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Q9BU70 (NAP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nef-associated protein 1

EC=3.1.2.-
Alternative name(s):
Thioesterase NAP1
Gene names
Name:C9orf156
Synonyms:NAP1
ORF Names:HSPC219
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length441 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes acyl-CoA thioesters (in vitro). Has a preference for substrates with medium chain length (C10-C14). Inactive towards substrates with C18 or C20 aliphatic chains. Its physiological substrate is not known. Ref.5

Subunit structure

Interacts with Nef from human immunodeficiency virus (HIV-1). Ref.5

Tissue specificity

Ubiquitous. Ref.5

Sequence similarities

Contains 1 TsaA-like domain.

Biophysicochemical properties

Kinetic parameters:

KM=10.1 µM for decanoyl-CoA Ref.5

Vmax=7.1 µmol/min/mg enzyme

Sequence caution

The sequence AAF36139.1 differs from that shown. Reason: Frameshift at several positions.

The sequence AAF36139.1 differs from that shown. Reason: Contaminating sequence.

Ontologies

Keywords
   Biological processHost-virus interaction
   Coding sequence diversityPolymorphism
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processviral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionhydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 441441Nef-associated protein 1
PRO_0000288886

Regions

Domain30 – 168139TsaA-like

Natural variations

Natural variant71S → P. Ref.1 Ref.3
Corresponds to variant rs3183927 [ dbSNP | Ensembl ].
VAR_032527
Natural variant2521V → A.
Corresponds to variant rs35606344 [ dbSNP | Ensembl ].
VAR_032528
Natural variant3241V → M.
Corresponds to variant rs2282192 [ dbSNP | Ensembl ].
VAR_032529

Experimental info

Sequence conflict3411F → V in CAA60025. Ref.5
Sequence conflict3581G → D in BAA91013. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9BU70 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: 84E6CF8BD294EDD2

FASTA44148,587
        10         20         30         40         50         60 
MRGLEESGPR PTATPCGCVK PALETGNLLT EPVGYLESCF SAKNGTPRQP SICSYSRACL 

        70         80         90        100        110        120 
RIRKRIFNNP EHSLMGLEQF SHVWILFVFH KNGHLSCKAK VQPPRLNGAK TGVFSTRSPH 

       130        140        150        160        170        180 
RPNAIGLTLA KLEKVEGGAI YLSGIDMIHG TPVLDIKPYI AEYDSPQNVM EPLADFNLQN 

       190        200        210        220        230        240 
NQHTPNTVSQ SDSKTDSCDQ RQLSGCDEPQ PHHSTKRKPK CPEDRTSEEN YLTHSDTARI 

       250        260        270        280        290        300 
QQAFPMHREI AVDFGLESRR DQSSSVAEEQ IGPYCPEKSF SEKGTDKKLE RVEGAAVLQG 

       310        320        330        340        350        360 
SRAETQPMAP HCPAGRADGA PRSVVPAWVT EAPVATLEVR FTPHAEMDLG QLSSQDVGQA 

       370        380        390        400        410        420 
SFKYFQSAEE AKRAIEAVLS ADPRSVYRRK LCQDRLFYFT VDIAHVTCWF GDGFAEVLRI 

       430        440 
KPASEPVHMT GPVGSLVSLG S 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-7.
Tissue: Colon mucosa.
[2]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-7.
Tissue: Lung.
[4]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-241.
Tissue: Umbilical cord blood.
[5]"Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation."
Liu L.X., Margottin F., Le Gall S., Schwartz O., Selig L., Benarous R., Benichou S.
J. Biol. Chem. 272:13779-13785(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 218-441, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH HIV-1 NEF, TISSUE SPECIFICITY.
Tissue: Lymphocyte.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK000213 mRNA. Translation: BAA91013.1.
AL499604 Genomic DNA. Translation: CAI12805.1.
BC002863 mRNA. Translation: AAH02863.1.
AF151053 mRNA. Translation: AAF36139.1. Sequence problems.
X86033 mRNA. Translation: CAA60025.1.
RefSeqNP_057565.3. NM_016481.4.
UniGeneHs.9196.

3D structure databases

ProteinModelPortalQ9BU70.
SMRQ9BU70. Positions 28-164.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119593. 5 interactions.
IntActQ9BU70. 1 interaction.
STRING9606.ENSP00000364260.

PTM databases

PhosphoSiteQ9BU70.

Polymorphism databases

DMDM152112517.

Proteomic databases

PaxDbQ9BU70.
PRIDEQ9BU70.

Protocols and materials databases

DNASU51531.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000375119; ENSP00000364260; ENSG00000136932.
GeneID51531.
KEGGhsa:51531.
UCSCuc004axv.1. human.

Organism-specific databases

CTD51531.
GeneCardsGC09M100666.
H-InvDBHIX0008217.
HGNCHGNC:30967. C9orf156.
HPAHPA021281.
HPA052197.
neXtProtNX_Q9BU70.
PharmGKBPA134875112.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1720.
HOGENOMHOG000231645.
HOVERGENHBG054288.
InParanoidQ9BU70.
OMAFKYFQSA.
OrthoDBEOG7P8P7P.
PhylomeDBQ9BU70.
TreeFamTF331670.

Gene expression databases

ArrayExpressQ9BU70.
BgeeQ9BU70.
CleanExHS_C9orf156.
GenevestigatorQ9BU70.

Family and domain databases

Gene3D2.40.30.70. 1 hit.
InterProIPR023370. TsaA-like.
IPR001378. UPF0066.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMSSF118196. SSF118196. 2 hits.
TIGRFAMsTIGR00104. YaeB_AF0241. 1 hit.
PROSITEPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi51531.
NextBio55274.

Entry information

Entry nameNAP1_HUMAN
AccessionPrimary (citable) accession number: Q9BU70
Secondary accession number(s): Q5T113 expand/collapse secondary AC list , Q86SK0, Q9NXJ7, Q9P0Q7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM