Q9BT92 (TCHP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trichoplein keratin filament-binding protein
Short name=Protein TCHP

Alternative name(s):
Mitochondrial protein with oncostatic activity
Short name=Mitostatin
Tumor suppressor protein

Gene names

Name:

TCHP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells. Ref.1 Ref.5
Subunit structure	Interacts specifically with keratin proteins including, KRT5, KRT6A, KRT8, KRT14, KRT16 and KRT18. Ref.1
Subcellular location	Cytoplasm › cytoskeleton. Cytoplasm. Cell membrane. Mitochondrion Ref.1 Ref.5.
Tissue specificity	Expressed at high levels in normal urothelial and breast epithelial cells. Also expressed in the smooth muscle and endothelial cells. Reduced expression seen in advanced bladder and breast carcinomas (at protein level). Ubiquitous. Expressed at highest levels in the heart, skeletal muscle, kidney, liver and testis. Ref.5
Sequence similarities	Belongs to the TCHP family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane Mitochondrion
Coding sequence diversity	Polymorphism
Disease	Tumor suppressor
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from direct assay Ref.5. Source: UniProtKB negative regulation of cell growth Inferred from direct assay Ref.5. Source: UniProtKB
Cellular_component	apical cortex Inferred from direct assay Ref.1. Source: HGNC centrosome Inferred from direct assay PubMed 21399614. Source: UniProtKB keratin filament Inferred from direct assay Ref.1. Source: HGNC mitochondrion Inferred from direct assay Ref.5. Source: UniProtKB plasma membrane Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Trichoplein keratin filament-binding protein		PRO_0000292609
Regions
	Region	73 – 498	426	Interaction with keratin proteins Ref.1
	Region	259 – 425	167	Trichohyalin/plectin homology domain Ref.1
	Coiled coil	11 – 39	29	Potential
	Coiled coil	66 – 136	71	Potential
	Coiled coil	163 – 353	191	Potential
	Coiled coil	380 – 479	100	Potential
	Compositional bias	70 – 482	413	Glu-rich
Natural variations
	Natural variant	44	1	S → P in a gastric carcinoma sample. Ref.5		VAR_064056
	Natural variant	93	1	E → K in a pancreatic carcinoma sample. Ref.5		VAR_064057
	Natural variant	127	1	K → R. Corresponds to variant rs10774978 [ dbSNP | Ensembl ].		VAR_053924
	Natural variant	417	1	E → K. Corresponds to variant rs16940680 [ dbSNP | Ensembl ].		VAR_053925
Experimental info
	Sequence conflict	326	1	A → V in BAC03960. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9BT92 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7D1E8A66C56516F8	FASTA	498	61,072
	10 20 30 40 50 60 MALPTLPSYW CSQQRLNQQL ARQREQEARL RQQWEQNSRY FRMSDICSSK QAEWSSKTSY 70 80 90 100 110 120 QRSMHAYQRE KMKEEKRRSL EARREKLRQL MQEEQDLLAR ELEELRLSMN LQERRIREQH 130 140 150 160 170 180 GKLKSAKEEQ RKLIAEQLLY EHWKKNNPKL REMELDLHQK HVVNSWEMQK EEKKQQEATA 190 200 210 220 230 240 EQENKRYENE YERARREALE RMKAEEERRQ LEDKLQAEAL LQQMEELKLK EVEATKLKKE 250 260 270 280 290 300 QENLLKQRWE LERLEEERKQ MEAFRQKAEL GRFLRHQYNA QLSRRTQQIQ EELEADRRIL 310 320 330 340 350 360 QALLEKEDES QRLHLARREQ VMADVAWMKQ AIEEQLQLER AREAELQMLL REEAKEMWEK 370 380 390 400 410 420 REAEWARERS ARDRLMSEVL TGRQQQIQEK IEQNRRAQEE SLKHREQLIR NLEEVRELAR 430 440 450 460 470 480 REKEESEKLK SARKQELEAQ VAERRLQAWE ADQQEEEEEE EARRVEQLSD ALLQQEAETM 490 AEQGYRPKPY GHPKIAWN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of trichoplein, a novel keratin filament-binding protein." Nishizawa M., Izawa I., Inoko A., Hayashi Y., Nagata K., Yokoyama T., Usukura J., Inagaki M. J. Cell Sci. 118:1081-1090(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH KRT5; KRT6A; KRT8; KRT14; KRT16 AND KRT18, SUBCELLULAR LOCATION. Tissue: Liver.
[2]	"A novel tumor suppressor gene at 12q (TS12Q)." Baffa R., Croce C.M., Gomella L.G., Iozzo R.V., Vecchione A. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Small intestine.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[5]	"MITOSTATIN, a putative tumor suppressor on chromosome 12q24.1, is downregulated in human bladder and breast cancer." Vecchione A., Fassan M., Anesti V., Morrione A., Goldoni S., Baldassarre G., Byrne D., D'Arca D., Palazzo J.P., Lloyd J., Scorrano L., Gomella L.G., Iozzo R.V., Baffa R. Oncogene 28:257-269(2009) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS PRO-44 AND LYS-93, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY007230 mRNA. Translation: AAG12971.1. AK092736 mRNA. Translation: BAC03960.1. BC004285 mRNA. Translation: AAH04285.1.
IPI	IPI00031104.
RefSeq	NP_001137324.1. NM_001143852.1. NP_115676.1. NM_032300.4.
UniGene	Hs.410924.
3D structure databases
HSSP	HSSP built from PDB template 1GMJ based on UniProtKB P01096.
ProteinModelPortal	Q9BT92.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BT92. 6 interactions.
MINT	MINT-1452298.
STRING	9606.ENSP00000324404.
PTM databases
PhosphoSite	Q9BT92.
Polymorphism databases
DMDM	74733103.
Proteomic databases
PaxDb	Q9BT92.
PRIDE	Q9BT92.
Protocols and materials databases
DNASU	84260.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000312777; ENSP00000324404; ENSG00000139437. ENST00000405876; ENSP00000384520; ENSG00000139437. ENST00000544838; ENSP00000440838; ENSG00000139437.
GeneID	84260.
KEGG	hsa:84260.
UCSC	uc001tpn.3. human.
Organism-specific databases
CTD	84260.
GeneCards	GC12P110338.
HGNC	HGNC:28135. TCHP.
HPA	HPA038638.
MIM	612654. gene.
neXtProt	NX_Q9BT92.
PharmGKB	PA143485629.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG129785.
HOGENOM	HOG000006708.
HOVERGEN	HBG097262.
InParanoid	Q9BT92.
KO	K16811.
OMA	TLPSYWC.
OrthoDB	EOG44BB2W.
PhylomeDB	Q9BT92.
Gene expression databases
ArrayExpress	Q9BT92.
Bgee	Q9BT92.
CleanEx	HS_TCHP.
Genevestigator	Q9BT92.
Family and domain databases
InterPro	IPR026773. TCHP/CCDC11. [Graphical view]
PANTHER	PTHR31183. PTHR31183. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	TCHP. human.
GenomeRNAi	84260.
NextBio	73786.
SOURCE	Search...

Entry information

Entry name

TCHP_HUMAN

Accession

Primary (citable) accession number: Q9BT92
Secondary accession number(s): Q8NAG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.