ID   NAT9_HUMAN              Reviewed;         207 AA.
AC   Q9BTE0; B2R7F0; Q9BTD0; Q9Y3T3;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   27-MAR-2024, entry version 152.
DE   RecName: Full=Alpha/beta-tubulin-N-acetyltransferase 9 {ECO:0000305|PubMed:33479178};
DE            EC=2.3.1.308 {ECO:0000269|PubMed:33479178};
DE   AltName: Full=Embryo brain-specific protein;
GN   Name=NAT9; Synonyms=EBS;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA   Chen W., Zou X., Zhang J., Ci H.L., Li Y.P.;
RT   "Molecular cloning and primary studies on EBS: a novel human embryo brain-
RT   specific gene.";
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Adrenal gland;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Brain;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Lung, and Muscle;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=33479178; DOI=10.1073/pnas.2010140118;
RA   Mok J.W., Choi K.W.;
RT   "Novel function of N-acetyltransferase for microtubule stability and JNK
RT   signaling in Drosophila organ development.";
RL   Proc. Natl. Acad. Sci. U.S.A. 118:0-0(2021).
CC   -!- FUNCTION: N-acetyltransferase that mediates the acetylation of the N-
CC       terminal residues of alpha- and beta-tubulin.
CC       {ECO:0000269|PubMed:33479178}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + N-terminal L-methionyl-[tubulin] = CoA + H(+) +
CC         N-terminal N(alpha)-acetyl-L-methionyl-[tubulin];
CC         Xref=Rhea:RHEA:69607, Rhea:RHEA-COMP:17729, Rhea:RHEA-COMP:17730,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:64731, ChEBI:CHEBI:133414; EC=2.3.1.308;
CC         Evidence={ECO:0000269|PubMed:33479178};
CC   -!- INTERACTION:
CC       Q9BTE0; O43324: EEF1E1; NbExp=4; IntAct=EBI-711919, EBI-1048486;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9BTE0-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9BTE0-2; Sequence=VSP_025231;
CC   -!- SIMILARITY: Belongs to the acetyltransferase family. GNAT subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AY632082; AAU25819.1; -; mRNA.
DR   EMBL; AK312958; BAG35797.1; -; mRNA.
DR   EMBL; AL050269; CAB43370.1; -; mRNA.
DR   EMBL; CH471099; EAW89197.1; -; Genomic_DNA.
DR   EMBL; BC004195; AAH04195.1; -; mRNA.
DR   EMBL; BC004225; AAH04225.1; -; mRNA.
DR   CCDS; CCDS11706.1; -. [Q9BTE0-1]
DR   CCDS; CCDS77102.1; -. [Q9BTE0-2]
DR   PIR; T08699; T08699.
DR   RefSeq; NP_001292006.1; NM_001305077.1. [Q9BTE0-2]
DR   RefSeq; NP_001292007.1; NM_001305078.1. [Q9BTE0-1]
DR   RefSeq; NP_001292008.1; NM_001305079.1.
DR   RefSeq; NP_001292009.1; NM_001305080.1.
DR   RefSeq; NP_001292010.1; NM_001305081.1.
DR   RefSeq; NP_001292011.1; NM_001305082.1.
DR   RefSeq; NP_001292012.1; NM_001305083.1.
DR   RefSeq; NP_001292013.1; NM_001305084.1.
DR   RefSeq; NP_001292014.1; NM_001305085.1.
DR   RefSeq; NP_001292015.1; NM_001305086.1.
DR   RefSeq; NP_001292016.1; NM_001305087.1.
DR   RefSeq; NP_001292017.1; NM_001305088.1.
DR   RefSeq; NP_056469.2; NM_015654.4. [Q9BTE0-1]
DR   AlphaFoldDB; Q9BTE0; -.
DR   SMR; Q9BTE0; -.
DR   BioGRID; 117582; 20.
DR   IntAct; Q9BTE0; 12.
DR   MINT; Q9BTE0; -.
DR   STRING; 9606.ENSP00000462835; -.
DR   iPTMnet; Q9BTE0; -.
DR   PhosphoSitePlus; Q9BTE0; -.
DR   BioMuta; NAT9; -.
DR   DMDM; 74752333; -.
DR   EPD; Q9BTE0; -.
DR   jPOST; Q9BTE0; -.
DR   MassIVE; Q9BTE0; -.
DR   MaxQB; Q9BTE0; -.
DR   PaxDb; 9606-ENSP00000350467; -.
DR   PeptideAtlas; Q9BTE0; -.
DR   ProteomicsDB; 78979; -. [Q9BTE0-1]
DR   ProteomicsDB; 78980; -. [Q9BTE0-2]
DR   Pumba; Q9BTE0; -.
DR   Antibodypedia; 19466; 83 antibodies from 19 providers.
DR   DNASU; 26151; -.
DR   Ensembl; ENST00000357814.8; ENSP00000350467.3; ENSG00000109065.12. [Q9BTE0-1]
DR   Ensembl; ENST00000580301.5; ENSP00000462019.1; ENSG00000109065.12. [Q9BTE0-2]
DR   GeneID; 26151; -.
DR   KEGG; hsa:26151; -.
DR   MANE-Select; ENST00000357814.8; ENSP00000350467.3; NM_015654.5; NP_056469.2.
DR   UCSC; uc002jlq.3; human. [Q9BTE0-1]
DR   AGR; HGNC:23133; -.
DR   CTD; 26151; -.
DR   DisGeNET; 26151; -.
DR   GeneCards; NAT9; -.
DR   HGNC; HGNC:23133; NAT9.
DR   HPA; ENSG00000109065; Low tissue specificity.
DR   neXtProt; NX_Q9BTE0; -.
DR   OpenTargets; ENSG00000109065; -.
DR   PharmGKB; PA142671281; -.
DR   VEuPathDB; HostDB:ENSG00000109065; -.
DR   eggNOG; KOG4135; Eukaryota.
DR   GeneTree; ENSGT00390000012745; -.
DR   InParanoid; Q9BTE0; -.
DR   OrthoDB; 7623at2759; -.
DR   PhylomeDB; Q9BTE0; -.
DR   TreeFam; TF315021; -.
DR   BioCyc; MetaCyc:ENSG00000109065-MONOMER; -.
DR   PathwayCommons; Q9BTE0; -.
DR   SignaLink; Q9BTE0; -.
DR   BioGRID-ORCS; 26151; 8 hits in 1067 CRISPR screens.
DR   ChiTaRS; NAT9; human.
DR   GeneWiki; NAT9; -.
DR   GenomeRNAi; 26151; -.
DR   Pharos; Q9BTE0; Tbio.
DR   PRO; PR:Q9BTE0; -.
DR   Proteomes; UP000005640; Chromosome 17.
DR   RNAct; Q9BTE0; Protein.
DR   Bgee; ENSG00000109065; Expressed in adenohypophysis and 197 other cell types or tissues.
DR   ExpressionAtlas; Q9BTE0; baseline and differential.
DR   GO; GO:0032991; C:protein-containing complex; IDA:LIFEdb.
DR   GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR   Gene3D; 3.40.630.30; -; 1.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000182; GNAT_dom.
DR   InterPro; IPR039135; NAT9-like.
DR   PANTHER; PTHR13256:SF16; ALPHA_BETA-TUBULIN-N-ACETYLTRANSFERASE 9; 1.
DR   PANTHER; PTHR13256; N-ACETYLTRANSFERASE 9; 1.
DR   Pfam; PF13302; Acetyltransf_3; 1.
DR   SUPFAM; SSF55729; Acyl-CoA N-acyltransferases (Nat); 1.
DR   Genevisible; Q9BTE0; HS.
PE   1: Evidence at protein level;
KW   Acyltransferase; Alternative splicing; Reference proteome; Transferase.
FT   CHAIN           1..207
FT                   /note="Alpha/beta-tubulin-N-acetyltransferase 9"
FT                   /id="PRO_0000286874"
FT   DOMAIN          35..180
FT                   /note="N-acetyltransferase"
FT   VAR_SEQ         26
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039,
FT                   ECO:0000303|PubMed:15489334, ECO:0000303|PubMed:17974005,
FT                   ECO:0000303|Ref.1"
FT                   /id="VSP_025231"
FT   VARIANT         56
FT                   /note="C -> R (in dbSNP:rs2305213)"
FT                   /id="VAR_032225"
FT   CONFLICT        126
FT                   /note="L -> P (in Ref. 3; CAB43370)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   207 AA;  23361 MW;  1265AC8D2B2B376F CRC64;
     MRLNQNTLLL GKKVVLVPYT SEHVPSRYHE WMKSEELQRL TASEPLTLEQ EYAMQCSWQE
     DADKCTFIVL DAEKWQAQPG ATEESCMVGD VNLFLTDLED LTLGEIEVMI AEPSCRGKGL
     GTEAVLAMLS YGVTTLGLTK FEAKIGQGNE PSIRMFQKLH FEQVATSSVF QEVTLRLTVS
     ESEHQWLLEQ TSHVEEKPYR DGSAEPC
//