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Q9BUB4

- ADAT1_HUMAN

UniProt

Q9BUB4 - ADAT1_HUMAN

Protein

tRNA-specific adenosine deaminase 1

Gene

ADAT1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Specifically deaminates adenosine-37 to inosine in tRNA-Ala.

    Catalytic activityi

    Adenine(37) in tRNA(Ala) + H2O = hypoxanthine(37) in tRNA(Ala) + NH3.1 Publication

    Cofactori

    Binds 1 inositol hexakisphosphate (IP6) per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi87 – 871ZincPROSITE-ProRule annotation
    Active sitei89 – 891Proton donorPROSITE-ProRule annotation
    Binding sitei93 – 931Inositol hexakisphosphateBy similarity
    Binding sitei94 – 941Inositol hexakisphosphateBy similarity
    Metal bindingi142 – 1421ZincPROSITE-ProRule annotation
    Metal bindingi299 – 2991ZincPROSITE-ProRule annotation
    Binding sitei302 – 3021Inositol hexakisphosphateBy similarity
    Binding sitei305 – 3051Inositol hexakisphosphateBy similarity
    Binding sitei435 – 4351Inositol hexakisphosphateBy similarity
    Binding sitei470 – 4701Inositol hexakisphosphateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB
    3. tRNA-specific adenosine deaminase activity Source: UniProtKB

    GO - Biological processi

    1. tRNA processing Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-specific adenosine deaminase 1 (EC:3.5.4.34)
    Short name:
    hADAT1
    Alternative name(s):
    tRNA-specific adenosine-37 deaminase
    Gene namesi
    Name:ADAT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:228. ADAT1.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24558.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502tRNA-specific adenosine deaminase 1PRO_0000287646Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei191 – 1911PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9BUB4.
    PaxDbiQ9BUB4.
    PRIDEiQ9BUB4.

    PTM databases

    PhosphoSiteiQ9BUB4.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ9BUB4.
    BgeeiQ9BUB4.
    CleanExiHS_ADAT1.
    GenevestigatoriQ9BUB4.

    Organism-specific databases

    HPAiHPA040713.
    HPA040903.

    Interactioni

    Protein-protein interaction databases

    BioGridi117081. 2 interactions.
    STRINGi9606.ENSP00000310015.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BUB4.
    SMRiQ9BUB4. Positions 60-147, 290-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini63 – 501439A to I editasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ADAT1 family.Curated
    Contains 1 A to I editase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG269429.
    HOGENOMiHOG000237320.
    HOVERGENiHBG059724.
    InParanoidiQ9BUB4.
    KOiK15440.
    OMAiVVIGKCP.
    PhylomeDBiQ9BUB4.
    TreeFamiTF315806.

    Family and domain databases

    InterProiIPR002466. A_deamin.
    [Graphical view]
    PfamiPF02137. A_deamin. 1 hit.
    [Graphical view]
    SMARTiSM00552. ADEAMc. 1 hit.
    [Graphical view]
    PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9BUB4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT    50
    PDKPVQVTKE VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL 100
    LHQLQLAATL KEDSIFVPGT QKGVWKLRRD LIFVFFSSHT PCGDASIIPM 150
    LEFEDQPCCP VFRNWAHNSS VEASSNLEAP GNERKCEDPD SPVTKKMRLE 200
    PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL ATVTRIAPGS 250
    AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS 300
    DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ 350
    NVSALPKGFG VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW 400
    SAVPEQPLDV TANGFPQGTT KKTIGSLQAR SQISKVELFR SFQKLLSRIA 450
    RDKWPHSLRV QKLDTYQEYK EAASSYQEAW STLRKQVFGS WIRNPPDYHQ 500
    FK 502
    Length:502
    Mass (Da):55,392
    Last modified:June 1, 2001 - v1
    Checksum:i3664E4C50DA9DFE0
    GO
    Isoform 2 (identifier: Q9BUB4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-149: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:353
    Mass (Da):38,706
    Checksum:i8251C7D7E53A9EDA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti61 – 611V → I in AAD48376. (PubMed:10430867)Curated
    Sequence conflicti153 – 1531F → L in BAA91837. (PubMed:14702039)Curated
    Sequence conflicti254 – 2541I → T in AAD48376. (PubMed:10430867)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti167 – 1671H → N.1 Publication
    Corresponds to variant rs3743598 [ dbSNP | Ensembl ].
    VAR_032340
    Natural varianti203 – 2031T → N.
    Corresponds to variant rs3743599 [ dbSNP | Ensembl ].
    VAR_032341
    Natural varianti226 – 2261I → V.
    Corresponds to variant rs56029288 [ dbSNP | Ensembl ].
    VAR_061098
    Natural varianti242 – 2421T → P.
    Corresponds to variant rs3743600 [ dbSNP | Ensembl ].
    VAR_055649

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 149149Missing in isoform 2. 1 PublicationVSP_025579Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF125188 mRNA. Translation: AAD48376.1.
    AK001687 mRNA. Translation: BAA91837.1.
    BC002758 mRNA. Translation: AAH02758.1.
    CCDSiCCDS10922.1. [Q9BUB4-1]
    RefSeqiNP_036223.2. NM_012091.3. [Q9BUB4-1]
    UniGeneiHs.729312.

    Genome annotation databases

    EnsembliENST00000307921; ENSP00000310015; ENSG00000065457. [Q9BUB4-1]
    GeneIDi23536.
    KEGGihsa:23536.
    UCSCiuc002feo.2. human. [Q9BUB4-1]

    Polymorphism databases

    DMDMi74733201.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF125188 mRNA. Translation: AAD48376.1 .
    AK001687 mRNA. Translation: BAA91837.1 .
    BC002758 mRNA. Translation: AAH02758.1 .
    CCDSi CCDS10922.1. [Q9BUB4-1 ]
    RefSeqi NP_036223.2. NM_012091.3. [Q9BUB4-1 ]
    UniGenei Hs.729312.

    3D structure databases

    ProteinModelPortali Q9BUB4.
    SMRi Q9BUB4. Positions 60-147, 290-501.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117081. 2 interactions.
    STRINGi 9606.ENSP00000310015.

    PTM databases

    PhosphoSitei Q9BUB4.

    Polymorphism databases

    DMDMi 74733201.

    Proteomic databases

    MaxQBi Q9BUB4.
    PaxDbi Q9BUB4.
    PRIDEi Q9BUB4.

    Protocols and materials databases

    DNASUi 23536.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000307921 ; ENSP00000310015 ; ENSG00000065457 . [Q9BUB4-1 ]
    GeneIDi 23536.
    KEGGi hsa:23536.
    UCSCi uc002feo.2. human. [Q9BUB4-1 ]

    Organism-specific databases

    CTDi 23536.
    GeneCardsi GC16M075632.
    HGNCi HGNC:228. ADAT1.
    HPAi HPA040713.
    HPA040903.
    MIMi 604230. gene.
    neXtProti NX_Q9BUB4.
    PharmGKBi PA24558.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG269429.
    HOGENOMi HOG000237320.
    HOVERGENi HBG059724.
    InParanoidi Q9BUB4.
    KOi K15440.
    OMAi VVIGKCP.
    PhylomeDBi Q9BUB4.
    TreeFami TF315806.

    Miscellaneous databases

    GenomeRNAii 23536.
    NextBioi 46034.
    PROi Q9BUB4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BUB4.
    Bgeei Q9BUB4.
    CleanExi HS_ADAT1.
    Genevestigatori Q9BUB4.

    Family and domain databases

    InterProi IPR002466. A_deamin.
    [Graphical view ]
    Pfami PF02137. A_deamin. 1 hit.
    [Graphical view ]
    SMARTi SM00552. ADEAMc. 1 hit.
    [Graphical view ]
    PROSITEi PS50141. A_DEAMIN_EDITASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a human tRNA-specific adenosine deaminase related to the ADAR family of pre-mRNA editing enzymes."
      Maas S., Gerber A.P., Rich A.
      Proc. Natl. Acad. Sci. U.S.A. 96:8895-8900(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY, VARIANT ASN-167.
      Tissue: Brain.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Teratocarcinoma.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Uterus.

    Entry informationi

    Entry nameiADAT1_HUMAN
    AccessioniPrimary (citable) accession number: Q9BUB4
    Secondary accession number(s): Q9NVB7, Q9UNG3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 15, 2007
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3