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Protein

Mediator of RNA polymerase II transcription subunit 10

Gene

MED10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-212436. Generic Transcription Pathway.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 10
Alternative name(s):
Mediator complex subunit 10
Transformation-related gene 17 protein
Short name:
TRG-17
Transformation-related gene 20 protein
Short name:
TRG-20
Gene namesi
Name:MED10
ORF Names:L6, TRG17, TRG20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:28760. MED10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA144596412.

Polymorphism and mutation databases

BioMutaiMED10.
DMDMi74733145.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 135135Mediator of RNA polymerase II transcription subunit 10PRO_0000303151Add
BLAST

Proteomic databases

EPDiQ9BTT4.
MaxQBiQ9BTT4.
PaxDbiQ9BTT4.
PeptideAtlasiQ9BTT4.
PRIDEiQ9BTT4.
TopDownProteomicsiQ9BTT4.

PTM databases

iPTMnetiQ9BTT4.
PhosphoSiteiQ9BTT4.

Expressioni

Gene expression databases

BgeeiQ9BTT4.
CleanExiHS_MED10.
GenevisibleiQ9BTT4. HS.

Organism-specific databases

HPAiHPA042795.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MED7O435135EBI-394354,EBI-394632

Protein-protein interaction databases

BioGridi123974. 59 interactions.
DIPiDIP-31457N.
IntActiQ9BTT4. 71 interactions.
MINTiMINT-1379304.
STRINGi9606.ENSP00000255764.

Structurei

3D structure databases

ProteinModelPortaliQ9BTT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 10 family.Curated

Phylogenomic databases

eggNOGiKOG3046. Eukaryota.
ENOG4111QA2. LUCA.
GeneTreeiENSGT00390000014501.
HOGENOMiHOG000006789.
HOVERGENiHBG107408.
InParanoidiQ9BTT4.
KOiK15151.
OMAiNQKLNFM.
OrthoDBiEOG79GT8N.
PhylomeDBiQ9BTT4.
TreeFamiTF315096.

Family and domain databases

InterProiIPR019145. Mediator_Med10.
[Graphical view]
PfamiPF09748. Med10. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BTT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLNQK LNFIVTGLQD
60 70 80 90 100
IDKCRQQLHD ITVPLEVFEY IDQGRNPQLY TKECLERALA KNEQVKGKID
110 120 130
TMKKFKSLLI QELSKVFPED MAKYRSIRGE DHPPS
Length:135
Mass (Da):15,688
Last modified:June 1, 2001 - v1
Checksum:iF804CD76770149E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY598325 mRNA. Translation: AAT06736.1.
AY277599 mRNA. Translation: AAQ18038.1.
AY453397 mRNA. Translation: AAS47514.1.
FJ515848 Genomic DNA. Translation: ACS13739.1.
CH471102 Genomic DNA. Translation: EAX08110.1.
BC003353 mRNA. Translation: AAH03353.1.
CCDSiCCDS34134.1.
RefSeqiNP_115662.2. NM_032286.2.
UniGeneiHs.13885.

Genome annotation databases

EnsembliENST00000255764; ENSP00000255764; ENSG00000133398.
GeneIDi84246.
KEGGihsa:84246.
UCSCiuc003jdo.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY598325 mRNA. Translation: AAT06736.1.
AY277599 mRNA. Translation: AAQ18038.1.
AY453397 mRNA. Translation: AAS47514.1.
FJ515848 Genomic DNA. Translation: ACS13739.1.
CH471102 Genomic DNA. Translation: EAX08110.1.
BC003353 mRNA. Translation: AAH03353.1.
CCDSiCCDS34134.1.
RefSeqiNP_115662.2. NM_032286.2.
UniGeneiHs.13885.

3D structure databases

ProteinModelPortaliQ9BTT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123974. 59 interactions.
DIPiDIP-31457N.
IntActiQ9BTT4. 71 interactions.
MINTiMINT-1379304.
STRINGi9606.ENSP00000255764.

PTM databases

iPTMnetiQ9BTT4.
PhosphoSiteiQ9BTT4.

Polymorphism and mutation databases

BioMutaiMED10.
DMDMi74733145.

Proteomic databases

EPDiQ9BTT4.
MaxQBiQ9BTT4.
PaxDbiQ9BTT4.
PeptideAtlasiQ9BTT4.
PRIDEiQ9BTT4.
TopDownProteomicsiQ9BTT4.

Protocols and materials databases

DNASUi84246.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000255764; ENSP00000255764; ENSG00000133398.
GeneIDi84246.
KEGGihsa:84246.
UCSCiuc003jdo.4. human.

Organism-specific databases

CTDi84246.
GeneCardsiMED10.
HGNCiHGNC:28760. MED10.
HPAiHPA042795.
MIMi612382. gene.
neXtProtiNX_Q9BTT4.
PharmGKBiPA144596412.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3046. Eukaryota.
ENOG4111QA2. LUCA.
GeneTreeiENSGT00390000014501.
HOGENOMiHOG000006789.
HOVERGENiHBG107408.
InParanoidiQ9BTT4.
KOiK15151.
OMAiNQKLNFM.
OrthoDBiEOG79GT8N.
PhylomeDBiQ9BTT4.
TreeFamiTF315096.

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-212436. Generic Transcription Pathway.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMED10. human.
GenomeRNAii84246.
PROiQ9BTT4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BTT4.
CleanExiHS_MED10.
GenevisibleiQ9BTT4. HS.

Family and domain databases

InterProiIPR019145. Mediator_Med10.
[Graphical view]
PfamiPF09748. Med10. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Suppression subtractive hybridization and expression profiling identifies a unique set of genes overexpressed in non-small-cell lung cancer."
    Petroziello J., Yamane A., Westendorf L., Thompson M., McDonagh C., Cerveny C., Law C.-L., Wahl A., Carter P.
    Oncogene 23:7734-7745(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of a human transforming gene."
    Kim J.W.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. NHLBI resequencing and genotyping service (RS&G)
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  6. "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation."
    Gu W., Malik S., Ito M., Yuan C.-X., Fondell J.D., Zhang X., Martinez E., Qin J., Roeder R.G.
    Mol. Cell 3:97-108(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SMCC COMPLEX.
  7. "TFIIH is negatively regulated by cdk8-containing mediator complexes."
    Akoulitchev S., Chuikov S., Reinberg D.
    Nature 407:102-106(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED6.
  8. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX.
  9. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCNC; MED1; MED6; MED12; MED13; MED14; MED15; MED16; MED17; MED18; MED19; MED20; MED23; MED24 AND MED26, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMED10_HUMAN
AccessioniPrimary (citable) accession number: Q9BTT4
Secondary accession number(s): C6G491
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.