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Q9BU70

- NAP1_HUMAN

UniProt

Q9BU70 - NAP1_HUMAN

Protein

Nef-associated protein 1

Gene

C9orf156

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (29 May 2007)
      Previous versions | rss
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    Functioni

    Hydrolyzes acyl-CoA thioesters (in vitro). Has a preference for substrates with medium chain length (C10-C14). Inactive towards substrates with C18 or C20 aliphatic chains. Its physiological substrate is not known.1 Publication

    Kineticsi

    1. KM=10.1 µM for decanoyl-CoA1 Publication

    Vmax=7.1 µmol/min/mg enzyme1 Publication

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW

    GO - Biological processi

    1. viral process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Host-virus interaction

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nef-associated protein 1 (EC:3.1.2.-)
    Alternative name(s):
    Thioesterase NAP1
    Gene namesi
    Name:C9orf156
    Synonyms:NAP1
    ORF Names:HSPC219
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:30967. C9orf156.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134875112.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 441441Nef-associated protein 1PRO_0000288886Add
    BLAST

    Proteomic databases

    MaxQBiQ9BU70.
    PaxDbiQ9BU70.
    PRIDEiQ9BU70.

    PTM databases

    PhosphoSiteiQ9BU70.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Gene expression databases

    ArrayExpressiQ9BU70.
    BgeeiQ9BU70.
    CleanExiHS_C9orf156.
    GenevestigatoriQ9BU70.

    Organism-specific databases

    HPAiHPA021281.
    HPA052197.

    Interactioni

    Subunit structurei

    Interacts with Nef from human immunodeficiency virus (HIV-1).1 Publication

    Protein-protein interaction databases

    BioGridi119593. 5 interactions.
    IntActiQ9BU70. 1 interaction.
    STRINGi9606.ENSP00000364260.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BU70.
    SMRiQ9BU70. Positions 28-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 168139TsaA-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 TsaA-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1720.
    HOGENOMiHOG000231645.
    HOVERGENiHBG054288.
    InParanoidiQ9BU70.
    OMAiFKYFQSA.
    OrthoDBiEOG7P8P7P.
    PhylomeDBiQ9BU70.
    TreeFamiTF331670.

    Family and domain databases

    Gene3Di2.40.30.70. 1 hit.
    InterProiIPR023370. TsaA-like.
    IPR001378. UPF0066.
    IPR023368. UPF0066_cons_site.
    [Graphical view]
    PfamiPF01980. UPF0066. 1 hit.
    [Graphical view]
    SUPFAMiSSF118196. SSF118196. 2 hits.
    TIGRFAMsiTIGR00104. YaeB_AF0241. 1 hit.
    PROSITEiPS01318. TSAA_1. 1 hit.
    PS51668. TSAA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9BU70-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRGLEESGPR PTATPCGCVK PALETGNLLT EPVGYLESCF SAKNGTPRQP    50
    SICSYSRACL RIRKRIFNNP EHSLMGLEQF SHVWILFVFH KNGHLSCKAK 100
    VQPPRLNGAK TGVFSTRSPH RPNAIGLTLA KLEKVEGGAI YLSGIDMIHG 150
    TPVLDIKPYI AEYDSPQNVM EPLADFNLQN NQHTPNTVSQ SDSKTDSCDQ 200
    RQLSGCDEPQ PHHSTKRKPK CPEDRTSEEN YLTHSDTARI QQAFPMHREI 250
    AVDFGLESRR DQSSSVAEEQ IGPYCPEKSF SEKGTDKKLE RVEGAAVLQG 300
    SRAETQPMAP HCPAGRADGA PRSVVPAWVT EAPVATLEVR FTPHAEMDLG 350
    QLSSQDVGQA SFKYFQSAEE AKRAIEAVLS ADPRSVYRRK LCQDRLFYFT 400
    VDIAHVTCWF GDGFAEVLRI KPASEPVHMT GPVGSLVSLG S 441
    Length:441
    Mass (Da):48,587
    Last modified:May 29, 2007 - v2
    Checksum:i84E6CF8BD294EDD2
    GO

    Sequence cautioni

    The sequence AAF36139.1 differs from that shown. Reason: Contaminating sequence.
    The sequence AAF36139.1 differs from that shown. Reason: Frameshift at several positions.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti341 – 3411F → V in CAA60025. (PubMed:9153233)Curated
    Sequence conflicti358 – 3581G → D in BAA91013. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti7 – 71S → P.2 Publications
    Corresponds to variant rs3183927 [ dbSNP | Ensembl ].
    VAR_032527
    Natural varianti252 – 2521V → A.
    Corresponds to variant rs35606344 [ dbSNP | Ensembl ].
    VAR_032528
    Natural varianti324 – 3241V → M.
    Corresponds to variant rs2282192 [ dbSNP | Ensembl ].
    VAR_032529

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK000213 mRNA. Translation: BAA91013.1.
    AL499604 Genomic DNA. Translation: CAI12805.1.
    BC002863 mRNA. Translation: AAH02863.1.
    AF151053 mRNA. Translation: AAF36139.1. Sequence problems.
    X86033 mRNA. Translation: CAA60025.1.
    CCDSiCCDS6730.1.
    RefSeqiNP_057565.3. NM_016481.4.
    UniGeneiHs.9196.

    Genome annotation databases

    EnsembliENST00000375119; ENSP00000364260; ENSG00000136932.
    GeneIDi51531.
    KEGGihsa:51531.
    UCSCiuc004axv.1. human.

    Polymorphism databases

    DMDMi152112517.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK000213 mRNA. Translation: BAA91013.1 .
    AL499604 Genomic DNA. Translation: CAI12805.1 .
    BC002863 mRNA. Translation: AAH02863.1 .
    AF151053 mRNA. Translation: AAF36139.1 . Sequence problems.
    X86033 mRNA. Translation: CAA60025.1 .
    CCDSi CCDS6730.1.
    RefSeqi NP_057565.3. NM_016481.4.
    UniGenei Hs.9196.

    3D structure databases

    ProteinModelPortali Q9BU70.
    SMRi Q9BU70. Positions 28-164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119593. 5 interactions.
    IntActi Q9BU70. 1 interaction.
    STRINGi 9606.ENSP00000364260.

    PTM databases

    PhosphoSitei Q9BU70.

    Polymorphism databases

    DMDMi 152112517.

    Proteomic databases

    MaxQBi Q9BU70.
    PaxDbi Q9BU70.
    PRIDEi Q9BU70.

    Protocols and materials databases

    DNASUi 51531.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000375119 ; ENSP00000364260 ; ENSG00000136932 .
    GeneIDi 51531.
    KEGGi hsa:51531.
    UCSCi uc004axv.1. human.

    Organism-specific databases

    CTDi 51531.
    GeneCardsi GC09M100666.
    H-InvDB HIX0008217.
    HGNCi HGNC:30967. C9orf156.
    HPAi HPA021281.
    HPA052197.
    neXtProti NX_Q9BU70.
    PharmGKBi PA134875112.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1720.
    HOGENOMi HOG000231645.
    HOVERGENi HBG054288.
    InParanoidi Q9BU70.
    OMAi FKYFQSA.
    OrthoDBi EOG7P8P7P.
    PhylomeDBi Q9BU70.
    TreeFami TF331670.

    Miscellaneous databases

    GenomeRNAii 51531.
    NextBioi 55274.

    Gene expression databases

    ArrayExpressi Q9BU70.
    Bgeei Q9BU70.
    CleanExi HS_C9orf156.
    Genevestigatori Q9BU70.

    Family and domain databases

    Gene3Di 2.40.30.70. 1 hit.
    InterProi IPR023370. TsaA-like.
    IPR001378. UPF0066.
    IPR023368. UPF0066_cons_site.
    [Graphical view ]
    Pfami PF01980. UPF0066. 1 hit.
    [Graphical view ]
    SUPFAMi SSF118196. SSF118196. 2 hits.
    TIGRFAMsi TIGR00104. YaeB_AF0241. 1 hit.
    PROSITEi PS01318. TSAA_1. 1 hit.
    PS51668. TSAA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-7.
      Tissue: Colon mucosa.
    2. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PRO-7.
      Tissue: Lung.
    4. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
      Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
      , Gu J., Chen S.-J., Chen Z.
      Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-241.
      Tissue: Umbilical cord blood.
    5. "Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation."
      Liu L.X., Margottin F., Le Gall S., Schwartz O., Selig L., Benarous R., Benichou S.
      J. Biol. Chem. 272:13779-13785(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 218-441, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH HIV-1 NEF, TISSUE SPECIFICITY.
      Tissue: Lymphocyte.

    Entry informationi

    Entry nameiNAP1_HUMAN
    AccessioniPrimary (citable) accession number: Q9BU70
    Secondary accession number(s): Q5T113
    , Q86SK0, Q9NXJ7, Q9P0Q7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3