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Protein

Protein crumbs homolog 3

Gene

CRB3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions.2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: BHF-UCL
  • SH3 domain binding Source: BHF-UCL

GO - Biological processi

  • bicellular tight junction assembly Source: Reactome
  • protein localization to plasma membrane Source: BHF-UCL
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130545-MONOMER.
ReactomeiR-HSA-420029. Tight junction interactions.
SIGNORiQ9BUF7.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein crumbs homolog 3
Gene namesi
Name:CRB3
ORF Names:UNQ588/PRO1158
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:20237. CRB3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 59ExtracellularSequence analysisAdd BLAST33
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 120CytoplasmicSequence analysisAdd BLAST40

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36N → D: Abolishes N-glycosylation. 1 Publication1
Mutagenesisi117 – 120Missing : Loss of interaction with PARD6A and MPP5. 2 Publications4

Organism-specific databases

DisGeNETi92359.
OpenTargetsiENSG00000130545.
PharmGKBiPA134862130.

Polymorphism and mutation databases

BioMutaiCRB3.
DMDMi67460584.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000002100527 – 120Protein crumbs homolog 3Add BLAST94

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi36N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9BUF7.
MaxQBiQ9BUF7.
PeptideAtlasiQ9BUF7.
PRIDEiQ9BUF7.

PTM databases

iPTMnetiQ9BUF7.
PhosphoSitePlusiQ9BUF7.

Expressioni

Tissue specificityi

Preferentially expressed in epithelial tissues. Expressed at high levels in lung, kidney, retina, colon and mammary glands. Expressed at moderate levels in liver, spleen, pancreas, placenta and prostate.2 Publications

Gene expression databases

BgeeiENSG00000130545.
CleanExiHS_CRB3.
GenevisibleiQ9BUF7. HS.

Organism-specific databases

HPAiHPA013835.

Interactioni

Subunit structurei

Interacts with MPP5, PARD6A and PATJ.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PSMA3P257883EBI-9844372,EBI-348380

GO - Molecular functioni

  • protein domain specific binding Source: BHF-UCL
  • SH3 domain binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi124940. 3 interactors.
IntActiQ9BUF7. 2 interactors.

Structurei

Secondary structure

1120
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi116 – 120Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5I7ZX-ray1.80B113-120[»]
ProteinModelPortaliQ9BUF7.
SMRiQ9BUF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi117 – 120PDZ-binding4

Domaini

The PDZ-binding motif is involved in the interactions with PARD6A and MPP5.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000012998.
HOGENOMiHOG000013209.
HOVERGENiHBG081347.
InParanoidiQ9BUF7.
KOiK06090.
OMAiWGQVWGQ.
OrthoDBiEOG091G14HT.
PhylomeDBiQ9BUF7.
TreeFamiTF353183.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BUF7-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MANPGLGLLL ALGLPFLLAR WGRAWGQIQT TSANENSTVL PSSTSSSSDG
60 70 80 90 100
NLRPEAITAI IVVFSLLAAL LLAVGLALLV RKLREKRQTE GTYRPSSEEQ
110 120
VGARVPPTPN LKLPPEERLI
Length:120
Mass (Da):12,854
Last modified:June 7, 2005 - v3
Checksum:iF0CCDE3D91E7392D
GO
Isoform 2 (identifier: Q9BUF7-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     101-120: VGARVPPTPNLKLPPEERLI → FSHAAEARAPQDSKETVQGCLPI

Show »
Length:123
Mass (Da):13,113
Checksum:iD43DADF94F36303E
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013989101 – 120VGARV…EERLI → FSHAAEARAPQDSKETVQGC LPI in isoform 2. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY103469 mRNA. Translation: AAM44074.1.
AF503290 mRNA. Translation: AAM23013.1.
AY358684 mRNA. Translation: AAQ89047.1.
AK292956 mRNA. Translation: BAF85645.1.
CH471139 Genomic DNA. Translation: EAW69084.1.
CH471139 Genomic DNA. Translation: EAW69085.1.
CH471139 Genomic DNA. Translation: EAW69086.1.
BC002652 mRNA. Translation: AAH02652.2.
BC018409 mRNA. Translation: AAH18409.1.
CCDSiCCDS12166.1. [Q9BUF7-2]
CCDS12167.1. [Q9BUF7-1]
RefSeqiNP_631900.1. NM_139161.4. [Q9BUF7-1]
NP_777377.1. NM_174881.3. [Q9BUF7-2]
NP_777378.1. NM_174882.2. [Q9BUF7-1]
UniGeneiHs.150319.

Genome annotation databases

EnsembliENST00000308243; ENSP00000310123; ENSG00000130545. [Q9BUF7-1]
ENST00000356762; ENSP00000349204; ENSG00000130545. [Q9BUF7-2]
ENST00000598494; ENSP00000469707; ENSG00000130545. [Q9BUF7-1]
ENST00000600229; ENSP00000472010; ENSG00000130545. [Q9BUF7-1]
GeneIDi92359.
KEGGihsa:92359.
UCSCiuc002mey.4. human. [Q9BUF7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY103469 mRNA. Translation: AAM44074.1.
AF503290 mRNA. Translation: AAM23013.1.
AY358684 mRNA. Translation: AAQ89047.1.
AK292956 mRNA. Translation: BAF85645.1.
CH471139 Genomic DNA. Translation: EAW69084.1.
CH471139 Genomic DNA. Translation: EAW69085.1.
CH471139 Genomic DNA. Translation: EAW69086.1.
BC002652 mRNA. Translation: AAH02652.2.
BC018409 mRNA. Translation: AAH18409.1.
CCDSiCCDS12166.1. [Q9BUF7-2]
CCDS12167.1. [Q9BUF7-1]
RefSeqiNP_631900.1. NM_139161.4. [Q9BUF7-1]
NP_777377.1. NM_174881.3. [Q9BUF7-2]
NP_777378.1. NM_174882.2. [Q9BUF7-1]
UniGeneiHs.150319.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5I7ZX-ray1.80B113-120[»]
ProteinModelPortaliQ9BUF7.
SMRiQ9BUF7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124940. 3 interactors.
IntActiQ9BUF7. 2 interactors.

PTM databases

iPTMnetiQ9BUF7.
PhosphoSitePlusiQ9BUF7.

Polymorphism and mutation databases

BioMutaiCRB3.
DMDMi67460584.

Proteomic databases

EPDiQ9BUF7.
MaxQBiQ9BUF7.
PeptideAtlasiQ9BUF7.
PRIDEiQ9BUF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308243; ENSP00000310123; ENSG00000130545. [Q9BUF7-1]
ENST00000356762; ENSP00000349204; ENSG00000130545. [Q9BUF7-2]
ENST00000598494; ENSP00000469707; ENSG00000130545. [Q9BUF7-1]
ENST00000600229; ENSP00000472010; ENSG00000130545. [Q9BUF7-1]
GeneIDi92359.
KEGGihsa:92359.
UCSCiuc002mey.4. human. [Q9BUF7-1]

Organism-specific databases

CTDi92359.
DisGeNETi92359.
GeneCardsiCRB3.
HGNCiHGNC:20237. CRB3.
HPAiHPA013835.
MIMi609737. gene.
neXtProtiNX_Q9BUF7.
OpenTargetsiENSG00000130545.
PharmGKBiPA134862130.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000012998.
HOGENOMiHOG000013209.
HOVERGENiHBG081347.
InParanoidiQ9BUF7.
KOiK06090.
OMAiWGQVWGQ.
OrthoDBiEOG091G14HT.
PhylomeDBiQ9BUF7.
TreeFamiTF353183.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130545-MONOMER.
ReactomeiR-HSA-420029. Tight junction interactions.
SIGNORiQ9BUF7.

Miscellaneous databases

GeneWikiiCRB3.
GenomeRNAii92359.
PROiQ9BUF7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130545.
CleanExiHS_CRB3.
GenevisibleiQ9BUF7. HS.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiCRUM3_HUMAN
AccessioniPrimary (citable) accession number: Q9BUF7
Secondary accession number(s): A8KA91
, D6W643, Q8N0V8, Q8WVA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.