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Q9BT40

- INP5K_HUMAN

UniProt

Q9BT40 - INP5K_HUMAN

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Protein

Inositol polyphosphate 5-phosphatase K

Gene

INPP5K

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inositol 5-phosphatase which acts on inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol 1,4,5-trisphosphate. May negatively regulate assembly of the actin cytoskeleton.1 Publication

Catalytic activityi

D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate.1 Publication

GO - Molecular functioni

  1. inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity Source: UniProtKB-EC
  2. inositol-1,4,5-trisphosphate 5-phosphatase activity Source: UniProtKB-EC
  3. inositol bisphosphate phosphatase activity Source: MGI
  4. inositol-polyphosphate 5-phosphatase activity Source: UniProtKB-EC
  5. inositol trisphosphate phosphatase activity Source: MGI
  6. lipid phosphatase activity Source: UniProtKB
  7. phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity Source: UniProtKB
  8. phosphatidylinositol phosphate 5-phosphatase activity Source: UniProtKB
  9. phosphatidylinositol trisphosphate phosphatase activity Source: UniProtKB
  10. vasopressin receptor activity Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton organization Source: UniProtKB
  2. cellular response to cAMP Source: UniProtKB
  3. cellular response to epidermal growth factor stimulus Source: UniProtKB
  4. cellular response to hormone stimulus Source: UniProtKB
  5. cellular response to insulin stimulus Source: UniProtKB
  6. cellular response to tumor necrosis factor Source: UniProtKB
  7. dephosphorylation Source: GOC
  8. glucose homeostasis Source: UniProtKB
  9. G-protein coupled receptor signaling pathway Source: UniProtKB
  10. inositol phosphate dephosphorylation Source: MGI
  11. in utero embryonic development Source: Ensembl
  12. negative regulation by host of viral transcription Source: UniProtKB
  13. negative regulation of calcium ion transport Source: UniProtKB
  14. negative regulation of dephosphorylation Source: UniProtKB
  15. negative regulation of glucose transport Source: UniProtKB
  16. negative regulation of glycogen (starch) synthase activity Source: UniProtKB
  17. negative regulation of glycogen biosynthetic process Source: UniProtKB
  18. negative regulation of insulin receptor signaling pathway Source: UniProtKB
  19. negative regulation of MAP kinase activity Source: UniProtKB
  20. negative regulation of peptidyl-serine phosphorylation Source: UniProtKB
  21. negative regulation of peptidyl-threonine phosphorylation Source: UniProtKB
  22. negative regulation of protein kinase activity Source: UniProtKB
  23. negative regulation of protein kinase B signaling Source: UniProtKB
  24. negative regulation of protein phosphorylation Source: UniProtKB
  25. negative regulation of protein targeting to membrane Source: UniProtKB
  26. negative regulation of single stranded viral RNA replication via double stranded DNA intermediate Source: UniProtKB
  27. negative regulation of stress fiber assembly Source: UniProtKB
  28. negative regulation of transcription, DNA-templated Source: UniProtKB
  29. phosphatidylinositol biosynthetic process Source: Reactome
  30. phosphatidylinositol dephosphorylation Source: UniProtKB
  31. phospholipid metabolic process Source: Reactome
  32. positive regulation of renal water transport Source: UniProtKB
  33. positive regulation of transcription, DNA-templated Source: UniProtKB
  34. positive regulation of urine volume Source: UniProtKB
  35. protein targeting to plasma membrane Source: UniProtKB
  36. regulation of glycogen biosynthetic process Source: UniProtKB
  37. ruffle assembly Source: UniProtKB
  38. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:HS05626-MONOMER.
ReactomeiREACT_121025. Synthesis of PIPs at the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol polyphosphate 5-phosphatase K (EC:3.1.3.56)
Alternative name(s):
Skeletal muscle and kidney-enriched inositol phosphatase
Gene namesi
Name:INPP5K
Synonyms:PPS, SKIP
OrganismiHomo sapiens (Human)Imported
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:33882. INPP5K.

Subcellular locationi

Endoplasmic reticulum 1 Publication
Note: Following stimulation with EGF, translocates to membrane ruffles.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: UniProtKB
  3. endoplasmic reticulum Source: UniProtKB
  4. membrane Source: UniProtKB
  5. neuron projection Source: UniProtKB
  6. nucleus Source: UniProtKB
  7. perinuclear region of cytoplasm Source: UniProtKB
  8. plasma membrane Source: UniProtKB
  9. ruffle Source: MGI
  10. ruffle membrane Source: UniProtKB
  11. trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi349 – 3491Y → A or F: No effect on EGF-induced ruffle localization. 1 Publication
Mutagenesisi361 – 3611D → A: Significant decrease in EGF-induced ruffle localization. 1 Publication
Mutagenesisi362 – 3621W → A: Significant decrease in EGF-induced ruffle localization. 1 Publication
Mutagenesisi376 – 3761Y → A or F: No effect on EGF-induced ruffle localization. 1 Publication

Organism-specific databases

PharmGKBiPA164720951.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448Inositol polyphosphate 5-phosphatase KPRO_0000209727Add
BLAST

Proteomic databases

MaxQBiQ9BT40.
PaxDbiQ9BT40.
PRIDEiQ9BT40.

PTM databases

PhosphoSiteiQ9BT40.

Expressioni

Tissue specificityi

Ubiquitously expressed with highest levels in skeletal muscle, heart and kidney.1 Publication

Gene expression databases

BgeeiQ9BT40.
CleanExiHS_INPP5K.
ExpressionAtlasiQ9BT40. baseline and differential.
GenevestigatoriQ9BT40.

Organism-specific databases

HPAiHPA031044.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LNX1Q8TBB13EBI-749162,EBI-739832

Protein-protein interaction databases

BioGridi119720. 20 interactions.
IntActiQ9BT40. 13 interactions.
MINTiMINT-1445023.
STRINGi9606.ENSP00000254712.

Structurei

3D structure databases

ProteinModelPortaliQ9BT40.
SMRiQ9BT40. Positions 20-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 318303CatalyticSequence AnalysisAdd
BLAST
Regioni321 – 448128Required for ruffle localizationAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5411.
GeneTreeiENSGT00760000119075.
HOGENOMiHOG000046051.
HOVERGENiHBG082135.
InParanoidiQ9BT40.
KOiK01106.
OMAiGLYGYWG.
PhylomeDBiQ9BT40.
TreeFamiTF317034.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SMARTiSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q9BT40) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRKLSGPK GRRLSIHVVT WNVASAAPPL DLSDLLQLNN RNLNLDIYVI
60 70 80 90 100
GLQELNSGII SLLSDAAFND SWSSFLMDVL SPLSFIKVSH VRMQGILLLV
110 120 130 140 150
FAKYQHLPYI QILSTKSTPT GLFGYWGNKG GVNICLKLYG YYVSIINCHL
160 170 180 190 200
PPHISNNYQR LEHFDRILEM QNCEGRDIPN ILDHDLIIWF GDMNFRIEDF
210 220 230 240 250
GLHFVRESIK NRCYGGLWEK DQLSIAKKHD PLLREFQEGR LLFPPTYKFD
260 270 280 290 300
RNSNDYDTSE KKRKPAWTDR ILWRLKRQPC AGPDTPIPPA SHFSLSLRGY
310 320 330 340 350
SSHMTYGISD HKPVSGTFDL ELKPLVSAPL IVLMPEDLWT VENDMMVSYS
360 370 380 390 400
STSDFPSSPW DWIGLYKVGL RDVNDYVSYA WVGDSKVSCS DNLNQVYIDI
410 420 430 440
SNIPTTEDEF LLCYYSNSLR SVVGISRPFQ IPPGSLREDP LGEAQPQI
Length:448
Mass (Da):51,090
Last modified:October 17, 2006 - v3
Checksum:i46FAA48C6E2EEAD4
GO
Isoform 21 Publication (identifier: Q9BT40-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Show »
Length:372
Mass (Da):42,784
Checksum:iE257B4890DCD44F3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti120 – 1201T → A in AAB03214. 1 PublicationCurated
Sequence conflicti416 – 4161S → R in BAA92340. (PubMed:10753883)Curated
Sequence conflicti416 – 4161S → R in BAA92341. (PubMed:10753883)Curated
Sequence conflicti416 – 4161S → R in BAA92342. (PubMed:10753883)Curated
Sequence conflicti416 – 4161S → R in AAB03214. 1 PublicationCurated
Sequence conflicti426 – 4261S → R in BAA92340. (PubMed:10753883)Curated
Sequence conflicti426 – 4261S → R in BAA92341. (PubMed:10753883)Curated
Sequence conflicti426 – 4261S → R in BAA92342. (PubMed:10753883)Curated
Sequence conflicti426 – 4261S → R in AAB03214. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti315 – 3151S → F in a breast cancer sample; somatic mutation. 1 Publication
VAR_036497

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7676Missing in isoform 2. 2 PublicationsVSP_050612Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB036829 mRNA. Translation: BAA92340.1.
AB036830 mRNA. Translation: BAA92341.1.
AB036831 Genomic DNA. Translation: BAA92342.1.
AK312585 mRNA. Translation: BAG35479.1.
AK312844 mRNA. Translation: BAG35697.1.
CH471108 Genomic DNA. Translation: EAW90614.1.
CH471108 Genomic DNA. Translation: EAW90615.1.
CH471108 Genomic DNA. Translation: EAW90618.1.
BC004362 mRNA. Translation: AAH04362.1.
U45973 mRNA. Translation: AAB03214.1.
CCDSiCCDS11004.1. [Q9BT40-1]
CCDS11005.1. [Q9BT40-2]
RefSeqiNP_001129114.1. NM_001135642.1. [Q9BT40-2]
NP_057616.2. NM_016532.3. [Q9BT40-1]
NP_570122.1. NM_130766.2. [Q9BT40-2]
XP_005256740.1. XM_005256683.1. [Q9BT40-2]
XP_006721608.1. XM_006721545.1. [Q9BT40-2]
UniGeneiHs.632238.

Genome annotation databases

GeneIDi51763.
KEGGihsa:51763.
UCSCiuc002fsq.3. human. [Q9BT40-1]

Polymorphism databases

DMDMi116242791.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB036829 mRNA. Translation: BAA92340.1 .
AB036830 mRNA. Translation: BAA92341.1 .
AB036831 Genomic DNA. Translation: BAA92342.1 .
AK312585 mRNA. Translation: BAG35479.1 .
AK312844 mRNA. Translation: BAG35697.1 .
CH471108 Genomic DNA. Translation: EAW90614.1 .
CH471108 Genomic DNA. Translation: EAW90615.1 .
CH471108 Genomic DNA. Translation: EAW90618.1 .
BC004362 mRNA. Translation: AAH04362.1 .
U45973 mRNA. Translation: AAB03214.1 .
CCDSi CCDS11004.1. [Q9BT40-1 ]
CCDS11005.1. [Q9BT40-2 ]
RefSeqi NP_001129114.1. NM_001135642.1. [Q9BT40-2 ]
NP_057616.2. NM_016532.3. [Q9BT40-1 ]
NP_570122.1. NM_130766.2. [Q9BT40-2 ]
XP_005256740.1. XM_005256683.1. [Q9BT40-2 ]
XP_006721608.1. XM_006721545.1. [Q9BT40-2 ]
UniGenei Hs.632238.

3D structure databases

ProteinModelPortali Q9BT40.
SMRi Q9BT40. Positions 20-323.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119720. 20 interactions.
IntActi Q9BT40. 13 interactions.
MINTi MINT-1445023.
STRINGi 9606.ENSP00000254712.

PTM databases

PhosphoSitei Q9BT40.

Polymorphism databases

DMDMi 116242791.

Proteomic databases

MaxQBi Q9BT40.
PaxDbi Q9BT40.
PRIDEi Q9BT40.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 51763.
KEGGi hsa:51763.
UCSCi uc002fsq.3. human. [Q9BT40-1 ]

Organism-specific databases

CTDi 51763.
GeneCardsi GC17M001397.
HGNCi HGNC:33882. INPP5K.
HPAi HPA031044.
MIMi 607875. gene.
neXtProti NX_Q9BT40.
PharmGKBi PA164720951.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5411.
GeneTreei ENSGT00760000119075.
HOGENOMi HOG000046051.
HOVERGENi HBG082135.
InParanoidi Q9BT40.
KOi K01106.
OMAi GLYGYWG.
PhylomeDBi Q9BT40.
TreeFami TF317034.

Enzyme and pathway databases

BioCyci MetaCyc:HS05626-MONOMER.
Reactomei REACT_121025. Synthesis of PIPs at the plasma membrane.

Miscellaneous databases

GeneWikii SKIP.
GenomeRNAii 51763.
NextBioi 55878.
PROi Q9BT40.
SOURCEi Search...

Gene expression databases

Bgeei Q9BT40.
CleanExi HS_INPP5K.
ExpressionAtlasi Q9BT40. baseline and differential.
Genevestigatori Q9BT40.

Family and domain databases

Gene3Di 3.60.10.10. 1 hit.
InterProi IPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
[Graphical view ]
Pfami PF03372. Exo_endo_phos. 1 hit.
[Graphical view ]
SMARTi SM00128. IPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF56219. SSF56219. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel inositol polyphosphate 5-phosphatase."
    Ijuin T., Mochizuki Y., Fukami K., Funaki M., Asano T., Takenawa T.
    J. Biol. Chem. 275:10870-10875(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Testis1 Publication.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain and Thymus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: EyeImported.
  5. Nussbaum R.L.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-448.
    Tissue: BrainImported.
  6. "Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase SKIP localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal growth factor stimulation."
    Gurung R., Tan A., Ooms L.M., McGrath M.J., Huysmans R.D., Munday A.D., Prescott M., Whisstock J.C., Mitchell C.A.
    J. Biol. Chem. 278:11376-11385(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-349; ASP-361; TRP-362 AND TYR-376.
  7. Cited for: VARIANT [LARGE SCALE ANALYSIS] PHE-315.

Entry informationi

Entry nameiINP5K_HUMAN
AccessioniPrimary (citable) accession number: Q9BT40
Secondary accession number(s): B2R6I2
, B2R750, D3DTH8, Q15733, Q9NPJ5, Q9P2R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: October 17, 2006
Last modified: October 29, 2014
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3