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Protein

Deoxyhypusine hydroxylase

Gene

DOHH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation2 Publications

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation1 Publication

Cofactori

Fe2+UniRule annotation2 PublicationsNote: Binds 2 Fe2+ ions per subunit.UniRule annotation2 Publications

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Iron 11
Metal bindingi57Iron 11
Metal bindingi89Iron 11
Metal bindingi90Iron 11
Metal bindingi207Iron 21
Metal bindingi208Iron 21
Metal bindingi240Iron 21
Metal bindingi241Iron 21

GO - Molecular functioni

  • deoxyhypusine monooxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processHypusine biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05318-MONOMER.
BRENDAi1.14.99.29. 2681.
ReactomeiR-HSA-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
hDOHH
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
HEAT-like repeat-containing protein 1
Gene namesi
Name:DOHHUniRule annotation
Synonyms:HLRC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000129932.7.
HGNCiHGNC:28662. DOHH.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi57E → A: Abolishes enzyme activity and impairs iron-binding. 1 Publication1
Mutagenesisi89H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi90E → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi207H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi208E → A: Abolishes enzyme activity and impairs iron-binding. 1 Publication1
Mutagenesisi240H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi241E → A: Abolishes both iron-binding and enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi83475.
OpenTargetsiENSG00000129932.
PharmGKBiPA142671678.

Polymorphism and mutation databases

BioMutaiDOHH.
DMDMi74733193.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002485751 – 302Deoxyhypusine hydroxylaseAdd BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9BU89.
MaxQBiQ9BU89.
PaxDbiQ9BU89.
PeptideAtlasiQ9BU89.
PRIDEiQ9BU89.

PTM databases

iPTMnetiQ9BU89.
PhosphoSitePlusiQ9BU89.

Expressioni

Gene expression databases

BgeeiENSG00000129932.
CleanExiHS_DOHH.
ExpressionAtlasiQ9BU89. baseline and differential.
GenevisibleiQ9BU89. HS.

Organism-specific databases

HPAiHPA041953.

Interactioni

Protein-protein interaction databases

BioGridi123662. 14 interactors.
IntActiQ9BU89. 1 interactor.
STRINGi9606.ENSP00000250937.

Structurei

Secondary structure

1302
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 15Combined sources15
Helixi21 – 34Combined sources14
Helixi37 – 45Combined sources9
Helixi46 – 48Combined sources3
Helixi52 – 65Combined sources14
Helixi68 – 70Combined sources3
Helixi71 – 79Combined sources9
Helixi85 – 98Combined sources14
Helixi101 – 103Combined sources3
Helixi104 – 110Combined sources7
Helixi116 – 135Combined sources20
Helixi158 – 166Combined sources9
Helixi172 – 185Combined sources14
Helixi187 – 196Combined sources10
Helixi197 – 199Combined sources3
Helixi203 – 216Combined sources14
Helixi219 – 221Combined sources3
Helixi222 – 230Combined sources9
Helixi236 – 249Combined sources14
Helixi252 – 261Combined sources10
Helixi267 – 282Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4D4ZX-ray1.70A/B1-289[»]
4D50X-ray1.70A/B1-289[»]
ProteinModelPortaliQ9BU89.
SMRiQ9BU89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati54 – 80HEAT-like PBS-type 1Add BLAST27
Repeati87 – 113HEAT-like PBS-type 2Add BLAST27
Repeati174 – 200HEAT-like PBS-type 3Add BLAST27
Repeati205 – 231HEAT-like PBS-type 4Add BLAST27
Repeati238 – 264HEAT-like PBS-type 5Add BLAST27

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0567. Eukaryota.
COG1413. LUCA.
GeneTreeiENSGT00500000044957.
HOGENOMiHOG000248665.
HOVERGENiHBG081460.
InParanoidiQ9BU89.
KOiK06072.
OMAiPEFQYAD.
OrthoDBiEOG091G0E2R.
PhylomeDBiQ9BU89.
TreeFamiTF105626.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase. 1 hit.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR021133. HEAT_type_2.
IPR004155. PBS_lyase_HEAT.
SMARTiView protein in SMART
SM00567. EZ_HEAT. 6 hits.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiView protein in PROSITE
PS50077. HEAT_REPEAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BU89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTEQEVDAI GQTLVDPKQP LQARFRALFT LRGLGGPGAI AWISQAFDDD
60 70 80 90 100
SALLKHELAY CLGQMQDARA IPMLVDVLQD TRQEPMVRHE AGEALGAIGD
110 120 130 140 150
PEVLEILKQY SSDPVIEVAE TCQLAVRRLE WLQQHGGEPA AGPYLSVDPA
160 170 180 190 200
PPAEERDVGR LREALLDESR PLFERYRAMF ALRNAGGEEA ALALAEGLHC
210 220 230 240 250
GSALFRHEVG YVLGQLQHEA AVPQLAAALA RCTENPMVRH ECAEALGAIA
260 270 280 290 300
RPACLAALQA HADDPERVVR ESCEVALDMY EHETGRAFQY ADGLEQLRGA

PS
Length:302
Mass (Da):32,904
Last modified:June 1, 2001 - v1
Checksum:iCDA86E5549B98A2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005551 Genomic DNA. Translation: AAC33193.1.
AC093052 Genomic DNA. No translation available.
BC002817 mRNA. Translation: AAH02817.1.
BC009863 mRNA. Translation: AAH09863.1.
CCDSiCCDS12108.1.
RefSeqiNP_001138637.1. NM_001145165.1.
NP_112594.1. NM_031304.4.
UniGeneiHs.515064.
Hs.609012.

Genome annotation databases

EnsembliENST00000427575; ENSP00000398882; ENSG00000129932.
GeneIDi83475.
KEGGihsa:83475.
UCSCiuc002lxs.4. human.

Similar proteinsi

Entry informationi

Entry nameiDOHH_HUMAN
AccessioniPrimary (citable) accession number: Q9BU89
Secondary accession number(s): O75265
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2001
Last modified: October 25, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families