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Protein

tRNA-specific adenosine deaminase 1

Gene

ADAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically deaminates adenosine-37 to inosine in tRNA-Ala.

Catalytic activityi

Adenine(37) in tRNA(Ala) + H2O = hypoxanthine(37) in tRNA(Ala) + NH3.1 Publication

Cofactori

1D-myo-inositol hexakisphosphateBy similarityNote: Binds 1 myo-inositol hexakisphosphate (IP6) per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi87ZincPROSITE-ProRule annotation1
Active sitei89Proton donorPROSITE-ProRule annotation1
Binding sitei93Inositol hexakisphosphateBy similarity1
Binding sitei94Inositol hexakisphosphateBy similarity1
Metal bindingi142ZincPROSITE-ProRule annotation1
Metal bindingi299ZincPROSITE-ProRule annotation1
Binding sitei302Inositol hexakisphosphateBy similarity1
Binding sitei305Inositol hexakisphosphateBy similarity1
Binding sitei435Inositol hexakisphosphateBy similarity1
Binding sitei470Inositol hexakisphosphateBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB
  • tRNA-specific adenosine deaminase activity Source: UniProtKB

GO - Biological processi

  • tRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS00841-MONOMER.
BRENDAi3.5.4.34. 2681.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminase 1 (EC:3.5.4.34)
Short name:
hADAT1
Alternative name(s):
tRNA-specific adenosine-37 deaminase
Gene namesi
Name:ADAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:228. ADAT1.

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000065457.
PharmGKBiPA24558.

Polymorphism and mutation databases

BioMutaiADAT1.
DMDMi74733201.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002876461 – 502tRNA-specific adenosine deaminase 1Add BLAST502

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BUB4.
MaxQBiQ9BUB4.
PaxDbiQ9BUB4.
PeptideAtlasiQ9BUB4.
PRIDEiQ9BUB4.

PTM databases

iPTMnetiQ9BUB4.
PhosphoSitePlusiQ9BUB4.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000065457.
CleanExiHS_ADAT1.
ExpressionAtlasiQ9BUB4. baseline and differential.

Organism-specific databases

HPAiHPA040713.
HPA040903.
HPA061570.

Interactioni

Protein-protein interaction databases

BioGridi117081. 3 interactors.
STRINGi9606.ENSP00000310015.

Structurei

3D structure databases

ProteinModelPortaliQ9BUB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 501A to I editasePROSITE-ProRule annotationAdd BLAST439

Sequence similaritiesi

Belongs to the ADAT1 family.Curated
Contains 1 A to I editase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2777. Eukaryota.
ENOG410XT0Z. LUCA.
GeneTreeiENSGT00550000074412.
HOGENOMiHOG000237320.
HOVERGENiHBG059724.
InParanoidiQ9BUB4.
KOiK15440.
OMAiMRKNGDI.
OrthoDBiEOG091G0HDD.
PhylomeDBiQ9BUB4.
TreeFamiTF315806.

Family and domain databases

InterProiIPR002466. A_deamin.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BUB4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT
60 70 80 90 100
PDKPVQVTKE VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL
110 120 130 140 150
LHQLQLAATL KEDSIFVPGT QKGVWKLRRD LIFVFFSSHT PCGDASIIPM
160 170 180 190 200
LEFEDQPCCP VFRNWAHNSS VEASSNLEAP GNERKCEDPD SPVTKKMRLE
210 220 230 240 250
PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL ATVTRIAPGS
260 270 280 290 300
AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS
310 320 330 340 350
DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ
360 370 380 390 400
NVSALPKGFG VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW
410 420 430 440 450
SAVPEQPLDV TANGFPQGTT KKTIGSLQAR SQISKVELFR SFQKLLSRIA
460 470 480 490 500
RDKWPHSLRV QKLDTYQEYK EAASSYQEAW STLRKQVFGS WIRNPPDYHQ

FK
Length:502
Mass (Da):55,392
Last modified:June 1, 2001 - v1
Checksum:i3664E4C50DA9DFE0
GO
Isoform 2 (identifier: Q9BUB4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-149: Missing.

Note: No experimental confirmation available.
Show »
Length:353
Mass (Da):38,706
Checksum:i8251C7D7E53A9EDA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61V → I in AAD48376 (PubMed:10430867).Curated1
Sequence conflicti153F → L in BAA91837 (PubMed:14702039).Curated1
Sequence conflicti254I → T in AAD48376 (PubMed:10430867).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032340167H → N.1 PublicationCorresponds to variant rs3743598dbSNPEnsembl.1
Natural variantiVAR_032341203T → N.Corresponds to variant rs3743599dbSNPEnsembl.1
Natural variantiVAR_061098226I → V.Corresponds to variant rs56029288dbSNPEnsembl.1
Natural variantiVAR_055649242T → P.Corresponds to variant rs3743600dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0255791 – 149Missing in isoform 2. 1 PublicationAdd BLAST149

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125188 mRNA. Translation: AAD48376.1.
AK001687 mRNA. Translation: BAA91837.1.
BC002758 mRNA. Translation: AAH02758.1.
CCDSiCCDS10922.1. [Q9BUB4-1]
RefSeqiNP_001311373.1. NM_001324444.1. [Q9BUB4-2]
NP_001311374.1. NM_001324445.1. [Q9BUB4-1]
NP_001311375.1. NM_001324446.1. [Q9BUB4-2]
NP_036223.2. NM_012091.4. [Q9BUB4-1]
UniGeneiHs.729312.

Genome annotation databases

EnsembliENST00000307921; ENSP00000310015; ENSG00000065457. [Q9BUB4-1]
GeneIDi23536.
KEGGihsa:23536.
UCSCiuc002feo.3. human. [Q9BUB4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125188 mRNA. Translation: AAD48376.1.
AK001687 mRNA. Translation: BAA91837.1.
BC002758 mRNA. Translation: AAH02758.1.
CCDSiCCDS10922.1. [Q9BUB4-1]
RefSeqiNP_001311373.1. NM_001324444.1. [Q9BUB4-2]
NP_001311374.1. NM_001324445.1. [Q9BUB4-1]
NP_001311375.1. NM_001324446.1. [Q9BUB4-2]
NP_036223.2. NM_012091.4. [Q9BUB4-1]
UniGeneiHs.729312.

3D structure databases

ProteinModelPortaliQ9BUB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117081. 3 interactors.
STRINGi9606.ENSP00000310015.

PTM databases

iPTMnetiQ9BUB4.
PhosphoSitePlusiQ9BUB4.

Polymorphism and mutation databases

BioMutaiADAT1.
DMDMi74733201.

Proteomic databases

EPDiQ9BUB4.
MaxQBiQ9BUB4.
PaxDbiQ9BUB4.
PeptideAtlasiQ9BUB4.
PRIDEiQ9BUB4.

Protocols and materials databases

DNASUi23536.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000307921; ENSP00000310015; ENSG00000065457. [Q9BUB4-1]
GeneIDi23536.
KEGGihsa:23536.
UCSCiuc002feo.3. human. [Q9BUB4-1]

Organism-specific databases

CTDi23536.
GeneCardsiADAT1.
HGNCiHGNC:228. ADAT1.
HPAiHPA040713.
HPA040903.
HPA061570.
MIMi604230. gene.
neXtProtiNX_Q9BUB4.
OpenTargetsiENSG00000065457.
PharmGKBiPA24558.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2777. Eukaryota.
ENOG410XT0Z. LUCA.
GeneTreeiENSGT00550000074412.
HOGENOMiHOG000237320.
HOVERGENiHBG059724.
InParanoidiQ9BUB4.
KOiK15440.
OMAiMRKNGDI.
OrthoDBiEOG091G0HDD.
PhylomeDBiQ9BUB4.
TreeFamiTF315806.

Enzyme and pathway databases

BioCyciZFISH:HS00841-MONOMER.
BRENDAi3.5.4.34. 2681.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.

Miscellaneous databases

GenomeRNAii23536.
PROiQ9BUB4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000065457.
CleanExiHS_ADAT1.
ExpressionAtlasiQ9BUB4. baseline and differential.

Family and domain databases

InterProiIPR002466. A_deamin.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADAT1_HUMAN
AccessioniPrimary (citable) accession number: Q9BUB4
Secondary accession number(s): Q9NVB7, Q9UNG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.