Reviewed,
UniProtKB/Swiss-Prot Q9BT40 (INP5K_HUMAN)
Last modified
February 9, 2010.
Version 71.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Inositol polyphosphate 5-phosphatase K EC=3.1.3.56 Alternative name(s): Skeletal muscle and kidney-enriched inositol phosphatase | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 448 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Inositol 5-phosphatase which acts on inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-triphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol 1,4,5-trisphosphate. May negatively regulate assembly of the actin cytoskeleton. Ref.1 |
| Catalytic activity | D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate. Ref.1 |
| Subcellular location | Endoplasmic reticulum. Note: Following stimulation with EGF, translocates to membrane ruffles. Ref.6 |
| Tissue specificity | Ubiquitously expressed with highest levels in skeletal muscle, heart and kidney. Ref.1 |
| Sequence similarities | Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | actin cytoskeleton organization Ref.1 Non-traceable author statement. Source: UniProtKB |
| Cellular component | cytosol Ref.1 Inferred from direct assay. Source: UniProtKB endoplasmic reticulum Ref.6Inferred from direct assay. Source: MGI membrane fraction Ref.6Inferred from direct assay. Source: MGI neuron projection Ref.1Inferred from direct assay. Source: UniProtKB ruffle Ref.6Inferred from direct assay. Source: MGI |
| Molecular function | inositol bisphosphate phosphatase activity Ref.1 Inferred from direct assay. Source: UniProtKB inositol-polyphosphate 5-phosphatase activityInferred from electronic annotation. Source: EC lipid phosphatase activity Ref.1Non-traceable author statement. Source: UniProtKB protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| EPPK1 | P58107 | 1 | EBI-749162,EBI-297954 | |
| LNX1 | Q8TBB1 | 1 | EBI-749162,EBI-739832 | |
| PDXDC1 | Q6P996 | 1 | EBI-749162,EBI-1049813 | |
| PYGM | P11217 | 1 | EBI-749162,EBI-357469 |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 Ref.1 (identifier: Q9BT40-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 Ref.1 (identifier: Q9BT40-2) The sequence of this isoform differs from the canonical sequence as follows: 1-76: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 448 | 448 | Inositol polyphosphate 5-phosphatase K | PRO_0000209727 | |||||
Regions | |||||||||
| Region | 16 – 318 | 303 | Catalytic Potential | ||||||
| Region | 321 – 448 | 128 | Required for ruffle localization Ref.6 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 76 | 76 | Missing in isoform 2. Ref.1 | VSP_050612 | |||||
| Natural variant | 315 | 1 | S → F in a breast cancer sample; somatic mutation. Ref.7 | VAR_036497 | |||||
Experimental info | |||||||||
| Mutagenesis | 349 | 1 | Y → A or F: No effect on EGF-induced ruffle localization. Ref.6 | ||||||
| Mutagenesis | 361 | 1 | D → A: Significant decrease in EGF-induced ruffle localization. Ref.6 | ||||||
| Mutagenesis | 362 | 1 | W → A: Significant decrease in EGF-induced ruffle localization. Ref.6 | ||||||
| Mutagenesis | 376 | 1 | Y → A or F: No effect on EGF-induced ruffle localization. Ref.6 | ||||||
| Sequence conflict | 120 | 1 | T → A in AAB03214. Ref.5 | ||||||
| Sequence conflict | 416 | 1 | S → R in BAA92340. Ref.1 | ||||||
| Sequence conflict | 416 | 1 | S → R in BAA92341. Ref.1 | ||||||
| Sequence conflict | 416 | 1 | S → R in BAA92342. Ref.1 | ||||||
| Sequence conflict | 416 | 1 | S → R in AAB03214. Ref.5 | ||||||
| Sequence conflict | 426 | 1 | S → R in BAA92340. Ref.1 | ||||||
| Sequence conflict | 426 | 1 | S → R in BAA92341. Ref.1 | ||||||
| Sequence conflict | 426 | 1 | S → R in BAA92342. Ref.1 | ||||||
| Sequence conflict | 426 | 1 | S → R in AAB03214. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification and characterization of a novel inositol polyphosphate 5-phosphatase." Ijuin T., Mochizuki Y., Fukami K., Funaki M., Asano T., Takenawa T. J. Biol. Chem. 275:10870-10875(2000) [PubMed: 10753883] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Testis. |
| [2] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Thymus. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye. |
| [5] | Nussbaum R.L. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-448. Tissue: Brain. |
| [6] | "Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase SKIP localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal growth factor stimulation." Gurung R., Tan A., Ooms L.M., McGrath M.J., Huysmans R.D., Munday A.D., Prescott M., Whisstock J.C., Mitchell C.A. J. Biol. Chem. 278:11376-11385(2003) [PubMed: 12536145] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-349; ASP-361; TRP-362 AND TYR-376. |
| [7] | "The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E.Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] PHE-315. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB036829 mRNA. Translation: BAA92340.1. AB036830 mRNA. Translation: BAA92341.1. AB036831 Genomic DNA. Translation: BAA92342.1. AK312585 mRNA. Translation: BAG35479.1. AK312844 mRNA. Translation: BAG35697.1. CH471108 Genomic DNA. Translation: EAW90614.1. CH471108 Genomic DNA. Translation: EAW90615.1. BC004362 mRNA. Translation: AAH04362.1. U45973 mRNA. Translation: AAB03214.1. |
| IPI | IPI00374574. IPI00374575. |
| RefSeq | NP_001129114.1. NP_057616.2. NP_570122.1. |
| UniGene | Hs.632238 |
3D structure databases | |
| SMR | Q9BT40. Positions 3-326. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9BT40. 6 interactions. |
| STRING | Q9BT40. |
Proteomic databases | |
| PRIDE | Q9BT40. |
Genome annotation databases | |
| Ensembl | ENST00000350761; ENSP00000254712; ENSG00000132376; Homo sapiens. [Genome view] |
| GeneID | 51763. |
| KEGG | hsa:51763. |
| UCSC | uc002fsq.1. human. uc002fsr.1. human. |
Organism-specific databases | |
| CTD | 51763. |
| GeneCards | GC17M001345. |
| HGNC | HGNC:33882. INPP5K. |
| MIM | 607875. gene. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG04880. |
| HOGENOM | HBG443574. |
| HOVERGEN | Q9BT40. |
| InParanoid | Q9BT40. |
| OMA | DRILEMQ. |
| OrthoDB | EOG9WWV49. |
| PhylomeDB | Q9BT40. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:ENSG00000132376-MONOMER. |
| BRENDA | 3.1.3.56. 247. |
Gene expression databases | |
| ArrayExpress | Q9BT40. |
| Bgee | Q9BT40. |
| CleanEx | HS_INPP5K. |
| Genevestigator | Q9BT40. |
| GermOnline | ENSG00000132376. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. [Graphical view] |
| Pfam | PF03372. Exo_endo_phos. 1 hit. [Graphical view] |
| SMART | SM00128. IPPc. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 55878. |
| SOURCE | Search... |
Entry information
| Entry name | INP5K_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9BT40 Secondary accession number(s): B2R6I2 Q9P2R5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 17 Human chromosome 17: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


