Q9BU64 (CENPO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centromere protein O
Short name=CENP-O

Alternative name(s):
Interphase centromere complex protein 36

Gene names

Name:	CENPO
Synonyms:	ICEN36, MCM21R

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the kinetochore-bound levels of NDC80 complex. Ref.5 Ref.6 Ref.7 Ref.9
Subunit structure	Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Ref.7 Ref.8
Subcellular location	Nucleus. Chromosome › centromere. Chromosome › centromere › kinetochore. Note: The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex. Ref.5 Ref.6 Ref.7 Ref.9

Ontologies

Keywords
Cellular component	Centromere Chromosome Kinetochore Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CENP-A containing nucleosome assembly at centromere Traceable author statement. Source: Reactome cell division Inferred from electronic annotation. Source: InterPro chromosome segregation Inferred from electronic annotation. Source: InterPro mitotic prometaphase Traceable author statement. Source: Reactome
Cellular_component	condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome nucleoplasm Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BU64-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BU64-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MEQANPLRPDGESKG → MAGILASGL

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Centromere protein O		PRO_0000249502
Regions
	Coiled coil	18 – 42	25	Potential
	Coiled coil	83 – 109	27	Potential
Natural variations
	Alternative sequence	1 – 15	15	MEQAN…GESKG → MAGILASGL in isoform 2.		VSP_020446
	Natural variant	34	1	Q → R. Ref.1 Corresponds to variant rs1550116 [ dbSNP | Ensembl ].		VAR_027420
Experimental info
	Sequence conflict	235	1	A → V in BAB55414. Ref.1
	Sequence conflict	269	1	A → V in BAB55414. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 05A89E12C6847D81	FASTA	300	33,786
	10 20 30 40 50 60 MEQANPLRPD GESKGGVLAH LERLETQVSR SRKQSEELQS VQAQEGALGT KIHKLRRLRD 70 80 90 100 110 120 ELRAVVRHRR ASVKACIANV EPNQTVEINE QEALEEKLEN VKAILQAYHF TGLSGKLTSR 130 140 150 160 170 180 GVCVCISTAF EGNLLDSYFV DLVIQKPLRI HHHSVPVFIP LEEIAAKYLQ TNIQHFLFSL 190 200 210 220 230 240 CEYLNAYSGR KYQADRLQSD FAALLTGPLQ RNPLCNLLSF TYKLDPGGQS FPFCARLLYK 250 260 270 280 290 300 DLTATLPTDV TVTCQGVEVL STSWEEQRAS HETLFCTKPL HQVFASFTRK GEKLDMSLVS
	Isoform 2 [UniParc]. Checksum: 76D32EB5C95FD332	FASTA	294	32,989
	10 20 30 40 50 60 MAGILASGLG VLAHLERLET QVSRSRKQSE ELQSVQAQEG ALGTKIHKLR RLRDELRAVV 70 80 90 100 110 120 RHRRASVKAC IANVEPNQTV EINEQEALEE KLENVKAILQ AYHFTGLSGK LTSRGVCVCI 130 140 150 160 170 180 STAFEGNLLD SYFVDLVIQK PLRIHHHSVP VFIPLEEIAA KYLQTNIQHF LFSLCEYLNA 190 200 210 220 230 240 YSGRKYQADR LQSDFAALLT GPLQRNPLCN LLSFTYKLDP GGQSFPFCAR LLYKDLTATL 250 260 270 280 290 PTDVTVTCQG VEVLSTSWEE QRASHETLFC TKPLHQVFAS FTRKGEKLDM SLVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ARG-34. Tissue: Placenta.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[5]	"The human kinetochore proteins Nnf1R and Mcm21R are required for accurate chromosome segregation." McAinsh A.D., Meraldi P., Draviam V.M., Toso A., Sorger P.K. EMBO J. 25:4033-4049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells." Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K. Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres." Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T. Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH CENPH; CENPI; CENPK; CENPN; CENPP; CENPQ; CENPR AND CENPU, FUNCTION, SUBCELLULAR LOCATION.
[8]	"The human CENP-A centromeric nucleosome-associated complex." Foltz D.R., Jansen L.E.T., Black B.E., Bailey A.O., Yates J.R. III, Cleveland D.W. Nat. Cell Biol. 8:458-469(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE CENPA-CAD COMPLEX WITH CENPI; CENPK; CENPL; CENPP; CENPQ; CENPR AND CENPS.
[9]	"The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity." McClelland S.E., Borusu S., Amaro A.C., Winter J.R., Belwal M., McAinsh A.D., Meraldi P. EMBO J. 26:5033-5047(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK027859 mRNA. Translation: BAB55414.1. AK315483 mRNA. Translation: BAG37867.1. AC012073 Genomic DNA. Translation: AAY14786.1. AC013459 Genomic DNA. No translation available. CH471053 Genomic DNA. Translation: EAX00742.1. CH471053 Genomic DNA. Translation: EAX00743.1. BC002870 mRNA. Translation: AAH02870.1.
IPI	IPI00013338. IPI00788136.
RefSeq	NP_001186732.1. NM_001199803.1. NP_077298.1. NM_024322.2.
UniGene	Hs.731569.
3D structure databases
ProteinModelPortal	Q9BU64.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BU64. 2 interactions.
MINT	MINT-1439597.
STRING	9606.ENSP00000260662.
PTM databases
PhosphoSite	Q9BU64.
Polymorphism databases
DMDM	74733185.
Proteomic databases
PaxDb	Q9BU64.
PRIDE	Q9BU64.
Protocols and materials databases
DNASU	79172.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000260662; ENSP00000260662; ENSG00000138092. ENST00000380834; ENSP00000370214; ENSG00000138092. ENST00000473706; ENSP00000417787; ENSG00000138092.
GeneID	79172.
KEGG	hsa:79172.
UCSC	uc002rfp.2. human. uc002rfq.2. human.
Organism-specific databases
CTD	79172.
GeneCards	GC02P025016.
HGNC	HGNC:28152. CENPO.
MIM	611504. gene.
neXtProt	NX_Q9BU64.
PharmGKB	PA145149197.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39904.
HOGENOM	HOG000059658.
HOVERGEN	HBG080687.
InParanoid	Q9BU64.
KO	K11507.
OMA	ALSTSWE.
OrthoDB	EOG4MSD01.
PhylomeDB	Q9BU64.
Enzyme and pathway databases
Reactome	REACT_115566. Cell Cycle. REACT_21300. Mitotic M-M/G1 phases.
Gene expression databases
Bgee	Q9BU64.
CleanEx	HS_CENPO.
Genevestigator	Q9BU64.
GermOnline	ENSG00000138092. Homo sapiens.
Family and domain databases
InterPro	IPR018464. Centromere_CenpO. [Graphical view]
Pfam	PF09496. CENP-O. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CENPO. human.
GenomeRNAi	79172.
NextBio	68137.
SOURCE	Search...

Entry information

Entry name

CENPO_HUMAN

Accession

Primary (citable) accession number: Q9BU64
Secondary accession number(s): B2RDC0 , D6W536, Q53T55, Q96JV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.