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Protein

Spondin-2

Gene

SPON2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Divalent metal cationSequence analysis1
Metal bindingi122Divalent metal cationSequence analysis1
Metal bindingi141Divalent metal cationCurated1
Sitei141Important for metal ion-dependent interaction with integrin1 Publication1
Metal bindingi160CalciumCombined sources1 Publication1
Metal bindingi188CalciumCombined sources1 Publication1
Metal bindingi192Calcium; via carbonyl oxygenCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-5083635. Defective B3GALTL causes Peters-plus syndrome (PpS).
R-HSA-5173214. O-glycosylation of TSR domain-containing proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Spondin-2
Alternative name(s):
Differentially expressed in cancerous and non-cancerous lung cells 1
Short name:
DIL-1
Mindin
Gene namesi
Name:SPON2
Synonyms:DIL1
ORF Names:UNQ435/PRO866
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:11253. SPON2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi122E → A: Strongly reduced metal-dependent interaction with integrin; when associated with A-141. 1 Publication1
Mutagenesisi141E → A: Strongly reduced metal-dependent interaction with integrin; when associated with A-122. 1 Publication1

Organism-specific databases

DisGeNETi10417.
PharmGKBiPA36083.

Polymorphism and mutation databases

BioMutaiSPON2.
DMDMi313104285.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000003587027 – 331Spondin-2Add BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi35 ↔ 171PROSITE-ProRule annotation1 Publication
Glycosylationi283C-linked (Man)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9BUD6.
PaxDbiQ9BUD6.
PeptideAtlasiQ9BUD6.
PRIDEiQ9BUD6.

PTM databases

iPTMnetiQ9BUD6.
PhosphoSitePlusiQ9BUD6.

Expressioni

Tissue specificityi

Expressed in normal lung tissue but not in lung carcinoma cell lines.1 Publication

Gene expression databases

BgeeiENSG00000159674.
CleanExiHS_SPON2.
ExpressionAtlasiQ9BUD6. baseline and differential.
GenevisibleiQ9BUD6. HS.

Organism-specific databases

HPAiHPA040170.
HPA043890.
HPA066095.

Interactioni

Subunit structurei

Monomer. Interacts with integrin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT31Q153235EBI-10298801,EBI-948001

Protein-protein interaction databases

BioGridi115686. 1 interactor.
IntActiQ9BUD6. 1 interactor.
STRINGi9606.ENSP00000290902.

Structurei

Secondary structure

1331
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 49Combined sources9
Turni53 – 55Combined sources3
Beta strandi63 – 65Combined sources3
Beta strandi67 – 77Combined sources11
Helixi92 – 100Combined sources9
Helixi104 – 117Combined sources14
Beta strandi120 – 126Combined sources7
Beta strandi129 – 131Combined sources3
Beta strandi135 – 143Combined sources9
Beta strandi148 – 157Combined sources10
Beta strandi159 – 169Combined sources11
Beta strandi178 – 184Combined sources7
Beta strandi190 – 192Combined sources3
Beta strandi215 – 218Combined sources4
Beta strandi230 – 232Combined sources3
Beta strandi236 – 243Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D34X-ray1.80A/B27-249[»]
ProteinModelPortaliQ9BUD6.
SMRiQ9BUD6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BUD6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 221SpondinPROSITE-ProRule annotationAdd BLAST191
Domaini277 – 331TSP type-1PROSITE-ProRule annotationAdd BLAST55

Sequence similaritiesi

Contains 1 spondin domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IR0P. Eukaryota.
ENOG410XX1S. LUCA.
HOGENOMiHOG000114626.
HOVERGENiHBG052919.
InParanoidiQ9BUD6.
OrthoDBiEOG091G0BT5.
PhylomeDBiQ9BUD6.
TreeFamiTF326913.

Family and domain databases

InterProiIPR009465. Spondin_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF06468. Spond_N. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
SMARTiSM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 1 hit.
PROSITEiPS51020. SPONDIN. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BUD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENPSPAAAL GKALCALLLA TLGAAGQPLG GESICSARAL AKYSITFTGK
60 70 80 90 100
WSQTAFPKQY PLFRPPAQWS SLLGAAHSSD YSMWRKNQYV SNGLRDFAER
110 120 130 140 150
GEAWALMKEI EAAGEALQSV HEVFSAPAVP SGTGQTSAEL EVQRRHSLVS
160 170 180 190 200
FVVRIVPSPD WFVGVDSLDL CDGDRWREQA ALDLYPYDAG TDSGFTFSSP
210 220 230 240 250
NFATIPQDTV TEITSSSPSH PANSFYYPRL KALPPIARVT LVRLRQSPRA
260 270 280 290 300
FIPPAPVLPS RDNEIVDSAS VPETPLDCEV SLWSSWGLCG GHCGRLGTKS
310 320 330
RTRYVRVQPA NNGSPCPELE EEAECVPDNC V
Length:331
Mass (Da):35,846
Last modified:November 30, 2010 - v3
Checksum:i8AF827142D921705
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05514938R → G.Corresponds to variant rs6836335dbSNPEnsembl.1
Natural variantiVAR_01970140L → P.5 PublicationsCorresponds to variant rs922697dbSNPEnsembl.1
Natural variantiVAR_019702122E → A.4 PublicationsCorresponds to variant rs11247975dbSNPEnsembl.1
Natural variantiVAR_019703242V → L.4 PublicationsCorresponds to variant rs2279279dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027466 mRNA. Translation: BAA85892.1.
AY358948 mRNA. Translation: AAQ89307.1.
AK074618 mRNA. Translation: BAC11092.1.
AK074770 mRNA. Translation: BAC11196.1.
AC092535 Genomic DNA. Translation: AAY40988.1.
CH471131 Genomic DNA. Translation: EAW82604.1.
CH471131 Genomic DNA. Translation: EAW82606.1.
BC002707 mRNA. Translation: AAH02707.1.
BC036341 mRNA. Translation: AAH36341.1.
CCDSiCCDS3347.1.
RefSeqiNP_001121797.1. NM_001128325.2.
NP_001185950.1. NM_001199021.1.
NP_036577.1. NM_012445.3.
UniGeneiHs.302963.
Hs.635350.
Hs.736956.

Genome annotation databases

EnsembliENST00000290902; ENSP00000290902; ENSG00000159674.
ENST00000431380; ENSP00000394832; ENSG00000159674.
ENST00000617421; ENSP00000483599; ENSG00000159674.
GeneIDi10417.
KEGGihsa:10417.
UCSCiuc003gco.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027466 mRNA. Translation: BAA85892.1.
AY358948 mRNA. Translation: AAQ89307.1.
AK074618 mRNA. Translation: BAC11092.1.
AK074770 mRNA. Translation: BAC11196.1.
AC092535 Genomic DNA. Translation: AAY40988.1.
CH471131 Genomic DNA. Translation: EAW82604.1.
CH471131 Genomic DNA. Translation: EAW82606.1.
BC002707 mRNA. Translation: AAH02707.1.
BC036341 mRNA. Translation: AAH36341.1.
CCDSiCCDS3347.1.
RefSeqiNP_001121797.1. NM_001128325.2.
NP_001185950.1. NM_001199021.1.
NP_036577.1. NM_012445.3.
UniGeneiHs.302963.
Hs.635350.
Hs.736956.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D34X-ray1.80A/B27-249[»]
ProteinModelPortaliQ9BUD6.
SMRiQ9BUD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115686. 1 interactor.
IntActiQ9BUD6. 1 interactor.
STRINGi9606.ENSP00000290902.

PTM databases

iPTMnetiQ9BUD6.
PhosphoSitePlusiQ9BUD6.

Polymorphism and mutation databases

BioMutaiSPON2.
DMDMi313104285.

Proteomic databases

MaxQBiQ9BUD6.
PaxDbiQ9BUD6.
PeptideAtlasiQ9BUD6.
PRIDEiQ9BUD6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290902; ENSP00000290902; ENSG00000159674.
ENST00000431380; ENSP00000394832; ENSG00000159674.
ENST00000617421; ENSP00000483599; ENSG00000159674.
GeneIDi10417.
KEGGihsa:10417.
UCSCiuc003gco.5. human.

Organism-specific databases

CTDi10417.
DisGeNETi10417.
GeneCardsiSPON2.
HGNCiHGNC:11253. SPON2.
HPAiHPA040170.
HPA043890.
HPA066095.
MIMi605918. gene.
neXtProtiNX_Q9BUD6.
PharmGKBiPA36083.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IR0P. Eukaryota.
ENOG410XX1S. LUCA.
HOGENOMiHOG000114626.
HOVERGENiHBG052919.
InParanoidiQ9BUD6.
OrthoDBiEOG091G0BT5.
PhylomeDBiQ9BUD6.
TreeFamiTF326913.

Enzyme and pathway databases

ReactomeiR-HSA-5083635. Defective B3GALTL causes Peters-plus syndrome (PpS).
R-HSA-5173214. O-glycosylation of TSR domain-containing proteins.

Miscellaneous databases

EvolutionaryTraceiQ9BUD6.
GenomeRNAii10417.
PROiQ9BUD6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000159674.
CleanExiHS_SPON2.
ExpressionAtlasiQ9BUD6. baseline and differential.
GenevisibleiQ9BUD6. HS.

Family and domain databases

InterProiIPR009465. Spondin_N.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF06468. Spond_N. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
SMARTiSM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF82895. SSF82895. 1 hit.
PROSITEiPS51020. SPONDIN. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPON2_HUMAN
AccessioniPrimary (citable) accession number: Q9BUD6
Secondary accession number(s): D3DVN9, Q4W5N4, Q9ULW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: November 30, 2010
Last modified: November 30, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.