Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial

Gene

ALKBH7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as protein hydroxylase; can catalyze auto-hydroxylation at Leu-110 (in vitro), but this activity may be due to the absence of the true substrate (PubMed:25122757). Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death (PubMed:23666923). ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage (PubMed:23666923). Does not display DNA demethylase activity (PubMed:23666923). Involved in fatty acid metabolism (By similarity).By similarity2 Publications

Cofactori

Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi121 – 1211Iron; catalytic1 Publication
Metal bindingi123 – 1231Iron; catalytic1 Publication
Binding sitei165 – 1651Alpha-ketoglutarate
Metal bindingi177 – 1771Iron; catalytic1 Publication
Binding sitei203 – 2031Alpha-ketoglutarate

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB
  • fatty acid metabolic process Source: UniProtKB
  • regulation of lipid storage Source: UniProtKB
  • regulation of mitochondrial membrane permeability involved in programmed necrotic cell death Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Necrosis

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial (EC:1.14.11.-)
Alternative name(s):
Alkylated DNA repair protein alkB homolog 7
Spermatogenesis cell proliferation-related protein
Spermatogenesis-associated protein 11
Gene namesi
Name:ALKBH7
Synonyms:ABH7, SPATA11
ORF Names:UNQ6002/PRO34564
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:21306. ALKBH7.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi110 – 1101L → Q: Does not affect ability to trigger programmed necrosis. 1 Publication

Organism-specific databases

PharmGKBiPA134905040.

Polymorphism and mutation databases

BioMutaiALKBH7.
DMDMi74733083.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2020MitochondrionSequence AnalysisAdd
BLAST
Chaini21 – 221201Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrialPRO_0000239288Add
BLAST

Proteomic databases

MaxQBiQ9BT30.
PaxDbiQ9BT30.
PRIDEiQ9BT30.

Expressioni

Tissue specificityi

Widely expressed, with highest expression in pancreas, followed by spleen, prostate, ovary and placenta.1 Publication

Gene expression databases

BgeeiQ9BT30.
CleanExiHS_ALKBH7.
ExpressionAtlasiQ9BT30. baseline and differential.
GenevisibleiQ9BT30. HS.

Organism-specific databases

HPAiHPA039571.
HPA039872.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi123993. 3 interactions.
IntActiQ9BT30. 1 interaction.
STRINGi9606.ENSP00000245812.

Structurei

Secondary structure

1
221
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi18 – 225Combined sources
Helixi23 – 286Combined sources
Turni29 – 324Combined sources
Beta strandi33 – 364Combined sources
Helixi42 – 5716Combined sources
Beta strandi69 – 7810Combined sources
Helixi83 – 9614Combined sources
Beta strandi105 – 12117Combined sources
Turni125 – 1273Combined sources
Beta strandi130 – 13910Combined sources
Beta strandi141 – 1499Combined sources
Beta strandi154 – 1596Combined sources
Beta strandi164 – 1685Combined sources
Helixi170 – 1734Combined sources
Beta strandi175 – 1795Combined sources
Helixi182 – 1843Combined sources
Beta strandi197 – 2048Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QKBX-ray2.60A/B/C17-215[»]
4QKDX-ray1.35A/B/C17-215[»]
4QKFX-ray1.99A/B/C17-215[»]
ProteinModelPortaliQ9BT30.
SMRiQ9BT30. Positions 17-206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 1993Alpha-ketoglutarate binding1 Publication

Sequence similaritiesi

Belongs to the alkB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG299394.
GeneTreeiENSGT00390000014585.
HOGENOMiHOG000018442.
HOVERGENiHBG054357.
InParanoidiQ9BT30.
KOiK10769.
OMAiSHWDDAI.
OrthoDBiEOG7M0NT9.
PhylomeDBiQ9BT30.
TreeFamiTF314197.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
[Graphical view]
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BT30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTGLLALR TLPGPSWVRG SGPSVLSRLQ DAAVVRPGFL STAEEETLSR
60 70 80 90 100
ELEPELRRRR YEYDHWDAAI HGFRETEKSR WSEASRAILQ RVQAAAFGPG
110 120 130 140 150
QTLLSSVHVL DLEARGYIKP HVDSIKFCGA TIAGLSLLSP SVMRLVHTQE
160 170 180 190 200
PGEWLELLLE PGSLYILRGS ARYDFSHEIL RDEESFFGER RIPRGRRISV
210 220
ICRSLPEGMG PGESGQPPPA C
Length:221
Mass (Da):24,516
Last modified:June 1, 2001 - v1
Checksum:i3BB7DF2242A49949
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891L → P in BAD97056 (Ref. 4) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti191 – 1911R → Q.
Corresponds to variant rs7540 [ dbSNP | Ensembl ].
VAR_048224

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY427650 mRNA. Translation: AAR24624.1.
AY358858 mRNA. Translation: AAQ89217.1.
AK223336 mRNA. Translation: BAD97056.1.
AK311956 mRNA. Translation: BAG34896.1.
AC011491 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69102.1.
BC004393 mRNA. Translation: AAH04393.1.
CCDSiCCDS12163.1.
RefSeqiNP_115682.1. NM_032306.3.
UniGeneiHs.111099.

Genome annotation databases

EnsembliENST00000245812; ENSP00000245812; ENSG00000125652.
GeneIDi84266.
KEGGihsa:84266.
UCSCiuc002meo.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY427650 mRNA. Translation: AAR24624.1.
AY358858 mRNA. Translation: AAQ89217.1.
AK223336 mRNA. Translation: BAD97056.1.
AK311956 mRNA. Translation: BAG34896.1.
AC011491 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69102.1.
BC004393 mRNA. Translation: AAH04393.1.
CCDSiCCDS12163.1.
RefSeqiNP_115682.1. NM_032306.3.
UniGeneiHs.111099.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QKBX-ray2.60A/B/C17-215[»]
4QKDX-ray1.35A/B/C17-215[»]
4QKFX-ray1.99A/B/C17-215[»]
ProteinModelPortaliQ9BT30.
SMRiQ9BT30. Positions 17-206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123993. 3 interactions.
IntActiQ9BT30. 1 interaction.
STRINGi9606.ENSP00000245812.

Polymorphism and mutation databases

BioMutaiALKBH7.
DMDMi74733083.

Proteomic databases

MaxQBiQ9BT30.
PaxDbiQ9BT30.
PRIDEiQ9BT30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000245812; ENSP00000245812; ENSG00000125652.
GeneIDi84266.
KEGGihsa:84266.
UCSCiuc002meo.2. human.

Organism-specific databases

CTDi84266.
GeneCardsiGC19P006372.
HGNCiHGNC:21306. ALKBH7.
HPAiHPA039571.
HPA039872.
MIMi613305. gene.
neXtProtiNX_Q9BT30.
PharmGKBiPA134905040.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG299394.
GeneTreeiENSGT00390000014585.
HOGENOMiHOG000018442.
HOVERGENiHBG054357.
InParanoidiQ9BT30.
KOiK10769.
OMAiSHWDDAI.
OrthoDBiEOG7M0NT9.
PhylomeDBiQ9BT30.
TreeFamiTF314197.

Miscellaneous databases

ChiTaRSiALKBH7. human.
GenomeRNAii84266.
NextBioi73812.
PROiQ9BT30.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BT30.
CleanExiHS_ALKBH7.
ExpressionAtlasiQ9BT30. baseline and differential.
GenevisibleiQ9BT30. HS.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
[Graphical view]
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel human testis overexpressed gene by digital differential display."
    Mo Y., Li L., Lu G.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  5. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  8. Cited for: TISSUE SPECIFICITY.
  9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Human ALKBH7 is required for alkylation and oxidation-induced programmed necrosis."
    Fu D., Jordan J.J., Samson L.D.
    Genes Dev. 27:1089-1100(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-110.
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  13. "The atomic-resolution structure of human Alkb homolog 7 (ALKBH7), a key protein for programmed necrosis and fat metabolism."
    Wang G., He Q., Feng C., Liu Y., Deng Z., Qi X., Wu W., Mei P., Chen Z.
    J. Biol. Chem. 289:27924-27936(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 17-215 IN COMPLEX WITH 2-OXOGLUTARIC ACID AND MANGANESE, FUNCTION, COFACTOR, SUBUNIT.

Entry informationi

Entry nameiALKB7_HUMAN
AccessioniPrimary (citable) accession number: Q9BT30
Secondary accession number(s): B2R4U9, Q53FF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: June 1, 2001
Last modified: June 24, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was initially reported to localize both in cytoplasm and nucleus (PubMed:17979886). However, it was later shown it localizes in mitochondrion (PubMed:23666923). The discrepancy is probably due to the use of a fusion protein with an N-terminal tag in the initial report (PubMed:17979886).2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.