Q9BTL3 (RAM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNMT-activating mini protein
Short name=RAM

Alternative name(s):
Protein FAM103A1

Gene names

Name:	FAM103A1
Synonyms:	C15orf18

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for efficient mRNA cap methylation. Regulates RNMT expression by a post-transcriptional stabilizing mechanism. Ref.5
Subunit structure	Interacts with RNMT, enhancing its mRNA binding capacity and cap methyltransferase activity. Ref.5
Subcellular location	Nucleus Ref.5.
Sequence similarities	Belongs to the RAM family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	RNMT-activating mini protein		PRO_0000089983
Regions
	Region	1 – 55	55	Interaction with RNMT
	Region	56 – 118	63	RNA-binding
	Compositional bias	91 – 118	28	Gln/Tyr-rich
Amino acid modifications
	Modified residue	36	1	Phosphoserine Ref.2 Ref.3 Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9BTL3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 266B56E66A73A1AE	FASTA	118	14,381
	10 20 30 40 50 60 MTDTAEAVPK FEEMFASRFT ENDKEYQEYL KRPPESPPIV EEWNSRAGGN QRNRGNRLQD 70 80 90 100 110 NRQFRGRDNR WGWPSDNRSN QWHGRSWGNN YPQHRQEPYY PQQYGHYGYN QRPPYGYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Melanoma, Ovarian adenocarcinoma and Skin.
[2]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[3]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[4]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[5]	"RAM/Fam103a1 is required for mRNA cap methylation." Gonatopoulos-Pournatzis T., Dunn S., Bounds R., Cowling V.H. Mol. Cell 44:585-596(2011) [PubMed: 22099306] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING, INTERACTION WITH RNMT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC003627 mRNA. Translation: AAH03627.1. BC027181 mRNA. Translation: AAH27181.1. BC112329 mRNA. Translation: AAI12330.1.
IPI	IPI00027798.
RefSeq	NP_113640.1. NM_031452.2.
UniGene	Hs.727661.
3D structure databases
ProteinModelPortal	Q9BTL3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BTL3. 4 interactions.
MINT	MINT-1452092.
PTM databases
PhosphoSite	Q9BTL3.
Proteomic databases
PRIDE	Q9BTL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000304191; ENSP00000307181; ENSG00000169612.
GeneID	83640.
KEGG	hsa:83640.
UCSC	uc002bjl.1. human.
Organism-specific databases
CTD	83640.
GeneCards	GC15P083654.
H-InvDB	HIX0012517.
HGNC	HGNC:31022. FAM103A1.
HPA	HPA041923. HPA041948.
neXtProt	NX_Q9BTL3.
PharmGKB	PA142671786.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20835.
GeneTree	ENSGT00390000011190.
HOGENOM	HBG126961.
HOVERGEN	HBG081491.
InParanoid	Q9BTL3.
OMA	DNRRGWP.
OrthoDB	EOG44TP98.
PhylomeDB	Q9BTL3.
Gene expression databases
ArrayExpress	Q9BTL3.
Bgee	Q9BTL3.
CleanEx	HS_FAM103A1.
Genevestigator	Q9BTL3.
GermOnline	ENSG00000169612. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
NextBio	72587.

Entry information

Entry name

RAM_HUMAN

Accession

Primary (citable) accession number: Q9BTL3
Secondary accession number(s): Q2M1J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.