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Protein

Trichoplein keratin filament-binding protein

Gene

TCHP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells.2 Publications

GO - Biological processi

  1. apoptotic process Source: UniProtKB
  2. negative regulation of cell growth Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Trichoplein keratin filament-binding protein
Short name:
Protein TCHP
Alternative name(s):
Mitochondrial protein with oncostatic activity
Short name:
Mitostatin
Tumor suppressor protein
Gene namesi
Name:TCHPImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:28135. TCHP.

Subcellular locationi

GO - Cellular componenti

  1. apical cortex Source: HGNC
  2. centrosome Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. keratin filament Source: HGNC
  5. mitochondrion Source: UniProtKB
  6. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

PharmGKBiPA143485629.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Trichoplein keratin filament-binding proteinPRO_0000292609Add
BLAST

Proteomic databases

MaxQBiQ9BT92.
PaxDbiQ9BT92.
PRIDEiQ9BT92.

PTM databases

PhosphoSiteiQ9BT92.

Expressioni

Tissue specificityi

Expressed at high levels in normal urothelial and breast epithelial cells. Also expressed in the smooth muscle and endothelial cells. Reduced expression seen in advanced bladder and breast carcinomas (at protein level). Ubiquitous. Expressed at highest levels in the heart, skeletal muscle, kidney, liver and testis.1 Publication

Gene expression databases

BgeeiQ9BT92.
CleanExiHS_TCHP.
ExpressionAtlasiQ9BT92. baseline and differential.
GenevestigatoriQ9BT92.

Organism-specific databases

HPAiHPA038638.

Interactioni

Subunit structurei

Interacts specifically with keratin proteins including, KRT5, KRT6A, KRT8, KRT14, KRT16 and KRT18.1 Publication

Protein-protein interaction databases

BioGridi123987. 20 interactions.
IntActiQ9BT92. 8 interactions.
MINTiMINT-1452298.
STRINGi9606.ENSP00000324404.

Structurei

3D structure databases

ProteinModelPortaliQ9BT92.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni73 – 498426Interaction with keratin proteins1 PublicationAdd
BLAST
Regioni259 – 425167Trichohyalin/plectin homology domain1 PublicationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili11 – 3929Sequence AnalysisAdd
BLAST
Coiled coili66 – 13671Sequence AnalysisAdd
BLAST
Coiled coili163 – 353191Sequence AnalysisAdd
BLAST
Coiled coili380 – 479100Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi70 – 482413Glu-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the TCHP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG129785.
GeneTreeiENSGT00730000111170.
HOGENOMiHOG000006708.
HOVERGENiHBG097262.
InParanoidiQ9BT92.
KOiK16811.
OMAiPKLREME.
OrthoDBiEOG7Z0JX0.
PhylomeDBiQ9BT92.
TreeFamiTF329032.

Family and domain databases

InterProiIPR026773. TCHP.
[Graphical view]
PANTHERiPTHR31183:SF2. PTHR31183:SF2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BT92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPTLPSYW CSQQRLNQQL ARQREQEARL RQQWEQNSRY FRMSDICSSK
60 70 80 90 100
QAEWSSKTSY QRSMHAYQRE KMKEEKRRSL EARREKLRQL MQEEQDLLAR
110 120 130 140 150
ELEELRLSMN LQERRIREQH GKLKSAKEEQ RKLIAEQLLY EHWKKNNPKL
160 170 180 190 200
REMELDLHQK HVVNSWEMQK EEKKQQEATA EQENKRYENE YERARREALE
210 220 230 240 250
RMKAEEERRQ LEDKLQAEAL LQQMEELKLK EVEATKLKKE QENLLKQRWE
260 270 280 290 300
LERLEEERKQ MEAFRQKAEL GRFLRHQYNA QLSRRTQQIQ EELEADRRIL
310 320 330 340 350
QALLEKEDES QRLHLARREQ VMADVAWMKQ AIEEQLQLER AREAELQMLL
360 370 380 390 400
REEAKEMWEK REAEWARERS ARDRLMSEVL TGRQQQIQEK IEQNRRAQEE
410 420 430 440 450
SLKHREQLIR NLEEVRELAR REKEESEKLK SARKQELEAQ VAERRLQAWE
460 470 480 490
ADQQEEEEEE EARRVEQLSD ALLQQEAETM AEQGYRPKPY GHPKIAWN
Length:498
Mass (Da):61,072
Last modified:June 1, 2001 - v1
Checksum:i7D1E8A66C56516F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti326 – 3261A → V in BAC03960 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti44 – 441S → P in a gastric carcinoma sample. 1 Publication
VAR_064056
Natural varianti93 – 931E → K in a pancreatic carcinoma sample. 1 Publication
VAR_064057
Natural varianti127 – 1271K → R.
Corresponds to variant rs10774978 [ dbSNP | Ensembl ].
VAR_053924
Natural varianti417 – 4171E → K.
Corresponds to variant rs16940680 [ dbSNP | Ensembl ].
VAR_053925

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY007230 mRNA. Translation: AAG12971.1.
AK092736 mRNA. Translation: BAC03960.1.
BC004285 mRNA. Translation: AAH04285.1.
CCDSiCCDS9137.1.
RefSeqiNP_001137324.1. NM_001143852.1.
NP_115676.1. NM_032300.4.
UniGeneiHs.410924.

Genome annotation databases

EnsembliENST00000312777; ENSP00000324404; ENSG00000139437.
ENST00000405876; ENSP00000384520; ENSG00000139437.
ENST00000544838; ENSP00000440838; ENSG00000139437.
GeneIDi84260.
KEGGihsa:84260.
UCSCiuc001tpn.3. human.

Polymorphism databases

DMDMi74733103.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY007230 mRNA. Translation: AAG12971.1.
AK092736 mRNA. Translation: BAC03960.1.
BC004285 mRNA. Translation: AAH04285.1.
CCDSiCCDS9137.1.
RefSeqiNP_001137324.1. NM_001143852.1.
NP_115676.1. NM_032300.4.
UniGeneiHs.410924.

3D structure databases

ProteinModelPortaliQ9BT92.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123987. 20 interactions.
IntActiQ9BT92. 8 interactions.
MINTiMINT-1452298.
STRINGi9606.ENSP00000324404.

PTM databases

PhosphoSiteiQ9BT92.

Polymorphism databases

DMDMi74733103.

Proteomic databases

MaxQBiQ9BT92.
PaxDbiQ9BT92.
PRIDEiQ9BT92.

Protocols and materials databases

DNASUi84260.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312777; ENSP00000324404; ENSG00000139437.
ENST00000405876; ENSP00000384520; ENSG00000139437.
ENST00000544838; ENSP00000440838; ENSG00000139437.
GeneIDi84260.
KEGGihsa:84260.
UCSCiuc001tpn.3. human.

Organism-specific databases

CTDi84260.
GeneCardsiGC12P110338.
HGNCiHGNC:28135. TCHP.
HPAiHPA038638.
MIMi612654. gene.
neXtProtiNX_Q9BT92.
PharmGKBiPA143485629.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG129785.
GeneTreeiENSGT00730000111170.
HOGENOMiHOG000006708.
HOVERGENiHBG097262.
InParanoidiQ9BT92.
KOiK16811.
OMAiPKLREME.
OrthoDBiEOG7Z0JX0.
PhylomeDBiQ9BT92.
TreeFamiTF329032.

Miscellaneous databases

ChiTaRSiTCHP. human.
GeneWikiiTCHP.
GenomeRNAii84260.
NextBioi73786.
PROiQ9BT92.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BT92.
CleanExiHS_TCHP.
ExpressionAtlasiQ9BT92. baseline and differential.
GenevestigatoriQ9BT92.

Family and domain databases

InterProiIPR026773. TCHP.
[Graphical view]
PANTHERiPTHR31183:SF2. PTHR31183:SF2. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of trichoplein, a novel keratin filament-binding protein."
    Nishizawa M., Izawa I., Inoko A., Hayashi Y., Nagata K., Yokoyama T., Usukura J., Inagaki M.
    J. Cell Sci. 118:1081-1090(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH KRT5; KRT6A; KRT8; KRT14; KRT16 AND KRT18, SUBCELLULAR LOCATION.
    Tissue: Liver1 Publication.
  2. "A novel tumor suppressor gene at 12q (TS12Q)."
    Baffa R., Croce C.M., Gomella L.G., Iozzo R.V., Vecchione A.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Small intestineImported.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: SkinImported.
  5. "MITOSTATIN, a putative tumor suppressor on chromosome 12q24.1, is downregulated in human bladder and breast cancer."
    Vecchione A., Fassan M., Anesti V., Morrione A., Goldoni S., Baldassarre G., Byrne D., D'Arca D., Palazzo J.P., Lloyd J., Scorrano L., Gomella L.G., Iozzo R.V., Baffa R.
    Oncogene 28:257-269(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS PRO-44 AND LYS-93, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTCHP_HUMAN
AccessioniPrimary (citable) accession number: Q9BT92
Secondary accession number(s): Q8NAG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.