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Protein

Centromere protein O

Gene

CENPO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Modulates the kinetochore-bound levels of NDC80 complex.4 Publications

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_355252. RHO GTPases Activate Formins.
REACT_682. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein O
Short name:
CENP-O
Alternative name(s):
Interphase centromere complex protein 36
Gene namesi
Name:CENPO
Synonyms:ICEN36, MCM21R
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:28152. CENPO.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA145149197.

Polymorphism and mutation databases

BioMutaiCENPO.
DMDMi74733185.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Centromere protein OPRO_0000249502Add
BLAST

Proteomic databases

MaxQBiQ9BU64.
PaxDbiQ9BU64.
PRIDEiQ9BU64.

PTM databases

PhosphoSiteiQ9BU64.

Expressioni

Gene expression databases

BgeeiQ9BU64.
CleanExiHS_CENPO.
GenevisibleiQ9BU64. HS.

Interactioni

Subunit structurei

Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CENPPQ6IPU03EBI-745954,EBI-10250303
CEP170P1Q96L145EBI-745954,EBI-743488
FOSQ6FG413EBI-745954,EBI-10198738
NECAB2H3BTW23EBI-745954,EBI-10172876

Protein-protein interaction databases

BioGridi122589. 20 interactions.
IntActiQ9BU64. 5 interactions.
MINTiMINT-1439597.
STRINGi9606.ENSP00000260662.

Structurei

3D structure databases

ProteinModelPortaliQ9BU64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili18 – 4225Sequence AnalysisAdd
BLAST
Coiled coili83 – 10927Sequence AnalysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG39904.
GeneTreeiENSGT00390000016702.
HOGENOMiHOG000059658.
HOVERGENiHBG080687.
InParanoidiQ9BU64.
KOiK11507.
OMAiALSTSWE.
OrthoDBiEOG7VX8WJ.
PhylomeDBiQ9BU64.
TreeFamiTF335524.

Family and domain databases

InterProiIPR018464. CENP-O.
[Graphical view]
PfamiPF09496. CENP-O. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BU64-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEQANPLRPD GESKGGVLAH LERLETQVSR SRKQSEELQS VQAQEGALGT
60 70 80 90 100
KIHKLRRLRD ELRAVVRHRR ASVKACIANV EPNQTVEINE QEALEEKLEN
110 120 130 140 150
VKAILQAYHF TGLSGKLTSR GVCVCISTAF EGNLLDSYFV DLVIQKPLRI
160 170 180 190 200
HHHSVPVFIP LEEIAAKYLQ TNIQHFLFSL CEYLNAYSGR KYQADRLQSD
210 220 230 240 250
FAALLTGPLQ RNPLCNLLSF TYKLDPGGQS FPFCARLLYK DLTATLPTDV
260 270 280 290 300
TVTCQGVEVL STSWEEQRAS HETLFCTKPL HQVFASFTRK GEKLDMSLVS
Length:300
Mass (Da):33,786
Last modified:June 1, 2001 - v1
Checksum:i05A89E12C6847D81
GO
Isoform 2 (identifier: Q9BU64-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MEQANPLRPDGESKG → MAGILASGL

Note: No experimental confirmation available.
Show »
Length:294
Mass (Da):32,989
Checksum:i76D32EB5C95FD332
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti235 – 2351A → V in BAB55414 (PubMed:14702039).Curated
Sequence conflicti269 – 2691A → V in BAB55414 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341Q → R.1 Publication
Corresponds to variant rs1550116 [ dbSNP | Ensembl ].
VAR_027420

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1515MEQAN…GESKG → MAGILASGL in isoform 2. 1 PublicationVSP_020446Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027859 mRNA. Translation: BAB55414.1.
AK315483 mRNA. Translation: BAG37867.1.
AC012073 Genomic DNA. Translation: AAY14786.1.
AC013459 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00742.1.
CH471053 Genomic DNA. Translation: EAX00743.1.
BC002870 mRNA. Translation: AAH02870.1.
CCDSiCCDS1714.1. [Q9BU64-1]
CCDS56113.1. [Q9BU64-2]
RefSeqiNP_001186732.1. NM_001199803.1. [Q9BU64-2]
NP_077298.1. NM_024322.2. [Q9BU64-1]
XP_006712165.1. XM_006712102.2. [Q9BU64-1]
UniGeneiHs.731569.

Genome annotation databases

EnsembliENST00000260662; ENSP00000260662; ENSG00000138092. [Q9BU64-1]
ENST00000380834; ENSP00000370214; ENSG00000138092. [Q9BU64-1]
ENST00000473706; ENSP00000417787; ENSG00000138092. [Q9BU64-2]
GeneIDi79172.
KEGGihsa:79172.
UCSCiuc002rfp.2. human. [Q9BU64-2]
uc002rfq.2. human. [Q9BU64-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027859 mRNA. Translation: BAB55414.1.
AK315483 mRNA. Translation: BAG37867.1.
AC012073 Genomic DNA. Translation: AAY14786.1.
AC013459 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00742.1.
CH471053 Genomic DNA. Translation: EAX00743.1.
BC002870 mRNA. Translation: AAH02870.1.
CCDSiCCDS1714.1. [Q9BU64-1]
CCDS56113.1. [Q9BU64-2]
RefSeqiNP_001186732.1. NM_001199803.1. [Q9BU64-2]
NP_077298.1. NM_024322.2. [Q9BU64-1]
XP_006712165.1. XM_006712102.2. [Q9BU64-1]
UniGeneiHs.731569.

3D structure databases

ProteinModelPortaliQ9BU64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122589. 20 interactions.
IntActiQ9BU64. 5 interactions.
MINTiMINT-1439597.
STRINGi9606.ENSP00000260662.

PTM databases

PhosphoSiteiQ9BU64.

Polymorphism and mutation databases

BioMutaiCENPO.
DMDMi74733185.

Proteomic databases

MaxQBiQ9BU64.
PaxDbiQ9BU64.
PRIDEiQ9BU64.

Protocols and materials databases

DNASUi79172.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260662; ENSP00000260662; ENSG00000138092. [Q9BU64-1]
ENST00000380834; ENSP00000370214; ENSG00000138092. [Q9BU64-1]
ENST00000473706; ENSP00000417787; ENSG00000138092. [Q9BU64-2]
GeneIDi79172.
KEGGihsa:79172.
UCSCiuc002rfp.2. human. [Q9BU64-2]
uc002rfq.2. human. [Q9BU64-1]

Organism-specific databases

CTDi79172.
GeneCardsiGC02P025016.
HGNCiHGNC:28152. CENPO.
MIMi611504. gene.
neXtProtiNX_Q9BU64.
PharmGKBiPA145149197.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG39904.
GeneTreeiENSGT00390000016702.
HOGENOMiHOG000059658.
HOVERGENiHBG080687.
InParanoidiQ9BU64.
KOiK11507.
OMAiALSTSWE.
OrthoDBiEOG7VX8WJ.
PhylomeDBiQ9BU64.
TreeFamiTF335524.

Enzyme and pathway databases

ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.
REACT_355252. RHO GTPases Activate Formins.
REACT_682. Mitotic Prometaphase.

Miscellaneous databases

ChiTaRSiCENPO. human.
GeneWikiiCENPO.
GenomeRNAii79172.
NextBioi68137.
PROiQ9BU64.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BU64.
CleanExiHS_CENPO.
GenevisibleiQ9BU64. HS.

Family and domain databases

InterProiIPR018464. CENP-O.
[Graphical view]
PfamiPF09496. CENP-O. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ARG-34.
    Tissue: Placenta.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  5. "The human kinetochore proteins Nnf1R and Mcm21R are required for accurate chromosome segregation."
    McAinsh A.D., Meraldi P., Draviam V.M., Toso A., Sorger P.K.
    EMBO J. 25:4033-4049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells."
    Izuta H., Ikeno M., Suzuki N., Tomonaga T., Nozaki N., Obuse C., Kisu Y., Goshima N., Nomura F., Nomura N., Yoda K.
    Genes Cells 11:673-684(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres."
    Okada M., Cheeseman I.M., Hori T., Okawa K., McLeod I.X., Yates J.R. III, Desai A., Fukagawa T.
    Nat. Cell Biol. 8:446-457(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH CENPH; CENPI; CENPK; CENPN; CENPP; CENPQ; CENPR AND CENPU, FUNCTION, SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE CENPA-CAD COMPLEX WITH CENPI; CENPK; CENPL; CENPP; CENPQ; CENPR AND CENPS.
  9. "The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity."
    McClelland S.E., Borusu S., Amaro A.C., Winter J.R., Belwal M., McAinsh A.D., Meraldi P.
    EMBO J. 26:5033-5047(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCENPO_HUMAN
AccessioniPrimary (citable) accession number: Q9BU64
Secondary accession number(s): B2RDC0
, D6W536, Q53T55, Q96JV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.