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Protein

Mediator of RNA polymerase II transcription subunit 18

Gene

MED18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

  1. RNA polymerase II transcription cofactor activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 18
Alternative name(s):
Mediator complex subunit 18
p28b
Gene namesi
Name:MED18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:25944. MED18.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. mediator complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134884523.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208Mediator of RNA polymerase II transcription subunit 18PRO_0000304742Add
BLAST

Proteomic databases

MaxQBiQ9BUE0.
PaxDbiQ9BUE0.
PeptideAtlasiQ9BUE0.
PRIDEiQ9BUE0.

PTM databases

PhosphoSiteiQ9BUE0.

Expressioni

Gene expression databases

BgeeiQ9BUE0.
CleanExiHS_MED18.
ExpressionAtlasiQ9BUE0. baseline and differential.
GenevestigatoriQ9BUE0.

Organism-specific databases

HPAiHPA027429.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Med20Q9R0X06EBI-394640,EBI-398698From a different organism.
MED22Q155282EBI-394640,EBI-394687
MED29Q9NX705EBI-394640,EBI-394656

Protein-protein interaction databases

BioGridi120156. 39 interactions.
IntActiQ9BUE0. 15 interactions.
STRINGi9606.ENSP00000362948.

Structurei

3D structure databases

ProteinModelPortaliQ9BUE0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 18 family.Curated

Phylogenomic databases

eggNOGiNOG262405.
GeneTreeiENSGT00390000003312.
HOVERGENiHBG105560.
InParanoidiQ9BUE0.
KOiK15135.
OMAiMANEPIS.
OrthoDBiEOG7XH6QZ.
PhylomeDBiQ9BUE0.
TreeFamiTF313246.

Family and domain databases

InterProiIPR019095. Mediator_Med18_met/fun.
[Graphical view]
PfamiPF09637. Med18. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BUE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET
60 70 80 90 100
FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLGQ PEMGDKNRHA
110 120 130 140 150
LVRNCVDIAT SENLTDFLME MGFRMDHEFV AKGHLFRKGI MKIMVYKIFR
160 170 180 190 200
ILVPGNTDST EALSLSYLVE LSVVAPAGQD MVSDDMKNFA EQLKPLVHLE

KIDPKRLM
Length:208
Mass (Da):23,663
Last modified:June 1, 2001 - v1
Checksum:iAE68984390532C33
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti148 – 1481I → T in BAA90910 (PubMed:14702039).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB107222 mRNA. Translation: BAD06869.1.
AK000052 mRNA. Translation: BAA90910.1.
AL353622 Genomic DNA. Translation: CAI19132.1.
CH471059 Genomic DNA. Translation: EAX07699.1.
CH471059 Genomic DNA. Translation: EAX07700.1.
CH471059 Genomic DNA. Translation: EAX07702.1.
BC002694 mRNA. Translation: AAH02694.1.
CCDSiCCDS322.1.
RefSeqiNP_001120822.1. NM_001127350.1.
NP_060108.2. NM_017638.2.
XP_005245971.1. XM_005245914.2.
UniGeneiHs.479911.

Genome annotation databases

EnsembliENST00000373842; ENSP00000362948; ENSG00000130772.
ENST00000398997; ENSP00000381963; ENSG00000130772.
GeneIDi54797.
KEGGihsa:54797.
UCSCiuc001bpt.4. human.

Polymorphism databases

DMDMi74752353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB107222 mRNA. Translation: BAD06869.1.
AK000052 mRNA. Translation: BAA90910.1.
AL353622 Genomic DNA. Translation: CAI19132.1.
CH471059 Genomic DNA. Translation: EAX07699.1.
CH471059 Genomic DNA. Translation: EAX07700.1.
CH471059 Genomic DNA. Translation: EAX07702.1.
BC002694 mRNA. Translation: AAH02694.1.
CCDSiCCDS322.1.
RefSeqiNP_001120822.1. NM_001127350.1.
NP_060108.2. NM_017638.2.
XP_005245971.1. XM_005245914.2.
UniGeneiHs.479911.

3D structure databases

ProteinModelPortaliQ9BUE0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120156. 39 interactions.
IntActiQ9BUE0. 15 interactions.
STRINGi9606.ENSP00000362948.

PTM databases

PhosphoSiteiQ9BUE0.

Polymorphism databases

DMDMi74752353.

Proteomic databases

MaxQBiQ9BUE0.
PaxDbiQ9BUE0.
PeptideAtlasiQ9BUE0.
PRIDEiQ9BUE0.

Protocols and materials databases

DNASUi54797.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373842; ENSP00000362948; ENSG00000130772.
ENST00000398997; ENSP00000381963; ENSG00000130772.
GeneIDi54797.
KEGGihsa:54797.
UCSCiuc001bpt.4. human.

Organism-specific databases

CTDi54797.
GeneCardsiGC01P028666.
H-InvDBHIX0000293.
HGNCiHGNC:25944. MED18.
HPAiHPA027429.
MIMi612384. gene.
neXtProtiNX_Q9BUE0.
PharmGKBiPA134884523.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG262405.
GeneTreeiENSGT00390000003312.
HOVERGENiHBG105560.
InParanoidiQ9BUE0.
KOiK15135.
OMAiMANEPIS.
OrthoDBiEOG7XH6QZ.
PhylomeDBiQ9BUE0.
TreeFamiTF313246.

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

GenomeRNAii54797.
NextBioi57479.
PROiQ9BUE0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BUE0.
CleanExiHS_MED18.
ExpressionAtlasiQ9BUE0. baseline and differential.
GenevestigatoriQ9BUE0.

Family and domain databases

InterProiIPR019095. Mediator_Med18_met/fun.
[Graphical view]
PfamiPF09637. Med18. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Physical and functional connections between human Mediator complex and general transcription machinery."
    Furumoto T., Malik S., Hayashi K., Tanaka A., Ito M., Roeder R.G., Hanaoka F., Ohkuma Y.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lymphoma.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Uterus.
  6. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
    Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 278:15123-15127(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED10 AND MED20.
  7. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  8. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCNC; MED1; MED12; MED13; MED17; MED20 AND MED21, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

Entry informationi

Entry nameiMED18_HUMAN
AccessioniPrimary (citable) accession number: Q9BUE0
Secondary accession number(s): D3DPM1, Q9NXU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.