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Protein

Spondin-2

Gene

SPON2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Divalent metal cationSequence analysis1
Metal bindingi122Divalent metal cationSequence analysis1
Metal bindingi141Divalent metal cationCurated1
Sitei141Important for metal ion-dependent interaction with integrin1 Publication1
Metal bindingi160CalciumCombined sources1 Publication1
Metal bindingi188CalciumCombined sources1 Publication1
Metal bindingi192Calcium; via carbonyl oxygenCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell adhesion, Immunity, Innate immunity
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-5083635 Defective B3GALTL causes Peters-plus syndrome (PpS)
R-HSA-5173214 O-glycosylation of TSR domain-containing proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Spondin-2
Alternative name(s):
Differentially expressed in cancerous and non-cancerous lung cells 1
Short name:
DIL-1
Mindin
Gene namesi
Name:SPON2
Synonyms:DIL1
ORF Names:UNQ435/PRO866
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000159674.11
HGNCiHGNC:11253 SPON2
MIMi605918 gene
neXtProtiNX_Q9BUD6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi122E → A: Strongly reduced metal-dependent interaction with integrin; when associated with A-141. 1 Publication1
Mutagenesisi141E → A: Strongly reduced metal-dependent interaction with integrin; when associated with A-122. 1 Publication1

Organism-specific databases

DisGeNETi10417
PharmGKBiPA36083

Polymorphism and mutation databases

BioMutaiSPON2
DMDMi313104285

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000003587027 – 331Spondin-2Add BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi35 ↔ 171PROSITE-ProRule annotation1 Publication
Glycosylationi283C-linked (Man) tryptophan1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9BUD6
PaxDbiQ9BUD6
PeptideAtlasiQ9BUD6
PRIDEiQ9BUD6

PTM databases

iPTMnetiQ9BUD6
PhosphoSitePlusiQ9BUD6

Expressioni

Tissue specificityi

Expressed in normal lung tissue but not in lung carcinoma cell lines.1 Publication

Gene expression databases

BgeeiENSG00000159674
CleanExiHS_SPON2
ExpressionAtlasiQ9BUD6 baseline and differential
GenevisibleiQ9BUD6 HS

Organism-specific databases

HPAiHPA040170
HPA066095

Interactioni

Subunit structurei

Monomer. Interacts with integrin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT31Q153235EBI-10298801,EBI-948001

Protein-protein interaction databases

BioGridi115686, 1 interactor
IntActiQ9BUD6, 1 interactor
STRINGi9606.ENSP00000290902

Structurei

Secondary structure

1331
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 49Combined sources9
Turni53 – 55Combined sources3
Beta strandi63 – 65Combined sources3
Beta strandi67 – 77Combined sources11
Helixi92 – 100Combined sources9
Helixi104 – 117Combined sources14
Beta strandi120 – 126Combined sources7
Beta strandi129 – 131Combined sources3
Beta strandi135 – 143Combined sources9
Beta strandi148 – 157Combined sources10
Beta strandi159 – 169Combined sources11
Beta strandi178 – 184Combined sources7
Beta strandi190 – 192Combined sources3
Beta strandi215 – 218Combined sources4
Beta strandi230 – 232Combined sources3
Beta strandi236 – 243Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D34X-ray1.80A/B27-249[»]
ProteinModelPortaliQ9BUD6
SMRiQ9BUD6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BUD6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 221SpondinPROSITE-ProRule annotationAdd BLAST191
Domaini277 – 331TSP type-1PROSITE-ProRule annotationAdd BLAST55

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IR0P Eukaryota
ENOG410XX1S LUCA
HOGENOMiHOG000114626
HOVERGENiHBG052919
InParanoidiQ9BUD6
OrthoDBiEOG091G0BT5
PhylomeDBiQ9BUD6
TreeFamiTF326913

Family and domain databases

Gene3Di2.20.100.10, 1 hit
2.60.40.2130, 1 hit
InterProiView protein in InterPro
IPR009465 Spondin_N
IPR038678 Spondin_N_sf
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
PfamiView protein in Pfam
PF06468 Spond_N, 1 hit
PF00090 TSP_1, 1 hit
SMARTiView protein in SMART
SM00209 TSP1, 1 hit
SUPFAMiSSF82895 SSF82895, 1 hit
PROSITEiView protein in PROSITE
PS51020 SPONDIN, 1 hit
PS50092 TSP1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BUD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENPSPAAAL GKALCALLLA TLGAAGQPLG GESICSARAL AKYSITFTGK
60 70 80 90 100
WSQTAFPKQY PLFRPPAQWS SLLGAAHSSD YSMWRKNQYV SNGLRDFAER
110 120 130 140 150
GEAWALMKEI EAAGEALQSV HEVFSAPAVP SGTGQTSAEL EVQRRHSLVS
160 170 180 190 200
FVVRIVPSPD WFVGVDSLDL CDGDRWREQA ALDLYPYDAG TDSGFTFSSP
210 220 230 240 250
NFATIPQDTV TEITSSSPSH PANSFYYPRL KALPPIARVT LVRLRQSPRA
260 270 280 290 300
FIPPAPVLPS RDNEIVDSAS VPETPLDCEV SLWSSWGLCG GHCGRLGTKS
310 320 330
RTRYVRVQPA NNGSPCPELE EEAECVPDNC V
Length:331
Mass (Da):35,846
Last modified:November 30, 2010 - v3
Checksum:i8AF827142D921705
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05514938R → G. Corresponds to variant dbSNP:rs6836335Ensembl.1
Natural variantiVAR_01970140L → P5 PublicationsCorresponds to variant dbSNP:rs922697Ensembl.1
Natural variantiVAR_019702122E → A4 PublicationsCorresponds to variant dbSNP:rs11247975Ensembl.1
Natural variantiVAR_019703242V → L4 PublicationsCorresponds to variant dbSNP:rs2279279Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027466 mRNA Translation: BAA85892.1
AY358948 mRNA Translation: AAQ89307.1
AK074618 mRNA Translation: BAC11092.1
AK074770 mRNA Translation: BAC11196.1
AC092535 Genomic DNA Translation: AAY40988.1
CH471131 Genomic DNA Translation: EAW82604.1
CH471131 Genomic DNA Translation: EAW82606.1
BC002707 mRNA Translation: AAH02707.1
BC036341 mRNA Translation: AAH36341.1
CCDSiCCDS3347.1
RefSeqiNP_001121797.1, NM_001128325.2
NP_001185950.1, NM_001199021.1
NP_036577.1, NM_012445.3
UniGeneiHs.302963
Hs.635350
Hs.736956

Genome annotation databases

EnsembliENST00000290902; ENSP00000290902; ENSG00000159674
ENST00000431380; ENSP00000394832; ENSG00000159674
ENST00000617421; ENSP00000483599; ENSG00000159674
GeneIDi10417
KEGGihsa:10417
UCSCiuc003gco.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSPON2_HUMAN
AccessioniPrimary (citable) accession number: Q9BUD6
Secondary accession number(s): D3DVN9, Q4W5N4, Q9ULW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: November 30, 2010
Last modified: April 25, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health