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Q9BUB4 (ADAT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-specific adenosine deaminase 1

Short name=hADAT1
EC=3.5.4.34
Alternative name(s):
tRNA-specific adenosine-37 deaminase
Gene names
Name:ADAT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically deaminates adenosine-37 to inosine in tRNA-Ala.

Catalytic activity

Adenine37 in tRNA(Ala) + H2O = hypoxanthine37 in tRNA(Ala) + NH3. Ref.1

Cofactor

Binds 1 inositol hexakisphosphate (IP6) per subunit By similarity.

Tissue specificity

Ubiquitously expressed. Ref.1

Sequence similarities

Belongs to the ADAT1 family.

Contains 1 A to I editase domain.

Ontologies

Keywords
   Biological processtRNA processing
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA processing

Inferred from mutant phenotype Ref.1. Source: UniProtKB

   Molecular_functionRNA binding

Inferred from mutant phenotype Ref.1. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA-specific adenosine deaminase activity

Inferred from mutant phenotype Ref.1. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BUB4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BUB4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-149: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502tRNA-specific adenosine deaminase 1
PRO_0000287646

Regions

Domain63 – 501439A to I editase

Sites

Active site891Proton donor By similarity
Metal binding871Zinc By similarity
Metal binding1421Zinc By similarity
Metal binding2991Zinc By similarity
Binding site931Inositol hexakisphosphate By similarity
Binding site941Inositol hexakisphosphate By similarity
Binding site3021Inositol hexakisphosphate By similarity
Binding site3051Inositol hexakisphosphate By similarity
Binding site4351Inositol hexakisphosphate By similarity
Binding site4701Inositol hexakisphosphate By similarity

Amino acid modifications

Modified residue1911Phosphoserine By similarity

Natural variations

Alternative sequence1 – 149149Missing in isoform 2.
VSP_025579
Natural variant1671H → N. Ref.1
Corresponds to variant rs3743598 [ dbSNP | Ensembl ].
VAR_032340
Natural variant2031T → N.
Corresponds to variant rs3743599 [ dbSNP | Ensembl ].
VAR_032341
Natural variant2261I → V.
Corresponds to variant rs56029288 [ dbSNP | Ensembl ].
VAR_061098
Natural variant2421T → P.
Corresponds to variant rs3743600 [ dbSNP | Ensembl ].
VAR_055649

Experimental info

Sequence conflict611V → I in AAD48376. Ref.1
Sequence conflict1531F → L in BAA91837. Ref.2
Sequence conflict2541I → T in AAD48376. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 3664E4C50DA9DFE0

FASTA50255,392
        10         20         30         40         50         60 
MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT PDKPVQVTKE 

        70         80         90        100        110        120 
VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL LHQLQLAATL KEDSIFVPGT 

       130        140        150        160        170        180 
QKGVWKLRRD LIFVFFSSHT PCGDASIIPM LEFEDQPCCP VFRNWAHNSS VEASSNLEAP 

       190        200        210        220        230        240 
GNERKCEDPD SPVTKKMRLE PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL 

       250        260        270        280        290        300 
ATVTRIAPGS AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS 

       310        320        330        340        350        360 
DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ NVSALPKGFG 

       370        380        390        400        410        420 
VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW SAVPEQPLDV TANGFPQGTT 

       430        440        450        460        470        480 
KKTIGSLQAR SQISKVELFR SFQKLLSRIA RDKWPHSLRV QKLDTYQEYK EAASSYQEAW 

       490        500 
STLRKQVFGS WIRNPPDYHQ FK 

« Hide

Isoform 2 [UniParc].

Checksum: 8251C7D7E53A9EDA
Show »

FASTA35338,706

References

« Hide 'large scale' references
[1]"Identification and characterization of a human tRNA-specific adenosine deaminase related to the ADAR family of pre-mRNA editing enzymes."
Maas S., Gerber A.P., Rich A.
Proc. Natl. Acad. Sci. U.S.A. 96:8895-8900(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY, VARIANT ASN-167.
Tissue: Brain.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Teratocarcinoma.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Uterus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF125188 mRNA. Translation: AAD48376.1.
AK001687 mRNA. Translation: BAA91837.1.
BC002758 mRNA. Translation: AAH02758.1.
CCDSCCDS10922.1. [Q9BUB4-1]
RefSeqNP_036223.2. NM_012091.3. [Q9BUB4-1]
UniGeneHs.729312.

3D structure databases

ProteinModelPortalQ9BUB4.
SMRQ9BUB4. Positions 60-147, 290-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117081. 2 interactions.
STRING9606.ENSP00000310015.

PTM databases

PhosphoSiteQ9BUB4.

Polymorphism databases

DMDM74733201.

Proteomic databases

MaxQBQ9BUB4.
PaxDbQ9BUB4.
PRIDEQ9BUB4.

Protocols and materials databases

DNASU23536.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000307921; ENSP00000310015; ENSG00000065457. [Q9BUB4-1]
GeneID23536.
KEGGhsa:23536.
UCSCuc002feo.2. human. [Q9BUB4-1]

Organism-specific databases

CTD23536.
GeneCardsGC16M075632.
HGNCHGNC:228. ADAT1.
HPAHPA040713.
HPA040903.
MIM604230. gene.
neXtProtNX_Q9BUB4.
PharmGKBPA24558.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG269429.
HOGENOMHOG000237320.
HOVERGENHBG059724.
InParanoidQ9BUB4.
KOK15440.
OMAVVIGKCP.
PhylomeDBQ9BUB4.
TreeFamTF315806.

Gene expression databases

ArrayExpressQ9BUB4.
BgeeQ9BUB4.
CleanExHS_ADAT1.
GenevestigatorQ9BUB4.

Family and domain databases

InterProIPR002466. A_deamin.
[Graphical view]
PfamPF02137. A_deamin. 1 hit.
[Graphical view]
SMARTSM00552. ADEAMc. 1 hit.
[Graphical view]
PROSITEPS50141. A_DEAMIN_EDITASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi23536.
NextBio46034.
PROQ9BUB4.
SOURCESearch...

Entry information

Entry nameADAT1_HUMAN
AccessionPrimary (citable) accession number: Q9BUB4
Secondary accession number(s): Q9NVB7, Q9UNG3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM