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Q9BU64 (CENPO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Centromere protein O

Short name=CENP-O
Alternative name(s):
Interphase centromere complex protein 36
Gene names
Name:CENPO
Synonyms:ICEN36, MCM21R
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the kinetochore-bound levels of NDC80 complex. Ref.5 Ref.6 Ref.7 Ref.9

Subunit structure

Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Ref.7 Ref.8

Subcellular location

Nucleus. Chromosomecentromere. Chromosomecentromerekinetochore. Note: The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex. Ref.5 Ref.6 Ref.7 Ref.9

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BU64-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BU64-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MEQANPLRPDGESKG → MAGILASGL
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300Centromere protein O
PRO_0000249502

Regions

Coiled coil18 – 4225 Potential
Coiled coil83 – 10927 Potential

Natural variations

Alternative sequence1 – 1515MEQAN…GESKG → MAGILASGL in isoform 2.
VSP_020446
Natural variant341Q → R. Ref.1
Corresponds to variant rs1550116 [ dbSNP | Ensembl ].
VAR_027420

Experimental info

Sequence conflict2351A → V in BAB55414. Ref.1
Sequence conflict2691A → V in BAB55414. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 05A89E12C6847D81

FASTA30033,786
        10         20         30         40         50         60 
MEQANPLRPD GESKGGVLAH LERLETQVSR SRKQSEELQS VQAQEGALGT KIHKLRRLRD 

        70         80         90        100        110        120 
ELRAVVRHRR ASVKACIANV EPNQTVEINE QEALEEKLEN VKAILQAYHF TGLSGKLTSR 

       130        140        150        160        170        180 
GVCVCISTAF EGNLLDSYFV DLVIQKPLRI HHHSVPVFIP LEEIAAKYLQ TNIQHFLFSL 

       190        200        210        220        230        240 
CEYLNAYSGR KYQADRLQSD FAALLTGPLQ RNPLCNLLSF TYKLDPGGQS FPFCARLLYK 

       250        260        270        280        290        300 
DLTATLPTDV TVTCQGVEVL STSWEEQRAS HETLFCTKPL HQVFASFTRK GEKLDMSLVS 

« Hide

Isoform 2 [UniParc].

Checksum: 76D32EB5C95FD332
Show »

FASTA29432,989

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ARG-34.
Tissue: Placenta.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[5]"The human kinetochore proteins Nnf1R and Mcm21R are required for accurate chromosome segregation."
McAinsh A.D., Meraldi P., Draviam V.M., Toso A., Sorger P.K.
EMBO J. 25:4033-4049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells."
Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K.
Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres."
Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T.
Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH CENPH; CENPI; CENPK; CENPN; CENPP; CENPQ; CENPR AND CENPU, FUNCTION, SUBCELLULAR LOCATION.
[8]"The human CENP-A centromeric nucleosome-associated complex."
Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III, Cleveland D.W.
Nat. Cell Biol. 8:458-469(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE CENPA-CAD COMPLEX WITH CENPI; CENPK; CENPL; CENPP; CENPQ; CENPR AND CENPS.
[9]"The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity."
McClelland S.E., Borusu S., Amaro A.C., Winter J.R., Belwal M., McAinsh A.D., Meraldi P.
EMBO J. 26:5033-5047(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK027859 mRNA. Translation: BAB55414.1.
AK315483 mRNA. Translation: BAG37867.1.
AC012073 Genomic DNA. Translation: AAY14786.1.
AC013459 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00742.1.
CH471053 Genomic DNA. Translation: EAX00743.1.
BC002870 mRNA. Translation: AAH02870.1.
RefSeqNP_001186732.1. NM_001199803.1.
NP_077298.1. NM_024322.2.
UniGeneHs.731569.

3D structure databases

ProteinModelPortalQ9BU64.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122589. 15 interactions.
IntActQ9BU64. 2 interactions.
MINTMINT-1439597.
STRING9606.ENSP00000260662.

PTM databases

PhosphoSiteQ9BU64.

Polymorphism databases

DMDM74733185.

Proteomic databases

PaxDbQ9BU64.
PRIDEQ9BU64.

Protocols and materials databases

DNASU79172.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000260662; ENSP00000260662; ENSG00000138092. [Q9BU64-1]
ENST00000380834; ENSP00000370214; ENSG00000138092. [Q9BU64-1]
ENST00000473706; ENSP00000417787; ENSG00000138092. [Q9BU64-2]
GeneID79172.
KEGGhsa:79172.
UCSCuc002rfp.2. human. [Q9BU64-2]
uc002rfq.2. human. [Q9BU64-1]

Organism-specific databases

CTD79172.
GeneCardsGC02P025016.
HGNCHGNC:28152. CENPO.
HPAHPA052458.
MIM611504. gene.
neXtProtNX_Q9BU64.
PharmGKBPA145149197.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG39904.
HOGENOMHOG000059658.
HOVERGENHBG080687.
InParanoidQ9BU64.
KOK11507.
OMAALSTSWE.
OrthoDBEOG7VX8WJ.
PhylomeDBQ9BU64.
TreeFamTF335524.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.

Gene expression databases

BgeeQ9BU64.
CleanExHS_CENPO.
GenevestigatorQ9BU64.

Family and domain databases

InterProIPR018464. CENP-O.
[Graphical view]
PfamPF09496. CENP-O. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCENPO. human.
GeneWikiCENPO.
GenomeRNAi79172.
NextBio68137.
PROQ9BU64.
SOURCESearch...

Entry information

Entry nameCENPO_HUMAN
AccessionPrimary (citable) accession number: Q9BU64
Secondary accession number(s): B2RDC0 expand/collapse secondary AC list , D6W536, Q53T55, Q96JV3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM