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Protein

tRNA-specific adenosine deaminase 1

Gene

ADAT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically deaminates adenosine-37 to inosine in tRNA-Ala.

Caution

Catalytic activityi

Adenine(37) in tRNA(Ala) + H2O = hypoxanthine(37) in tRNA(Ala) + NH3.1 Publication

Cofactori

1D-myo-inositol hexakisphosphateBy similarityNote: Binds 1 myo-inositol hexakisphosphate (IP6) per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi87ZincPROSITE-ProRule annotation1
Active sitei89Proton donorPROSITE-ProRule annotation1
Binding sitei93Inositol hexakisphosphateBy similarity1
Binding sitei94Inositol hexakisphosphateBy similarity1
Metal bindingi142ZincPROSITE-ProRule annotation1
Metal bindingi299ZincPROSITE-ProRule annotation1
Binding sitei302Inositol hexakisphosphateBy similarity1
Binding sitei305Inositol hexakisphosphateBy similarity1
Binding sitei435Inositol hexakisphosphateBy similarity1
Binding sitei470Inositol hexakisphosphateBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB
  • tRNA-specific adenosine deaminase activity Source: UniProtKB

GO - Biological processi

  • tRNA processing Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processtRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.4.34 2681
ReactomeiR-HSA-6782315 tRNA modification in the nucleus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific adenosine deaminase 1 (EC:3.5.4.34)
Short name:
hADAT1
Alternative name(s):
tRNA-specific adenosine-37 deaminase
Gene namesi
Name:ADAT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000065457.10
HGNCiHGNC:228 ADAT1
MIMi604230 gene
neXtProtiNX_Q9BUB4

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000065457
PharmGKBiPA24558

Polymorphism and mutation databases

BioMutaiADAT1
DMDMi74733201

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002876461 – 502tRNA-specific adenosine deaminase 1Add BLAST502

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BUB4
MaxQBiQ9BUB4
PaxDbiQ9BUB4
PeptideAtlasiQ9BUB4
PRIDEiQ9BUB4

PTM databases

iPTMnetiQ9BUB4
PhosphoSitePlusiQ9BUB4

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSG00000065457
CleanExiHS_ADAT1
ExpressionAtlasiQ9BUB4 baseline and differential

Organism-specific databases

HPAiHPA040713
HPA040903

Interactioni

Protein-protein interaction databases

BioGridi1170814 interactors.
STRINGi9606.ENSP00000310015

Structurei

3D structure databases

ProteinModelPortaliQ9BUB4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 501A to I editasePROSITE-ProRule annotationAdd BLAST439

Sequence similaritiesi

Belongs to the ADAT1 family.Curated

Phylogenomic databases

eggNOGiKOG2777 Eukaryota
ENOG410XT0Z LUCA
GeneTreeiENSGT00550000074412
HOGENOMiHOG000237320
HOVERGENiHBG059724
InParanoidiQ9BUB4
KOiK15440
OMAiYLLHQLH
OrthoDBiEOG091G0HDD
PhylomeDBiQ9BUB4
TreeFamiTF315806

Family and domain databases

InterProiView protein in InterPro
IPR002466 A_deamin
PfamiView protein in Pfam
PF02137 A_deamin, 1 hit
SMARTiView protein in SMART
SM00552 ADEAMc, 1 hit
PROSITEiView protein in PROSITE
PS50141 A_DEAMIN_EDITASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BUB4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWTADEIAQL CYEHYGIRLP KKGKPEPNHE WTLLAAVVKI QSPADKACDT
60 70 80 90 100
PDKPVQVTKE VVSMGTGTKC IGQSKMRKNG DILNDSHAEV IARRSFQRYL
110 120 130 140 150
LHQLQLAATL KEDSIFVPGT QKGVWKLRRD LIFVFFSSHT PCGDASIIPM
160 170 180 190 200
LEFEDQPCCP VFRNWAHNSS VEASSNLEAP GNERKCEDPD SPVTKKMRLE
210 220 230 240 250
PGTAAREVTN GAAHHQSFGK QKSGPISPGI HSCDLTVEGL ATVTRIAPGS
260 270 280 290 300
AKVIDVYRTG AKCVPGEAGD SGKPGAAFHQ VGLLRVKPGR GDRTRSMSCS
310 320 330 340 350
DKMARWNVLG CQGALLMHLL EEPIYLSAVV IGKCPYSQEA MQRALIGRCQ
360 370 380 390 400
NVSALPKGFG VQELKILQSD LLFEQSRSAV QAKRADSPGR LVPCGAAISW
410 420 430 440 450
SAVPEQPLDV TANGFPQGTT KKTIGSLQAR SQISKVELFR SFQKLLSRIA
460 470 480 490 500
RDKWPHSLRV QKLDTYQEYK EAASSYQEAW STLRKQVFGS WIRNPPDYHQ

FK
Length:502
Mass (Da):55,392
Last modified:June 1, 2001 - v1
Checksum:i3664E4C50DA9DFE0
GO
Isoform 2 (identifier: Q9BUB4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-149: Missing.

Note: No experimental confirmation available.
Show »
Length:353
Mass (Da):38,706
Checksum:i8251C7D7E53A9EDA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61V → I in AAD48376 (PubMed:10430867).Curated1
Sequence conflicti153F → L in BAA91837 (PubMed:14702039).Curated1
Sequence conflicti254I → T in AAD48376 (PubMed:10430867).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032340167H → N1 PublicationCorresponds to variant dbSNP:rs3743598Ensembl.1
Natural variantiVAR_032341203T → N. Corresponds to variant dbSNP:rs3743599Ensembl.1
Natural variantiVAR_061098226I → V. Corresponds to variant dbSNP:rs56029288Ensembl.1
Natural variantiVAR_055649242T → P. Corresponds to variant dbSNP:rs3743600Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0255791 – 149Missing in isoform 2. 1 PublicationAdd BLAST149

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125188 mRNA Translation: AAD48376.1
AK001687 mRNA Translation: BAA91837.1
BC002758 mRNA Translation: AAH02758.1
CCDSiCCDS10922.1 [Q9BUB4-1]
RefSeqiNP_001311373.1, NM_001324444.1 [Q9BUB4-2]
NP_001311374.1, NM_001324445.1 [Q9BUB4-1]
NP_001311375.1, NM_001324446.1 [Q9BUB4-2]
NP_036223.2, NM_012091.4 [Q9BUB4-1]
UniGeneiHs.729312

Genome annotation databases

EnsembliENST00000307921; ENSP00000310015; ENSG00000065457 [Q9BUB4-1]
GeneIDi23536
KEGGihsa:23536
UCSCiuc002feo.3 human [Q9BUB4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiADAT1_HUMAN
AccessioniPrimary (citable) accession number: Q9BUB4
Secondary accession number(s): Q9NVB7, Q9UNG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome