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Protein

tRNA-specific adenosine deaminase 1

Gene

ADAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically deaminates adenosine-37 to inosine in tRNA-Ala.

Catalytic activityi

Adenine(37) in tRNA(Ala) + H2O = hypoxanthine(37) in tRNA(Ala) + NH3.1 Publication

Cofactori

1D-myo-inositol hexakisphosphateBy similarityNote: Binds 1 myo-inositol hexakisphosphate (IP6) per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi87 – 871ZincPROSITE-ProRule annotation
Active sitei89 – 891Proton donorPROSITE-ProRule annotation
Binding sitei93 – 931Inositol hexakisphosphateBy similarity
Binding sitei94 – 941Inositol hexakisphosphateBy similarity
Metal bindingi142 – 1421ZincPROSITE-ProRule annotation
Metal bindingi299 – 2991ZincPROSITE-ProRule annotation
Binding sitei302 – 3021Inositol hexakisphosphateBy similarity
Binding sitei305 – 3051Inositol hexakisphosphateBy similarity
Binding sitei435 – 4351Inositol hexakisphosphateBy similarity
Binding sitei470 – 4701Inositol hexakisphosphateBy similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB
  • tRNA-specific adenosine deaminase activity Source: UniProtKB

GO - Biological processi

  • tRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.4.34. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminase 1 (EC:3.5.4.34)
Short name:
hADAT1
Alternative name(s):
tRNA-specific adenosine-37 deaminase
Gene namesi
Name:ADAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:228. ADAT1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24558.

Polymorphism and mutation databases

BioMutaiADAT1.
DMDMi74733201.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502tRNA-specific adenosine deaminase 1PRO_0000287646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9BUB4.
PaxDbiQ9BUB4.
PRIDEiQ9BUB4.

PTM databases

PhosphoSiteiQ9BUB4.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiQ9BUB4.
CleanExiHS_ADAT1.
ExpressionAtlasiQ9BUB4. baseline and differential.
GenevisibleiQ9BUB4. HS.

Organism-specific databases

HPAiHPA040713.
HPA040903.
HPA061570.

Interactioni

Protein-protein interaction databases

BioGridi117081. 4 interactions.
STRINGi9606.ENSP00000310015.

Structurei

3D structure databases

ProteinModelPortaliQ9BUB4.
SMRiQ9BUB4. Positions 60-147, 290-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 501439A to I editasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ADAT1 family.Curated
Contains 1 A to I editase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG269429.
GeneTreeiENSGT00550000074412.
HOGENOMiHOG000237320.
HOVERGENiHBG059724.
InParanoidiQ9BUB4.
KOiK15440.
OMAiVVIGKCP.
PhylomeDBiQ9BUB4.
TreeFamiTF315806.

Family and domain databases

InterProiIPR002466. A_deamin.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BUB4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT
60 70 80 90 100
PDKPVQVTKE VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL
110 120 130 140 150
LHQLQLAATL KEDSIFVPGT QKGVWKLRRD LIFVFFSSHT PCGDASIIPM
160 170 180 190 200
LEFEDQPCCP VFRNWAHNSS VEASSNLEAP GNERKCEDPD SPVTKKMRLE
210 220 230 240 250
PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL ATVTRIAPGS
260 270 280 290 300
AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS
310 320 330 340 350
DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ
360 370 380 390 400
NVSALPKGFG VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW
410 420 430 440 450
SAVPEQPLDV TANGFPQGTT KKTIGSLQAR SQISKVELFR SFQKLLSRIA
460 470 480 490 500
RDKWPHSLRV QKLDTYQEYK EAASSYQEAW STLRKQVFGS WIRNPPDYHQ

FK
Length:502
Mass (Da):55,392
Last modified:June 1, 2001 - v1
Checksum:i3664E4C50DA9DFE0
GO
Isoform 2 (identifier: Q9BUB4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-149: Missing.

Note: No experimental confirmation available.
Show »
Length:353
Mass (Da):38,706
Checksum:i8251C7D7E53A9EDA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611V → I in AAD48376 (PubMed:10430867).Curated
Sequence conflicti153 – 1531F → L in BAA91837 (PubMed:14702039).Curated
Sequence conflicti254 – 2541I → T in AAD48376 (PubMed:10430867).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti167 – 1671H → N.1 Publication
Corresponds to variant rs3743598 [ dbSNP | Ensembl ].
VAR_032340
Natural varianti203 – 2031T → N.
Corresponds to variant rs3743599 [ dbSNP | Ensembl ].
VAR_032341
Natural varianti226 – 2261I → V.
Corresponds to variant rs56029288 [ dbSNP | Ensembl ].
VAR_061098
Natural varianti242 – 2421T → P.
Corresponds to variant rs3743600 [ dbSNP | Ensembl ].
VAR_055649

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 149149Missing in isoform 2. 1 PublicationVSP_025579Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125188 mRNA. Translation: AAD48376.1.
AK001687 mRNA. Translation: BAA91837.1.
BC002758 mRNA. Translation: AAH02758.1.
CCDSiCCDS10922.1. [Q9BUB4-1]
RefSeqiNP_036223.2. NM_012091.3. [Q9BUB4-1]
UniGeneiHs.729312.

Genome annotation databases

EnsembliENST00000307921; ENSP00000310015; ENSG00000065457. [Q9BUB4-1]
GeneIDi23536.
KEGGihsa:23536.
UCSCiuc002feo.2. human. [Q9BUB4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125188 mRNA. Translation: AAD48376.1.
AK001687 mRNA. Translation: BAA91837.1.
BC002758 mRNA. Translation: AAH02758.1.
CCDSiCCDS10922.1. [Q9BUB4-1]
RefSeqiNP_036223.2. NM_012091.3. [Q9BUB4-1]
UniGeneiHs.729312.

3D structure databases

ProteinModelPortaliQ9BUB4.
SMRiQ9BUB4. Positions 60-147, 290-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117081. 4 interactions.
STRINGi9606.ENSP00000310015.

PTM databases

PhosphoSiteiQ9BUB4.

Polymorphism and mutation databases

BioMutaiADAT1.
DMDMi74733201.

Proteomic databases

MaxQBiQ9BUB4.
PaxDbiQ9BUB4.
PRIDEiQ9BUB4.

Protocols and materials databases

DNASUi23536.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000307921; ENSP00000310015; ENSG00000065457. [Q9BUB4-1]
GeneIDi23536.
KEGGihsa:23536.
UCSCiuc002feo.2. human. [Q9BUB4-1]

Organism-specific databases

CTDi23536.
GeneCardsiGC16M075632.
HGNCiHGNC:228. ADAT1.
HPAiHPA040713.
HPA040903.
HPA061570.
MIMi604230. gene.
neXtProtiNX_Q9BUB4.
PharmGKBiPA24558.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG269429.
GeneTreeiENSGT00550000074412.
HOGENOMiHOG000237320.
HOVERGENiHBG059724.
InParanoidiQ9BUB4.
KOiK15440.
OMAiVVIGKCP.
PhylomeDBiQ9BUB4.
TreeFamiTF315806.

Enzyme and pathway databases

BRENDAi3.5.4.34. 2681.

Miscellaneous databases

GenomeRNAii23536.
NextBioi46034.
PROiQ9BUB4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BUB4.
CleanExiHS_ADAT1.
ExpressionAtlasiQ9BUB4. baseline and differential.
GenevisibleiQ9BUB4. HS.

Family and domain databases

InterProiIPR002466. A_deamin.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a human tRNA-specific adenosine deaminase related to the ADAR family of pre-mRNA editing enzymes."
    Maas S., Gerber A.P., Rich A.
    Proc. Natl. Acad. Sci. U.S.A. 96:8895-8900(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY, VARIANT ASN-167.
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Teratocarcinoma.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.

Entry informationi

Entry nameiADAT1_HUMAN
AccessioniPrimary (citable) accession number: Q9BUB4
Secondary accession number(s): Q9NVB7, Q9UNG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.