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Q9BUE0

- MED18_HUMAN

UniProt

Q9BUE0 - MED18_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 18

Gene

MED18

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA polymerase II transcription cofactor activity Source: InterPro

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 18
    Alternative name(s):
    Mediator complex subunit 18
    p28b
    Gene namesi
    Name:MED18
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:25944. MED18.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134884523.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 208208Mediator of RNA polymerase II transcription subunit 18PRO_0000304742Add
    BLAST

    Proteomic databases

    MaxQBiQ9BUE0.
    PaxDbiQ9BUE0.
    PeptideAtlasiQ9BUE0.
    PRIDEiQ9BUE0.

    PTM databases

    PhosphoSiteiQ9BUE0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9BUE0.
    BgeeiQ9BUE0.
    CleanExiHS_MED18.
    GenevestigatoriQ9BUE0.

    Organism-specific databases

    HPAiHPA027429.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Med20Q9R0X04EBI-394640,EBI-398698From a different organism.

    Protein-protein interaction databases

    BioGridi120156. 36 interactions.
    IntActiQ9BUE0. 9 interactions.
    STRINGi9606.ENSP00000362948.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BUE0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 18 family.Curated

    Phylogenomic databases

    eggNOGiNOG262405.
    HOVERGENiHBG105560.
    InParanoidiQ9BUE0.
    KOiK15135.
    OMAiVQLEKID.
    OrthoDBiEOG7XH6QZ.
    PhylomeDBiQ9BUE0.
    TreeFamiTF313246.

    Family and domain databases

    InterProiIPR019095. Mediator_Med18_met/fun.
    [Graphical view]
    PfamiPF09637. Med18. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9BUE0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEAPPVTMMP VTGGTINMME YLLQGSVLDH SLESLIHRLR GLCDNMEPET    50
    FLDHEMVFLL KGQQASPFVL RARRSMDRAG APWHLRYLGQ PEMGDKNRHA 100
    LVRNCVDIAT SENLTDFLME MGFRMDHEFV AKGHLFRKGI MKIMVYKIFR 150
    ILVPGNTDST EALSLSYLVE LSVVAPAGQD MVSDDMKNFA EQLKPLVHLE 200
    KIDPKRLM 208
    Length:208
    Mass (Da):23,663
    Last modified:June 1, 2001 - v1
    Checksum:iAE68984390532C33
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti148 – 1481I → T in BAA90910. (PubMed:14702039)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB107222 mRNA. Translation: BAD06869.1.
    AK000052 mRNA. Translation: BAA90910.1.
    AL353622 Genomic DNA. Translation: CAI19132.1.
    CH471059 Genomic DNA. Translation: EAX07699.1.
    CH471059 Genomic DNA. Translation: EAX07700.1.
    CH471059 Genomic DNA. Translation: EAX07702.1.
    BC002694 mRNA. Translation: AAH02694.1.
    CCDSiCCDS322.1.
    RefSeqiNP_001120822.1. NM_001127350.1.
    NP_060108.2. NM_017638.2.
    XP_005245971.1. XM_005245914.2.
    UniGeneiHs.479911.

    Genome annotation databases

    EnsembliENST00000373842; ENSP00000362948; ENSG00000130772.
    ENST00000398997; ENSP00000381963; ENSG00000130772.
    GeneIDi54797.
    KEGGihsa:54797.
    UCSCiuc001bpt.4. human.

    Polymorphism databases

    DMDMi74752353.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB107222 mRNA. Translation: BAD06869.1 .
    AK000052 mRNA. Translation: BAA90910.1 .
    AL353622 Genomic DNA. Translation: CAI19132.1 .
    CH471059 Genomic DNA. Translation: EAX07699.1 .
    CH471059 Genomic DNA. Translation: EAX07700.1 .
    CH471059 Genomic DNA. Translation: EAX07702.1 .
    BC002694 mRNA. Translation: AAH02694.1 .
    CCDSi CCDS322.1.
    RefSeqi NP_001120822.1. NM_001127350.1.
    NP_060108.2. NM_017638.2.
    XP_005245971.1. XM_005245914.2.
    UniGenei Hs.479911.

    3D structure databases

    ProteinModelPortali Q9BUE0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120156. 36 interactions.
    IntActi Q9BUE0. 9 interactions.
    STRINGi 9606.ENSP00000362948.

    PTM databases

    PhosphoSitei Q9BUE0.

    Polymorphism databases

    DMDMi 74752353.

    Proteomic databases

    MaxQBi Q9BUE0.
    PaxDbi Q9BUE0.
    PeptideAtlasi Q9BUE0.
    PRIDEi Q9BUE0.

    Protocols and materials databases

    DNASUi 54797.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000373842 ; ENSP00000362948 ; ENSG00000130772 .
    ENST00000398997 ; ENSP00000381963 ; ENSG00000130772 .
    GeneIDi 54797.
    KEGGi hsa:54797.
    UCSCi uc001bpt.4. human.

    Organism-specific databases

    CTDi 54797.
    GeneCardsi GC01P028666.
    H-InvDB HIX0000293.
    HGNCi HGNC:25944. MED18.
    HPAi HPA027429.
    MIMi 612384. gene.
    neXtProti NX_Q9BUE0.
    PharmGKBi PA134884523.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG262405.
    HOVERGENi HBG105560.
    InParanoidi Q9BUE0.
    KOi K15135.
    OMAi VQLEKID.
    OrthoDBi EOG7XH6QZ.
    PhylomeDBi Q9BUE0.
    TreeFami TF313246.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    GenomeRNAii 54797.
    NextBioi 57479.
    PROi Q9BUE0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BUE0.
    Bgeei Q9BUE0.
    CleanExi HS_MED18.
    Genevestigatori Q9BUE0.

    Family and domain databases

    InterProi IPR019095. Mediator_Med18_met/fun.
    [Graphical view ]
    Pfami PF09637. Med18. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Physical and functional connections between human Mediator complex and general transcription machinery."
      Furumoto T., Malik S., Hayashi K., Tanaka A., Ito M., Roeder R.G., Hanaoka F., Ohkuma Y.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lymphoma.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    3. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Uterus.
    6. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
      Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
      J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED10 AND MED20.
    7. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
    8. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CCNC; MED1; MED12; MED13; MED17; MED20 AND MED21, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.

    Entry informationi

    Entry nameiMED18_HUMAN
    AccessioniPrimary (citable) accession number: Q9BUE0
    Secondary accession number(s): D3DPM1, Q9NXU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3