richAdd BLAST27

Domaini

The RGG-box domain is methylated.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2242 Eukaryota
ENOG410XSBV LUCA
GeneTreeiENSGT00390000020210
HOVERGENiHBG061101
InParanoidiQ9BUJ2
KOiK15047
OMAiHYVMDNI
OrthoDBiEOG091G041T
PhylomeDBiQ9BUJ2
TreeFamiTF317301

Family and domain databases

CDDicd12884 SPRY_hnRNP, 1 hit
Gene3Di1.10.720.301 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR013320 ConA-like_dom_sf
IPR027025 hnRNP_U-like_1
IPR027417 P-loop_NTPase
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
IPR003877 SPRY_dom
IPR035778 SPRY_hnRNP_U
PANTHERiPTHR12381:SF41 PTHR12381:SF41, 1 hit
PfamiView protein in Pfam
PF02037 SAP, 1 hit
PF00622 SPRY, 1 hit
SMARTiView protein in SMART
SM00513 SAP, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
SSF52540 SSF52540, 1 hit
SSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS50800 SAP, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BUJ2-1) [UniParc]FASTAAdd to basket
Also known as: Isoform a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVRRLKVNE LREELQRRGL DTRGLKAELA ERLQAALEAE EPDDERELDA
60 70 80 90 100
DDEPGRPGHI NEEVETEGGS ELEGTAQPPP PGLQPHAEPG GYSGPDGHYA
110 120 130 140 150
MDNITRQNQF YDTQVIKQEN ESGYERRPLE MEQQQAYRPE MKTEMKQGAP
160 170 180 190 200
TSFLPPEASQ LKPDRQQFQS RKRPYEENRG RGYFEHREDR RGRSPQPPAE
210 220 230 240 250
EDEDDFDDTL VAIDTYNCDL HFKVARDRSS GYPLTIEGFA YLWSGARASY
260 270 280 290 300
GVRRGRVCFE MKINEEISVK HLPSTEPDPH VVRIGWSLDS CSTQLGEEPF
310 320 330 340 350
SYGYGGTGKK STNSRFENYG DKFAENDVIG CFADFECGND VELSFTKNGK
360 370 380 390 400
WMGIAFRIQK EALGGQALYP HVLVKNCAVE FNFGQRAEPY CSVLPGFTFI
410 420 430 440 450
QHLPLSERIR GTVGPKSKAE CEILMMVGLP AAGKTTWAIK HAASNPSKKY
460 470 480 490 500
NILGTNAIMD KMRVMGLRRQ RNYAGRWDVL IQQATQCLNR LIQIAARKKR
510 520 530 540 550
NYILDQTNVY GSAQRRKMRP FEGFQRKAIV ICPTDEDLKD RTIKRTDEEG
560 570 580 590 600
KDVPDHAVLE MKANFTLPDV GDFLDEVLFI ELQREEADKL VRQYNEEGRK
610 620 630 640 650
AGPPPEKRFD NRGGGGFRGR GGGGGFQRYE NRGPPGGNRG GFQNRGGGSG
660 670 680 690 700
GGGNYRGGFN RSGGGGYSQN RWGNNNRDNN NSNNRGSYNR APQQQPPPQQ
710 720 730 740 750
PPPPQPPPQQ PPPPPSYSPA RNPPGASTYN KNSNIPGSSA NTSTPTVSSY
760 770 780 790 800
SPPQPSYSQP PYNQGGYSQG YTAPPPPPPP PPAYNYGSYG GYNPAPYTPP
810 820 830 840 850
PPPTAQTYPQ PSYNQYQQYA QQWNQYYQNQ GQWPPYYGNY DYGSYSGNTQ

GGTSTQ
Length:856
Mass (Da):95,739
Last modified:March 21, 2006 - v2
Checksum:i6E57C0271E5F3A77
GO
Isoform 2 (identifier: Q9BUJ2-2) [UniParc]FASTAAdd to basket
Also known as: Isoform b

The sequence of this isoform differs from the canonical sequence as follows:
     755-806: Missing.

Show »
Length:804
Mass (Da):90,292
Checksum:i05950AF9FAFD192F
GO
Isoform 3 (identifier: Q9BUJ2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-77: Missing.
     263-333: Missing.
     754-754: Q → QSFGFFPSTFQ

Show »
Length:752
Mass (Da):84,500
Checksum:i207A6A67EFFFA299
GO
Isoform 4 (identifier: Q9BUJ2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Show »
Length:756
Mass (Da):84,794
Checksum:iB16C6EC86B997FFA
GO
Isoform 5 (identifier: Q9BUJ2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-460: Missing.
     461-562: KMRVMGLRRQ...VPDHAVLEMK → MGFCHVGQAG...CSLWGTSFLL
     754-754: Q → QSFGFFPSTFQ

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:390
Mass (Da):42,189
Checksum:iA86E13953F84279F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27A → T in CAA07548 (PubMed:9733834).Curated1
Sequence conflicti508N → S in BAC86806 (PubMed:14702039).Curated1
Sequence conflicti619G → A in CAA07548 (PubMed:9733834).Curated1
Sequence conflicti625G → A in CAA07548 (PubMed:9733834).Curated1
Sequence conflicti662S → N in CAA07548 (PubMed:9733834).Curated1
Sequence conflicti691A → S in BAC86806 (PubMed:14702039).Curated1
Sequence conflicti773A → G in CAA07548 (PubMed:9733834).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02560691G → C1 PublicationCorresponds to variant dbSNP:rs17849624Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0175461 – 460Missing in isoform 5. 1 PublicationAdd BLAST460
Alternative sequenceiVSP_0175471 – 100Missing in isoform 4. 1 PublicationAdd BLAST100
Alternative sequenceiVSP_01754835 – 77Missing in isoform 3. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_017549263 – 333Missing in isoform 3. 1 PublicationAdd BLAST71
Alternative sequenceiVSP_017550461 – 562KMRVM…VLEMK → MGFCHVGQAGLELLTSGDPP ASASQSAGITGVSHRARPSV FVFLIHYSSFLHLLPSGRPL FWVEGTRLQKVLTSSSCSLW GTSFLL in isoform 5. 1 PublicationAdd BLAST102
Alternative sequenceiVSP_017551754Q → QSFGFFPSTFQ in isoform 3 and isoform 5. 1 Publication1
Alternative sequenceiVSP_017552755 – 806Missing in isoform 2. 1 PublicationAdd BLAST52

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ007509 mRNA Translation: CAA07548.1
AK021455 mRNA Translation: BAB13831.1
AK022863 mRNA Translation: BAG51129.1
AK127057 mRNA Translation: BAC86806.1
AC011462 Genomic DNA No translation available.
AC011510 Genomic DNA No translation available.
CH471126 Genomic DNA Translation: EAW57025.1
BC002564 mRNA Translation: AAH02564.1
BC009988 mRNA Translation: AAH09988.2
BC014232 mRNA Translation: AAH14232.1
BC027713 mRNA Translation: AAH27713.1
AL050146 mRNA Translation: CAB43291.1
CCDSiCCDS12576.1 [Q9BUJ2-1]
CCDS12577.1 [Q9BUJ2-4]
PIRiT08776
T13159
RefSeqiNP_001308137.1, NM_001321208.1 [Q9BUJ2-4]
NP_001308140.1, NM_001321211.1 [Q9BUJ2-4]
NP_008971.2, NM_007040.5 [Q9BUJ2-1]
NP_653333.1, NM_144732.4 [Q9BUJ2-4]
UniGeneiHs.155218
Hs.718642

Genome annotation databases

EnsembliENST00000378215; ENSP00000367460; ENSG00000105323 [Q9BUJ2-3]
ENST00000392006; ENSP00000375863; ENSG00000105323 [Q9BUJ2-1]
ENST00000593587; ENSP00000472629; ENSG00000105323 [Q9BUJ2-4]
ENST00000595018; ENSP00000473132; ENSG00000105323 [Q9BUJ2-4]
ENST00000602130; ENSP00000470687; ENSG00000105323 [Q9BUJ2-2]
GeneIDi11100
KEGGihsa:11100
UCSCiuc002opz.5 human [Q9BUJ2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiHNRL1_HUMAN
AccessioniPrimary (citable) accession number: Q9BUJ2
Secondary accession number(s): B3KMW7
, O76022, Q6ZSZ0, Q7L8P4, Q8N6Z4, Q96G37, Q9HAL3, Q9UG75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: April 25, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome