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Protein

Mediator of RNA polymerase II transcription subunit 18

Gene

MED18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130772-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 18
Alternative name(s):
Mediator complex subunit 18
p28b
Gene namesi
Name:MED18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:25944. MED18.

Subcellular locationi

GO - Cellular componenti

  • core mediator complex Source: GO_Central
  • mediator complex Source: MGI
  • ubiquitin ligase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000130772.
PharmGKBiPA134884523.

Polymorphism and mutation databases

BioMutaiMED18.
DMDMi74752353.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003047421 – 208Mediator of RNA polymerase II transcription subunit 18Add BLAST208

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BUE0.
MaxQBiQ9BUE0.
PaxDbiQ9BUE0.
PeptideAtlasiQ9BUE0.
PRIDEiQ9BUE0.

PTM databases

iPTMnetiQ9BUE0.
PhosphoSitePlusiQ9BUE0.

Expressioni

Gene expression databases

BgeeiENSG00000130772.
CleanExiHS_MED18.
GenevisibleiQ9BUE0. HS.

Organism-specific databases

HPAiHPA027429.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAMK2BQ135543EBI-394640,EBI-1058722
HOMEZQ8IX15-33EBI-394640,EBI-10172004
MED20Q9H9449EBI-394640,EBI-394644
Med20Q9R0X06EBI-394640,EBI-398698From a different organism.
MED22Q155282EBI-394640,EBI-394687
MED29Q9NX705EBI-394640,EBI-394656
TRIM39Q9HCM93EBI-394640,EBI-739510

Protein-protein interaction databases

BioGridi120156. 47 interactors.
IntActiQ9BUE0. 22 interactors.
STRINGi9606.ENSP00000362948.

Structurei

3D structure databases

ProteinModelPortaliQ9BUE0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 18 family.Curated

Phylogenomic databases

eggNOGiKOG3264. Eukaryota.
ENOG4110KT9. LUCA.
GeneTreeiENSGT00390000003312.
HOVERGENiHBG105560.
InParanoidiQ9BUE0.
KOiK15135.
OMAiRGYMFRK.
OrthoDBiEOG091G0K0D.
PhylomeDBiQ9BUE0.
TreeFamiTF313246.

Family and domain databases

InterProiIPR019095. Mediator_Med18_met/fun.
[Graphical view]
PfamiPF09637. Med18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BUE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET
60 70 80 90 100
FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLGQ PEMGDKNRHA
110 120 130 140 150
LVRNCVDIAT SENLTDFLME MGFRMDHEFV AKGHLFRKGI MKIMVYKIFR
160 170 180 190 200
ILVPGNTDST EALSLSYLVE LSVVAPAGQD MVSDDMKNFA EQLKPLVHLE

KIDPKRLM
Length:208
Mass (Da):23,663
Last modified:June 1, 2001 - v1
Checksum:iAE68984390532C33
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti148I → T in BAA90910 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB107222 mRNA. Translation: BAD06869.1.
AK000052 mRNA. Translation: BAA90910.1.
AL353622 Genomic DNA. Translation: CAI19132.1.
CH471059 Genomic DNA. Translation: EAX07699.1.
CH471059 Genomic DNA. Translation: EAX07700.1.
CH471059 Genomic DNA. Translation: EAX07702.1.
BC002694 mRNA. Translation: AAH02694.1.
CCDSiCCDS322.1.
RefSeqiNP_001120822.1. NM_001127350.1.
NP_060108.2. NM_017638.2.
XP_005245971.1. XM_005245914.4.
UniGeneiHs.479911.

Genome annotation databases

EnsembliENST00000373842; ENSP00000362948; ENSG00000130772.
ENST00000398997; ENSP00000381963; ENSG00000130772.
GeneIDi54797.
KEGGihsa:54797.
UCSCiuc001bpt.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB107222 mRNA. Translation: BAD06869.1.
AK000052 mRNA. Translation: BAA90910.1.
AL353622 Genomic DNA. Translation: CAI19132.1.
CH471059 Genomic DNA. Translation: EAX07699.1.
CH471059 Genomic DNA. Translation: EAX07700.1.
CH471059 Genomic DNA. Translation: EAX07702.1.
BC002694 mRNA. Translation: AAH02694.1.
CCDSiCCDS322.1.
RefSeqiNP_001120822.1. NM_001127350.1.
NP_060108.2. NM_017638.2.
XP_005245971.1. XM_005245914.4.
UniGeneiHs.479911.

3D structure databases

ProteinModelPortaliQ9BUE0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120156. 47 interactors.
IntActiQ9BUE0. 22 interactors.
STRINGi9606.ENSP00000362948.

PTM databases

iPTMnetiQ9BUE0.
PhosphoSitePlusiQ9BUE0.

Polymorphism and mutation databases

BioMutaiMED18.
DMDMi74752353.

Proteomic databases

EPDiQ9BUE0.
MaxQBiQ9BUE0.
PaxDbiQ9BUE0.
PeptideAtlasiQ9BUE0.
PRIDEiQ9BUE0.

Protocols and materials databases

DNASUi54797.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373842; ENSP00000362948; ENSG00000130772.
ENST00000398997; ENSP00000381963; ENSG00000130772.
GeneIDi54797.
KEGGihsa:54797.
UCSCiuc001bpt.5. human.

Organism-specific databases

CTDi54797.
GeneCardsiMED18.
H-InvDBHIX0000293.
HGNCiHGNC:25944. MED18.
HPAiHPA027429.
MIMi612384. gene.
neXtProtiNX_Q9BUE0.
OpenTargetsiENSG00000130772.
PharmGKBiPA134884523.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3264. Eukaryota.
ENOG4110KT9. LUCA.
GeneTreeiENSGT00390000003312.
HOVERGENiHBG105560.
InParanoidiQ9BUE0.
KOiK15135.
OMAiRGYMFRK.
OrthoDBiEOG091G0K0D.
PhylomeDBiQ9BUE0.
TreeFamiTF313246.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130772-MONOMER.
ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

GenomeRNAii54797.
PROiQ9BUE0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130772.
CleanExiHS_MED18.
GenevisibleiQ9BUE0. HS.

Family and domain databases

InterProiIPR019095. Mediator_Med18_met/fun.
[Graphical view]
PfamiPF09637. Med18. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED18_HUMAN
AccessioniPrimary (citable) accession number: Q9BUE0
Secondary accession number(s): D3DPM1, Q9NXU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.