Reviewed, UniProtKB/Swiss-Prot Q9BTY2 (FUCO2_HUMAN)

Names and origin

Protein names

Recommended name:
    Plasma alpha-L-fucosidase
    EC=3.2.1.51
Alternative name(s):
    Alpha-L-fucoside fucohydrolase 2
      Short name=Alpha-L-fucosidase 2

Gene names

Name:	FUCA2
ORF Names:	PSEC0151, UNQ227/PRO260

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
Catalytic activity	An alpha-L-fucoside + H2O = L-fucose + an alcohol.
Subunit structure	Homotetramer By similarity.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 29 family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	fucose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-L-fucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 467	439	Plasma alpha-L-fucosidase		PRO_0000010312
Sites
	Site	294	1	May be important for catalysis By similarity
Amino acid modifications
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
	Glycosylation	239	1	N-linked (GlcNAc...)
	Glycosylation	377	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	233	1	A → E: dbSNP rs11155297.		VAR_055822
	Natural variant	356	1	V → M: dbSNP rs3762002. Ref.3		VAR_022444
	Natural variant	371	1	Y → H: dbSNP rs3762001. Ref.3		VAR_022445
Experimental info
	Sequence conflict	400	1	L → P in BAC11633. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9BTY2-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: FF3B0DA9D818AD85	FASTA	467	54,061
	10 20 30 40 50 60 MRPQELPRLA FPLLLLLLLL LPPPPCPAHS ATRFDPTWES LDARQLPAWF DQAKFGIFIH 70 80 90 100 110 120 WGVFSVPSFG SEWFWWYWQK EKIPKYVEFM KDNYPPSFKY EDFGPLFTAK FFNANQWADI 130 140 150 160 170 180 FQASGAKYIV LTSKHHEGFT LWGSEYSWNW NAIDEGPKRD IVKELEVAIR NRTDLRFGLY 190 200 210 220 230 240 YSLFEWFHPL FLEDESSSFH KRQFPVSKTL PELYELVNNY QPEVLWSDGD GGAPDQYWNS 250 260 270 280 290 300 TGFLAWLYNE SPVRGTVVTN DRWGAGSICK HGGFYTCSDR YNPGHLLPHK WENCMTIDKL 310 320 330 340 350 360 SWGYRREAGI SDYLTIEELV KQLVETVSCG GNLLMNIGPT LDGTISVVFE ERLRQVGSWL 370 380 390 400 410 420 KVNGEAIYET YTWRSQNDTV TPDVWYTSKP KEKLVYAIFL KWPTSGQLFL GHPKAILGAT 430 440 450 460 EVKLLGHGQP LNWISLEQNG IMVELPQLTI HQMPCKWGWA LALTNVI

References

[1]	"HRI human cDNA sequencing project." Ota T., Nishikawa T., Suzuki Y., Kawai-Hio Y., Hayashi K., Ishii S., Saito K., Yamamoto J., Wakamatsu A., Nagai T., Nakamura Y., Nagahari K., Sugano S., Isogai T. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS MET-356 AND HIS-371.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell and Placenta.
[5]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-239, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK075458 mRNA. Translation: BAC11633.1. AY358551 mRNA. Translation: AAQ88915.1. AL031320 Genomic DNA. Translation: CAB53746.1. BC003060 mRNA. Translation: AAH03060.1. BC051268 mRNA. Translation: AAH51268.1.
IPI	IPI00012440.
RefSeq	NP_114409.2.
UniGene	Hs.591332
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH29. Glycoside Hydrolase Family 29.
PTM databases
PhosphoSite	Q9BTY2.
Proteomic databases
PRIDE	Q9BTY2.
Genome annotation databases
Ensembl	ENSG00000001036. Homo sapiens. [Contig view]
GeneID	2519.
KEGG	hsa:2519.
Organism-specific databases
GeneCards	GC06M143857.
H-InvDB	HIX0006268.
HGNC	HGNC:4008. FUCA2.
MIM	136820. gene+phenotype.
PharmGKB	PA28424.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9BTY2.
HOVERGEN	Q9BTY2.
Enzyme and pathway databases
BRENDA	3.2.1.51. 247.
Gene expression databases
ArrayExpress	Q9BTY2.
Bgee	Q9BTY2.
CleanEx	HS_FUCA2.
GermOnline	ENSG00000001036. Homo sapiens.
Family and domain databases
InterPro	IPR000933. Glyco_hydro_29. IPR018526. Glyco_hydro_29_CS. IPR016286. Glyco_hydro_29_sub. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10030. Glyco_hydro_29. 1 hit.
Pfam	PF01120. Alpha_L_fucos. 1 hit. [Graphical view]
PIRSF	PIRSF001092. Alpha-L-fucosidase. 1 hit.
PRINTS	PR00741. GLHYDRLASE29.
SMART	SM00812. Alpha_L_fucos. 1 hit. [Graphical view]
PROSITE	PS00385. ALPHA_L_FUCOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	9919.
SOURCE	Search...

Entry information

Entry name

FUCO2_HUMAN

Accession

Primary (citable) accession number: Q9BTY2
Secondary accession number(s): Q7Z6Y2, Q8NBK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)