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Q9BU70

- NAP1_HUMAN

UniProt

Q9BU70 - NAP1_HUMAN

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Protein

Nef-associated protein 1

Gene

C9orf156

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes acyl-CoA thioesters (in vitro). Has a preference for substrates with medium chain length (C10-C14). Inactive towards substrates with C18 or C20 aliphatic chains. Its physiological substrate is not known.1 Publication

Kineticsi

  1. KM=10.1 µM for decanoyl-CoA1 Publication

Vmax=7.1 µmol/min/mg enzyme1 Publication

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  1. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Host-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Nef-associated protein 1 (EC:3.1.2.-)
Alternative name(s):
Thioesterase NAP1
Gene namesi
Name:C9orf156
Synonyms:NAP1
ORF Names:HSPC219
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:30967. C9orf156.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134875112.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Nef-associated protein 1PRO_0000288886Add
BLAST

Proteomic databases

MaxQBiQ9BU70.
PaxDbiQ9BU70.
PRIDEiQ9BU70.

PTM databases

PhosphoSiteiQ9BU70.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiQ9BU70.
CleanExiHS_C9orf156.
ExpressionAtlasiQ9BU70. baseline and differential.
GenevestigatoriQ9BU70.

Organism-specific databases

HPAiHPA021281.
HPA052197.

Interactioni

Subunit structurei

Interacts with Nef from human immunodeficiency virus (HIV-1).1 Publication

Protein-protein interaction databases

BioGridi119593. 7 interactions.
IntActiQ9BU70. 1 interaction.
STRINGi9606.ENSP00000364260.

Structurei

3D structure databases

ProteinModelPortaliQ9BU70.
SMRiQ9BU70. Positions 28-164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 168139TsaA-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 TsaA-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1720.
GeneTreeiENSGT00390000004643.
HOGENOMiHOG000231645.
HOVERGENiHBG054288.
InParanoidiQ9BU70.
OMAiFKYFQSA.
OrthoDBiEOG7P8P7P.
PhylomeDBiQ9BU70.
TreeFamiTF331670.

Family and domain databases

Gene3Di2.40.30.70. 1 hit.
InterProiIPR023370. TsaA-like.
IPR001378. UPF0066.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamiPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMiSSF118196. SSF118196. 2 hits.
TIGRFAMsiTIGR00104. YaeB_AF0241. 1 hit.
PROSITEiPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BU70-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRGLEESGPR PTATPCGCVK PALETGNLLT EPVGYLESCF SAKNGTPRQP
60 70 80 90 100
SICSYSRACL RIRKRIFNNP EHSLMGLEQF SHVWILFVFH KNGHLSCKAK
110 120 130 140 150
VQPPRLNGAK TGVFSTRSPH RPNAIGLTLA KLEKVEGGAI YLSGIDMIHG
160 170 180 190 200
TPVLDIKPYI AEYDSPQNVM EPLADFNLQN NQHTPNTVSQ SDSKTDSCDQ
210 220 230 240 250
RQLSGCDEPQ PHHSTKRKPK CPEDRTSEEN YLTHSDTARI QQAFPMHREI
260 270 280 290 300
AVDFGLESRR DQSSSVAEEQ IGPYCPEKSF SEKGTDKKLE RVEGAAVLQG
310 320 330 340 350
SRAETQPMAP HCPAGRADGA PRSVVPAWVT EAPVATLEVR FTPHAEMDLG
360 370 380 390 400
QLSSQDVGQA SFKYFQSAEE AKRAIEAVLS ADPRSVYRRK LCQDRLFYFT
410 420 430 440
VDIAHVTCWF GDGFAEVLRI KPASEPVHMT GPVGSLVSLG S
Length:441
Mass (Da):48,587
Last modified:May 29, 2007 - v2
Checksum:i84E6CF8BD294EDD2
GO

Sequence cautioni

The sequence AAF36139.1 differs from that shown. Reason: Contaminating sequence.Curated
The sequence AAF36139.1 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti341 – 3411F → V in CAA60025. (PubMed:9153233)Curated
Sequence conflicti358 – 3581G → D in BAA91013. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti7 – 71S → P.2 Publications
Corresponds to variant rs3183927 [ dbSNP | Ensembl ].
VAR_032527
Natural varianti252 – 2521V → A.
Corresponds to variant rs35606344 [ dbSNP | Ensembl ].
VAR_032528
Natural varianti324 – 3241V → M.
Corresponds to variant rs2282192 [ dbSNP | Ensembl ].
VAR_032529

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000213 mRNA. Translation: BAA91013.1.
AL499604 Genomic DNA. Translation: CAI12805.1.
BC002863 mRNA. Translation: AAH02863.1.
AF151053 mRNA. Translation: AAF36139.1. Sequence problems.
X86033 mRNA. Translation: CAA60025.1.
CCDSiCCDS6730.1.
RefSeqiNP_057565.3. NM_016481.4.
UniGeneiHs.9196.

Genome annotation databases

EnsembliENST00000375119; ENSP00000364260; ENSG00000136932.
GeneIDi51531.
KEGGihsa:51531.
UCSCiuc004axv.1. human.

Polymorphism databases

DMDMi152112517.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000213 mRNA. Translation: BAA91013.1 .
AL499604 Genomic DNA. Translation: CAI12805.1 .
BC002863 mRNA. Translation: AAH02863.1 .
AF151053 mRNA. Translation: AAF36139.1 . Sequence problems.
X86033 mRNA. Translation: CAA60025.1 .
CCDSi CCDS6730.1.
RefSeqi NP_057565.3. NM_016481.4.
UniGenei Hs.9196.

3D structure databases

ProteinModelPortali Q9BU70.
SMRi Q9BU70. Positions 28-164.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119593. 7 interactions.
IntActi Q9BU70. 1 interaction.
STRINGi 9606.ENSP00000364260.

PTM databases

PhosphoSitei Q9BU70.

Polymorphism databases

DMDMi 152112517.

Proteomic databases

MaxQBi Q9BU70.
PaxDbi Q9BU70.
PRIDEi Q9BU70.

Protocols and materials databases

DNASUi 51531.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000375119 ; ENSP00000364260 ; ENSG00000136932 .
GeneIDi 51531.
KEGGi hsa:51531.
UCSCi uc004axv.1. human.

Organism-specific databases

CTDi 51531.
GeneCardsi GC09M100666.
H-InvDB HIX0008217.
HGNCi HGNC:30967. C9orf156.
HPAi HPA021281.
HPA052197.
neXtProti NX_Q9BU70.
PharmGKBi PA134875112.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1720.
GeneTreei ENSGT00390000004643.
HOGENOMi HOG000231645.
HOVERGENi HBG054288.
InParanoidi Q9BU70.
OMAi FKYFQSA.
OrthoDBi EOG7P8P7P.
PhylomeDBi Q9BU70.
TreeFami TF331670.

Miscellaneous databases

GenomeRNAii 51531.
NextBioi 55274.

Gene expression databases

Bgeei Q9BU70.
CleanExi HS_C9orf156.
ExpressionAtlasi Q9BU70. baseline and differential.
Genevestigatori Q9BU70.

Family and domain databases

Gene3Di 2.40.30.70. 1 hit.
InterProi IPR023370. TsaA-like.
IPR001378. UPF0066.
IPR023368. UPF0066_cons_site.
[Graphical view ]
Pfami PF01980. UPF0066. 1 hit.
[Graphical view ]
SUPFAMi SSF118196. SSF118196. 2 hits.
TIGRFAMsi TIGR00104. YaeB_AF0241. 1 hit.
PROSITEi PS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-7.
    Tissue: Colon mucosa.
  2. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-7.
    Tissue: Lung.
  4. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-241.
    Tissue: Umbilical cord blood.
  5. "Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation."
    Liu L.X., Margottin F., Le Gall S., Schwartz O., Selig L., Benarous R., Benichou S.
    J. Biol. Chem. 272:13779-13785(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 218-441, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH HIV-1 NEF, TISSUE SPECIFICITY.
    Tissue: Lymphocyte.

Entry informationi

Entry nameiNAP1_HUMAN
AccessioniPrimary (citable) accession number: Q9BU70
Secondary accession number(s): Q5T113
, Q86SK0, Q9NXJ7, Q9P0Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: October 29, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3