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Q9BUD6 (SPON2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spondin-2
Alternative name(s):
Differentially expressed in cancerous and non-cancerous lung cells 1
Short name=DIL-1
Mindin
Gene names
Name:SPON2
Synonyms:DIL1
ORF Names:UNQ435/PRO866
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens By similarity. Binds bacterial lipopolysaccharide (LPS).

Subunit structure

Monomer. Interacts with integrin. Ref.7

Subcellular location

Secretedextracellular spaceextracellular matrix By similarity.

Tissue specificity

Expressed in normal lung tissue but not in lung carcinoma cell lines. Ref.1

Sequence similarities

Contains 1 spondin domain.

Contains 1 TSP type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 331305Spondin-2
PRO_0000035870

Regions

Domain31 – 221191Spondin
Domain277 – 33155TSP type-1

Sites

Metal binding421Divalent metal cation Potential
Metal binding1221Divalent metal cation Potential
Metal binding1411Divalent metal cation Probable
Metal binding1601Calcium
Metal binding1881Calcium
Metal binding1921Calcium; via carbonyl oxygen
Site1411Important for metal ion-dependent interaction with integrin Probable

Amino acid modifications

Glycosylation2831C-linked (Man) Ref.7
Disulfide bond35 ↔ 171 Ref.7

Natural variations

Natural variant381R → G.
Corresponds to variant rs6836335 [ dbSNP | Ensembl ].
VAR_055149
Natural variant401L → P. Ref.1 Ref.2 Ref.3 Ref.5 Ref.6
Corresponds to variant rs922697 [ dbSNP | Ensembl ].
VAR_019701
Natural variant1221E → A. Ref.1 Ref.3 Ref.4 Ref.5
Corresponds to variant rs11247975 [ dbSNP | Ensembl ].
VAR_019702
Natural variant2421V → L. Ref.2 Ref.3 Ref.5 Ref.6
Corresponds to variant rs2279279 [ dbSNP | Ensembl ].
VAR_019703

Experimental info

Mutagenesis1221E → A: Strongly reduced metal-dependent interaction with integrin; when associated with A-141. Ref.7
Mutagenesis1411E → A: Strongly reduced metal-dependent interaction with integrin; when associated with A-122. Ref.7

Secondary structure

................................. 331
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9BUD6 [UniParc].

Last modified November 30, 2010. Version 3.
Checksum: 8AF827142D921705

FASTA33135,846
        10         20         30         40         50         60 
MENPSPAAAL GKALCALLLA TLGAAGQPLG GESICSARAL AKYSITFTGK WSQTAFPKQY 

        70         80         90        100        110        120 
PLFRPPAQWS SLLGAAHSSD YSMWRKNQYV SNGLRDFAER GEAWALMKEI EAAGEALQSV 

       130        140        150        160        170        180 
HEVFSAPAVP SGTGQTSAEL EVQRRHSLVS FVVRIVPSPD WFVGVDSLDL CDGDRWREQA 

       190        200        210        220        230        240 
ALDLYPYDAG TDSGFTFSSP NFATIPQDTV TEITSSSPSH PANSFYYPRL KALPPIARVT 

       250        260        270        280        290        300 
LVRLRQSPRA FIPPAPVLPS RDNEIVDSAS VPETPLDCEV SLWSSWGLCG GHCGRLGTKS 

       310        320        330 
RTRYVRVQPA NNGSPCPELE EEAECVPDNC V 

« Hide

References

« Hide 'large scale' references
[1]"Identification of genes (SPON2 and C20orf2) differentially expressed between cancerous and noncancerous lung cells by mRNA differential display."
Manda R., Kohno T., Matsuno Y., Takenoshita S., Kuwano H., Yokota J.
Genomics 61:5-14(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANTS PRO-40 AND ALA-122.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS PRO-40 AND LEU-242.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS PRO-40; ALA-122 AND LEU-242.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-122.
[5]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS PRO-40; ALA-122 AND LEU-242.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS PRO-40 AND LEU-242.
Tissue: Colon and Placenta.
[7]"Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule."
Li Y., Cao C., Jia W., Yu L., Mo M., Wang Q., Huang Y., Lim J.-M., Ishihara M., Wells L., Azadi P., Robinson H., He Y.-W., Zhang L., Mariuzza R.A.
EMBO J. 28:286-297(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 27-249 IN COMPLEX WITH METAL IONS, PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, GLYCOSYLATION AT TRP-283, INTERACTION WITH INTEGRIN AND BACTERIAL LIPOPOLYSACCHARIDE, MUTAGENESIS OF GLU-122 AND GLU-141, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB027466 mRNA. Translation: BAA85892.1.
AY358948 mRNA. Translation: AAQ89307.1.
AK074618 mRNA. Translation: BAC11092.1.
AK074770 mRNA. Translation: BAC11196.1.
AC092535 Genomic DNA. Translation: AAY40988.1.
CH471131 Genomic DNA. Translation: EAW82604.1.
CH471131 Genomic DNA. Translation: EAW82606.1.
BC002707 mRNA. Translation: AAH02707.1.
BC036341 mRNA. Translation: AAH36341.1.
CCDSCCDS3347.1.
RefSeqNP_001121797.1. NM_001128325.2.
NP_001185950.1. NM_001199021.1.
NP_036577.1. NM_012445.3.
UniGeneHs.302963.
Hs.635350.
Hs.736956.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3D34X-ray1.80A/B27-249[»]
ProteinModelPortalQ9BUD6.
SMRQ9BUD6. Positions 34-248.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000290902.

PTM databases

PhosphoSiteQ9BUD6.

Polymorphism databases

DMDM313104285.

Proteomic databases

MaxQBQ9BUD6.
PaxDbQ9BUD6.
PRIDEQ9BUD6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000290902; ENSP00000290902; ENSG00000159674.
ENST00000431380; ENSP00000394832; ENSG00000159674.
GeneID10417.
KEGGhsa:10417.

Organism-specific databases

CTD10417.
GeneCardsGC04M001150.
HGNCHGNC:11253. SPON2.
HPAHPA040170.
HPA043890.
MIM605918. gene.
neXtProtNX_Q9BUD6.
PharmGKBPA36083.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG279286.
HOGENOMHOG000114626.
HOVERGENHBG052919.
InParanoidQ9BUD6.
OrthoDBEOG71CFK9.
PhylomeDBQ9BUD6.
TreeFamTF326913.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.

Gene expression databases

ArrayExpressQ9BUD6.
BgeeQ9BUD6.
CleanExHS_SPON2.
GenevestigatorQ9BUD6.

Family and domain databases

InterProIPR009465. Spondin_N.
IPR000884. Thrombospondin_1_rpt.
[Graphical view]
PfamPF06468. Spond_N. 1 hit.
[Graphical view]
SMARTSM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMSSF82895. SSF82895. 1 hit.
PROSITEPS51020. SPONDIN. 1 hit.
PS50092. TSP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9BUD6.
GenomeRNAi10417.
NextBio39478.
PROQ9BUD6.
SOURCESearch...

Entry information

Entry nameSPON2_HUMAN
AccessionPrimary (citable) accession number: Q9BUD6
Secondary accession number(s): D3DVN9, Q4W5N4, Q9ULW1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: November 30, 2010
Last modified: July 9, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM